Produzione bio-tecnologica di composti

bioattivi da matrici vegetali e studi di stabilità

per incapsulamento

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S.Caretto

Production of BIOACTIVE COMPOUNDS by plant cell cultures

Enhancement of product yields

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Encapsulation of BIOACTIVE COMPOUNDS obtained by differentplant sources

Improvement of product stability

Plant Cell Cultures: valuable tools forcontrolled production of Bioactive Compounds

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because of

the continuous supply of highly specialized natural compounds that cannot be

produced in equal quality or specificity by chemical synthesis

through

� the manipulation of culture conditions to lead to high

flux into metabolic pathways

� screening and selection of cell lines

� large scale growth in bioreactors

Plant tissue cultures for the production of bioactive compounds

and investigation on their metabolic pathways

Established Plant Cell Cultures

•Helianthus annuus

•Prunus sp.

•Eugenia myrtifolia

•Artemisia annua

Bioactive compounds

•Tocopherols

•Anthocyanins

•Artemisinin

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0

5

10

15

20

25

30

µµ µµg/g

fres

h w

eigh

t

HT LT

TocopherolChlorophyll

HT

Selection of sunflower cell lines with

enhanced vitamin E production

LT

Minutes 0,0 2,5 5,0 7,5 10,0 12,5 15,0 17,5 20,0 22,5 25,0 27,5 30,0

mAU

0

200

400

600

800

1000

mAU

0

200

400

600

800

1000

10,133

11,300

13,133

13,967 12,200

B A

Cherry cell lines producing

anthocyanin

Tempo di trattamento

30 min 1ora 2 ore 4 ore 24 ore 48 ore 5gg

Art

emis

inin

a (µ

g/g

peso

sec

co)

0

4

8

12

16

0 5 10

mAU

0

50

100

150

200

0 5 10

mAU

0

50

100

150

200

MeJA elicited Artemisia cell

cultures

Main publications:

Caretto et al. (2004) Plant Cell Rep.; Gala et al. (2005) J. Plant Physiol.; Blando et al., (2005) Food Res. Int.

Fachechi et al. (2007) Plant Cell Rep.