Holly Sewell 23 July 2010
description
Transcript of Holly Sewell 23 July 2010
Holly Sewell23 July 2010
Marine Bioluminescen
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Marine Bioluminescence
www.ou.edu
www.ou.edu
Experiment
Organisms and EnvironmentsWhere is it found?
FunctionProposed explanations
GenesReaction
Experiment: Can we isolate bioluminescent colonies?
SWC SWC
Bacterial BioluminescenceWhere is it found?
Photobacterium
www.bioart.co.uk
Vibrio
www.alchemipedia.com
Photorhabdus
www.asm.com
Some colonize light organs, producing light for animalfor signalingavoiding predatorsattracting prey
Parasites in crustacea
Estimated 1-6x103 free-living bioluminescent bacteria per liter of water
Proposed explanation for bacterial bioluminescence Anaerobic bacteria may have developed bioluminescence to use up toxic oxygen
(Tett & Kelley, 1973)
Catalyze the transfer of electrons to oxygen (Nealson & Hastings, 1979)
High population density quorum sensing: attract animals to feed dispersal of population(Microbiology, Brock)
Stimulation of DNA repair (Czyz et. al, 2000)
www.pubs.acs.org
Bioluminescence Genes
lux I
AI
AIAIAI
AI
AI
AI
lux R
AI
AI
AI
AIActivation of luminescence genes
Bioluminescence reaction:How it works
ATP
AMP + PPi
RCOOH
RCHO
Oxidoreductase
NAD(P)H + H+
NAD(P)+
NAD(P)H + H+
NAD(P)+
Fatty AcidReductase
Luciferase
O2
Light
FMNH2 + O2 + RCHO FMN + RCOOH + H2O + LightLuciferase
Questions?
An Example of Bacterial BioluminescenceLight Organ colonization in Euprymna scolopes
Colonized by Vibrio fischeriboth light and presence important for development of squid
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