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WBC Count

Specific Equipment1. White pipette2. Rubber pipe3. Plastic syringe4. Pipette vibrator5. Hemacytometer & cover slip (Counting chamber)6. Microscope7. Counter (hand tally)8. Report form

WBC Count

Reagent WBC diluting fluids 1. Turk’s solution Glacial acetic acid Gential violet Distilled water 2. Two % acetic acid 3. One % hydrochloric acid

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Specimens1. Fresh blood2. Anticoagulated blood

EDTADouble OxalateCitrateHeparinEtc.

WBC Count

WBC Count

Procedure 1. Mix the blood sample

thoroughly. 2. Fill blood into white pipette at

0.5 mark. 3. Fill reagent add up into the

pipette to 11 mark. 4. Shake the pipette on the

vibrator for 1 min. 5. Discarded the first 3-4 drops. 6. Fill in the hemacytometer

nicely. 7. Allow WBC to set down for 2-3

min. 8. Count 4 white squares under

microscope 9. Calculate the WBC

concentration.

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R R

R R

W W

W W

1 mm.

3 mm.

1 mm.

1 mm.

R

High 0.1 mm.

WBC Count

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W

1 mm.

1 mm.

High 0.1 mm.

WBC Count

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WBC Count Calculation1. Volume of 1 white square = 1 x 1 x 0.1 cu.mm.

= 0.1 cu.mm.2. Volume of 4 white square = 0.1 x 4 cu.mm.

= 0.4 cu.mm.3. In 0.4 cu.mm. the WBC count = N (counted No.)4. In 1 cu.mm. the WBC count = N x 1 / 0.4

= N x 2.55. The dilution for WBC = 0.5 / 10

= 206. The final WBC count = N x 2.5 x 20

= 50 N (/cu.mm.)

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WBC Count Calculation

White cell count = number of cells counted (N)

x volume factor (=2.5)

x dilution factor (=20)

= N x 50

Normal range = 5,000 - 10,000 / cu.mm.

5 - 10 x 103 / cu.mm.