Requestor(s ): Dennis Winge Institution: Univ. of Utah
1
Requestor(s): Dennis Winge Institution: Univ. of Utah Request Title: Shy1 from S. cerevisiae # Protein Name MPP/ TMPC ORF ID Target Request ID Source Org. Score pI Resi dues Size (kDa ) Shy1 GO.111729 TR.27140 S. cerevisiae 709509940090000000 9.8 389 45 Alt.names: Cytochrome oxidase assembly protein SHY1, YGR112W, G6150, SURF1 homolog of Yeast, SURF1-like protein Requestor notes (Dennis Winge, from grant support letter): The expression should be done in the absence of residues 1- 50 as that constitutes the mito targeting sequence. Since it is a Cox1 chaperone, it doesn’t have an activity easy to measure. We will check heme binding as one potential assay. We will send the yeast clone. TMPC notes (dja) : Shy1 features a targeting sequence (1-50), predicted TMs (72-92 and 342-362), and a SURF1 domain (77-354). It interacts with COA1, COX14 and MSS51 according to Uniprot, and Cox11, Cox15, Cox6, Cox1, Cox14, and MSS51 according to String. There are no similar structures in the PDB, nor TMPC/MPP OUTSIDE REQUEST FORM Review Date 5/25/11
description
TMPC/MPP OUTSIDE REQUEST FORM. Review Date 5/25/11. Requestor(s ): Dennis Winge Institution: Univ. of Utah Request Title : Shy1 from S. cerevisiae. - PowerPoint PPT Presentation
Transcript of Requestor(s ): Dennis Winge Institution: Univ. of Utah
Requestor(s): Dennis WingeInstitution: Univ. of UtahRequest Title: Shy1 from S. cerevisiae
#Protein Name
MPP/ TMPC
ORF IDTarget
Request ID Source Org. Score pIResidu
esSize
(kDa)
Shy1 GO.111729 TR.27140 S. cerevisiae 709509940090000000 9.8 389 45
Alt.names: Cytochrome oxidase assembly protein SHY1, YGR112W, G6150, SURF1 homolog of Yeast, SURF1-like protein
Requestor notes (Dennis Winge, from grant support letter):
The expression should be done in the absence of residues 1- 50 as that constitutes the mito targeting sequence. Since it is a Cox1 chaperone, it doesn’t have an activity easy to measure. We will check heme binding as one potential assay. We will send the yeast clone.
TMPC notes (dja): Shy1 features a targeting sequence (1-50), predicted TMs (72-92 and 342-362), and a SURF1 domain (77-354). It interacts with COA1, COX14 and MSS51 according to Uniprot, and Cox11, Cox15, Cox6, Cox1, Cox14, and MSS51 according to String. There are no similar structures in the PDB, nor competition for proteins >30% identical. Note that MPP partner Northeast Structural Genomics (NESG) has expressed the soluble domain (aa 110-339) with high ratings for expression and solubility. The clone has been received. Should it be made no-tag as well as tagged for interaction studies?
TMPC/MPP OUTSIDE REQUEST FORMReview Date 5/25/11
