INSTITUTE OF HEALTH TECHNOLOGY, DHAKADepartment of Laboratory Medicine

BSc in Health Technology (Laboratory)- 1st Year

MYCOLOGYLecture No. 7.2 (Opportunistic Mycoses -Rhinosporidiosis)

By

Sk. MIZANUR RAHMANLecturer, Mycology

MS in Biotechnology & Genetic Engineering (UODA)MS in Microbiology (SU)

Rhinosporidiosis

Sites: nasal, oral, (palate, epiglottis), conjunctiva

Lesion: polyps, papilomas, warts-like lesion

More seen in communities near swamps

Etiology: Rhinosporidium seeberi

Laboratory diagnosis:

specimens, biopsy tissue

Direct microscopy: stained section or smears KOH, will show spherules with endospores

Culture on SDA will be negative

• Rhinosporidiosis is an infection of themucocutaneous tissue caused by Rhinosporidiumseeberi, an as yet unisolated and unclassifiedfungus.• It causes a chronic granulomatous diseasecharacterised by the production of large polyps,tumours, papillomas, or wart-like lesions. Thenose is the most commonly affected site.

Rhinosporidiosis.

Rhinosporidiosis.

• 1st identified in Argentina, but majority of cases

occur in India and Sri lanka.

• High incidence among people who frequently

bath along with domestic animals in ponds,

tanks, lakes

Clinical Features

• Chronic granulomatous disease of mucous

membrane.

• Characterised by the development of friable

polyps in the nose, mouth or eye.

• Miscellaneous forms –

Buccal cavity,vagina,

vulva, penis, urethra

or rectum

Laboratory Diagnosis• Cannot be cultured

Direct Examination• FNAC, Biopsy of lesion, Nasal washing

- Contains sporangia filled with thousands of sporangiospores(6-9µ) embedded in a stromaof connective tissue & capillaries

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432

Rhinosporidium seeberi

• Title: Rhinosporidiosis

• Disease(s): Rhinosporidiosis

• Legend: Rhinosporidiosis. Sporangia in a human polyp, GMS stain.

• Genus/Species: Rhinosporidium seeberi

• Image Type: Histopathology