NEUROBIOLOGY IMAGING FACILITY - Harvard University · o Slides, culture dishes, chambers and plates...

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Acquire Visualize and Analyze o Live or fixed o Immunolabeled and histological samples o Slides, culture dishes, chambers and plates o Single molecule to whole animal imaging o Micro-second to multiday experiments o Fluorescence, transmission, spectral unmixing, Ca+ imaging, photoconversion, photoactivation, DIC, MPE Whole organ tiling at high resolution by confocal or widefield Spectral Imaging STORM Super resolution microscopy with multiplexing Fiji with .vsi reader Metamorph with quick analysis modules Dedicated staff to help you with your imaging needs whether you are an imaging expert or have never used a microscope before. Acquire stunning images from whole organ to single molecule Visualize and analyze your samples with workstations you can VPN into from anywhere o Training and advice to fit your needs o Online reservations for all systems o Trained users can access the equipment 24hrs, 365 days o Help with experiment approach and sample prep o Support and advanced trainings when you need it o Online reservation and signup o Users can access equipment 24 hours a day 365 days/year o Offer full service imaging to fit your busy schedule Access Find Out More o Multi-channel cell scoring o neuron tracing, o particle measurements o volume measurements o tracking Corporate Affiliates Contact Info Imaging Overlord: Michelle Ocana [email protected] 617-432-1683 Science guy: Aurelien Begue [email protected] Imaging Technicians: Mahmoud el-Rifai [email protected] Michael Blanchard [email protected] Admin Assistant: Celia Muto [email protected] Laser Capture, Microdissec tion & Laser Tweezers CellProfiler Cellsens NEUROBIOLOGY IMAGING FACILITY 3D STED Super resolution Andor Spinning disk Lavision Lightsheet Macro Dissection Leica SPE confocal Everyone is welcome! No restrictions on core facility usage. NINDS grant holders enjoy subsidized rates. Multi- dimensional 3D Acquisition by confocal Zeiss PALM Zeiss LSM 510 Olympus FV1000 Whole Slide scanner Leica SP8X STED Widefield STORM Lightsheet imaging on whole animal or intact organ Fluorescent Recovery after Photobleach Calcium Imaging Amira Filament Tracing and Large Volume Module Bitplane Imaris Whole animal tiling at high resolution by widefield Huygens Scientific Volume Imaging

Transcript of NEUROBIOLOGY IMAGING FACILITY - Harvard University · o Slides, culture dishes, chambers and plates...

Page 1: NEUROBIOLOGY IMAGING FACILITY - Harvard University · o Slides, culture dishes, chambers and plates o Single molecule to whole animal imaging o Micro-second to multiday experiments

Acquire Visualize and Analyze

o Live or fixed

o Immunolabeled and histological samples

o Slides, culture dishes, chambers and plates

o Single molecule to whole animal imaging

o Micro-second to multiday experiments

o Fluorescence, transmission, spectral unmixing, Ca+ imaging,

photoconversion, photoactivation, DIC, MPE

Whole

organ tiling

at high

resolution

by confocal

or widefield

Spectral

Imaging

STORM

Super

resolution

microscopy

with

multiplexing

Fiji with .vsi reader

Metamorph with quick

analysis modules

Dedicated staff to help you with your imaging needs

whether you are an imaging expert or have never used a

microscope before.

Acquire stunning images from whole

organ to single moleculeVisualize and analyze your samples with

workstations you can VPN into from

anywhere

o Training and advice to fit your needs

o Online reservations for all systems

o Trained users can access the equipment 24hrs, 365 days

o Help with experiment approach and sample prep

o Support and advanced trainings when you need it

o Online reservation and signup

o Users can access equipment 24 hours a day 365 days/year

o Offer full service imaging to fit your busy schedule

Access

Find Out More

o Multi-channel cell scoring

o neuron tracing,

o particle measurements

o volume measurements

o tracking

Corporate Affiliates

Contact InfoImaging Overlord: Michelle Ocana

[email protected]

617-432-1683

Science guy: Aurelien Begue

[email protected]

Imaging Technicians: Mahmoud el-Rifai

[email protected]

Michael Blanchard

[email protected]

Admin Assistant: Celia Muto

[email protected]

Laser

Capture,

Microdissec

tion & Laser

Tweezers

CellProfiler

Cellsens

NEUROBIOLOGY IMAGING FACILITY

3D STED

Super

resolution

Andor Spinning disk Lavision Lightsheet Macro Dissection Leica SPE confocal

Everyone is welcome! No restrictions on core facility

usage.

NINDS grant holders enjoy subsidized rates.

Multi-

dimensional

3D

Acquisition

by confocal

Zeiss PALM Zeiss LSM 510 Olympus FV1000 Whole Slide scanner Leica SP8X STED Widefield STORM

Lightsheet

imaging on

whole animal

or intact organ

Fluorescent

Recovery

after

Photobleach

Calcium

Imaging

Amira Filament Tracing

and Large Volume Module

Bitplane Imaris

Whole

animal

tiling at

high

resolution

by

widefield

Huygens Scientific

Volume Imaging