The Measurable Difference

New York City Doctor’s Meeting - June 25, 2004

Presented by Jenny Engemann

“… low levels of the antioxidant vitamins (vitamins A, E, and C) may increase risk for several chronic diseases. Most people do not consume an optimal amount of all vitamins by diet alone.”

“It appears prudent for all adults to take vitamin supplements.”

Journal of the American Medical Association June 2002

Robert H. Fletcher, MD, MSc; Kathleen M. Fairfield, MD, DrPH, Harvard UniversityJournal of the American Medical Association 2002; 287: 3127-3129

A Landmark Endorsement

The Need to Supplement

LifePak® Science is Measurable

• First to show antioxidant and cardiovascular benefits in double-blind studies

• 200,000+ published studies on LifePak’s antioxidants

• Nearly 800,000 published studies on all LifePak ingredients

LifePak 40+ Antioxidants

• All 8 forms of natural vitamin E: 300 IU• Buffered vitamin C: 500 mg• Selenium, Copper, Zinc, Manganese• 6 Carotenoids:

– as found in 5-10 fruits & vegetables per day • Over 20 Flavonoids:

– 6 Catechins, Quercetin, naringenin, hesperidin, 3 Soy isoflavones, Over 10 grape seed polyphenols

• Alpha-Lipoic Acid: 30-50 mg

Sources of Free Radicals

• Environmental pollution (smog, ozone, NO2) • Radiation exposure:

– sunlight (UV-light), X-rays, etc.• Smoking:

– 10,000,000,000,000,000 free radicals/cigarette!

• Metabolism in your mitochondria

LifePak Clinical StudyAntioxidant and Cardiovascular Health

42.1 42

43.4

50.9

30

35

40

45

50

55

60

65

Baseline Endpoint

PlaceboLifePak

p < 0.001

331299

335

716

0

200

400

600

800

1000

1200

Baseline Endpoint

PlaceboLifePak

p < 0.001

3.4773.429

6.248

3.305

0

1

2

3

4

5

6

7

8

Baseline Endpoint

PlaceboLifePak

p < 0.001

Smidt CR, Seidehamel RJ, Devaraj S, Jialal I. FASEB Journal Vol.13 No.4, p.A546, March 1999.

LDL Protection

Vitamin E

Beta-carotene

LifePak Antioxidant Capacity (ORAC)Jan 2003

0

500

1000

1500

2000

2500

3000

3500

Recommended daily antioxidant intake (R. Prior, USDA)

Pharmanex BioPhotonic Scanner• Invented by Werner Gellermann, Ph.D. (U. Utah)• Patent rights licensed to Pharmanex• Measures carotenoid antioxidants in skin

– important group of antioxidants– part of the antioxidant defense system in the body– good indicator of your carotenoid antioxidant status

• Based on Raman spectroscopy• Highly specific for carotenoids • Non-invasive and safe• Rapid (3 minutes)

What is Raman Spectroscopy?“Raman” effectIntense monochromatic light (one color only) interacts with molecules that have vibrational energy (e.g., carotenoids) and gets scattered at a higher wavelength (color). Sir C. V. Raman, Nobel Prize in Physics, 1930

Carotenoid molecules shift blue laser light color to green:

from 473 nm to 510 nm

Is The Scanner Accepted Science?• Review paper published in peer-reviewed scientific journal:

– Current Topics in Nutraceutical Research, May 2004 – Download at www.pharmanex.com => What’s New link

Scanner Science is Measurable

• New York Academy of Sciences, Feb 2003• Oxygen Club of California, Feb 2003 and Feb 2004• FASEB/American Society of Nutritional Sciences - April 2003

and April 2004• Gordon Research Conference, Jan 2004• Review paper pending publication in Current Topics in

Nutraceutical Research, May 2004

2004 Data Histogram Mean = 25,40632,948 Subjects

Skin Carotenoid Score

Fre

qu

en

cy

LifePak

5-9 Fruits & Vegetables

Average (2-3 Fruits & Vegetables)Smokers

Obesity

2004 Data: Fruits & Vegetables

31,100

28,560

24,773

21,981

10,000

15,000

20,000

25,000

30,000

35,000

1 or Less 2 to 3 4 to 5 6 or More

Reported Daily Fruit & Vegetable Servings

Ram

an

In

ten

sit

y, C

ou

nts

.

n = 9,336 n = 13,600 n = 6,916 n = 2,796

p < 0.01 p < 0.01 p < 0.01

2004 Data of 32,648 Non-Supplement Users

2004 Data: LifePak Usage

25,39026,236

34,718

29,672

10,000

15,000

20,000

25,000

30,000

35,000

40,000

None Irregular One/Day Two/Day

Reported LifePak Usage

Ram

an

In

ten

sit

y, C

ou

nts

.

n = 4,014n = 1,361n = 958n = 32,941

p < 0.01 p < 0.01 p < 0.01

2004 Data of 39,656 Subjects

Clinical Study - Sep 2002 LifePak Scanner Score

32175

25991

28924

18828

10000

15000

20000

25000

30000

35000

40000

0 4 8 12Time (Weeks)

Ram

an

In

ten

sit

y, C

ou

nts

.

p < 0.001

p < 0.01

p = 0.23

Body Fat - Inverse Correlation

29,236

25,612

20,835

16,603

10,000

15,000

20,000

25,000

30,000

35,000

< 25 25-29 30-39 > 40

Body Mass Index (BMI, kg/m2)

Ram

an

In

tesit

y, C

ou

nts

.

n = 11,588 n = 8,475 n = 422

p < 0.01 p < 0.01 p < 0.01

n = 4,550

2004 Data of 25,035 Subjects Overweight individuals and anyone on a diet should increase antioxidant protection through diet and supplementation.

Carotenoid Correlation Study June 2003

0

10,000

20,000

30,000

40,000

50,000

60,000

0.0 1.0 2.0 3.0 4.0

Total Serum Carotenoids (mg/ml)

Ra

ma

n In

ten

sit

y C

ou

nts

r = 0.78 p < 0.001

Smidt CR, Gellermann W, Zidichouski JA; FASEB Journal 18(4) pt.I:A480, 2004

Carotenoids Predict Antioxidant Status• Recent paper published in the Journal of Nutrition, 2004:

– Carotenoids (blood) are indicator of total antioxidant status – Carotenoids interact with the antioxidant network– Vitamin E (blood) is not a good indicator of antioxidant status

VITAMIN CcycleVITAMIN E

cycle

THIOLcycle

NAD(P)H + H+

NAD(P)+

Lipid–water interface

ROOHROH

PUFA

O2– and other radicals

Metabolism, strenuous exercise,cigarette smoke, pollution

ROO•RO•

The Antioxidant Network

Carotenoids

Carotenoids

The QuestionsWhat Does the Scanner Tell Me About My Health?

“Low levels of the antioxidant vitamins may increase risk for several chronic diseases.” JAMA, June 2002

Recent Antioxidant Studies†

• Lycopene reduces risk of heart disease in women• Vitamins C, E linked to strong reduction in Alzheimer's risk • Multivitamin supplements decrease stroke risk - “SHEEP” study• Vitamin C decreases risk of coronary heart disease in women• Multivitamin supplements decrease infection and improve quality of life• Beta-carotene, vitamin C, selenium associated lower risk of asthma in children

†AJCN 2004;79(1): 47-53; J Archives Neurology 2004;61:82-88; J Nutr 2003 Aug;133(8):2650-4; JACC 2003;42(2):246-252; Ann Intern Med. 2003;138(5):365-71; Am J Resp Crit Care Med 2004;169:393-398.

MarineOmega™

• Everyone needs to balance fatty acid intake

• An excellent source of essential omega-3 fatty acids, EPA and DHA

• Highest grade fish oils • Contains unique krill oil

Essential Fatty Acids• Omega-3

– Alpha-Linolenic Acid (LNA)– Eicosapentaenoic Acid (EPA)– Docosahexaenoic Acid (DHA)– Dietary sources: green leafy vegetables, marine oil, flaxseed

or canola oil

• Omega-6– Linoleic Acid (LA)– Arachidonic Acid (AA)– Dietary sources: sunflower, safflower, corn oil, animal fat,

fried food

Why MarineOmega™?

0

10

20

30

200019001800(-10,000 )

Total Fat

Saturated Fatn-6

n-3

Humans evolved with optimal ratio of omega-6:omega-3 (1:1)

Fatty Acid Metabolism

LNA

Omega-3 (Plant)

Anti-inflammatoryEicosanoids, 3-PG

TriglyceridesCardiac RhythmBrain Function

Omega-3 (Marine)

EPA DHA4 %

Pro-inflammatory2-PG

LA

Omega-6 (Plant) Omega-6 (Animal)

AA

Health Benefits

• Helps Balance Fatty Acid Nutrition• Cardiovascular Health• Joint Health• Immune Health• Skin Health• NEW Brain Health Benefits from

Krill Phospholipids

Scientific Substantiation• Over 100,000 published scientific studies on health

benefits of fish and fish oil (Medline 1966-2004)

Scientific Papers Published on EPA & DHA

0

200

400

600

800

1000

1200

1970 1975 1980 1985 1990 1995 2000 2003

Publication Year

No

. o

f P

aper

s .

Krill Oil (Euphasia pacifica)

Unique Features:• High in phospholipids (40%)• Rich in bioactive EPA and DHA• Naturally contains a novel flavonoid

and carotenoid antioxidants (astaxanthin)

• Zooplankton/crustacean from Antartic Ocean

• Most abundant biomass (<1% harvested)

Krill Oil: High-Activity EPA & DHA

Uniquely efficacious EPA and DHA:

• Highly bioavailable phospholipid form• In unique chemical structure for maximum

efficacy– cell membranes– cardiovascular function– brain & nerve function

Natural “Structured Lipid”

MarineOmega™ Is SafePharmanex® tests for 60 substances:– Heavy metals (Hg, Cd, Pb, Cr) & As: less than 100 ppb– Pesticides:

• DDT, DDD, DDE, HCB, Lindane: each less than 50 ppb• PCBs less than 90 ppb

– 25 Dioxins and Furans: less than 2 ppb total– p-Anisidine Value, Acid Value, Iodine Value, Free Fatty

Acids, Peroxide Value, Cold Test– Cholesterol (< 4 mg/g)– Complete Fatty Acid Profile– Microbiological Testing

Live Better, Longer

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Com

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LifePak Family

MarineOmega

Optimal Health

Free Radical Protection - BioPhotonic Scanner