Individual Animal Data for Methylation- Sensitive Restriction Digestion, followed

by Arbitrarily-Primed PCR (AP-PCR) and Capillary Eletrophoresis, for

B6C3F1 and C57BL/6 Mice, 2 and 4 Week Control and 0.05% (w/w) PB

Treatment Groups

Supplementary Fig. S1

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RsaI/HpaII Digest: B6C3F1, 2 Week Control Individual Animal Data

Supplementary Figure S2. Individual animal data for the B6C3F1, 2 week control group (n = 6) after RsaI/HpaII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/HpaII Digest: B6C3F1, 2 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S3. Individual animal data for the B6C3F1, 2 week 0.05% PB-treated group (n = 6) after RsaI/HpaII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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C57-1 C57-2 C57-3 C57-4 C57-5 C57-6 C57-7

RsaI/HpaII Digest: C57BL/6, 2 Week Control Individual Animal Data

Supplementary Figure S4. Individual animal data for the C57BL/6, 2 week control group (n = 7) after RsaI/HpaII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/HpaII Digest: C57BL/6, 2 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S5. Individual animal data for the C57BL/6, 2 week 0.05% PB-treated group (n = 6) after RsaI/HpaII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/HpaII Digest: B6C3F1, 4 Week Control Individual Animal Data

Supplementary Figure S6. Individual animal data for the B6C3F1, 4 week control group (n = 7) after RsaI/HpaII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/HpaII Digest: B6C3F1, 4 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S7. Individual animal data for the B6C3F1, 4 week 0.05% PB-treated group (n = 6) after RsaI/HpaII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/HpaII Digest: C57BL/6, 4 Week Control Individual Animal Data

Supplementary Figure S8. Individual animal data for the C57BL/6, 4 week control group (n = 7) after RsaI/HpaII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/HpaII Digest: C57BL/6, 4 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S9. Individual animal data for the C57BL/6, 4 week 0.05% PB-treated group (n = 6) after RsaI/HpaII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/MspI Digest: B6C3F1, 2 Week Control Individual Animal Data

Supplementary Figure S10. Individual animal data for the B6C3F1, 2 week control group (n = 6) after RsaI/MspI digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/MspI Digest: B6C3F1, 2 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S11. Individual animal data for the B6C3F1, 2 week 0.05% PB-treated group (n = 6) after RsaI/MspI digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/MspI Digest: C57BL/6, 2 Week Control Individual Animal Data

Supplementary Figure S12. Individual animal data for the C57BL/6, 2 week control group (n = 7) after RsaI/MspI digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/MspI Digest: C57BL/6, 2 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S13. Individual animal data for the C57BL/6, 2 week 0.05% PB-treated group (n = 6) after RsaI/MspI digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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B6-1 B6-2 B6-3 B6-4 B6-5 B6-6 B6-7

RsaI/MspI Digest: B6C3F1, 4 Week Control Individual Animal Data

Supplementary Figure S14. Individual animal data for the B6C3F1, 4 week control group (n = 7) after RsaI/MspI digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/MspI Digest: B6C3F1, 4 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S15. Individual animal data for the B6C3F1, 4 week 0.05% PB-treated group (n = 6) after RsaI/MspI digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/MspI Digest: C57BL/6, 4 Week Control Individual Animal Data

Supplementary Figure S16. Individual animal data for the C57BL/6, 4 week control group (n = 7) after RsaI/MspI digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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RsaI/MspI Digest: C57BL/6, 4 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S17. Individual animal data for the C57BL/6, 4 week 0.05% PB-treated group (n = 6) after RsaI/MspI digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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BfaI/BssHII Digest: B6C3F1, 2 Week Control Individual Animal Data

Supplementary Figure S18. Individual animal data for the B6C3F1, 2 week control group (n = 6) after BfaI/BssHII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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BfaI/BssHII Digest: B6C3F1, 2 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S19. Individual animal data for the B6C3F1, 2 week 0.05% PB-treated group (n = 6) after BfaI/BssHII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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BfaI/BssHII Digest: C57BL/6, 2 Week Control Individual Animal Data

Supplementary Figure S20. Individual animal data for the C57BL/6, 2 week control group (n = 7) after BfaI/BssHII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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BfaI/BssHII Digest: C57BL/6, 2 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S21. Individual animal data for the C57BL/6, 2 week 0.05% PB-treated group (n = 6) after BfaI/BssHII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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BfaI/BssHII Digest: B6C3F1, 4 Week Control Individual Animal Data

Supplementary Figure S22. Individual animal data for the B6C3F1, 4 week control group (n = 7) after BfaI/BssHII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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BfaI/BssHII Digest: B6C3F1, 4 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S23. Individual animal data for the B6C3F1, 4 week 0.05% PB-treated group (n = 6) after BfaI/BssHII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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BfaI/BssHII Digest: C57BL/6, 4 Week Control Individual Animal Data

Supplementary Figure S24. Individual animal data for the C57BL/6, 4 week control group (n = 7) after BfaI/BssHII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.

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BfaI/BssHII Digest: C57BL/6, 4 Week 0.05% PB-treated Individual Animal Data

Supplementary Figure S25. Individual animal data for the C57BL/6, 4 week 0.05% PB-treated group (n = 6) after BfaI/BssHII digestion, followed by arbitrarily-primed PCR (AP-PCR) and capillary electrophoresis, are shown. The X-axis represents the size of the PCR products that formed, and the Y-axis represents the amount of each PCR product. Each color represents a different animal within the group, and each dot represents the average of 2 duplicate AP-PCR reactions.