Supplementary Fig. 1
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Supplementary Fig. 1 Total glycolipids were extracted from C. glutamicum wild type (WT) and the ∆NCgl1054 mutant and analysed by HPTLC. Glycolipids were developed in chloroform/methanol/1 M ammonium acetate/13M ammonium solution/H 2 O (180:140:9:9:2.3,v/v) and detected with orcinol-H 2 SO 4 staining. The HPTLC mobility of trehalose dicorynomycolate (TDCM), trehalose monocorynomycolate (TMCM), Gl- X and AcPIM2 are shown. Note accumulation of Gl-X and AcPIM2 in the mutant. C. g l u tamic u m W T C.gl ut amic um ∆NC gl1054 AcPIM2 Gl-X TMCM TDCM origin front
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Supplementary Fig. 1. - PowerPoint PPT Presentation
Transcript of Supplementary Fig. 1
Supplementary Fig. 1
Total glycolipids were extracted from C. glutamicum wild type (WT) and the ∆NCgl1054 mutant and analysed by HPTLC. Glycolipids were developed in chloroform/methanol/1 M ammonium acetate/13M ammonium solution/H2O (180:140:9:9:2.3,v/v) and detected with orcinol-H2SO4 staining. The HPTLC mobility of trehalose dicorynomycolate (TDCM), trehalose monocorynomycolate (TMCM), Gl-X and AcPIM2 are shown. Note accumulation of Gl-X and AcPIM2 in the mutant.
C.gl
utam
icum
WT
C.gl
utam
icum
∆NCg
l105
4
AcPIM2Gl-X
TMCM
TDCM
origin
front