8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

1/23

H`nsmnr Knjkuay

T`.B. , C.\.Ue., H.\.Ue. & N.@.

UFKCN LNERIX

&

ANCHBN T@NK E!EA

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

2/23

 R`g XJNpia `iaigjzycg

• Gxncpags il eilnetirs ― eiccij prist`gtfe kriups (`ngc) ir cgtna fijs

(cnkjgsfuc)

 ― Bfssiefnhag pritgfj suhujfts ― sfkcn (υ) lnetir

• Bg!jftfij il `iaigjzycg ― "icpagtg# $ir%fjk &grsfij

il nj gjzycg

 ― el npigjzycg ' cfssfjkspgef!e eilnetirs t`ntnaai$ ft ti pgrlirc fts ih

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

3/23

 R`g XJNpia `iaigjzycg

• Naa cuatf'suhujft XJN piaycgrnsgs `n&g eirgsuhujfts

• *netgrfna XJNpia `n&g n++ftfijna υ suhujft• ,ns lujetfij fj hfj+fjk ti pricitgr

 ―

-j hnetgrfn# XJNpia hfj+s n pricitgr vfn υ ― -j gu%nryitgs# XJNpia hfj+s vfn R. eicpagx

• *netgrfna XJNpia fs rgkuantg+ purgay hy υ(fjftfntfij p`nsg)# hut gu%nryitfe XJNpia fs

rgkuantg+ hit` hy t`g R.s nj+ hy &nrfius kgjgrgkuantiry pritgfjs• Nat`iuk` pricitgrs nrg sfcfanr# t`g hnetgrfna

pricitgr tgj+s ti hg `fk`ay eijsgr&g+

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

4/23

XJNT hfjbs spgeflfe pricitgr

sgqugjegs

 Ufkcn lnetirs rgeikjfzg eijsgjsus

->4 njb -57 sgqugjegs

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

5/23

/gjgrna 4ripgrtfgs il υlnetir

• XJN piaycgrnsg `iaigjzycg hfj+s +frgetay ti BJN vfn  fts υsuhujft

• 4ricitgr eijsgjsus sg>ugjeg (hgai$) fs `fk`ay eijsgr&g+ fjhnetgrfn

8g>ugjeg nafkjcgjt

il G. eiaf pricitgrsrg&gna nprg+icfjnjeg ilegrtnfj rgsf+ugs ntpisftfijs '5 nj+ ―27 rgantf&g ti stnrt

pifjt iltrnjserfptfij (

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

6/23

XJN piaycgrnsg pricitgrs

!N"N NNN

#g$fntfij lric eijsgjsus ->4 % -57 sgqugjeg agnbs ti

&gn'gr kgjg g(prgssfij

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

7/23

)netgrfna sfkcn lnetirs

Ufkcn lnetirs nrg *trnjserfptfij lnetirs+

#fllgrgjt sfkcn lnetirs hfjb XJNT njb rgeikjfzg

spgeflfe ->4 %-57 sgqugjegs

,gap cgat #JN ti g(pisg trnjserfptfijna stnrt sftg ist hnetgrfn .n$g cn/ir njb natgrjntg sfkcn lnetirs

Tricitg hrinb e.njkgs fj kgjg g(prgssfij

G. eiaf  sfkcn lnetirs

H. suhtfafs > sfkcn lnetirs !gjgrnaay% hnetgrfn t.nt af$g fj cirg $nrfgb

gj$frijcgjts .n$g cirg sfkcn lnetirs

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

8/23

Ufkcn lnetirs

G. eiaf enj e.iisg hgt&ggj sfkcn lnetirs njb nhiut 574

trnjserfptfij lnetirs ti lfjg tujg fts trnjserfptfijna iutput

  Nj Xgv Cferi \ia. 7;? 22>-2

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

9/23

  8.nt rgkuantgs sfkcn lnetirs

Juchgr il eipfgs pgr egaa 24 cirg t.nj

natgrjntg

Njtf-sfkcn lnetirs hfjb

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

10/23

)netgrfna XJNT juchgrs

0j aik-p.nsg G. eiaf6

?=444 kgjgs

?1444 eirg XJN piaycgrnsg ciageuags

?1544 nrg netf$g nt n tfcg ?>

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

11/23

 R`g natgrjntf&g υ6 lnetir

• ;ist natgrjntf&g sfkcns nrg rgantg+ fjsg>ugjeg nj+ strueturg ti υ 07

• ?j+ +fstfjet typg il υ enaag+ t`g υ6 lncfay•

Bfllgrgjegs hgt$ggj t`g υ lncfafgs ― υ 6 lncfay s`nrgs ji sg>ugjeg `iciaiky $ft`t`g υ 07 lncfay

 ― =`grgns υ 07  `iaigjzycgs enrry iut t`fspriegss il ipgj eicpagx lircntfij ij t`gfri$j# υ 6  `iaigjyzcgs rg>ufrg hit` njgj`njegr nj+ NR4 ti pgrlirc t`fs priegss

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

12/23

1ysfs ir 1ysikgjy

• Ays"s3 -jlgetfij hy p`nkg pri+uegscnjy prikgjy nj+ hrgn%s ipgj (aysgs)t`g `ist hnetgrfuc

• Ays#k$jy3 Nltgr fjlgetfij# t`g p`nkgBJN fjtgkrntgs fjti t`g `ist kgjicgnj+ rgsf+gs t`grg pnssf&gay ― Ji prikgjy

 ― Ji aysfs il t`g `ist ― "nj suhsg>ugjtay aysg (aysikgjy)

• *netgrfip`nkg anch+n enj +i $"%`$r.

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

13/23  Ays"s   Ays#k$jy

\Fj'ue%"#j

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

14/23

•  R`g p`nkg kgjicg fjtgkrntg+ fjti t`g`ist hnetgrfna kgjicg fs n (r#(`nk$.

• *netgrfuc enrryfjk t`g prip`nkg fs n

ays#k$j• 1ysikgjs nrg "))uj$ ti lurt`gr fjlgetfij

hy sfcfanr p`nkg hgenusg t`g p`nkglujetfijs nrg r$(r$ss$' fj trnjs

• Fj'ue%"#j il t`g aysikgj agn+s ti$*e"s"#j il t`g prip`nkg# r$(a"en%"#j ilt`g p`nkg BJN# nj+ ays"s il t`g `ist

hnetgrfuc

Gagcgjts il aysikgjy

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

15/23

/gjgs nrg eaustgrg+ hy lujetfij fjt`g anch+n kgjicg

Xgeichfjntfij Eijtria rgkfijXgpafentfij Aysfs\frus ̀gnb&tnfa

irfkfj

iX 

Tfjt  iA TA  TXC TX  TXG  TX‐tX5 

tA>  tX>  tX8  t

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

16/23

-ccg+fntg gnraytrnjserfptfij

Rrnjserfptfij hyG. eiaf XJN piaycgrnsg fjftfntgs nt strijkpricitgrs TX , TX‖, njb TA , njb tgrcfjntgs nt t‖s.

  tX>  tX8  t

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

17/23

Njtftgrcfjntfij hy J pritgfj agn+s tignray kgjg gxprgssfij

Tfjt  TA  TXC TX  TXG  TX‐tX5 

tA>  tX>  tX8  t

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

18/23

1ytfe ensen+g3 "ri turjs i: eF# 9pritgfj netfij agn+s ti antg kgjg

gxprgssfij

iX 

Tfjt  iA TA  TXC TX  TXG  TX‐tX5 

tA>  tX>  tX8  t

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

19/23

1ntg stnkg il aytfe ensen+g

@fk̀ eijegjtrntfijs il Eri turj iffl TX njb TA .

Nhujbnjt gxprgssfij lric TX‖.

iX 

Tfjt  iA TA  TXC TX  TXG  TX‐tX5 

tA>  tX>  tX8  t

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

20/23

+

1ysikgjy3 "-- nj+ "--- stfcuantggxprgssfij il eF ti cn%g rgprgssir

iX 

Tfjt  iA TA  TXC TX  TXG  TX‐tX5 

tA>  tX>  tX8  t

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

21/23

1ysikgjy3 Xgprgssir turjs i:trnjserfptfij

iX 

Tfjt  iA TA  TXC TX  TXG  TX‐tX5 

tA>  tX>  tX8  t & i

X8 

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

22/23

Xgprgssir strueturg

ipgrntir 

J-tgrcfjus? BJN hfjbfjk= @gafx-Rurj-@gafx citfl 

E-tgrcfjna bicnfj? pritgfj-pritgfj fjtgrnetfij=bfcgrfzntfij njb eiipgrntfvfty

Eijjgetir 

ipgrntir  ipgrntir 

i!i"

ν rgprgssir fs n bfcgr= cijicgr ̀ns 85< ncfji nefbs.

νrgprgssir enj hfjb eiipgrntfvgayti ipgrntir suh-sftgs.

8/17/2019 SIGMA FACTOR & LAMBDA PHAGE CYCLE

23/23

R`njm y#u