PERSONAL...31. What regulates mitochondrial DNA copy number in animal cells? Moraes, C. T. Trends...
Transcript of PERSONAL...31. What regulates mitochondrial DNA copy number in animal cells? Moraes, C. T. Trends...
Curriculum Vitae Carlos T. Moraes 1
1. Date: October 20, 2018
PERSONAL
2. Name: Carlos Torres Moraes 3. Home Phone: (305) 856-2662 4. Work Phone: (305) 243-5858 5. Home Address: 2510 Lincoln Avenue, Miami, FL 33133 6. Current Academic Rank: Professor (Esther Lichtenstein Professor) 7. Primary Department: Neurology 8. Secondary Appointment: Cell Biology 9. Citizenship: American/Brazilian (dual)
HIGHER EDUCATION
10. Institutional: Department of Genetics and Development, Columbia University; Ph.D.; February/1993 Department of Genetics and Development, Columbia University; M.A.; February/1991 Department of Biochemistry Escola Paulista de Medicina, São Paulo, S.P. Brazil; M.Sc.; July/1987 Biomedical Sciences Escola Paulista de Medicina, São Paulo, S.P. Brazil; Baccalaureate; December/1983 12. Non-institutional: None 13. Certification, licensure: None
EXPERIENCE
14. Biological Research Training Programs
Structure of glycosaminoglycans and interactions with cellular matrix proteins. Department of Biochemistry - Escola Paulista de Medicina, São Paulo - Brazil M.Sc. candidate; Supervisor: Helena B. Nader, Ph.D. January/1984 - June/1987
Structure of trypanosomatids glycoproteins
Instituto de Investigaciones Bioquimicas "Fundacion Campomar", Buenos Aires - Argentina Research Fellow; Supervisor: Armando J. Parodi, Ph.D March/1986 - November/1986
Curriculum Vitae Carlos T. Moraes 2
Molecular basis of Mitochondrial Encephalomyopathies Department of Genetics & Development - Columbia University, New York - USA Ph.D. candidate and Postdoctoral fellow; Supervisors: Eric A. Schon Ph.D. and Salvatore
DiMauro MD. September/1987 - June/1993
Mitochondrial Function/Dysfunction
Department of Neurology - University of Miami School of Medicine Research Assistant Professor, June/1993 - June/1995 Assistant Professor June/1995 – May/1997 Associate Professor (with tenure) June/1997 – May/2003
Professor June 2003 - Present
15. Non-Academic: None 16. Military: None
PUBLICATIONS
17. Peer-reviewed book and special issue chapters:
1. Clinical and Biochemical studies on Cytochrome Oxidase deficiencies.
Schon, E.A., Bonilla, E., Lombes, A., Moraes, C.T., Nakase, H., Rizzuto, R., Zeviani, M. and DiMauro, S. Annals of New York Academy of Sciences, 550: 348-359. (1988)
2. Mitochondrial Encephalomyopathies DiMauro, S, Zeviani, M, Moraes,C.T., Nakase, H, Rizzuto, R, Lombes, A, Shanske, S, and
Schon, E.A. Genetics of Neuromuscular Disorders, pp. 117-128. Alan Liss, Inc. New York, NY (1989).
3. Mitochondrial Diseases
DiMauro, S., Lombes, A., Nakase, H., Zeviani, M., Rizzuto, R., Moraes,C.T., and Schon, E.A. Molecular Basis of Membrane-Associated Diseases. Azzi, A., Drahota, Z., and Papa, S. eds. pp 157-166. Springer-Verlag Press, Berlin, Heidelberg (1989).
4. Mitochondrial Encephalomyopathies
DiMauro, S., Bonilla, E., Lombes, A., Shanske, S., Minetti, C., Moraes, C.T. Neurological Clinics 8: 483-506 (1990).
5. Doenças Mitocondriais
Moraes, C.T., DiMauro, S., Schmidt, B., Schon, E.A. Ciencia Hoje (in Portuguese)12: 46-52 (1990)
6. Mitochondrial Diseases: Toward a Rational Classification
Moraes, C.T., Schon, E.A., & DiMauro, S. Current Neurology, vol 11. Appel, S.H. ed., Mosby Year Book. pp 83-120 (1991)
Curriculum Vitae Carlos T. Moraes 3
7. Mitochondrial Encephalomyopathies- A Biochemical Approach
DiMauro, S., Moraes, C.T., Shanske, S., Lombes, A., Nakase, H., Mita, S., Tritchler, H.J., Bonilla, E., Miranda, A.F., & Schon, E.A.
Reveu Neurologique (Paris) 147: 443-449 (1991)
8. Mitochondrial Encephalomyopathies: Problems of Classification
DiMauro, S., Moraes, C.T., & Schon, E.A. Progress in Neuropathology, vol. 7. Sato, T. & DiMauro, S. eds. Raven Press, Ltd., New York. pp 113-127 (1991)
9. Analysis of giant deletions of human mitochondrial DNA in Progressive External
Ophthalmoplegia Schon, E.A., Moraes, C.T., Mita, S., et al. Molecular Basis of Neurological Disorders and
their treatment. Gorrod J.W., Albano, O., Ferrari, E. & Papa, S. eds. Chapman and Hall ltd. London, pp 209-220 (1991).
10. Mitochondrial Myopathies
Schon, E.A., Tritchler, H.J., Moraes, C.T., Arnaudo, E., DiMauro, S., & Bonilla, E. International Pediatrics 7: 23-27 (1992)
11. New morphological approaches to the study of mitochondrial encephalomyopathies
Bonilla, E., Sciacco, M., Tanji, K., Sparaco, M., Petruzzella, V., & Moraes, C.T. Brain Pathology 2: 155-162 (1992)
12. Disorders associated with depletion of mitochondrial DNA
Ricci, E., Moraes, C.T., Servidei, S., Tonali, P., Bonilla, E., & DiMauro, S. Brain Pathology 2: 185-1192 (1992)
13. Mitochondrial encephalomyopathies
DiMauro, S. and Moraes, C.T. Arch. Neurol. 50: 1197-1207 (1993)
14. Quantitative defects of mitochondrial DNA
Moraes, C.T., Ricci, E., Arnaudo, E., Bonilla, E., DiMauro, S., Schon, E.A. Mitochondrial DNA in Human Pathology, DiMauro,S. and Wallace, D. eds. Raven Press,
Ltd., New York. pp. 97-108 (1993)
15. Mitochondrial dysfunction as a mechanism of CNS injury
DiMauro, S., Simonetti, S., Chen, X., Petruzzella, V., Hirano, M, Shanske, S., Moraes, C.T., Schon, E.A. Mollecular and Cellular Approaches to the treatment of Neurological Disease. Waxman, S.G. ed. Raven Press, Ltd., New York. pp 67-79 (1993)
16. Quantitative defects of mitochondrial DNA in human pathologies
Moraes, C.T. International Pediatrics 8:40-44 (1993)
17. Mitochondrial diseases: defects of mitochondrial DNA
Moraes, C.T., DiMauro, and Schon, E.A. New Trends in Pediatric Neurology. Fejerman, N. and Chamoles, N.A. eds. Elsevier Science, Amsterdan. pp 227-232 (1993)
18. Cytochrome oxidase deficiency: progress and problems
Curriculum Vitae Carlos T. Moraes 4 DiMauro, S., Hirano, M., Bonilla, E., Moraes, C.T., Schon, E.A. Mitochondrial Disorders in Neurology. Schapira, A.H.V. and DiMauro, S. eds. Butterworth-
Heinemann, Oxford. pp 91-115 (1994)
19. Phenotype-genotype correlations in skeletal muscle of patients with mtDNA deletions.
Moraes, C.T., Siacco, M., Ricci, E., Tengan, C.H., Hao, H., Bonilla, E., Schon, E.A., DiMauro, S. Muscle & Nerve, 18 supplement 3: S150-S153 (1995).
20. Replication of a heteroplasmic population of normal and partially-deleted human
mitochondrial genomes.
Moraes, C.T. and Schon, E.A. Progress in Cell Research: Symposium on "Thirty Years of Progress in Mitochondrial
Bioenergetics and Molecular Biology". F. Palmieri, S. Papa, C. Saccone, and M.N. Gadaleta, eds., Elsevier, 5: 209-215 (1995).
21. Phenotypical expression and molecular genetics of the nt-3243 mutation in the
mitochondrial tRNALEU(UUR) gene.
Petruzzella, V., Hirano, M., Moraes, C.T., DiMauro, S., Bonilla, E., and Schon, E.A. Bull. Mol. Biol. Med. 20:177-183 (1995).
22. In situ hybridization and single muscle fiber PCR in the study of mitochondrial
genotypes.
Moraes, C.T., and Schon, E.A. Methods in Enzymology. volume Editors: Giuseppe M. Attardi, and Anne Chomyn. (1996),
264:522-540.
23. Mitochondrial disorders
Carlos T. Moraes Current Opinion in Neurology, 9:369-374 (1996)
24. Deletions of mitochondrial DNA in Kearns-Sayre syndrome. 1988 [classical article].
Zeviani, M., Moraes, C. T., DiMauro, S., Nakase, H., Bonilla, E., Schon, E. A. and Rowland, L. P. Neurology, 51:1525 and 8 pages following (1998)
25. Cybrids in Alzheimer's disease: a cellular model of the disease? [letter; comment]. Schon, E. A., Shoubridge, E. A. and Moraes, C. T. Neurology, 51:326-7 (1998)
26. Mitochondrial DNA mutations in human diseases.
Carlos T. Moraes in: MITOCHONDRIAL DNA MUTATIONS IN AGING, DISEASE AND CANCER. Ed Singh, K.K. Chapman & Hall press (1998)
27. What regulates mitochondrial DNA copy number in animal cells?
Moraes, C. T. Trends Genet 17, 199-205. (2001)
28. Transmitochondrial Technology in Animal Cells
Carlos T. Moraes, Runu Dey, and Antoni Barrientos Methods in Cell Biology 65, 397-412 (2001)
Curriculum Vitae Carlos T. Moraes 5
29. Reactive Oxygen Species and mitochondrial diseases
Kirkinezos, I.G. and Moraes, C.T. Seminars in Cell & Developmental Biology 12, 449-457 (2001)
30. A Helicase is Born (News & Views)
Moraes, C.T. Nature Genetics 28:200-201. (2001)
31. What regulates mitochondrial DNA copy number in animal cells?
Moraes, C. T. Trends Genet 17, 199-205. (2001)
32. Studying Mitochondria of Animal Cells [Issue Editor and Editorial]
Moraes, C.T. Methods 26, 291 (2002)
33. Mitochondrial DNA structure and function
Moraes CT, Srivastava S, Kirkinezos I, Oca-Cossio J, van Waveren C, Woischnick M, Diaz F. Int Rev Neurobiol 53:3-23 (2002).
34. Techniques And Pitfalls In The Detection Of Pathogenic Mitochondrial DNA Mutations
Carlos T. Moraes, David P. Atencio, Jose Oca-Cossio And Francisca Diaz Journal of Molecular Diagnosis 5:197-208 (2003)
35. Book Review: Genetics of Mitochondrial Diseases. Edited by Ian J. Holt.
Carlos T. Moraes Am. J. Hum. Genet. 75:733, (2004)
36. Defects in the Biosynthesis of Mitochondrial Heme c and Heme a in Yeast and
Mammals Carlos T. Moraes, Franscisca Diaz and Antoni Barrientos BBA – Bioenergetics 1659:153-159 (2004)
37. Mitochondrial Involvement in Amyotrophic Lateral Sclerosis: Trigger or Target?
Sandra R. Bacman, Walter G. Bradley and Carlos T. Moraes* Molecular Neurobiology 33:113-131 (2006)
38. Transmitochondrial technology in animal cells.
Bacman SR, Moraes CT. Methods Cell Biol. 80:503-524 (2007)
39. Microdissection and Analytical PCR for the Investigation of mtDNA Lesions
Williams SL, Moraes CT. Methods Cell Biol. 80:481-501 (2007)
40. Mitochondrial Disorders
Verma, A., Hirano, M. and Moraes CT. Neurology in Clinical Practice (fifth edition). Bradley, Daroff, Fenichel and Jankovic (eds). Elsevier Press. Volume II:1785-1798 (2008)
41. Mitochondrial biogenesis and turnover.
Curriculum Vitae Carlos T. Moraes 6 Diaz F, Moraes CT. Cell Calcium. 44:24-35 (2008)
42. The mitochondrial impairment, oxidative stress and neurodegeneration connection:
reality or just an attractive hypothesis?
Hirokazu Fukui and Carlos T. Moraes Trends in Neurosciences 31: 251-256 (2008)
43. Mouse models of oxidative phosphorylation dysfunction and disease
Uma D. Vempati, Alessandra Torraco and Carlos T. Moraes Methods 46: 241–247 (2008)
44. Mouse models of oxidative phosphorylation defects: Powerful tools to study the
pathobiology of mitochondrial diseases
Alessandra Torraco, Francisca Diaz, Uma D. Vempati and Carlos T. Moraes Biochem. Biophys. Acta 1793: 171–180 (2009)
45. Making the most of what you’ve got: Optimizing residual OXPHOS function in
mitochondrial diseases Carlos T. Moraes EMBO Mol. Med. 1:357-359 (2009)
46. The role of cytochrome c oxidase deficiency in ROS and amyloid plaque formation.
Pickrell AM, Fukui H, Moraes CT. J Bioenerg Biomembr. 41:453-456 (2009).
47. Emerging therapeutic approaches to mitochondrial diseases.
Wenz T, Williams SL, Bacman SR, Moraes CT. Dev Disabil Res Rev. 16:219-29 (2010).
48. What role does mitochondrial stress play in neurodegenerative diseases?
Pickrell AM, Moraes CT. Methods Mol Biol. 648:63-78 (2010).
49. Mitochondrial disorders caused by mutations in respiratory chain assembly factors.
Diaz F, Kotarsky H, Fellman V, Moraes CT. Semin Fetal Neonatal Med.16:197-204 (2011)
50. Mitochondrial DNA transcription regulation and nucleoid organization.
Rebelo AP, Dillon LM, Moraes CT. J Inherit Metab Dis. 34:941-51 (2011)
51. Mitochondrial transcription: Lessons from mouse models.
Susana Peralta, Xiao Wang and Carlos T. Moraes Biochem. Biophys. Acta 1819:961-9 (2012)
52. Regional susceptibilities to mitochondrial dysfunctions in the CNS
Milena Pinto, Alicia M. Pickrell, and Carlos T. Moraes Biolog. Chem. 393: 275–281 (2012)
Curriculum Vitae Carlos T. Moraes 7
53. The role of PGC-1 coactivators in aging skeletal muscle and heart.
Dillon LM, Rebelo AP, Moraes CT. IUBMB Life. 64:231-41 (2012).
54. Mouse models of Parkinson's disease associated with mitochondrial dysfunction.
Pickrell AM, Pinto M, Moraes CT. Mol Cell Neurosci. 55:87-94 (2013).
55. Mitochondrial Alterations During Carcinogenesis
Xiao Wand, Susana Peralta and Carlos T. Moraes Advances in Cancer Research, 119:127-60 (2013).
56.Adrenoleukodystrophy and the mitochondrial connection: Clues for supplementing
Lorenzo's oil.
Carlos T. Moraes Brain, 136:2339-41 (2013).
57. Translational research in primary mitochondrial diseases: Challenges and
opportunities.
Moraes CT, Anderson V, Mohan C; Workshop participants. Mitochondrion. Aug 17. doi:pii: S1567-7249 (2013)
58. Mitochondrial genome changes and neurodegenerative diseases.
Pinto M, Moraes CT. Biochim Biophys Acta. S0925-4439(13)00337-2. (2013)
59. Manipulating mitochondrial genomes in the clinic: playing by different rules.
Moraes CT, Bacman SR, Williams SL. Trends Cell Biol.;24:209-11. (2014)
60. A magic bullet to specifically eliminate mutated mitochondrial genomes from patients'
cells.
Moraes CT. EMBO Mol Med.;6:434-5. (2014)
61. The Use of Mitochondria-Targeted Endonucleases to Manipulate mtDNA
Sandra R. Bacman, Sion L. Williams, Milena Pinto, Carlos T. Moraes Methods in Enzymology, 547:373-97 (2014)
62. Mechanisms linking mtDNA damage and aging
Milena Pinto, Carlos T. Moraes Free Rad. Biol. Med., 85:250-8 (2015)
63. Current strategies towards therapeutic manipulation of mtDNA heteroplasmy
Pereira CV, Moraes CT. Front Biosci (Landmark Ed) 22:991-1010 (2017).
Curriculum Vitae Carlos T. Moraes 8
64. NO control of mitochondrial function in normal and transformed cells.
Tengan CH, Moraes CT. Biochim Biophys Acta. 1858(8):573-581 (2017).
65. Mitochondrial genome engineering: The revolution may not be CRISPR-ized.
Payam A Gammage, Carlos T. Moraes and Michal Minczuk Trends in Genetics. S0168-9525(17)30191-9. (2017).
66. Mitochondrial DNA damage and reactive oxygen species in neurodegenerative disease.
Nissanka N, Moraes CT. FEBS Lett. 592(5):728-742. (2018).
67. Targeted mitochondrial genome elimination
Bacman SR., Pereira CV, Moraes CT. Mitochondrial Biology and Experimental Therapeutics. PJ Oliveira (ed) Springer Publishing. 535-563 (2018).
18. Juried or refereed original journal articles:
*Corresponding author
1. Isolation and characterization of a heparin with high anticoagulant activity from
Anomalocardia brasiliana.
Dietrich, C.P., Paiva, J.F., Moraes, C.T., Takahashi, H.K., Porcionatto, M.A. & Nader, H.B. Biochimica et Biophysica Acta , 843:1-7 (1985)
2. Structural characterization of several galactofuranose-containing, high mannose type
oligosaccharides present in glycoproteins of the trypanosomatid Leptomonas samueli.
Moraes, C.T., Bosh, M. & Parodi, A.J. Biochemistry, 27: 1543-1549 (1988)
3. Mitochondrial DNA deletions in Kearns-Sayre syndrome
Zeviani, M., Moraes, C.T., DiMauro, S., Nakase, H., Bonilla, E., Schon, E. & Rowland, L.P. Neurology, 38: 1399-1346 (1988)
4. Immunocytochemical study of dystrophin in muscle cultures from patients with
Duchenne muscular dystrophy and unaffected controls. Miranda, A.J., Bonilla, E., Martucci, G., Moraes, C.T., Hays, A.P., and DiMauro, S.
American Journal of Pathology, 132: 410-416. (1988)
5. Heteroplasmy of mitochondrial genomes in clonal cultures from patients with Kearns-
Sayre syndrome.
Moraes, C.T., Schon, E.A., DiMauro, S & Miranda, A.F. Biochemical and Biophysical Research Communications, 160: 765-71 (1989).
6. Mitochondrial DNA deletions in progressive external ophthalmoplegia and Kearns-
Sayre syndrome.
Curriculum Vitae Carlos T. Moraes 9 Moraes, C.T., DiMauro, S., Zeviani, M., Lombes, A., Shanske, S., Miranda, A. F., Nakase,
H., Bonilla, E., Wernec, L. C., Servidei, S., Nonaka, I., Koga, Y., Spiro, A., Brownell, K. W., Schmidt, B., Schotland, D. L., Zupanc, M. D., DeVivo, D. C., Schon E. A. and Rowland, L. P. New England Journal of Medicine, 320: 1293-1299 (1989).
7. A direct repeat is a "hot spot" for mitochondrial DNA deletions in humans.
Schon, E.A., Rizzuto, R., Moraes, C.T., Nakase, H., Zeviani, M. & DiMauro, S. Science, 244: 346-349 (1989).
8. Recombination via flanking direct repeats is a major cause of large-scale deletions of
human mitochondrial DNA.
Mita, S, Rizzuto, R, Moraes, C.T., Shanske, S., Arnaudo, E., Fabrizi, G.M., Koga, Y., DiMauro, S. and Schon, E.A. Nucleic Acids Research, 18: 561-567 (1990)
9. Widespread tissue distribution of mitochondrial DNA deletions in Kearns-Sayre
syndrome.
Shanske, S., Moraes, C.T., Lombes, A., Miranda, A.F., Bonilla, E., Lewis, P., Whealan, M.A., Ellsworth, C.A., DiMauro, S. Neurology, 40: 24-28 (1990)
10. Transcription and translation of deleted mitochondrial genomes in Kearns-Sayre
syndrome: Implications for pathogenesis.
Nakase, H., Moraes, C.T., Rizzuto, R., Lombes, A., DiMauro, S. and Schon, E.A. American Journal of Human Genetics, 46: 418-427 (1990).
11. Purification and substrate specificity of heparitinase I and heparitinase II from
Flavobacterium heparinum.
Nader, H.B., Porcionatto, M.A., Tersariol, I.L.S., Pinhal, M.A.S., Oliveira, F.W., Moraes, C.T. & Dietrich, C.P. The Journal of Biological Chemistry, 265: 16807-16813 (1990)
12. Mitochondrial DNA depletion with variable tissue expression: A novel genetic
abnormality in mitochondrial diseases.
Moraes, C.T., Shanske, S., Trishler H-J., Aprille, J.R., Bonilla, E., Schon, E.A. and DiMauro, S. American Journal of Human Genetics, 48: 492-501 (1991).
13. Depletion of mitochondrial DNA in AIDS patients with zidovudine-induced myopathy.
Arnaudo, E., Dalakas, M., Shanske, S., Moraes, C.T., DiMauro, S. and Schon, E.A. The Lancet, 337: 508-510 (1991)
14. Replication-competent human mitochondrial DNA lacking the heavy-strand promoter
region.
Moraes, C.T., Andreetta, F., Bonilla, E., Shanske, S., DiMauro, S., and Schon, E.A. Molecular and Cellular Biology, 11: 1631-1637 (1991)
15. Mitochondrial DNA deletions in a girl with manifestations of Kearns-Sayre and Lowe
syndromes: An example of phenotypic mimicry?
Moraes, C.T., Zeviani, M., Schon, E.A., Hickman, R.O., Vlcek, B.W., & DiMauro, S. American Journal of Medical Genetics, 41:301-305 (1991)
Curriculum Vitae Carlos T. Moraes 10
16. Deletions of mitochondrial DNA in patients with combined features of Kearns-Sayre
and MELAS syndromes.
Zupanc, M.L., Moraes, C.T., Shanske, S., Langman, C.B., Ciafaloni, E., & DiMauro, S. Annals of Neurology, 29: 680-683 (1991)
17. Mitochondrial myopathy of childhood associated with depletion of mitochondrial DNA.
Tritschler, H.-J., Andreetta, F., Moraes, C.T., Bonilla, E., Arnaudo, E., Danon, M.J., Glass, S., Zelaya, B.M., Vamus, E., Telerman-Toppet, N., Shanske, S., Kadembach, B., DiMauro, S., & Schon, E.A. Neurology, 42: 209-217 (1992)
18. The mitochondrial tRNALeu(UUR) mutation in mitochondrial encephalopathy, lactic
acidosis, and strokelike episodes (MELAS): genetic, biochemical, and morphological
correlations in skeletal muscle.
Moraes, C.T., Ricci, E., Bonilla, E., DiMauro, S. & Schon, E. A. American Journal of Human Genetics, 50: 934-949 (1992).
19. MELAS: clinical features, biochemistry, and molecular genetics.
Ciafaloni, E., Ricci, E., Shanske, S., Moraes, C. T., Silvestri, G., Hirano, M., Simonetti, S., Angelini, C., Donati, A., Garcia, C., Martinuzzi, A., Mosewich, R., Servidei, S., Zammarchi, E., Bonilla, E., DeVivo, D. C., Rowland, L. P., Schon, E. A. and DiMauro, S.
Annals of Neurology 31: 391-398 (1992).
20. Molecular analysis of the muscle pathology associated with mitochondrial DNA
deletions.
Moraes, C. T., Ricci, E., Petruzzella, V., Shanske, S., DiMauro, S., Schon, E.A. and Bonilla, E. Nature Genetics, 1: 359-367 (1992).
21. Fatal infantile liver failure associated with mitochondrial DNA depletion.
Mazziotta, M.R.M., Ricci, E., Bertini, E., Dionisi-Vici, C., Servidei, S., Burlina, A.B., Sabetta, G., Bartuli, A., Manfredi, G., Silvestri, G., Moraes, C.T., DiMauro, S.
Journal of Pediatrics, 121:896-901 (1992).
22. A new mtDNA mutation in the tRNALys gene associated with myoclonic epilepsy and
ragged-red fibers (MERRF).
Silvestri, G., Moraes, C.T., Shanske, S., Oh, S.J., DiMauro, S. American Journal of Human Genetics, 51:1213-1217 (1992).
23. Structural and functional abnormalities associated with high levels of partially deleted
mtDNA in somatic cell hybrids.
Sancho, S., Moraes, C.T., Tanji, K., and Miranda, A.F. Somatic Cell and Molecular Genetics, 18:431-442 (1992).
24. The mitochondrial tRNALeu(UUR) mutation in MELAS: a model for pathogenesis.
Schon, E.A., Koga, Y., Davidson, M., Moraes, C.T., King, M.P. Biochimica et Biophysica Acta, 1101:206-209 (1992).
25. A mitochondrial tRNA anticodon swap associated with a muscle disease.
Moraes, C.T., Ciacci, F., Bonilla, E., Ionascescu, V., Schon, E.A., and DiMauro, S.
Curriculum Vitae Carlos T. Moraes 11 Nature Genetics, 4:284-287 (1993).
26. Atypical clinical presentations associated with the MELAS mutation at position 3243 of
human mitochondrial DNA.
Moraes, C.T., Ciacci, F., Silvestri, G., Shanske, S., Sciacco, M., Hirano, M., Schon, E.A., Bonilla, E., and DiMauro, S.
Neuromuscular Disorders, 3:43-50 (1993).
27. Two novel pathogenic mitochondrial DNA mutations affecting organelle number and
protein synthesis: is the tRNALeu(UUR) gene an etiologic hot spot ?
Moraes, C.T.*, Ciacci, F., Bonilla, E., Jansen, C., Hirano, M., Rao, N., Lovelace, R.E., Rowland, L.P., Schon, E.A., and DiMauro, S.
Journal of Clinical Investigations, 92:2906-2915 (1993).
28. Sequencing of heparan sulfate proteoglycans: identification of variable and constant
oligosaccharide regions in eight heparan sulfate proteoglycans of different origins.
Tersariol, I., Ferreira, T.M., Medeiros, M.G., Porcionatto, M.A., Moraes, C.T., Abreu, L.R.D., Nader, H.B., and Dietrich, C.P.
Brazilian Journal of Medical and Biological Research 27:2097-2102 (1994).
29. Clinical and morphological features of a myopathy associated with a mutation in the
mitochondrial tRNAPro gene. Ionasescu, V.V., Hart, M., DiMauro, S., Moraes, C.T.* Neurology, 44:975-977 (1994)
30. Distribution of wild-type and common deletion forms of mtDNA in normal and
respiration-deficient muscle fibers from patients with mitochondrial myopathy.
Siacco, M., Bonilla, E., Schon, E.A., DiMauro, S., and Moraes, C.T.* Human Molecular Genetics, 3:13-19 (1994).
31. Extremely high levels of mutant mtDNAs co-localize with cytochrome c oxidase-negative
ragged-red fibers in patients harboring a point mutation at nt 3243.
Petruzzella, V., Moraes, C.T., Bonilla, E., DiMauro, S., and Schon, E.A. Human Molecular Genetics, 3:449-454 (1994)
32. Segregation patterns of a novel mutation in the mitochondrial tRNA glutamic acid gene
associated with myopathy and diabetes mellitus.
Hao, H., Bonilla, E., Manfredi, G., DiMauro, S., Moraes, C.T.* American Journal of Human Genetics, 56:1017-1025 (1995).
33. High levels of mitochondrial DNA with an unstable 260 base pair duplication in a
patient with a mitochondrial myopathy
Manfredi, G., Servidei, S., Bonilla, E., Shanke,S, Schon, E., DiMauro, S., and Moraes, C.T.* Neurology, 45:762-768 (1995)
34. A new mutation associated with MELAS is located in a mitochondrial DNA polypetide-
coding gene.
Manfredi, G., Schon, E.A., Moraes, C.T., Bonilla, E., DiMauro, S.
Curriculum Vitae Carlos T. Moraes 12 Neuromuscular Disorders, 5:391-398 (1995)
35. Identification of a mutation in the mitochondrial tRNACys gene associated with
mitochondrial encephalo-myopathy.
Manfredi, G., Schon, E.A., Bonilla, E., Moraes, C.T., Shanske, S., DiMauro, S. Human Mutation, 7:158-163 (1996)
36. Functional and Molecular Mitochondrial Abnormalities Associated with a CT
Transition at Position 3256 of the Human Mitochondrial Genome
Hao, H. and Moraes, C.T.* The Journal of Biological Chemistry 271:2347-2352 (1996).
37. A MERRF/PEO overlap syndrome associated with the mitochondrial DNA 3243
mutation
Ashok Verma, Carlos T. Moraes, Robert T. Shebert, Walter G. Bradley. Neurology 46:1334-1336 (1996)
38. Detection and analysis of mitochondrial DNA deletions by whole genome PCR
Celia H. Tengan and Carlos T. Moraes* Biochemical and Molecular Medicine 58:130-134 (1996)
39. Functional and structural features of a tandem duplication of the human mitochondrial
DNA promoter region Hao, H., Manfredi, G., Moraes, C. T.* American Journal of Human Genetics 60:1363-1372 (1997)
40. Upregulation of D3 dopamine receptor mRNA in the nucleus accumbens of human
cocaine fatalities Segal, D., Moraes, C.T., Mash, D.C. Molecular Brain Research 45:335-339 (1997)
41. Expanding the functional human mitochondrial DNA database by the establishment of
primate xenomitochondrial cybrids
Lesley Kenyon and Carlos T. Moraes* Proceedings of the National Academy of Sciences USA 94:9131-9135 (1997)
42. A Novel Mitochondrial G8313A Mutation Associated with Prominent Initial
Gastrointestinal Symptoms and Progressive Encephaloneuropathy
Ashok Verma, David A. Piccoli, Eduardo Bonilla, Gerald T. Berry, Salvatore DiMauro, and
Carlos T. Moraes*
Pediatric Research 42:448-454 (1997)
43. Oxidative phosphorylation dysfunction does not increase the rate of accumulation of
age-related mtDNA deletions in skeletal muscle
C.H. Tengan, A.A. Gabbai, S. Shanske, M. Zeviani, C.T. Moraes* Mutation Research 379:1-11 (1997)
Curriculum Vitae Carlos T. Moraes 13
44. A Disease-Associated G5703A Mutation in the Human Mitochondrial DNA Causes a
Conformational Change and a Marked Decrease in Steady-State Levels of the
Mitochondrial tRNAAsn
H. Hao and C.T. Moraes* Molecular and Cellular Biology 17:6831-6837 (1997)
45. Mitochondrial DNA defects in Brazilian patients with chronic progressive external
ophtalmoplegia
B.H. Kiyomoto, C.H. Tengan, C.T. Moraes, A.S.B. Oliveira, A.A. Gabbai Journal of Neurological Science 152:160-165 (1997)
46. Mitochondrial Encephalomyopathy and Hypoparathyrodism Associated with a
Duplication and a Deletion of Mitochondrial DNA
C.H. Tengan, B.H. Kiyomoto, M.S. Rocha, V.L.S. Tavares, A.A. Gabbai, C.T. Moraes*
Journal of Clinical Endocrinology & Metabolism 83:125-129 (1998)
47. Simultaneous transfer of mitochondrial DNA and single chromosomes in somatic cells:
a novel approach for the study of defects in nuclear-mitochondrial communication. Barrientos, A. and Moraes, C. T.* Hum Mol Genet, 7:1801-8 (1998)
48. Human xenomitochondrial cybrids. Cellular models of mitochondrial complex I
deficiency. Barrientos, A., Kenyon, L. and Moraes, C. T.* J Biol Chem, 273:14210-7 (1998)
49. Structure of heparan sulfate: identification of variable and constant oligosaccharide
domains in eight heparan sulfates of different origins. Dietrich, C. P., Tersariol, I. L., Toma, L., Moraes, C. T., Porcionatto, M. A., Oliveira, F. W. and Nader, H. B. Cell Mol Biol (Noisy-le-grand), 44:417-29 (1998)
50. Duplication and triplication with staggered breakpoints in human mitochondrial DNA.
Tengan, C. H. and Moraes, C. T.* Biochim Biophys Acta, 1406:73-80 (1998)
51. Dopamine transporter mRNA in autopsy studies of chronic cocaine users. Chen, L., Segal, D. M., Moraes, C. T. and Mash, D. C. Brain Res Mol Brain Res, 73:181-185 (1999)
52. Sequence analysis of the diabetes-protective human leukocyte antigen-DQB1*0602 allele
in unaffected, islet cell antibody-positive first degree relatives and in rare patients with
type 1 diabetes. Pugliese, A., Kawasaki, E., Zeller, M., Yu, L., Babu, S., Solimena, M., Moraes, C. T., Pietropaolo, M., Friday, R. P., Trucco, M., Ricordi, C., Allen, M., Noble, J. A., Erlich, H. A. and Eisenbarth, G. S. J Clin Endocrinol Metab, 84:1722-8 (1999)
53. Mechanisms of human mitochondrial DNA maintenance: the determining role of
primary sequence and length over function. Moraes, C. T.*, Kenyon, L. and Hao, H. Mol Biol Cell, 10:3345-56 (1999)
Curriculum Vitae Carlos T. Moraes 14
54. Titrating the effects of mitochondrial complex I impairment in the cell physiology. Barrientos, A. and Moraes, C. T.* J Biol Chem, 274:16188-97 (1999)
55. Suppression of a mitochondrial tRNA gene mutation phenotype associated with changes
in the nuclear background. Hao, H., Morrison, L. E. and Moraes, C. T.* Hum Mol Genet, 8:1117-24 (1999)
56. Lack of Oxidative Phosphorylation and Low Mitochondrial Membrane Potential
Decrease Susceptibility to Apoptosis and Do not Modulate the Protective Effect of Bcl-
xL in Osteosarcoma Cells.
Dey, R. and Moraes, C.T.*. J. Biol. Chem. 275:7087-7094 (2000)
57. Mitochondrial function in heart muscle from patients with idiopathic dilated
cardiomyopathy. Jarreta D., Orus J., Barrientos A., Miro O, Roig E., Heras M., Moraes C.T., Cardellach F., Casademont J. Cardiovasc Res. 45:860-5 (2000).
58. A Novel Myopathy-Associated mtDNA Mutation Altering the Conserved Size of the
tRNAGln Anticodon Loop.
Runu Dey, Celia H. Tengan, Maria P.A. Morita, Beatriz H. Kiyomoto and Carlos T. Moraes*
Neuromuscular Disorders 10:488-492 (2000).
59. Functional Constraints of Nuclear - Mitochondrial DNA Interactions in Xeno-
mitochondrial Rodent Cell Lines
Runu Dey, Antoni Barrientos, and Carlos T. Moraes* J Biol Chem, 275: 31520-31527 (2000).
60. Cloning of an Endangered Species (Bos gaurus) Using Interspecies Nuclear Transfer
Robert P. Lanza, Jose B. Cibelli, Francisca Diaz, Carlos T. Moraes, Peter W. Farin, Charlotte E. Farin, Carolyn J. Hammer, Michael D. West & Philip Damiani Cloning, 2:79-88 (2000).
61.° Tumor Cells: A model for studying whether mitochondria are targets for rhodamine
123, doxorubicin, and other drugs.
Yan-Ping Hu, Carlos T. Moraes, Niramol Savaraj, Waldemar Priebe and Theodore J. Lampidis. Biochemical Pharmacology, 60:1897-1905. (2000).
62. Cytochrome c Oxidase Assembly in Primates is Sensitive to Small Evolutionary
Variations in Amino Acid Sequence
Antoni Barrientos, Stefan Müller, Runu Dey, Johannes Wienberg and Carlos T. Moraes* Molecular Biology and Evolution, 17: 1508-1519 (2000)
63. An out of Frame Cytochrome b Gene Deletion from a Patient with Parkinsonism is
Associated with Impaired Complex III Assembly and Increase in Free Radicals
Production
Michele Rana, Irenaeus de Coo, Francisca Diaz, Hubert Smeets, and Carlos T. Moraes* Annals of Neurology;48:774-781 (2000).
64. Dysfunctional Mitochondrial Respiration in the Wobbler Mouse Brain
Curriculum Vitae Carlos T. Moraes 15
Guang-Ping Xu, Kunjan R. Dave, Carlos T. Moraes, Raul Busto, Thomas J. Sick, Walter G. Bradley and Miguel A. Pérez-Pinzón Neuroscience Letters, 300:141-144. (2001)
65. Manipulating mitochondrial DNA heteroplasmy by a mitochondrially targeted
restriction endonuclease Srivastava, S. and Moraes, C.T.* Hum Mol Genet 10: 3093-3099 (2001)
66. Pattern of organization of human mitochondrial pseudogenes in the nuclear genome.
Woischnik M, Moraes CT.* Genome Research 12:885-93. (2002)
67. Generation of histocompatible tissues using nuclear transplantation.
Lanza RP, Chung HY, Yoo JJ, Wettstein PJ, Blackwell C, Borson N, Hofmeister E, Schuch G, Soker S, Moraes CT, West MD, Atala A Nat Biotechnol 20:689-96 (2002)
68. Human mitrochondrial DNA with large deletions repopulates organelles faster than
full-length genomes under relaxed copy number control.
Francisca Diaz, Maria Pilar Bayona-Bafaluy, Michele Rana, Marialejandra Mora, Huiling Hao and Carlos T. Moraes* Nucleic Acids Research, 30:300-310 (2002)
69. Mitochondrial DNA depletion: mutations in thymidine kinase gene with myopathy and
SMA.
Mancuso M, Salviati L, Sacconi S, Otaegui D, Camano P, Marina A, Bacman S, Moraes CT, Carlo JR, Garcia M, Garcia-Alvarez M, Monzon L, Naini AB, Hirano M, Bonilla E, Taratuto AL, DiMauro S, Vu TH. Neurology, 59:1197-202 (2002).
70. Bcl-2 Modulates Adenine Nucleotides influx into Mitochondria and improves Oxidative
Phosphorylation Dysfunction Caused by Mitochondrial tRNA Gene Mutations
Giovanni Manfredi, Jennifer Q. Kwong, Jose A. Oca-Cossio, Markus Woischenick, Katherine Martushova, Marilena D’Aurelio, Carl D. Gajewsky, Carlos T. Moraes
J. Biol. Chem;278:5639-5645 (2003).
71. Regular Exercise is Beneficial to a Mouse Model of Amyotrophic Lateral Sclerosis
Ilias G. Kirkinezos, Dayami Hernandez, Walter G. Bradley and Carlos T. Moraes* Ann. Neurol, 53: 804-807 (2003).
72. Decreased Mitochondrial tRNALys Steady-State Levels and Aminoacylation are
Associated with the Pathogenic G8313A Mitochondrial DNA Mutation
Sandra R. Bacman, David P. Atencio and Carlos T. Moraes* Biochem. J. 374:131-136 (2003).
73. Limitations of Allotopic Expression of Mitochondrial Genes in Mammalian Cells
Jose Oca-Cossio, Lesley Kenyon, Huiling Hao and Carlos T. Moraes*
Genetics165:100-114 (2003)
Curriculum Vitae Carlos T. Moraes 16
74. A Chemical Enucleation Method for the Transfer of Mitochondrial DNA to ° Cells.
Maria Pilar Bayona-Bafaluy, Giovanni Manfredi and Carlos T. Moraes* Nucleic Acids Research. 31, e98:4626-4633 (2003)
75. An ALS Mouse Model with a Permeable Blood-Brain Barrier Benefits from Systemic
Cyclosporine A Treatment.
Ilias G. Kirkinezos, Dayami Hernandez, Walter G. Bradley and Carlos T. Moraes* J. Neurochem. 88: 821-826 (2004)
76. Respiratory complex III is required to maintain complex I in mammalian mitochondria
Rebeca Acín-Pérez, María Pilar Bayona-Bafaluy, Patricio Fernández-Silva, Raquel Moreno-Loshuertos, Acisclo Pérez-Martos, Claudio Bruno, Carlos T. Moraes and José A. Enríquez Molecular Cell 13:805-815 (2004)
77. Erythromycin as a Potential Precipitating Agent in the Onset of Leber’s Hereditary
Optic Neuropathy
Corneliu C. Luca,., Byron L. Lam. and Carlos T. Moraes* Mitochondrion 4:31-36 (2004)
78. Heterologous mitochondrial DNA recombination in human cells.
D'Aurelio M, Gajewski CD, Lin M, Mauck W, Shao L, Lenaz G, Moraes CT, Manfredi G.
Hum Mol Genet. 13:3171-3179 (2004)
79. Cytochrome c Association with the Mitochondrial Inner Membrane is Impaired in the
Central Nervous System of G93A-SOD1 Mice Ilias G. Kirkinezos, Sandra R. Bacman, Dayami Hernandez, Jose Oca-Cossio, Laura J. Arias, Miguel A. Perez-Pinzon, Walter G. Bradley, and Carlos T. Moraes*. J. Neurosc. 25:164 –172 (2005)
80. Aberrant PKC Activation in Spinal Cord of Wobbler Mouse: A Model of Motor
Neuron Disease
Kunjan R. Dave, Ami P. Raval, Jesús Purroy, Ilias G. Kirkinezos, Carlos T. Moraes, Walter G. Bradley and Miguel A. Pérez-Pinzón
Neurobiology of Disease 18:126-33 (2005)
81. Fast Adaptive Co-evolution of Nuclear and Mitochondrial Subunits of ATP Synthetase
in Orangutan
Maria Pilar Bayona-Bafaluy, Stefan Müller, Carlos T. Moraes* Mol. Biol. Evol. 22:716-724 (2005)
82. Double-Strand Breaks of Mouse Muscle mtDNA Promote Large-Deletions Similar to
mtDNA Multiple Deletions in Humans
Sarika Srivastava and Carlos T. Moraes* Hum. Mol. Genet. 14:893-902 (2005)
83. Mice lacking COX10 in skeletal muscle recapitulate the phenotype of progressive
mitochondrial myopathies associated with cytochrome c oxidase deficiency Francisca Diaz, Christine K. Thomas, Sofia Garcia, Dayami Hernandez
Curriculum Vitae Carlos T. Moraes 17
and Carlos T. Moraes* Hum. Mol. Genet. 14: 2737–2748 (2005)
84. Rapid Directional Shift of Mitochondrial DNA Heteroplasmy in Animal Tissues by a
Mitochondrially-Targeted Restriction Endonuclease Maria Pilar Bayona-Bafaluy, Bas Blits, Brendan Battersby, Eric A. Shoubridge, and Carlos T. Moraes*. Proc. Natl. Acad. Sci. USA 102: 14392–14397 (2005)
85. Oxidative phosphorylation dysfunction modulates expression of extracellular matrix-
remodeling genes and invasion
Corina van Waveren, Yubo Sun, Herman S. Cheung, and Carlos T. Moraes* Carcinogenesis 27:409-418 (2006)
86. Cytochrome c Oxidase is Required for the Assembly/Stability of Respiratory Complex I
in Mouse Fibroblasts
Francisca Diaz, Hirokazu Fukui, Sofia Garcia and Carlos T. Moraes* Molecular and Cellular Biology 26:4872-4881 (2006)
87. Superoxide released into the mitochondrial matrix Danni Li, Bobby G. Poe, Marian Navratil, Carlos T. Moraes and Edgar A. Arriaga Free Radical Biology and Medicine 41:950-959 (2006)
88. Loss of Mcl-1 protein and inhibition of electron transport chain together induce anoxic
cell death. Brunelle JK, Shroff EH, Perlman H, Strasser A, Moraes CT, Flavell RA, Danial NN, Keith B, Thompson CB, Chandel NS. Mol Cell Biol. 27:1222-35 (2007)
89. The Role of Cytochrome c in Apoptosis: Increased Sensitivity to TNF-α is Associated
with Respiratory Defects But Not with Lack of Cytochrome c Release.
Uma D. Vempati, Francisca Diaz, Antoni Barrientos, Sonoko Narisawa, Abdul M. Mian, José Luis Millán, Lawrence H. Boise and Carlos T. Moraes* Mol Cell Biol. 27:1771-1783 (2007)
90. Extended polyglutamine repeats trigger a feedback loop involving the mitochondrial
complex III, the proteasome, and huntingtin aggregates.
Hirokazu Fukui1 and Carlos T. Moraes* Human Molecular Genetics16:783-797 (2007)
91. PGC-1α/β Upregulation is Associated with Improved Oxidative Phosphorylation in
Cells Harboring Nonsense mtDNA Mutations
Sarika Srivastava, John N. Barrett, and Carlos T. Moraes* Human Molecular Genetics 16:993-1005 (2007)
92. The Qo site of the mitochondrial complex III is required for the transduction of
hypoxic signaling via ROS production. Eric L. Bell, Tatyana A. Klimova, James Eisenbart, Carlos T. Moraes, Michael Murphy, G.R. Scott Budinger and Navdeep S. Chandel
Curriculum Vitae Carlos T. Moraes 18
J. Cell Biol. 177:1029-1036 (2007)
93. Modulating mtDNA heteroplasmy by mitochondria-targeted restriction endonucleases
in a “differential multiple cleavage-site” model.
Sandra R. Bacman, Sion L. Williams, Dayami Hernandez1 and Carlos T. Moraes* Gene Therapy, 14:1309-1318 (2007)
94. Cytochrome c Oxidase Deficiency in Neurons Decreases both Oxidative Stress and
Amyloid Formation in a Mouse Model of Alzheimer’s Disease. Hirokazu Fukui, Francisca Diaz, Sofia Garcia, Carlos T. Moraes* Proc Natl. Acad. Sci. USA 104 :14163–14168 (2007)
95. Systematic Association Studies of Mitochondrial DNA Variations in Schizophrenia:
Focus on the ND5 Gene. Bamne MN, Talkowski ME, Moraes CT, Manuck SB, Ferrell RE, Chowdari KV,
Nimgaonkar VL. Schizophr Bull. 34: :458-465 (2008)
96. Pathophysiology and Fate of Hepatocytes in a Mouse Model of Mitochondrial
Hepatopathies. Francisca Diaz, Sofia Garcia, Dayami Hernandez, Arie Regev, Adriana Rebelo, Jose Oca-
Cossio and Carlos T. Moraes* GUT 57:232-242 (2008)
97. Transcriptional Co-expression and Co-regulation of Genes Coding for Components of
the Oxidative Phosphorylation System
Corina van Waveren and Carlos T. Moraes* BMC Genomics, 9:18 (2008)
98. A 3’ UTR Modification of the Mitochondrial Rieske Iron Sulfur Protein in Mice Causes
a Skin Pigmentation Phenotype
Sofia Garcia, Francisca Diaz and Carlos T. Moraes* J. Invest. Dermat. 128:2343-5 (2008)
99. Mechanisms of formation and accumulation of mitochondrial DNA deletions in aging
neurons
Hirokazu Fukui and Carlos T. Moraes* Human Molecular Genetics 18:1028-36 (2009)
100. Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory
complexes I and IV
Uma D. Vempati, Xianlin Han, Carlos T. Moraes* Journal of Biological Chemistry 284:4383-91 (2009)
101. PGC-1α/β induced expression partially compensates for respiratory chain defects in
cells from patients with mitochondrial disorders
Sarika Srivastava, Francisca Diaz, Luisa Iommarini, Karine Aure, Anne Lombes and Carlos T. Moraes*
Curriculum Vitae Carlos T. Moraes 19
Human Molecular Genetics 18:1805-12. (2009)
102. Intra- and inter-molecular recombination of mitochondrial DNA after in vivo
induction of multiple double-strand breaks Sandra R. Bacman, Sion L. Williams and Carlos T. Moraes* Nucl. Acid Res. 37:4218-4226 (2009)
103. In vivo methylation of mtDNA reveals the dynamics of protein-mtDNA interactions
Adriana Rebelo, Sion L. Williams and Carlos T. Moraes* Nucl. Acid Res. 37:6701-6715 (2009)
104. Organ-specific shifts in mtDNA heteroplasmy following systemic delivery of a
mitochondria-targeted restriction endonuclease. Bacman SR, Williams SL, Garcia S, Moraes CT*.
Gene Ther. 17:713-20. (2010)
105. Mitochondrial myopathy induces a starvation-like response.
Tyynismaa H, Carroll CJ, Raimundo N, Ahola-Erkkilä S, Wenz T, Ruhanen H, Guse K, Hemminki A, Peltola-Mjøsund KE, Tulkki V, Oresic M, Moraes CT, Pietiläinen K, Hovatta I, Suomalainen A.
Hum Mol Genet. 19:3948-58 (2010).
106. The mtDNA mutation spectrum of the progeroid Polg mutator mouse includes
abundant control region multimers.
Siôn L. Williams, Jia Huang, Yvonne JK Edwards, Richard Ulla, Lloye Dillon, Tomas Prolla, Jeffery Vance, Carlos T. Moraes* and Stephan Züchner*
Cell Metabolism. 12:675-82. (2010) (*co-corresponding authors)
107. The Striatum is Highly Susceptible to Mitochondrial Oxidative Phosphorylation
Dysfunctions
Alicia M. Pickrell, Hirokazu Fukui, Xiao Wang, Milena Pinto, and Carlos T. Moraes* The Journal of Neuroscience, 31:9895-9904 (2011).
108. A metabolic shift induced by a PPAR panagonist markedly reduces the effects of
pathogenic mitochondrial tRNA mutations
Tina Wenz, Xiao Wang, Matteo Marini and Carlos T. Moraes* Journal of Cellular and Molecular Medicine, 15:2317-25 (2011)
109. Increases in Mitochondrial Biogenesis Impair Carcinogenesis at Multiple Levels
Xiao Wang, and Carlos T. Moraes* Molecular Oncology, 5:399-409 (2011)
110. Striatal Dysfunctions Associated with Mitochondrial DNA Damage in Dopaminergic
Neurons of a Mouse Model of Parkinson’s Disease
Alicia M. Pickrell, Milena Pinto, Aline Hida and Carlos T. Moraes* The Journal of Neuroscience;31:17649-58 (2011).
Curriculum Vitae Carlos T. Moraes 20
111. Manipulation of mtDNA heteroplasmy in all striated muscles of newborn mice by
AAV9-mediated delivery of a mitochondria-targeted restriction endonuclease.
Bacman SR, Williams SL, Duan D, Moraes CT*. Gene Ther.;19:1101-6 (2012).
112. Cells lacking Rieske Iron Sulfur Protein have a ROS-associated decrease in respiratory
complexes I and IV.
Diaz F, Enriquez JA, Moraes CT.* Mol Cell Biol.; 32:415-29 (2012)
113. Increase in muscle mitochondrial biogenesis does not prevent muscle loss but increased
tumor size in a mouse model of acute cancer-induced cachexia
Xiao Wang, Alicia M. Pickrell, Teresa A. Zimmers and Carlos T. Moraes* PLoS ONE; 7(3):e33426. Epub (2012)
114. Increase Mitochondrial Biogenesis in Muscle Improves Aging Phenotypes in the
mtDNA Mutator Mouse.
Lloye M. Dillon, Sion L. Williams, Aline Hida, Jacqueline D. Peacock, Tomas A. Prolla, Joy Lincoln and Carlos T. Moraes*
Human Molecular Genetics, 21:2288-97 (2012)
115. Glycolytic oligodendrocytes maintain myelin and long-term axonal integrity
Ursula Funfschilling, Lotti M. Supplie, Don Mahad, Susann Boretius, Aiman Saab, Julia Edgar, Bastian G. Brinkmann, Celia M. Kassmann, Iva D. Tzvetanova, Wiebke Moebius, Francisca Diaz, Dies Meijer, Ueli Sute, Bernd Hamprecht, Michael W. Sereda, Carlos T. Moraes, Jens Frahm, Sandra Goebbels, and Klaus-Armin Nave. Nature, 485:517-21 (2012)
116. Mitochondrial dysfunction induced by heat stress in cultured rat CNS neurons.
White MG, Saleh O, Nonner D, Barrett EF, Moraes CT, Barrett JN. J Neurophysiol.,108:2203-14 (2012).
117. A defect in the mitochondrial Complex III, but not Complex IV, triggers early ROS
dependent damage in defined brain regions.
Francisca Diaz*, Sofia Garcia, Kyle R. Padgett and Carlos T. Moraes Human Molecular Genetics, 21:5066-77 (2012)
118. Long-term bezafibrate treatment improves skin and spleen phenotypes of the mtDNA
mutator mouse.
Dillon LM, Hida A, Garcia S, Prolla TA, Moraes CT.* PLoS One,7:e44335 (2012).
119. Nitric Oxide Synthesis Is Increased in Cybrid Cells with m.3243A->G Mutation.
Gamba J, Gamba LT, Rodrigues GS, Kiyomoto BH, Moraes CT, Tengan CH. Int J Mol Sci., 14:394-410 (2012)
120. Specific elimination of mutant mitochondrial genomes in patient–derived cells by
mitoTALENs
Curriculum Vitae Carlos T. Moraes 21
Sandra R. Bacman, Siôn L. Williams, Milena Pinto, Susana Peralta and Carlos T. Moraes* Nature Medicine, 19:1111-3. (2013).
121. Mitochondrial DNA damage in a mouse model of Alzheimer’s disease decreases Aβ
plaque formation
Milena Pinto, Alicia M. Pickrell, Hirokazu Fukui and Carlos T. Moraes* Neurobiology of Aging, 34:2399-407 (2013).
122. Transient systemic mtDNA damage leads to muscle wasting by reducing the satellite
cells pool.
Xiao Wang, Alicia M. Pickrell, Susana G. Rossi, Milena Pinto, Lloye M. Dillon, Aline Hida, Richard L. Rotundo, and Carlos T. Moraes*. Hum. Mol. Genet., 22:3976-86 (2013).
123. Overexpression of Twinkle-helicase protects cardiomyocytes from genotoxic stress
caused by reactive oxygen species.
Pohjoismäki JL, Williams SL, Boettger T, Goffart S, Suomalainen A, Moraes CT, Braun T. Proc Natl Acad Sci U S A. 110:19408-13 (2013).
124. Somatic mtDNA Mutation Spectra in the Aging Human Putamen
Siôn L. Williams, Deborah C. Mash, Stephan Züchner and Carlos T. Moraes* PLoS Genet, 9(12): e1003990 (2013).
125. Mutations of cytochrome c identified in patients with thrombocytopenia THC4 affect
both apoptosis and cellular bioenergetics.
De Rocco D, Cerqua C, Goffrini P, Russo G, Pastore A, Meloni F, Nicchia E, Moraes CT, Pecci A, Salviati L, Savoia A. Biochim Biophys Acta, 1842:269-274 (2014)
126. Partial Complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a
mild encephalopathy and no increase in oxidative damage
Susana Peralta, Alessandra Torraco, Tina Wenz, Sofia Garcia, Francisca Diaz and Carlos T. Moraes*. Hum. Mol. Genet. 23:1399-412 (2014)
127. The Mitochondrial Metallochaperone SCO1 Is Required to Sustain Expression of the
High-Affinity Copper Transporter CTR1 and Preserve Copper Homeostasis Christopher J. Hlynialuk, Binbing Ling, Zakery N. Baker, Paul A. Cobine, Lisa D. Yu, Aren Boulet, Timothy Wai, Amzad Hossain, Amr M. El Zawily, Pamela J. McFie, Scot J. Stone, Francisca Diaz, Carlos T. Moraes, Deepa Viswanathan, Michael J. Petris, Scot C. Leary Cell Reports. 10, 933–943 (2015).
128. Selective Elimination of Mitochondrial Mutations in the Germline by Genome Editing
Pradeep Reddy, Alejandro Ocampo, Keiichiro Suzuki, Jinping Luo, Sandra R. Bacman, Sion L. Williams, Atsushi Sugawara, Daiji Okamura, Yuji Tsunekawa, David Lam, Nuria Monsterrat, Concepcion Rodriguez Esteban, Ignacio Sancho-Martinez, Dolors Manau, Salva Civico, Francesc Cardellach, Maria del Mar O’Callaghan, Jaume Campistol, Huimin Zhao, Josep Maria Campistol, Carlos T. Moraes, Juan Carlos Izpisua Belmonte.
Curriculum Vitae Carlos T. Moraes 22 Cell. 161, 459–469 (2015)
129. MitoTALEN: A general approach to reduce mutant mtDNA loads and restore
oxidative phosphorylation function in mitochondrial diseases.
Hashimoto M, Bacman SR, Peralta S, Falk MJ, Chomyn A, Chan DC, Williams SL, Moraes CT*. Mol Ther. 23:1592-9 (2015)
130. Cre recombinase activity is inhibited in vivo but not ex vivo by a mutation in the
asymmetric spacer region of the distal loxP site.
Arguello T, Moraes CT.* Genesis. 53:695-700 (2015)
131. Enhanced Transcriptional Activity and Mitochondrial Localization of STAT3 Co-
induce Axon Regrowth in the Adult Central Nervous System
Xueting Luo, Marcio Ribeiro, Eric R. Bray, Do-Hun Lee, Benjamin J. Yungher, Saloni T. Mehta, Kinjal A. Thakor, Francisca Diaz, Jae K. Lee, Carlos T. Moraes, John L. Bixby, Vance P. Lemmon, Kevin K. Park. Cell Reports. 15:398-410 (2016)
132. Pioglitazone ameliorates the phenotype of a novel Parkinson's disease mouse model by
reducing neuroinflammation.
Pinto M, Nissanka N, Peralta S, Brambilla R, Diaz F, Moraes CT*. Mol Neurodegener. 11(1):25 (2016)
133. Sustained AMPK activation improves muscle function in a mitochondrial myopathy
mouse model by promoting muscle fiber regeneration Susana Peralta, Garcia Sofia, Han Yang Yin, Arguello Tania, Francisca Diaz and Carlos T. Moraes*. Hum. Mol. Genet. 25(15):3178-3191 (2016).
134. The CoQH2/CoQ Ratio Serves as a Sensor of Respiratory Chain Efficiency. Guarás A, Perales-Clemente E, Calvo E, Acín-Pérez R, Loureiro-Lopez M, Pujol C, Martínez-Carrascoso I, Nuñez E, García-Marqués F, Rodríguez-Hernández MA, Cortés A, Diaz F, Pérez-Martos A, Moraes CT, Fernández-Silva P, Trifunovic A, Navas P, Vazquez J, Enríquez JA. Cell Rep.15:197-209 (2016).
135. Transient mitochondrial DNA double strand breaks in mice cause accelerated aging
phenotypes in a ROS-dependent but p53/p21-independent manner.
Milena Pinto, Alicia M. Pickrell, Xiao Wang, Sandra R. Bacman, Aixin Yu, Aline Hida, Lloye M. Dillon, Paul D. Morton, Thomas R. Malek, Siôn L. Williams, and Carlos T. Moraes*. Cell Death and Differ. 24:288-299 (2017).
136. Phosphorylation of Cytochrome c Threonine 28 Regulates Electron Transport Chain
Activity in Kidney: Implications for AMP Kinase
Curriculum Vitae Carlos T. Moraes 23
Gargi Mahapatra, Ashwathy Varughese, Qinqin Ji, Icksoo Lee, Jenney Liu, Asmita Vaishnav, Christopher Sinkler, Alexandr A Kapralov, Carlos T Moraes, Thomas H Sanderson, Timothy L Stemmler, Lawrence I Grossman, Valerian E Kagan, Joseph S Brunzelle, Arthur R Salomon, Brian F.P. Edwards, and Maik Hüttemann J. Biol. Chem. 292:64-67 (2017).
137. Respiration-Deficient Astrocytes Survive as Glycolytic Cells In Vivo.
Lotti Marianna Supplie, Tim Du king, Graham Campbell, Francisca Diaz, Carlos T. Moraes,
Magdalena Gotz, Bernd Hamprecht, Don Mahad and Klaus-Armin Nave
J. Neurosci. 37:4231-4242 (2017).
138. Mitochondrial DNA Double-Strand Breaks in Oligodendrocytes Cause Demyelination,
Axonal Injury, and CNS Inflammation. Madsen PM, Pinto M, Patel S, McCarthy S, Gao H, Taherian M, Karmally S, Pereira CV, Dvoriantchikova G, Ivanov D, Tanaka KF, Moraes CT, Brambilla R. J Neurosci. 37:10185-10199 (2017).
139. Lack of Parkin anticipates the phenotype and affects mitochondrial morphology and
mtDNA levels in a mouse model of Parkinson’s Disease.
Milena Pinto, Nadee Nissanka, Carlos T. Moraes* J Neurosci. 38:1042-1053 (2018).
140. Overexpression of PGC-1 in aging muscle enhances a sub-set of young-like molecular
patterns. Sofia Garcia, Nadee Nissanka, Edson A. Mareco, Susana Rossi, Susana Peralta, Francisca Diaz, Richard L. Rotundo, Robson F. Carvalho, Carlos T. Moraes*. Aging Cell 17(2). doi: 10.1111/acel.12707 (2018)
141. SCO2 mutations cause early-onset axonal Charcot-Marie-Tooth disease associated
with cellular copper deficiency.
Rebelo AP, Saade D, Pereira CV, Farooq A, Huff TC, Abreu L, Moraes CT, Mnatsakanova D, Mathews K, Yang H, Schon EA, Zuchner S, Shy ME. Brain. 141(3):662-672. (2018)
142. The Mitochondrial DNA Polymerase Gamma Degrades Linear DNA Fragments
Precluding the Formation of Deletions Nadee Nissanka, Sandra R. Bacman, Melanie J. Plastini, and Carlos T. Moraes* Nature Communications 9(1):2491. doi: 10.1038/s41467-018-04895-1 (2018).
143. MitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial
DNA levels Claudia V. Pereira, Sandra R. Bacman, Tania Arguello, Ugne Zekonyte, Sion L. Williams,
David R. Edgell and Carlos T. Moraes* EMBO Mol. Med. pii: e8084. doi: 10.15252/emmm.201708084 (2018).
144. ATAD3 controls mitochondrial cristae structure, influencing mtDNA replication and
cholesterol levels in muscle
Curriculum Vitae Carlos T. Moraes 24 Susana Peralta, Steffi Goffart, Sion L. Williams, Francisca Diaz, Sofia Garcia, Nadee
Nissanka, Estela Area-Gomez, Jaakko Pohjoismäki, Carlos T. Moraes* J. Cell Sci 131(13). pii: jcs217075. doi: 10.1242/jcs.217075 (2018).
145. Mitochondrial methionyl N-formylation affects oxidative phosphorylation complexes
steady-state levels and their organization into supercomplexes. Arguello T, Köhrer C, RajBhandary UL, Moraes CT.* J Biol Chem. pii: jbc.RA118.003838. doi: 10.1074/jbc.RA118.003838 (2018).
146. Ablation of cytochrome c in adult forebrain neurons impairs oxidative
phosphorylation without detectable apoptosis.
Pinto Milena, Uma D. Vempati, Francisca Diaz, Susana Peralta, Carlos T. Moraes*. Mol Neurobiol. doi: 10.1007/s12035-018-1335-y (2018).
147. MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse
model of heteroplasmic mtDNA mutation
Sandra R. Bacman, Johanna H. K. Kauppila, Claudia V. Pereira, Nadee Nissanka, Maria Miranda, Milena Pinto, Sion L. Williams, Nils- Göran Larsson, James B. Stewart and Carlos T. Moraes*
Nature Medicine doi: 10.1038/s41591-018-0166-8. (2018). 20. Other work accepted for publication:
PROFESSIONAL
21. Funded research:
Active
PI- Moraes, C.T. Mitochondrial Dysfunction in Neurodegeneration and Compensatory Approaches The National Institute of Health (NIA, 1R01AG036871-06). June/1/10 - May/03/20 Direct costs: $245,000/Year
PI- Moraes, C.T. Setting the stage for replacement of mitochondrial genes The National Institute of Health (NEI, 5R01EY010804-19). Aug/1/95 - April/30/21 Direct costs: $295,000/Year
Co-PI (Caicedo-A and Moraes,C.T.) Imaging mitochondrial signaling in B-cells ectopically implanted in the eye. The National Institute of Health (NIEHS, 1R33ES025673) May/1/2015-September 14/2020
Direct costs: $250,000/Year
PI- Moraes, C.T. Monomeric Gene Editing Enzymes to Treat Mitochondrial Myopathies (MDA573406),"
Curriculum Vitae Carlos T. Moraes 25
Muscular Dystrophy Association. August/1/2018 – July/31/2021 Direct Costs: 99,805/year
Past
PI- Moraes, C.T.
Mouse Models with Defective Respiratory Complexes in the CNS The National Institute of Health (NINDS, 1R01NS079965) July/1/2012-June/30/2017 Direct costs: $219,000/Year
PI- Moraes, C.T.
Reducing the levels of mtDNA mutations by mitochondrial nucleases The Muscular Dystrophy Association (May/1/14-April/30/17)
Direct Costs: $100,000/year PI- Moraes, C.T. Developing specific mitochondrial nucleases to eliminate mutant mtDNA United Mitochondrial Disease Foundation (September 2014-August 2016) Direct Costs: $60,000/year PI- Moraes, C.T. The Role of Mitochondrial Oxidative Phosphorylation Dysfunction in Alzheimers Ed and Ethel Moore Alzheimer¹s Disease Research Program (FL Biomedical Research Foundation) (January 2015-June 2015) Direct Costs: $200,000
PI- Moraes, C.T. Increased Mitochondrial Biogenesis as Therapy to Mitochondrial Myopathies The Muscular Dystrophy Association (July/1/10-June/30/13)
Direct Costs: $113,000/year PI- Moraes, C.T.
Mouse Models with Defective Respiratory Complexes in the CNS The National Institute of Health (NINDS, 5R56NS041777). Sept/1/10 - Aug/30/11 Direct costs: $277,000/Year (no-cost extension to Aug/30/2012)
PI- Barrientos, A. Co-PI- Moraes, C.T.
Mitochondrial Biogenesis in Florida Biomedical Research Foundation (January/10-December/11)
Direct Costs: $330,000/year
PI- Moraes, C.T. The role of oxidative phosphorylation in cell growth and death The National Institute of Health (NCI, 2R01CA085700-05). Dec/1/00 - Nov/30/10 Direct costs: $163,000/year PI- Moraes, C.T.
Curriculum Vitae Carlos T. Moraes 26 Mouse Models with Defective Respiratory Complexes in the CNS The National Institute of Health (NINDS, 5R01NS041777). Apr/1/01 - March/31/10 Direct costs: $250,000/year PI- Moraes, C.T. Mitochondrial involvement in Parkinson Disease The Parkinson Disease Foundation (July/08-June/09) Direct Costs: $50,000/year
PI- Moraes, C.T. Studying and Protecting Motor Neuron’s Mitochondria in ALS The Muscular Dystrophy Association. July/1/03 - September/30/06 Direct costs: $90,000/year
PI- Moraes, C.T. Exploring nuclear-mitochondrial interactions The National Institute of Health (NIGMS, 5R01GM055766-08). July/1/97 - August/30/05
(no-cost extension to 8/06) Direct costs: $200,000/year
PI- Moraes, C.T.
Studying and Protecting Motor Neuron’s Mitochondria in ALS The Muscular Dystrophy Association. July/1/00 - June/30/03 Direct costs: $70,000/year
PI- Moraes, C.T.
Detection and Molecular Analysis of Pathogenic Mitochondrial DNA Mutations The Muscular Dystrophy Association. July/1/97 - June/30/00 Direct costs: $50,000/year PI- Moraes, C.T.
Developing an animal model of Parkinson Disease The National Parkinson Foundation July/1/00-June/30/01
Direct costs: $40,000/year
PI- Moraes, C.T. Pew Scholar in the Biomedical Sciences. July/1/95 - June/30/99 Direct costs: $50,000/year PI- Moraes, C.T. Mitochondrial Complex I Deficiency in Parkinson's Disease National Parkinson Foundation August/1/ 95 - July/31/ 96 Direct costs: $39,627/year PI- Moraes, C.T. Detection and Molecular Analysis of Pathogenic Mitochondrial DNA Mutations The Muscular Dystrophy Association. July/1/94 - June/30/97 Direct costs: $176,000
Curriculum Vitae Carlos T. Moraes 27 22. Editorial Responsibilities: Special Editor for mitochondrial DNA mutations reports- Neuromuscular Disorders (1994-
2000). Assistant Editor - Mitochondrion (2000-present) Editorial Board – Human Molecular Genetics (2008-present)
Ad hoc reviewer for: Neurology, Proceedings of the National Academy of Sciences, Nature Genetics, Science, Science Translational Medicine, Molecular Cell, Cell, Cell Metabolism, American Journal of Human Genetics, Annals of Neurology, PLoS Genetics, Molecular and Cellular Biology, Human Molecular Genetics, Analytical Biochemistry, American Journal of Medical Genetics, Journal of Neurochemistry, Journal of Neuroscience, Pediatric Research, Journal of Neuropathology and Experimental Neurology, Mitochondrion, Genes Chromosomes and Cancer, Biotechniques, Nucleic Acids Research, and Neuromuscular Disorders.
23. Professional organizations: American Society for the Advancement of Science, The
American Society of Human Genetics (1995-2010). American Society of Gene & Cell Therapy: ASGCT (2014-2016).
24. Awards and Honors
External Review Committee. Welcome Centre for Mitochondrial Research. Newcastle, UK (November 2018).
Scientific Advisory Board, Max Planck institute for the Biology of Aging, Cologne, Germany. (2012-2018)
Member of Scientific Advisory Committee. Muscular Dystrophy Association. [1999-2004]; [2007- present]
DOD Grant Review Member (October 2015). NIEHS Intramural Program Review Committee (Ad Hoc). July 2015 and November 2017. NINDS Neurological Sciences and Disorders B (NSD-B) Review Group (Regular Member)
2014-2018 FASEB Summer Research Conference on “Mitochondrial Assembly and Dynamics in Health,
Disease and Aging” Organizer (May 2017). FDA Advisor for mtDNA manipulation in Reproductive Techniques. Cellular, Tissue and Gene
Therapies Advisory Committee meeting on Feb. 25-26, 2014. Esther Lichtenstein Professor of Neurology Chair, January 2014-present External Advisory Review member. Program Project at the Houston Merritt Center at
Columbia University (2009-2015). NIH-UMDF Workshop on Primary Mitochondrial Disorders (Co-Chair). Bethesda, March 2012. NIH Neural Oxidative Metabolism and Death (NOMD) Review Group (Regular Member) 2010-
2013 United Mitochondrial Disease Foundation- Chair, Scientific and Medical Advisory Board
(2009-2012) United Mitochondrial Disease Foundation- Chair, Annual Meeting Scientific Program
Committee (2010) United Mitochondrial Disease Foundation Scientific and Medical Advisory Board (2006-2012) NIH ZRG1 MOSS-L (07). Muscle Biology Special Emphasis Panel. 2008
Curriculum Vitae Carlos T. Moraes 28 Provost Award for Scholarly Activity, University of Miami, 2005 NIH Mammalian Genetic (Now Genetics of Health and Disease [GHD]) Review Group
(Regular Member) 2002-2006 NIH Mammalian Genetic Review Group (Ad Hoc). 2001 NIH ZRG1 SSS5(02) Special Emphasis Panel. 2003 NIH Molecular Cytology Special Emphasis Panel. 1998 NIH Chemistry and Related Sciences Special Emphasis Panel. 1998 National Eye Institute (NIH). Committee on "Development of a National Plan for Vision
Research 1999-2003" National Heart, Lung, and Blood Institute (NIH). Scientific Review Committee for RFA: HL-
96-013 (Mitochondrial DNA Mutations in Heart, Lung and Blood) 1997 VA ad hoc Merit Grants Reviewer (1996) Pew Scholar in the Biomedical Sciences (1995-1999). The Samuel W. Rover and Lewis Rover Award for Research in Genetics & Development. 5/93.
25. Post-doctoral fellowships: none 26. Invited Lectures
1. Large-scale deletions of mitochondrial DNA in patients with Neuromuscular diseases.
Pan-American Association of Biochemical Societies (PAABS) VI Congress. São Paulo, Brazil. 2/90
2. New genetic abnormalities of mitochondrial DNA in mitochondrial diseases
1991 Annual Meeting of the Society for Inherited Metabolic Disorders. Santa Fé, New Mexico, USA. 4/91
3. Quantitative errors of mtDNA in mitochondrial diseases
Meeting on Mitochondrial Encephalomyopathies: Defects of Mitochondrial DNA International School of Neurological Sciences, S. Servolo, Venice, Italy. 11/91
4. Mitochondrial Disorders: Defects of mitochondrial DNA
Congress of the International Association of Child Neurology. Buenos Aires, Argentina 11/92
5. Quantitative defects of mitochondrial DNA
Third Annual Miami Children's Hospital Research Institute Symposium: "Genetics, Neurobiology and Nutrition in Human Disease: Prevention, Treatment and Future Directions". Coral Gables, Florida. 12/92
6. Morphological features in the diagnosis of mitochondrial encephalo-myopathies.
45th Annual Meeting of the American Academy of Neurology. New York, NY. 5/93.
7. The molecular basis of neuromuscular disorders
International symposium in neurosciences. Goiania, Brazil. 11/93
8. Morphological features in the diagnosis of mitochondrial encephalomyopathies.
46th Annual Meeting of the American Academy of Neurology. Washington, DC. 5/94.
9. Mitochondrial DNA defects in Inclusion Body Myositis
Symposium on diagnostic criteria, definitions, pathogenesis and treatment of sporadic and hereditary IBM. Jupiter, Florida, 3/94
10. Cytochrome oxidase deficiency and mtDNA depletion
The VIII International Congress on Neuromuscular Diseases. Kyoto, Japan, 7/94
11. Molecular analysis of the muscle pathology associated with mtDNA deletions in CPEO
Glycolitic and Mitochondrial Defects Symposium, Osaka, Japan, 7/94
Curriculum Vitae Carlos T. Moraes 29
12. Recent Advances on the molecular pathogenesis of mitochondrial disorders
International Meeting of the Scandinavian Association of Clinical Chemists. Stockholm, Swedeen. 8/94.
13. Morphological features in the diagnosis of mitochondrial encephalo-myopathies.
47th Annual Meeting of the American Academy of Neurology. Seattle, WA. 5/95.
14. Mitochondrial oxidative mass: Evolution from a molecular perspective.
Workshop symposium of the International Society for the Study of Fatty Acids and Lipids at the National Institute of Health, Bethesda, MD. 6/95.
15. Mitochondrial disorders: a naturally occurring collection of mutants for the study of
mitochondrial biogenesis. Simposio Areces Enfermedades Del DNA Mitocondrial. Zaragoza, Spain. October 19 - 20, 1995
16. Morphological features in the diagnosis of mitochondrial encephalo-myopathies.
48th Annual Meeting of the American Academy of Neurology. San Francisco, CA. 3/96.
17. Molecular Basis of Mitochondrial Disorders
13th annual meeting of the Southern Association for Neurology and Neuroradiology. Porto Alegre, Brazil 11/96
18. Morphological features in the diagnosis of mitochondrial encephalo-myopathies.
49th Annual Meeting of the American Academy of Neurology. Boston, MA. 4/97.
19. Mitochondrial disorders
Satellite meeting to the International Congress in Neuromuscular Disorders Melbourne, Australia, 1998
20. Evolutionary constraints in nuclear-mitochondrial interactions
EUROMIT IV. Queens College, Cambridge, UK September/1999
21. Complex I deficiencies: Cellular consequences.
Annual Clinical Genetics Meeting, Palm Springs, CA March/2000.
22. Nuclear-Mitochondrial interactions in FREAK cells.
Dept. of Physiology. Baylor College of Medicine, Houston, September, 2000.
23. Clinical and Pathophysiological Analogy of Genetic mtDNA depletion to the Nucleoside
analogue-induced mtDNA depletion.
Workshop in HIV Neuropathy. Washington DC, September 2000.
24. Nuclear-Mitochondrial interactions in xenomitochondrial cybrids
Children’s National Medical Center Research Institute, Washington DC, February 2001
25. Mitochondrial DNA replication
American Society of Neurochemistry Annual Meeting. Colloquium: Mitochondria-Beyond Bioenergetics. Palm Beach, FL June 2002
26. Animal Models of Electron Transport Defects
EUROMIT VI. Nijmegen, The Netherlands. July/2004
27. OXPHOS Complexes homeostasis
Gordon Research Conference “Molecular and Cellular Bioenergetics” University of New England. June/2005
28. Mouse models of OXPHOS deficiency in the CNS
Banbury Research Conference Cold Spring Harbor. October/2005
29. Genetic Therapies for Mitochondrial Myopathies
International Society for Ophthalmology São Paulo, Brazil, February/2006
30. Mouse models of cytochrome oxidase deficiencies
First International Colloquium on Leigh’s syndrome French Canadian
Curriculum Vitae Carlos T. Moraes 30
Montreal. May/2006
31. Modulating mtDNA heteroplasmy with restriction endonucleases
Mastering the mitochondrial maize (UMDF annual meeting) Atlanta. June/2006
32. Gene Therapy for Mitochondrial Disorders
International Conference on Mitochondrial Biomedicine & Chinese Mit’ 2006: Mitochondria and Health & Asian Society for Mitochondriasel Rearch and Medicine Workshop. Wenzhou, Zhejiang Province, China , November/2006
33. Animal Models and Therapeutic Approaches to Mitochondrial Diseases.
Scripps Florida. November/2006.
34. New Advances in Mitochondrial Diseases.
Neurology Update Course, Miami Beach. January/2007
35. Cellular and Molecular Mechanisms of Energy Metabolism in the Brain: Relevance to
Brain Disorders.
Evelyn F. McKnight Brain Research Foundation Symposium. University of Miami Miller School of Medicine. April/2007
36. Interdependence of Oxidative Phosphorylation Components for Assembly and Stability.
10th IUBMB and XXXVI Annual Meeting of SBBq. Salvador, Brazil. May/2007
37. OXPHOS Biogenesis.
FASEB symposium on Mitochondrial Biology, Tucson, AZ. August/2007
38. Cytochrome oxidase deficiencies and Alzheimers Disease
ScandMit symposium Tampere, Finland. October/2007
39. Increased mitochondrial biogenesis as treatment to mitochondrial myopathies
Howard Hughes Medical Institute Janelia Farm Conference. March/2008
40. Using Mouse models to Develop Novel Therapies for Mitochondrial Diseases
Florida Atlantic University Neuroscience series. Boca Raton, FL. September/2008
41. Mitochondrial involvement in neurodegenerative diseases
Udall NIH Centers annual Meeting, Chicago. IL. October/2008
42. Novel Therapeutic Approaches to Mitochondrial Diseases
Max Planck Institute, Neuroscience Series. Göttingen, Germany. November/2008
43. Mitochondrial dysfunction and oxidative damage: Is the dogma crumbling?
Nature Conferences (EC), Nature Genetics/Nature Neurosciences/Fondation IPSEN. Mitochondrial Dysfunction in Neurological Disease. Durham, NC. December/2008
44. Alzheimer’s disease and mitochondrial dysfunction.
Aging, Neurodegeneration ansd Mitochondria. Juselius Foundation, Helsinki, Finland. March 2009.
45. The role of MTERF2 in mitochondrial transcription.
United Mitochondrial Disease Foundation Annual Meeting. Washington DC. June 2009
46. Mitochondrial Biogenesis in degenerative diseases.
Grand Rounds, Dept. of Pathology, Yale University. New Haven, CT. October 2009
47. PGC-1 and aging.
“Current Diagnostics and Therapeutic Issues on neuromuscular Disorders”, Athens, Greece. November 2009
48. Mitochondrial Biogenesis in mitochondrial diseases.
Mitochondial Research Affinity Group, CHOP, University of Pennsylvania.. Phyladelphia, PA. December 2009.
49. Mitochondrial diseases.
Curriculum Vitae Carlos T. Moraes 31 Department of Medicine Lecture. Northwestern Medical School, department of Medicine,
Chicago. May 2010.
50. Mitochondrial biogenesis in aging.
Invited Lecture at the Max Planck Institute on Aging Research, Cologne, Germany. April 2010.
51. Mitochondrial Biogenesis in mitochondrial diseases.
Invited speaker at the Experimental Biology symposium on Mitochondria and Aging, Anaheim, CA, April 2010
52. Mitochondrial Biogenesis in aging.
United Mitochondrial Disease Foundation Annual Meeting. Scottsdale AZ. June 2010
53. mtDNA variations in the mutator mouse
Gordon Research Conference “Mitochondrial and Chloroplasts” Il Ciocco, Tuscany, Italy. July/2010
54. Mitochondria-targeted restriction endonucleases as tools to understand and treat
mitochondrial diseases.
New Frontiers in Bioenergetics symposium. University Nijmegen Medical Centre, Netherlands. November 2010.
55. Biological advances in aging research.
Global Business Forum, University of Miami, Coral Gables. January 2011
56. Mitochondrial Biogenesis and Aging: The more the merrier.
Discovery Grand Rounds, University of Miami, March 2011
57. Mitochondrial DNA Defects in Development.
ASBMB-EB 2011, Washington, DC. April 2011
58. Mitochondrial Biogenesis and Sarcopenia.
ASP-EB 2011, Washington, DC. April 2011
59. Mitochondrial Aging
Florida Atlantic University Neuroscience series. Boca Raton, FL. April/2011
60. Mitochondrial Biogenesis in Aging and Disease.
University of Central Florida Neuroscience series. Orlando, FL. May/2011
61. Mitochondrial Biogenesis and Aging: The more the merrier.
Howard Hughes Medical Institute Janelia Farm Conference. May/2011
62. Mitochondrial Biogenesis as Therapy to Mitochondrial Disorders.
EuroMit 2011, Zaragoza, Spain. June 2011
63. Mitochondria and Aging.
Gordon Conference on Bioenergetics. Proctor College, NH. June 2011
64. Mitochondria Dysfunction and Diseases.
International Symposium of the German Society for Biochemistry and Molecular Biology (GBM). Frankfurt am Main, Germany. September 2011
65. Novel Therapeutic Approaches to Mitochondrial Diseases.
Treatment of mitochondrial disease - now and in the future. Founding meeting for the International Coordinating Committee for Mitochondrial Diseases (ICCMD). Bethesda, MD. October 2011.
66. Workshop- Barriers and Opportunities in Mitochondrial Diseases.
Chair and speaker on “Model Systems of Mitochondrial Diseases” Sponsored by the NIH and the UMDF. Bethesda, MD. March 2012
67. Mitochondrial Aging.
XLI Annual Meeting of the Brazilian Biochemistry and Molecular Biology Society (SBBq), Foz do Iguaçu, Paraná, Brazil, May 2012.
Curriculum Vitae Carlos T. Moraes 32
68. Therapeutic Approaches to Mitochondrial Diseases.
Invited Seminar. Department of Pathology, University of Pittsburgh, Pittsburgh, PA. January 2013.
69. Treating mitochondrial diseases
MDA-Sponsored Therapeutic Approaches to Muscle Diseases Bethesda, April 2013.
70. Novel Genetic Approaches to Treat Mitochondrial Diseases.
NHLB-Sponsored conference on mitochondrial biology. Bethesda, May 2013.
71. Mouse Models of Mitochondrial Diseases.
International Symposium: "Mitochondrial diseases. Madrid, May 2013
72. Mitochondrial DNA Heteroplasmy Manipulation with mitoTALEN.
United Mitochondrial Disease Foundation Annual Meeting. Orange County CA. June 2013
73. p53 activation after mitochondrial DNA double strand breaks.
FASEB meeting in Mitochondrial Biogenesis. Big Sky, Montana MT. June 2013
74. Modifying mitochondrial genomes
Max Planck Institute for Biophysics. Bad Neuheim, Germany. October 2013
75. Mitochondrial DNA damage in muscle aging
EMBO workshop on Muscle Wasting. Ancona, Switzerland. September 2013.
76. Mitochondrial DNA gene therapy.
Mitochondrial Disease: Translating biology into new treatments. Wellcome Trust Scientific Conferences. Cambridge, UK, October 2013.
77. mtDNA damage in aging.
Department of Genetics Seminar Series. McGill University, Montreal November 5th, 2013
78. Mitochondrial DNA double strand breaks and aging.
Mitochondrial Driven Mechanisms of Redox Signaling in Disease November 20 - 24, 2013, San Antonio, TX (SFRBM 2013).
79. Genetic Therapy for mtDNA Disorders.
Mitochondria, Energetics and Metabolism meeting. 27th-30th January 2014. Mahabaleshwar, India.
80. Manipulating Mitochondrial DNA Heteroplasmy in vivo to Treat Mitochondrial
Diseases.
Center for Molecular Medicine and Genetics Seminar Series. Wayne State University, Detroit. March 20th, 2014.
81. Rethinking the Cellular Targets of Mitochondrial Dysfunction Associated with Aging
American Aging Association 43rd Annual Meeting. San Antonio, TX May 30th, 2014
82. Engineered Mitochondrial Nucleases for the Treatment of Mitochondrial DNA
Disorders.
United Mitochondrial Disease Foundation Annual Meeting, Pittsburgh, PA. June 5th, 2014
83. Mitochondrial TAL effector nucleases as therapy for mitochondrial diseases.
EuroMit 2014, Tampere, Finland. June 2014
84. Course in Mitochondrial Medicine, Bertinoro di Romagna, Italy. December 3, 2014.
85. Genetic Treatment for Mitochondrial Diseases Caused by Heteroplasmic mtDNA
Mutations.
Symposium “Links in Metabolism and Mitochondria in Aging Diseases and Their Therapeutic Potentials”. Gainsville, FL. December 2014.
86. Mitochondrial DNA damage in aging. Workshop on "Mitochondria and Metabolic Signaling in Ataxia-Telangiectasia". Tarrytown, NY. December 2014.
Curriculum Vitae Carlos T. Moraes 33
87. Recent Advances in the Genetics of Mitochondrial Disorders: From Diagnosis to
Therapy. Neurology Update and Stroke Intensive 2015. Miami, FL. January 2015.
88. Selective Targeting of MtDNA Sequences and Applications to Therapy. 59th Annual Biophysical Society Meeting. Baltimore, MD. February 2015.
89. Targeting Disease-Causing Defects of the Mitochondrial Genome with Engineered
Mitochondrial Nucleases. Targeting Mitochondrial Dysfunction & Toxicity Conference (Healthtech.com)., Boston MA. March 2015.
90. Potential alternative to preventing transmission of mtDNA diseases: heteroplasmy shift
therapy. Institute of Medicine Public Workshop of the Committee on Ethical and Social Policy Considerations of Novel Techniques for Prevention of Maternal Transmission of Mitochondrial DNA Diseases. Washington, DC. April 2015.
91. MitoTALENS: A general approach to reduce mutant mtDNA loads and restore
oxidative phosphorylation function in mitochondrial diseases. FASEB Meetings. Palm Beach, FL. May 2015.
92. Therapeutic Approaches to Mitochondrial Defects in Muscle. Gordon Conference in Myogenesis. Barga, Italy. June 2015.
93. The use of mitochondrial-targeted nucleases to alter mtDNA heteroplasmy. MitoCross Symposium 2015, Strasbourg, France. September 2015.
94. Manipulating mitochondrial DNA heteroplasmy with designer nucleases. Baylor College of Medicine, Dept of Genetics Seminar Series. Baylor, TX. January 2016.
95. Pharmacological and genetic approaches to treat mitochondrial diseases. Workshop :Implication of metabolic and epigenetic modifications for neuroprotection: relevance to translational research. Fundação Santa Casa. São Paulo, Brazil. March 2016.
96. Messing with mtDNA in vivo using mitochondrial targeted nucleases. Molecular biology of mitochondrial gene expression. Bro, Sweden. May 2016.
97. Modulating mtDNA Heteroplasmy with Designer Nucleases. United Mitochondrial Disease Foundation Annual Meeting, Seattle, WA. June, 2016
98. Mitochondrial DNA Double-Strand Breaks: Consequences and Uses. University of Nebraska-Lincoln Biochemistry Department and Redox Biology Center. September 2016.
99. Manipulating the Mitochondrial Genome with Designer Nucleases. Genome Editing for Gene and Cell Therapy, a Herrenhausen Symposium. Hanover, Germany. November, 2016.
100. Biochemical and genetic basis of mitochondrial encephalopathies. From Pediatric Encephalopathy to Alzheimer's: Linking Mitochondria to Neurological Disease. Neurobiology of Disease Workshop. Society for Neuroscience Annual Meeting. San Diego, CA. November, 2016.
101. Mitochondrial DNA double strand breaks cause a premature aging-like phenotype in
mice. Stem Cell Institute Seminar Series. University of Minnesota. Minneapolis, MN. November, 2016.
102. Manipulating mtDNA with TALEN-based nucleases. Annual UK MRC Centre Translational Research Conference. Institute of Child Health, London. March 2017.
103. The role of methionine formylation in OXPHOS synthesis and assembly. MRC Center for Mitochondrial Research. Cambridge, UK. March 2017.
104. Targeted for Destruction: Gene Therapy for mtDNA diseases. Wellcome Trust Centre for Mitochondrial Research, Newcastle University, UK. March 2017.
105. Modulating mtDNA heteroplasmy in vivo with gene editing enzymes. EUROMIT 2017. Cologne, Germany. June 2017.
106. Advances on the Use of Designer Nucleases to Reduce Mutant mtDNA Levels. United Mitochondrial Disease Foundation Annual Meeting, Washington DC. June 2017.
Curriculum Vitae Carlos T. Moraes 34
107. Novel Methods of mtDNA Modulation.
American Physiological Society. Special Symposium of Mitochondrial function. San Diego, August 2017.
108. Manipulating mtDNA with mitochondrial-targeted nucleases.
Cold Spring Harbor Asia- Mitochondria. Suzhou, China, October 2017.
109. Development of Genetic Treatments to Mitochondrial Diseases
Workshop in Genetics-Universidade Estadual de São Paulo-Botucatu. April 2018.
110. Consequences of mtDNA double-strand Breaks in vivo. EMBO Workshop on mitochondrial gene expression. Sånga Säby, Sweden. June 2018.
111. Development of Genetic Therapy for mtDNA Diseases. Lecture at the Dept. of Environmental Sciences, Florida International University, Miami. August 2018.
112. MtDNA breaks and deletions: Clinical Implications. Heartland Center for Mitochondrial Medicine symposium. Kansas City, September 2018.
113. Development of Genetic Therapy for mtDNA Diseases. World Muscle Society Congress, Mendoza, Argentina. October 2018.
114. Consequences and therapeutic uses of mtDNA double-strand breaks in mammalian
cells. Cold Spring Harbor Laboratory Seminar. THE EVOLVING CONCEPT OF
MITOCHONDRIA: FROM SYMBIOTIC ORIGINS TO THERAPEUTIC OPPORTUNITIES. Cold Spring Harbor, NY. October 2018.
115. International Scientific Advisory Board Symposyum. Mitochondrial DNA editing enzymes. Newcastle, UK. November 2018.
TEACHING
27. Teaching awards received: None 28. Teaching Specialization (classes taught): Mitochondria function and dysfunction in neurological diseases (NEU631). (co-organizer)
Fall 2014 Mitochondrial biogenesis and genetics (PIBS601). Annually, starting in 1995-present (one lecture/ year) PIBS602 (two lectures/year) 2010-present Mitochondrial genetics (Genetics) 2001-2002
Clinical/biochemical correlations (Biochemistry and Molecular Biology, 1995) Research Opportunities in Human Diseases (Molecular Pharmacology, 1995) Molecular Pathology of Mitochondrial Disorders (Neurological Update/ School of continuing
education UM) Annually, starting in 1994 Morphological approaches to mitochondrial disorders (Mitochondrial disorders half-day
course/ American Academy of Neurology) 1993-1998 29. Thesis advising:
Celia Harumi Tengan, M.D., Ph.D. Ph.D. Program in Neuroscience through a collaboration between the Escola Paulista de
Medicina (São Paulo, Brazil), and the University of Miami (1994-1996). Ilias Kirkinezos Graduate Program in Molecular, Cell and Dev. Biol, University of Miami (1997-2003) Sarika Srivastava
Curriculum Vitae Carlos T. Moraes 35
Graduate Program in Molecular, Cell and Dev. Biol, University of Miami (1998-2005) Corina van Waveren Graduate Program in Molecular, Cell and Dev. Biol, University of Miami (2002-2005) Corneliu Luca Graduate Program in Molecular, Cell and Dev. Biol, University of Miami (2003-2008) Hirokazu Fukui Graduate Program in Neuroscience, University of Miami (2004-2008) Adriana Rebelo
Graduate Program in Molecular, Cell and Dev. Biol, University of Miami (2005-2009) Alicia Pickrell
Graduate Program in Neuroscience, University of Miami (2007-2012) Lloye Dillon
Graduate Program in Molecular, Cell and Dev. Biol, University of Miami (2007-2012) Xiao Wang
Graduate Program in Cancer Biology, University of Miami (2008-2012) Tania Arguello
Graduate Program in Human Genetics, University of Miami (2012-2017) Nadee Nissanka
Graduate Program in Neuroscience, University of Miami (2013-2018) Ugne Zekonyte
Graduate Program in Human Genetics, University of Miami (2017-) Christian Gonzalez
MD/PhD Program, University of Miami (2018-) Post-doctoral fellows supervision Ashok Verma MD (postdoctoral). University of Miami 1995-1998. David Atencio Ph.D. (post-doctoral). University of Miami 1995-1999. Antoni Barrientos Ph.D. (post-doctoral) University of Miami 1997-1999. Runu Dey, Ph.D. (post-doctoral) University of Miami 1997-2000. Francisca Diaz, Ph.D. (post-doctoral) University of Miami 1999-2003
Markus Woischnick, Ph.D. (post-doctoral) University of Miami 2000-2002 M. Pilar Bayona, Ph.D.. (post-doctoral) University of Miami 2001-2005 Sandra Bacman, Ph.D.. (post-doctoral) University of Miami 2001-2005 Uma Vampati, Ph.D. (post-doctoral) University of Miami 2002-2009 Sion Williams, Ph.D. (post-doctoral) University of Miami 2005-2009 Tina Wenz, Ph.D. (post-doctoral) University of Miami 2005-2009 Alessandra Torraco, Ph.D. (post-doctoral) University of Miami 2006-2008 Sarika Srivastava, Ph.D. (post-doctoral) University of Miami 2006-2007 Milena Pinto, Ph.D. (post-doctoral) University of Miami 2010-2017 Susana Peralta, Ph.D. (post-doctoral) University of Miami 2010-2017 Claudia Pereira, Ph.D. (post-doctoral) University of Miami 2013-present Masami Hashimoto, Ph.D. (post-doctoral) University of Miami 2012-2015 Tania Arguello, Ph.D. (post-doctoral) University of Miami 2017-present
SERVICE
Curriculum Vitae Carlos T. Moraes 36 30. University Committee and Administration: Committee member "Research Liaison" with the Dean for Research 1997-1998 Committee member, Executive Promotions, University of Miami School of Medicine 1998-
2004 Steering Committee member. Graduate Program in Molecular, Cell and Developmental
Biology. 2000-2008 Steering Committee member. Graduate Program Human Genetics. 2008-2011 Steering Committee member. Graduate Program Neurosciences. 2011-present
Chair of the Faculty Research Advisory Committee (FRAC) to the Senior Associate Dean for Research 2004-2006 Committee member “UMMSM Imaging Core” (2004-2009) Committee member “UMMSM Mouse Genetics Core” (2004-present) Committee member “Office for Postdoctoral Affairs” (2006-2016) Committee member “MD/Ph.D steering committee” (2007-2017) Committee member “IBS admissions committee” (Neuroscience Alternate, 2005-present) Steering Committee member “Research Pillar, Dept. of Neurology” Search committee co-chair “Walter Bradley Chair in ALS Research” (Dept. of Neurology, 2009). Review Group Interdisciplinary Research Development Initiative (2008) Promotions and Tenure Committee (UMMSM- 2015-Present) Committee member “Neuroscience Program steering committee” (2016-present) Committee member “U-LINK Action Team meeting” (2017-2018) Committee member “Core Advisory committee” (2017-present) Ph.D. Thesis Committees (not as Mentor; past and present. Underlined-served as Chair)- George Brittan (Neuro/UMSM), Denise Abulafia (MCDB/UMSM), Enrique Cepero (MI/UMSM), Janet Talbot (Phys/UMSM), Yuhong Zuo (BMB/UMSM), Patrick Singleton (MCDB/UMSM), Craig Roberts (Neuro/UMSM), Radia Forteza (MCDB/UMSM), Jonathan Maher (MCDB/UMSM), Shanon Mendez (Neuro/UMSM), Carlos Arana (MCP/UMMSM), Amy Hower (Neuro/UMMSM), Nyguyen Khamh (Neuro/UMMSM), Neal Satterly (MCP/UMMSM), Darrell Horn (BMB/UMMSM), Iliana Soto (BMB/UMMSM), Christie Taylor (BMB/UMMSM), Mathew Brentall (CAB/UMMSM), Stephanie Glass (Neuro/UMMSM), David Barakat (MCDB/UMMSM), Donald S. McCorquodale (Neuro/UMMSM), Kahlilia Morris (Neuro/UMMSM), Haibin Xi (CAB/UMMSM), Rui Zhan (MCDB/UMMSM), Margaret Benny (MCDB/UMMSM), Charles Cohan (Neuro/UMMSM), Katia K. Oliveira (Master’s thesis committee, Universidade Federal de São Paulo, Brazil), Alexander Keymerman (Neuro/UMMSM), Keryn Hughes (BMB/UMMSM), Han Gao (Neuro/UMMSM), Alleene Strickland (Neuro/UMMSM), Dawn Owens (MCDB/UMMSM), Kevin Koronowski (Neuro/UMMSM), Ya-Ting Tu (BMB/UMMSM), Dasmanthie De Silva (BMB/UMMSM), Austin Choi (Neuro/UMMSM), Andrea Ruetenik (Neuro/UMMSM), Jonathan Weitz (MCDB/UMMSM), Hyun-Jung Kim (BMB/UMMSM), Hui Zhong (BMB/UMMSM), Nathalie Khoury (Neuro/UMMSM), Melanie Plastini (Neuro/UMMSM), Jing Xu (Neuro/UMMSM). Henna Tyynismaa, Opponent on Ph.D. defense (University of Helsinki-10/07) Amy Vincent, External Examiner on PhD defense (University of Newcastle-3/17)
31. Community Activities:
Curriculum Vitae Carlos T. Moraes 37 Mentor. American Heart Association Summer High School Research Program (96-97) Mentor. Dade County High School Advanced Research Internship Program (96-97)