Types of Microscopes

Unit 3 – Powerpoint #1Honors Biology

2012-2013

Use a beam of LightThe specimen can be dead or alive

Light Microscopes

Have only 1 lensGive two examples of a simple microscope:

Simple Microscopes

•Magnifying Glass• Reading Glasses

Simple Microscope

Compound Microscopes

Have more than one ______

lens

Dissecting Microscopes Magnify by 10x or ______ or ________

Shine light on or ____________ the specimen

Specimen can be thick or _______.

20x 30xthrough

thin

Dissecting Microscope

Light Microscope Magnify by ______________

Shines light ______________ the specimen

Specimen must be _________.

40- 400x

thin

through

Light Microscope Image

Electron MicroscopeUse a beam of _______________-The specimen must be _________ because an electron microscope is used in a vacuum with no _____ and no ________

electronsdead

airwater

Scanning E.M.Makes 3D pictures of surfaces

Magnify by _________60,000x

Scanning E.M. Image (3D)

Transmission E.M.

Makes _____ pictures of thin slices

Magnify by __________100,000x

2D

Transmission E.M. Image (2D)

Butterfly Antenna

Probe MicroscopesIon Microscopes◦_____________ can be seen with these 2 types of microscopes.

Other Microscopes

Atoms

Probe Microscope

Probe Microscope Image Horse Hair

Ion Microscopes

Ion Microscope Images

Microscope Parts and Functions

1. Eyepiece

Magnifies by 10x

2. Revolving Nose Piece

Changes Objective

Lens

3. Low power Objective Lens

Magnifies by 4x

(Red)

4. Medium Power Objective Lens

Magnifies by 10x (yellow)

5. High Power Objective Lens

Magnifies by 40x (Blue)

6. Stage Clips

To hold slide in place

7. Diaphragm

Adjusts the amount of

light

8. Light

Light to see specimen

9. On/Off SwitchTurns light on and off

10. Arm

To hold while

carrying

11. Stage

To set slide on

To focus only when in Low

Power

12. Course Adjustment

13. Fine Adjustment

To focus when using medium or high power

14. Base

•To hold while carrying

A. Carry a microscope with ___________ hands – one hand holding the _______

And the other hand under the ___________

Always hold the microscope upright so the _____________ cannot fall out

When you place a microscope at your table, make sure it is _______ from the edge

If there is anything wrong with the microscope, or it is damaged in any way, let the

_______________ know immediately.

B. To clean the objective lenses and eyepiece, use _______________________

Both ArmBase

Eyepiece

Away

Teacher

Lens Tissue

1. Prepare your slide

2. Plug the microscope in. Turn on the ______________

3. Be sure the ___________ Power Objective Lens (_______) is clicked into place.

NOTE: Be sure to look at the microscope from the side when changing objective

lenses to avoid damaging the ________________ and _____________

4. Use coarse focus to raise the stage to its ________________ position

5. Place the _______________ on the stage.

6. Look through the eyepiece and turn the __________________ (lowering the stage)

until the specimen is visible.

7. Use the ____________ adjustment to bring your specimen into sharp focus.

You may wish to move the ________________ to adjust the amount of light.

Light

Low 4X

Slide Lens

Highest

SlideCoarse Focus

Fine

Diaphragm

8. Make sure the specimen is ______________

9. Switch to the ___________ Power Objective Lens ( ______)

NOTE: Look at the microscope from the ____________ when you change lenses.

10. To avoid losing your focus and/or crushing the slide with the lens, never use the

__________________ focus on medium or high power.

Use the _____________ focus only to make the specimen visible.

11. Make sure the specimen is ______________. Look from the _________ as you

change objective lenses and switch to the __________ Power Lens (________)

12. Use only the _____________ focus to make the specimen visible.

13. When finished, make sure the ___________ power objective lens is in place,

wrap the __________ around the _________ of the microscope, cover, and place

in the appropriate location

Focused

Mid 10XSide

Coarse

Fine

Focused SideHigh 40X

FineLow

Cord Arm