The effects of biomass burning from Amazon region in human lung
cells
Nilmara Alves Post-doctoral researcher School of Medicine of
University of So Paulo, Brazil Good afternoon, Im Nilmara Alves. Im
a pos-doctor in the School of Medicine of University of So Paulo.
This work is the result of a collaboration between Federal
University of Rio Grande do Norte and my university. The title is
The effects of biomass burning from Amazon region in human lung
cells. First, Ill provide a brief overview of the studied region.
Amazon region The Amazon region occupies more than half of the
Brazilian territory. This vast region includes both clear area and
human impacted areas. The Brazilian Amazon has been affected by
deforestation and biomass burning and these cause important impacts
on the climate and with adverse effects on human health. Effects on
human health
Amazon region Biomass Burning The Amazon region occupies more than
half of the Brazilian territory. This vast region includes both
clear area and human impacted areas. The Brazilian Amazon has been
affected by deforestation and biomass burning and these cause
important impacts on the climate and with adverse effects on human
health. Effects on human health The majority of forest fire
hotspots in the Brazilian Amazon take place in the deforestation
arc with a population of over 10 million people Amazon region The
Amazon region occupies more than half of the Brazilian territory.
This vast region includes both clear area and human impacted areas.
The Brazilian Amazon has been affected by deforestation and biomass
burning and these cause important impacts on the climate and with
adverse effects on human health. The majority of forest fire
hotspots in the Brazilian Amazon take place in the deforestation
arc with a population of over 10 million people Brazilian Amazon
Amazon region The Amazon region occupies more than half of the
Brazilian territory. This vast region includes both clear area and
human impacted areas. The Brazilian Amazon has been affected by
deforestation and biomass burning and these cause important impacts
on the climate and with adverse effects on human health. The
majority of forest fire hotspots in the Brazilian Amazon take place
in the deforestation arc with a population of over 10 million
people Brazilian Amazon Amazon region The Amazon region occupies
more than half of the Brazilian territory. This vast region
includes both clear area and human impacted areas. The Brazilian
Amazon has been affected by deforestation and biomass burning and
these cause important impacts on the climate and with adverse
effects on human health. Deforestation arc It is important to note
that the World Health Organization classified outdoor air pollution
as carcinogenic to humans. Despite the effort from epidemiological
studies to investigate the effects of the biomass burning on human
health, there is not single definitive mechanism of action that
explains those results. Particulate matter smaller than 10 m
This is the first work that investigates the mechanism of action of
PM emitted by biomass burning in the Amazon region. Sampling site
To collect the samples we used a high volume sampler and we
collected the particulate matter smaller than 10 microns because
these are the inhalable particles. There are few works that study
the mechanisms of action of atmospheric pollutants. As far as we
know this is the first work that aims to investigate the pathways
of action of organic particulate in the Amazon region. So, the
organic matter was extracted and before investigating the
mechanisms of action, we identified the compounds in this complex
mixture. High Volume Sampler Particulate matter smaller than 10 m
Polycyclic Aromatic Hydrocarbons (PAH)
After collecting, we identified many compounds such as polycyclic
aromatic hydrocarbons. Most of the identified compounds are
mutagenic and carcinogenic. For example, benzo-a-pirene. Most of
the identified PAH are mutagenic and carcinogenic. De Oliveira
Alves et al., 2015 Atmospheric Environment Viability Test XTT
Kit
- Human lung cells (A549 cell line) * ** After the chemical
analysis, we performed the viability test using A549 cells under
different concentrations. We observed that the organic particulate
matter altered the cell viability after 72 h of exposure at higher
concentration. DC: DMSO 0.1% control PAHs: Polycyclic Aromatic
Hydrocarbons (30 ng/mL) *p
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