Supplementary Fig. 1

3
Supplementary Fig. 1 cental RLN3 ancestor supial RLN3 otreme RFLCI hibian RFLCI eost RFLC ancestor RFLC (alternative) GHSIYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC YPSFYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC YPSFYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC DDPIYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC AGPSYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKGEISSLC RVPTFGVKLCGTEFIRAVIFTCGGSRW EAALGLSKTCCKWGCSKSQISSLC RVPTFGVKLCGREFIRAVIFTCGGSRW EAALGLSNTCCKWGCSKSQISSLC RPSTYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC RAAPYGVKLCGREFIRAVIFTCGGSRW DVLAGLSSSCCKWGCSKSEISSLC RTSPYAVKLCGREFIRAVIFTCGGSRW DVLAGVSSNCCKWGCSKTEISSLC RSPSYGMKLCGREFIRAVIFTCGGSRW DVLAGLSSNCCKWGCSKSEISSLC RVPTFGVKLCGREFIRAVIFTCGGSRW EAALGLSNTCCKWGCSKSQISSLC DDPIYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC RAPTYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC A chain B chain . rerio rubripes nigroviridis rubripes . rerio laevis tropicalis ncRFLC RFLC1 RFLC1 RFLC1 RFLC2 RFLC2 RFLCI RFLCI

description

D. rerio T. rubripes T. nigroviridis T. rubripes D. rerio X. laevis X. tropicalis AncRFLC. RFLC1 RFLC1 RFLC1 RFLC2 RFLC2 RFLCI RFLCI. A chain. B chain. GHSIYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC YPSFYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC - PowerPoint PPT Presentation

Transcript of Supplementary Fig. 1

Page 1: Supplementary Fig. 1

Supplementary Fig. 1

Placental RLN3 ancestorMarsupial RLN3Monotreme RFLCIAmphibian RFLCITeleost RFLC ancestor

AncRFLC (alternative)

GHSIYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLCYPSFYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLCYPSFYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLCDDPIYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLCAGPSYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKGEISSLCRVPTFGVKLCGTEFIRAVIFTCGGSRW EAALGLSKTCCKWGCSKSQISSLCRVPTFGVKLCGREFIRAVIFTCGGSRW EAALGLSNTCCKWGCSKSQISSLC

RPSTYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC

RAAPYGVKLCGREFIRAVIFTCGGSRW DVLAGLSSSCCKWGCSKSEISSLCRTSPYAVKLCGREFIRAVIFTCGGSRW DVLAGVSSNCCKWGCSKTEISSLCRSPSYGMKLCGREFIRAVIFTCGGSRW DVLAGLSSNCCKWGCSKSEISSLCRVPTFGVKLCGREFIRAVIFTCGGSRW EAALGLSNTCCKWGCSKSQISSLCDDPIYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC

RAPTYGVKLCGREFIRAVIFTCGGSRW DVVVGLSNACCKWGCSKSEISSLC

A chainB chainD. rerio T. rubripes T. nigroviridisT. rubripes D. rerio X. laevis X. tropicalis

AncRFLC

RFLC1RFLC1RFLC1RFLC2RFLC2RFLCIRFLCI

Page 2: Supplementary Fig. 1

zRFLC

1zR

FLC1

RB12HzR

FLC

2zR

FLC2

RB12HAncR

FL

CAncR

FLC

RB12H

RLN3 RB12 H

RLN3

RLN3 RB12 A

RLN3 FB20 R

RLN3 SB25 P

INSL3

INSL3 H

B12 R

INSL3 H

B12 A

AncRFLC

AncRFLC V

VA3,4 LA

AncRFLC N

AA8,9 SS

RLN3 LA

A3,4 VV

RLN3 SSA8,9 NA

RLN3

RLN3

oINSL3

INSL3

pRFLCI

pRFLCII

4

66

10

4

66

10

kDa kDa kDa

kDa kDa kDa

Nonreducing

4

66

10

Nonreducing

Reducing

4

66

10

Reducing4

66

10

4

66

10

Supplementary Fig. 2

Page 3: Supplementary Fig. 1

Human INSL3

Platypus RFLCII

Supplementary Fig. 3

Therian mammals

Monotreme

Human INSL3Chimp INSL3Mouse Insl3Rat Insl3Cow INSL3Dog INSL3Opossum INSL3

Platypus RFLCII

E K L C G H H F V R A L VGAGAAGTTGTGCGGCCACCACTTCGTACGCGCGCTAGTGGAGAAGTTGTGCGGCCACCACTTCGTACGCGCGCTGGTGGCCAAGCTCTGCGGCCACCACCTGGTGCGCACGCTGGTGGCCAAGCTCTGTGGTCACCACCTGGTGCGTGCGCTGGTGGAGAAACTGTGTGGCCACCACTTCGTGCGCGCGCTCGTGGAGAAGCTGTGTGGCCACCACTTCGTGCGGGCGCTGGTGCAGAAACTCTGTGCCCACCGCTTCATCCGGGCCCTCGTC

GTGAAGCTGTGTGGGCGGGAGTTCATCCGTGCTCTGATTV K L C G R E F I R A L I