The effects of biomass burning from Amazon region in human lung cells
Nilmara Alves Post-doctoral researcher School of Medicine of University of So Paulo, Brazil Good afternoon, Im Nilmara Alves. Im a pos-doctor in the School of Medicine of University of So Paulo. This work is the result of a collaboration between Federal University of Rio Grande do Norte and my university. The title is The effects of biomass burning from Amazon region in human lung cells. First, Ill provide a brief overview of the studied region. Amazon region The Amazon region occupies more than half of the Brazilian territory. This vast region includes both clear area and human impacted areas. The Brazilian Amazon has been affected by deforestation and biomass burning and these cause important impacts on the climate and with adverse effects on human health. Effects on human health
Amazon region Biomass Burning The Amazon region occupies more than half of the Brazilian territory. This vast region includes both clear area and human impacted areas. The Brazilian Amazon has been affected by deforestation and biomass burning and these cause important impacts on the climate and with adverse effects on human health. Effects on human health The majority of forest fire hotspots in the Brazilian Amazon take place in the deforestation arc with a population of over 10 million people Amazon region The Amazon region occupies more than half of the Brazilian territory. This vast region includes both clear area and human impacted areas. The Brazilian Amazon has been affected by deforestation and biomass burning and these cause important impacts on the climate and with adverse effects on human health. The majority of forest fire hotspots in the Brazilian Amazon take place in the deforestation arc with a population of over 10 million people Brazilian Amazon Amazon region The Amazon region occupies more than half of the Brazilian territory. This vast region includes both clear area and human impacted areas. The Brazilian Amazon has been affected by deforestation and biomass burning and these cause important impacts on the climate and with adverse effects on human health. The majority of forest fire hotspots in the Brazilian Amazon take place in the deforestation arc with a population of over 10 million people Brazilian Amazon Amazon region The Amazon region occupies more than half of the Brazilian territory. This vast region includes both clear area and human impacted areas. The Brazilian Amazon has been affected by deforestation and biomass burning and these cause important impacts on the climate and with adverse effects on human health. Deforestation arc It is important to note that the World Health Organization classified outdoor air pollution as carcinogenic to humans. Despite the effort from epidemiological studies to investigate the effects of the biomass burning on human health, there is not single definitive mechanism of action that explains those results. Particulate matter smaller than 10 m
This is the first work that investigates the mechanism of action of PM emitted by biomass burning in the Amazon region. Sampling site To collect the samples we used a high volume sampler and we collected the particulate matter smaller than 10 microns because these are the inhalable particles. There are few works that study the mechanisms of action of atmospheric pollutants. As far as we know this is the first work that aims to investigate the pathways of action of organic particulate in the Amazon region. So, the organic matter was extracted and before investigating the mechanisms of action, we identified the compounds in this complex mixture. High Volume Sampler Particulate matter smaller than 10 m Polycyclic Aromatic Hydrocarbons (PAH)
After collecting, we identified many compounds such as polycyclic aromatic hydrocarbons. Most of the identified compounds are mutagenic and carcinogenic. For example, benzo-a-pirene. Most of the identified PAH are mutagenic and carcinogenic. De Oliveira Alves et al., 2015 Atmospheric Environment Viability Test XTT Kit
- Human lung cells (A549 cell line) * ** After the chemical analysis, we performed the viability test using A549 cells under different concentrations. We observed that the organic particulate matter altered the cell viability after 72 h of exposure at higher concentration. DC: DMSO 0.1% control PAHs: Polycyclic Aromatic Hydrocarbons (30 ng/mL) *p