r Dalbavancin HCl HNMR analysis in DMSO-d6 MedKoo ......7 7.012 VBA 0.0863 8393.50586 1476.84204...

12 11 10 9 8 7 6 5 4 3 2 1 0 ppm

0.842

0.858

1.132

1.144

1.238

1.915

2.504

2.509

2.513

2.708

2.720

3.370

3.375

3.384

3.409

3.495

4.305

4.318

5.067

5.333

5.383

5.404

5.787

6.079

6.104

6.378

6.383

6.583

6.666

6.681

6.713

6.747

6.806

7.075

7.101

7.121

7.146

7.253

7.277

7.297

7.419

7.439

7.698

8.512

9.91

23.4

03.

994.

462.

764.

318.

314.

851.

571.

571.

331.

021.

593.

651.

920.

880.

560.

561.

061.

876.

761.

304.

882.

441.

741.

311.

190.

800.

981.

082.

571.

591.

941.

142.

201.

00

Current Data ParametersNAME Dalbavancin_HClEXPNO 10PROCNO 1F2 - Acquisition Parameters

INSTRUM spectPROBHD 5 mm PABBO BB-PULPROG zg30TD 65536SOLVENT DMSONS 16DS 2SWH 8012.820 HzFIDRES 0.122266 HzAQ 4.0894465 secRG 114DW 62.400 usecDE 6.50 usecTE 298.6 KD1 1.00000000 secTD0 1

======== CHANNEL f1 ========SFO1 400.1324710 MHzNUC1 1HP1 15.00 usecPLW1 33.00000000 W

F2 - Processing parametersSI 65536SF 400.1300000 MHzWDW noSSB 0LB 0 HzGB 0PC 1.00

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MedKooTypewritten TextMedKoo Cat#: 317136Name: Dalbavancin HClCAS#: 171500-79-1 (free base)Lot#: TZC51126Chemical Formula: C88H101Cl3N10O28Molecular Weight: 1853.17

MedKooTypewritten Text

MedKooStamp

MedKooTypewritten TextDalbavancin HCl HNMR analysis in DMSO-d6

=====================================================================: ZJ User

InstrumentSample Name

: Instrument 1: Dalbavancin-HCl: C:\CHEM32\1\DATA\TEST-KR.M

: C:\CHEM32\1\METHODS\TEST-KR.M

min2 4 6 8 10 12 14 16

mAU

0

200

400

600

800

1000

1200

DAD1 A, Sig=214,4 Ref=off

3.1

54

5.4

18 5

.705

6.4

506.

797

6.8

84 7

.012

Signal 1: DAD1 A, Sig=214,4 Ref=off

Peak# Ret.Time Type Width Area Height Area # [min] [min] [mAU*s] [mAU] %----|-------|----|-------|----------|----------|--------| 1 3.154 BBA 0.0460 78.81187 27.02951 0.8661 2 5.418 BB 0.0720 395.37540 82.32384 4.3450 3 5.705 BB 0.0745 28.49776 5.68175 0.3132 4 6.450 BV 0.0616 67.87001 17.37079 0.7459 5 6.797 BV 0.0466 64.01775 22.89089 0.7035 6 6.884 VV 0.0501 71.51752 24.43673 0.7859 7 7.012 VBA 0.0863 8393.50586 1476.84204 92.2404

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Seq. Line : 1 Location : Vial 17 Inj : 1 Inj Volume : 1.0 µlAcq. Method

Analysis Method

=====================================================================Area Percent Report

=====================================================================

:Sorted By Signal 1.0000:Multiplier 1.0000:Dilution

Use Multiplier & Dilution Factor with ISTDs

9099.59616 :Total 1656.57555






MedKooTypewritten TextDalbavancin HCl HPLC analysis



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MS Spectrum

m/z600 800 1000 1200 1400

0

20

40

60

80

100

*MSD1 SPC, ES-API, Po

Max: 113076

121

0.7

908

.3 9

07.8

909

.2 9

08.7

=====================================================================

Seq. Line : 6 Location : Vial 100

Acq. Operator : [BSB2] Acq. Instrument : Instrument 1 Sample Name : Dalbavancin -HCL Inj : 1

Inj Volume : 1.000 µlAcq. Method : C:\CHEM32\1\DATA\TEST-1-POS-KR.M

Analysis Method : C:\CHEM32\1\METHODS\TEST-1-POS-KR.M

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MedKooTypewritten TextDalbavancin freebaseChemical Formula: C88H100Cl2N10O28 Exact Mass: 1814.60856 Molecular Weight: 1816.71000

MedKooTypewritten Text(M+2)/2 =(1814+2)/2 = 908



MedKooLine

MedKooTypewritten TextDalbavancin HCl MS analysis



r Dalbavancin HCl HNMR analysis in DMSO-d6 MedKoo ......7 7.012 VBA 0.0863 8393.50586 1476.84204...

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