OXFAM-DELAGUA

Water analysis kit

OXFAM-DELAGUA water analysis kitWATSAN M15 ERUWATSAN M15 ERU 2

Contents

1. Components2. Capabilities3. Maintenance4. Summary of bacteriological analysis process

Título de la presentación

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1. Componentes1. Components

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– Filtration apparatus– Incubator– Culture Medium– Petri Dishes– Sampling Methods– Turbidimeter– pH and Chlorine meters – Consumables

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Filtration apparatus

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Spares case

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External battery

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2. Capabilities

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CAPABILITIES

• Turbidity

• pH

• Free and total chlorine

• Bacteriological analysis (thermotolerant faecal coliforms)

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3. Maintenace

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GENERAL MAINTENANCE

Weekly

• Wash, rinse and dry the filtration apparatus

• Apply a smear of silicone grease to the black rubber O-ring

• Charge the internal battery fully at the end of each week

CAREFULLY READ THE MANUAL

Monthly

• Check the incubator temperature and recalibrate if necessary

• Check all the kit components

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BATTERY

• Never allow the internal battery to discharge fully.

• Never leave the incubator switched on for more than 18 consecutive hours

• Always recharge the battery at the end of the working period in the field.

• Recharge monthly during storage.

CAREFULLY READ THE MANUAL

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4. Summary of the bacteriological analysis process

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• Preparation:– Sterilization of equipment: (autoclave, pressure

cooker, methanol, boiling)– Petri: 15 min in autoclave or with methanol– Filtration apparatus: 15 min with methanol

– Culture medium (38 gr/500 ml distilled water)

– Petri dishes (Up to 16 daily samples)

• Filtration processFiltration volume (10 ml, 50 ml,100 ml) Filtration control

• Incubation (16-18 H 44ºC)

• Counting colonies: Yellow colour (faecal coliforms)

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• Presentation of resultsThe results are presented as CFU/100mlCFU (Colony forming units)

• 100 ml of sample (treated or very clean water) have been filtered: CFU/100ml = nº of identified colonies as coliforms• 50 ml of sample have been filtered: CFU/100ml = nº of identified colonies as coliforms x 2• 10 ml of sample have been filtered: CFU/100ml = nº of identified colonies as coliforms x 10

It is possible to use smaller volumes in samples with higher contamination.

The results should be calculated multiplying by the correspondent dilution factor.

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Thank youThank you……….……….