LYCOPENE EXTRACTION FROM TOMATOES

ANA ÁLVAREZ MARTÍN LAURA HERNANDEZ SALAMANQUES

CONTENT

• Introduction• Extraction using a solvent• Supercritical extraction• Extraction assisted by enzyme

LYCOPENE

• Type of carotenoid• Aditive, colorant and

antioxidant• Tomato pomace• Ripe tomatoes

CONTENT

• Introduction• Extraction using a solvent• Supercritical extraction• Extraction assisted by enzyme

EXTRACTION USING A SOLVENT

• Fat-soluble: extracted with organic solvents• Raw material: tomato waste skin• Solvent:

hexane:acetone:alcohol (2:1:1) + BHT.

• Variables

EXTRACTION USING A SOLVENT VARIABLES

Number of extractionSolvent/meal ratio

Solvent/meal ratioParticle size

EXTRACTION USING A SOLVENT VARIABLES

TemperatureNumber of extractions

Particle sizeNumber of extractions

EXTRACTION USING A SOLVENT VARIABLES

Extraction timeNumber of extractions

EXTRACTION USING A SOLVENT OPTIMUS CONDITIONS

solvent/meal: 30:1 v/w4 extractions50 °Cparticle size 0.15 mmextraction time 8 min. Lycopene yield: 1.99 mg/100 g

CONTENT

• Introduction• Extraction using a solvent• Supercritical extraction• Extraction assisted by enzyme

SUPERCRITICAL

• Supercritical fluid extraction (SFE)– Tc, Pc solvent– CO2: • low critical temperature, • not toxic, • not flammable, • low cost ,• high purity

SUPERCRITICAL

• Semi-continuos process• Equipment: an extractor, a back pressure

regulator, a chiller, a high pump for CO2, collection vials and a wet gas meter.

• Optimization: tomato seed oil (co-solvent)

SUPERCRITICAL

Constant flow rate:

SUPERCRITICAL

Constant pressure:

SUPERCRITICAL

Constant temperature:

SUPERCRITICAL

• OTHER FACTORS:– Rising method– Tomato peel to seed ratio– Particle size– Degradation lycopene

CONTENT

• Introduction• Extraction using a solvent• Supercritical extraction• Extraction assisted by enzyme

EXTRACTION ASSISTED BY ENZYME

• Improve lycopene extraction• Response surface methodology (RSM)• Chemicals:– Acetona, ethanol, hexane– BHT

• Enzimes:– Peclyve PR– Cellulyve 50LC

EXTRACTION ASSISTED BY ENZYME

• Optimal conditions:– T=30ºC– Extraction time=3.18h– Enzyme load=0.16kg/kg– Enzyme preparation= 50:50 Pec-Cel