E Wild -type of 35S:MYB106-SRDX F flowers and torenia leaf. · PDF fileSupplemental Data....

Supplemental Data. Oshima et al. (2013). Plant Cell 10.1105/tpc.113.110783

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Supplemental Figure 1. Cuticle permeability

of 35S:MYB106-SRDX Arabidopsis flowers

and torenia leaf.

(A) Wild type stained with Toluidine Blue (TB).

No staining was observed.

(B) Weak-phenotype line of

35S:MYB106-SRDX stained with TB. The tops

and margins of sepals were stained; the anther

was also stained.

(C) and (D) Siliques grew on wild-type plants

but did not grow on 35S:MYB106-SRDX.

(E) and (F) Wild-type torenia (E) and 35S:MYB106-SRDX torenia (F), stained with TB.

Bars indicate 1 mm in (A) and (B) and 2 mm in

(E) and (F).

Wild-type 35S:MYB106-SRDX

A B

Wild-type 35S:MYB106-SRDX (mild)

C

D

35S:MYB106-

SRDX (torenia)

Wild-type

(torenia)E F


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A

B


3 / 18

Supplemental Figure 2. Expression and motif analyses based on public

microarray data or conserved motifs among MYB subgroup 9 genes/proteins,

respectively.

(A) Comparison of microarray data of MYB106, MYB16 and MYB17 along with

developmental stages using AtGenExpress Visualization Tool

(http://jsp.weigelworld.org/expviz/expviz.jsp). X and Y axes represent different

developmental stages or signal intensity, respectively.

(B) One-by-one comparison of microarray data of MYB106, MYB16 and MYB17

along with developmental stages using ATTED-II database (Obayashi et al., 2009).

Top-left, top-right and bottom panels represent comparison of MYB16 (X axis) and

MYB17 (Y axis), MYB106 (X axis) and MYB17 (Y axis), and MYB16 (X axis) and

MYB106 (Y axis), respectively. Each axis represents signal intensity.

(C) Comparison of conserved protein motifs among MYB subgroup 9 proteins

collected from various species. The analysis was performed by SALAD database

(Mihara et al., 2008). At, Arabidopsis thaliana; ARALYDRAFT, Arabidopsis lyrata; Gt,

Gentiana triflora; VIT, Vitis vinifera; MTR, Medicago truncatula; Am, Antirrhinum

majus; Cs, Cucumis sativus; Pt, Populus trichocarpa; Gm, Glycine max; Dac,

Daucus carota; Tf, Torenia fournieri; Pf, Perilla frutescens; RCOM, Ricinus

communis; La, Lupinus albus; Md, Malus x domestica; Rh, Rosa hybrid cultivar; If,

C M YB 106

M YB 16

M YB 17

M YB 106 /

M YB 16

group

M YB 17

group

Md_MYB13

At_MYB106

Am_M IXTA

Pt_MYB138

Pt_MYB038

Am_MYBML1

Am_MYBML3

Hi_M IXTA1

Am_MYBML2

Ah_MYB25

Dac _MYB2

At_MYB16

Pt_MYB083

Dec _MYBML1

Ph_MYB1

Zm_MYB34

Md_MYB14

At_MYB17

Tf_MYBML3

Pt_MYB187

Pt_MYB077

C


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Iris fulva; Zm, Zea mays; SORBIDRAFT, Sorghum bicolor; Os, Oryza sativa

Japonica Group; PHYPADRAFT, Physcomitrella patens; Bd, Brachypodium

distachyon; Ta, Triticum aestivum; Hv, Hordeum vulgare; Dec, Dendrobium

crumenatum; Gh, Gossypium hirsutum; Hi, Humulus lupulus; Ph, Petunia x hybrid;

AmMIXTA, CAA55725; Am-MYBML1, CAB43399; Am-MYBML2, AAV70655;

Ph-MYB1, CAA78386; Pt-MYB083, XP_002323871; Dc-MYB2, BAF49441;

Pt-MYB187, POPTRDRAFT_572225; Md-MYB14, AAZ20433; Pt-MYB077,

POPTRDRAFT_575814; Zm-MYB34, AAL90652; Md-MYB13, AAZ20432;

Dec-MYBML1, ADD64500; Gh-MYB25, ACJ07153; Pt-MYB038,

POPTRDRAFT_567011; Pt-MYB138, POPTRDRAFT_564238; Hi-MIXTA1,

BAF46264.


5 / 18

Supplemental Figure 3. Phenotypes

caused by constitutive expression of

MYB16-SRDX.

(A) Fused leaves of 4-week-old

35S:MYB16-SRDX plant.

(B) Scanning electron micrographs of fused

flower of 35S:MYB16-SRDX plants.

(C) Surface of stem of 35S:MYB16-SRDX

plant. Cuticular wax was reduced.

Bars indicate 5 mm in (A), 0.2 mm in (B),

33.33 μm in (C).


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M

YB

16

pro

:GU

S

I

I

J

G H

MY

B1

06

pro

:GU

S A B C

D

F

E

Supplemental Figure 4. Promoter activities of MYB106 and MYB16. (A) to (E) GUS reporter activity (blue color) driven by the promoter of MYB106 in 3-week-old plants (A), trichomes (B), stem and pedicel (C), stem (D), and dehiscence zone between carpel and pedicel (E). (F) to (J) GUS reporter activity driven by the promoter of MYB16 in 3-week-old plants (F), a young rosette leaf (G), stem (H), epidermis of rosette leaf (I) and mesophyll cell (J). Bars indicate 0.1 mm in (B) to (E) and (G) to (J), 2 mm in (A) and (F).


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Supplemental Figure 5.

Promoter activities of

MYB106 and MYB16 in

floral organs.

GUS reporter activity (blue color) driven by the promoter of MYB106 or MYB16 in buds and flower. Asterisks and circles indicate petals and stamens showing GUS activity, respectively. Bars indicate 0.1 mm.

MYB16pro:GUSMYB106pro:GUS

Stage 10

Stage 11

Stage 12

early

Stage 12

late

Stage 13

*

*

**

*

*

*

* *

*

*

*

*

*

○

○

○

○

○

○

○

○

○

○

○

○

○

○


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35S:MYB106 -RNAi

A Seedlings Buds B

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

Rela

tive a

mo

un

t o

f M

YB

106 m

RN

A

*

**

35S:MYB106-RNAi

D

D

35S:MYB106-RNAi

0

0.2

0.4

0.6

0.8

1

1.2

1.4

Rela

tive a

mo

un

t o

f M

YB

16 m

RN

A

*

Seedlings Buds

C E


Plants with reduced levels

of MIXTA-like MYBs.

(A) and (B) Quantitative

RT-PCR analysis of 3’

non-coding region of MYB106

(A) and coding region of

MYB16 (B) in 3-week-old

plants (n = 4 to 6) and buds of

35S:MYB106RNAi and

wild-type plants (n = 4 to 6).

Expression of MYB106 and

MYB16 was reduced in the

RNAi line. Error bar represent

standard deviation. Single

asterisks indicate P < 0.05 in

Welch’s t-test. (C) Rosette leaf

and magnified view of

trichome on it of

35S:MYB106-RNAi

Arabidopsis stained with TB.

(D) Flower of

35S:MYB106-RNAi plants

stained by TB. Organ

adhesion occurs inside flower.

Edge of sepal and petal is

stained by TB. (E) Entire

35S:MYB106-RNAi plants,

which are almost sterile due to

organ adhesion inside the

flower. Bars indicate 100μm in

(C), 1mm in (D).


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Constitutive expression of

SHN3-SRDX caused reduction of

cuticular wax with severe

morphological changes.

(A) Fused leaves of 3-week-old

35S:SHN3-SRDX Arabidopsis.

(B) Fused leaves and buds of

10-week-old 35S:SHN3-SRDX plant.

(C) to (E) Surface of stem (C) and

petal (E) of 35S:SHN3-SRDX plant

observed by SEM. Fewer wax

crystals and cutin nanoridges than

wild type (D) were observed.

(F) TB uptake per gram protein.

Error bars represent standard error

(n=8). Double asterisks represent

P<0.01 by Welch’s t-test. (G) Leaf of

35S:WIN1-SRDX torenia stained

with TB. Bars indicate 5 mm in (A)

and (B) and 2 mm in (G) 33.33 μm in

(C), 10 μm in (D) and (E).

35S:SHN3-SRDXA

B

35S:SHN3-SRDX

(Petal)

Wild-type

(Petal)D

E

35S:SHN3-SRDX

C 35S:SHN3-SRDX(Stem)

10ul

0.00

0.02

0.04

0.06

0.08

0.10

TB

/pro

tein

(g/g

) **F G 35S:WIN1-SRDX

(torenia)


10 / 18

Supplemental Table 1. Frequency of observed phenotypes for four transgenes

Severe Intermediate Mild Wild-type like Total

35S:MYB106-SRDX 7 7 16 1 31

35S:WIN1-SRDX 17 34 1 13 65

MYB106pro:MYB106-SRDX 0 9 7 12 28

35S:MYB106-RNAi 0 14 24 8 46

Severe, intermediate, mild phenotypes represent organ adhesions in whole plant, immature

trichome and organ adhesion in limited tissues, over-branched trichome, respectively. “Total”

represents the number of plants examined for each transgene.


11 / 18

Supplemental Table 2. Branch numbers of leaf trichomes

Branch number of trichome Total*

1 2 3 4 5 6 7 8

Wild-type 0.60 7.23 90.06 2.11 0.00 0.00 0.00 0.00 664

35S:MYB106-SRDX 5.99 22.12 53.92 13.36 4.61 0.00 0.00 0.00 217

MYB106pro:MYB106-SRDX 98.59 1.41 0.00 0.00 0.00 0.00 0.00 0.00 142

myb106-1 0.47 10.54 59.95 23.65 5.39 0.00 0.00 0.00 427

myb106-2 2.03 18.06 38.60 23.02 17.38 0.90 0.00 0.00 443

MYB16amiRNA 0.25 2.78 81.27 15.44 0.25 0.00 0.00 0.00 395

MYB16amiRNA myb106-2 0.23 0.45 9.01 15.09 50.00 20.50 4.50 0.45 444

Numbers in table are percentage of trichomes with indicated branch number.

*Total number of trichomes on leaf 3 and 4 of five plants.


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Supplemental Table 3. Down regulated gene groups in both MYB106-SRDX and

WIN1-SRDX plants

GO term Number

of

genes a

Downregulated

genes b

P-value c Odds

ratio d

Cuticle development 45 15 3.63E-11 10.5386

Wax biosynthetic process 16 3 0.01482 5.92795

Cutin biosynthetic process 10 5 2.01E-05 15.8079

Very-long-chain fatty acid

metabolic process

43 16 1.62E-12 11.764

Fatty acid elongase activity 6 4 4.62E-05 21.0772

Carboxylesterase activity 129 14 8.86E-05 3.43117

Lipid metabolic process 246 21 5.81E-05 2.69891

Transferase activity,

transferring acyl groups other

than amino-acyl groups

73 10 0.0001437 4.33092

Lipid transport 157 13 0.001836 2.61787

Hydrolase activity, acting on

ester bonds

172 13 0.003944 2.38956

Secondary cell wall biogenesis 35 6 0.0009878 5.41984

Plant-type cell wall

modification involved in

multidimensional cell growth

25 3 0.04602 3.79389

Response to auxin stimulus 262 34 3.91E-08 2.96944


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a Number of genes included in each GO category

b Number of genes downregulated in both 35S:MYB106-SRDX and 35S:WIN1-SRDX

plants c P-value of Fisher’s exact test

d Ratio between actually observed number and expected number by chance


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Supplemental Table 4. Changes in expression of cuticle related genes by microarray analysis.Locus Common name Short description #1 #2 #3 #4AT4G34520 KCS18, FAE1 3-ketoacyl-CoA synthase 18 1.01 0.95 0.83 0.73

AT1G62340 ALE1 PA-domain containing subtilase family protein 1 0.95 0.94 0.68

AT1G49430 LRD2, LACS2 long-chain acyl-CoA synthetase 2 0.37 0.2 3.04 1.96

AT1G01610 ATGPAT4, GPAT4 glycerol-3-phosphate acyltransferase 4 0.35 0.12 3.31 1.46

AT1G36160 GSD1, PAS3, GK, EMB22, AT-ACC1, ACC1 acetyl-CoA carboxylase 1 0.62 0.42 1.2 1.45

AT1G68530 KCS6, CER6, POP1, G2, CUT1 3-ketoacyl-CoA synthase 6 0.62 0.31 1.55 1.88

AT1G02205 CER1 Fatty acid desaturase 0.24 0.08 2.79 3.33

AT3G10570 CYP77A6 cytochrome P450, family 77, subfamily A, polypeptide 6 0.39 0.45 10.9 14.63


AT2G04570 GDSL-like Lipase/Acylhydrolase superfamily protein 0.53 0.13 20.03 13.25


AT2G38110 ATGPAT6, GPAT6 glycerol-3-phosphate acyltransferase 6 0.21 0.18 2.87 5.52

AT5G23940 PEL3, DCR, EMB3009 HXXXD-type acyl-transferase family protein 0.44 0.64 1.38 1.87

AT5G10480 PEP, PAS2 Protein-tyrosine phosphatase-like, PTPLA 0.5 0.7 1.17 2.36

AT1G67730 ATKCR1, YBR159, KCR1 beta-ketoacyl reductase 1 0.45 0.84 1.02 1.43

AT4G33790 G7, FAR3, CER4 Jojoba acyl CoA reductase-related male sterility protein 0.04 0.44 1.32 4.67

AT1G10670 ACLA-1 ATP-citrate lyase A-1 0.63 1 1.08 0.95

AT4G34250 KCS16 3-ketoacyl-CoA synthase 16 0.12 0.1 0.12 0.95



AT4G28110 ATMYB41 myb domain protein 41 0.21 0.55 0.23 1.74

AT5G23190 CYP86B1 cytochrome P450, family 86, subfamily B, polypeptide 1 0.06 0.39 0.07 0.61


AT4G24510 VC2, VC-2, CER2 HXXXD-type acyl-transferase family protein 0.21 0.01 2.66 0.75

AT2G20300 ALE2 Protein kinase superfamily protein 0.71 0.66 1.07 0.8

AT1G25450 KCS5, CER60 3-ketoacyl-CoA synthase 5 0.18 0.16 1.31 1.02


AT4G00360 ATT1, CYP86A2 cytochrome P450, family 86, subfamily A, polypeptide 2 0.21 0.25 2.08 0.86

AT2G47240 CER8, LACS1 AMP-dependent synthetase and ligase family protein 0.32 0.35 0.68 2

AT1G51500 AtABCG12, WBC12, ABCG12, D3, CER5, ABC-2 type transporter family protein 0.47 0.2 0.74 1.63

AT2G34770 ATFAH1, FAH1 fatty acid hydroxylase 1 0.2 0.35 0.64 0.95

AT4G00400 AtGPAT8, GPAT8 glycerol-3-phosphate acyltransferase 8 0.05 0.1 0.99 0.73

AT2G45970 CYP86A8, LCR cytochrome P450, family 86, subfamily A, polypeptide 8 0.18 0.31 0.82 0.9

AT2G26250 KCS10, FDH 3-ketoacyl-CoA synthase 10 0.18 0.21 1.13 2.06

AT4G24140 BDG3 alpha/beta-Hydrolases superfamily protein 0.14 0.27 0.91 1.41

AT3G55360 ECR, CER10, ATTSC13, TSC13 3-oxo-5-alpha-steroid 4-dehydrogenase family protein 0.57 0.66 1.06 1.11



AT5G57800 CER3, FLP1, YRE, WAX2 Fatty acid hydroxylase superfamily 0.52 0.4 1.89 0.43

AT2G27690 CYP94C1 cytochrome P450, family 94, subfamily C, polypeptide 1 0.43 0.08 3.14 0.2

AT3G28910 AtMYB30, MYB30 myb domain protein 30 0.67 0.66 1.68 0.71

AT5G47120 ATBI-1, ATBI1 BAX inhibitor 1 1.22 0.97 1.18 1.35

AT3G54010 DEI1, PAS1 FKBP-type peptidyl-prolyl cis-trans isomerase family protein 1.02 1.11 1.2 1.27


AT1G69500 CYP704B1 cytochrome P450, family 704, subfamily B, polypeptide 1 1.25 4.46 1.43 2.12

AT1G57750 MAH1, CYP96A15 cytochrome P450, family 96, subfamily A, polypeptide 15 1.38 2.42 1.39 0.74

AT3G60500 G3, CER7 3'-5'-exoribonuclease family protein 1.47 1.54 1.12 0.9

AT2G33510 AtCFL1 unknown protein 1.51 1.81 0.68 0.94

AT4G34510 KCS17, KCS2 3-ketoacyl-CoA synthase 17 0.95 1.39 0.55 1.11

AT4G14070 AAE15 acyl-activating enzyme 15 1.34 1.06 0.64 1.1

AT3G12990 RRP45A ribonuclease PH45A 1.11 1.08 0.76 1.24

AT5G37300 WSD1 O-acyltransferase (WSD1-like) family protein 0.44 0.81 0.29 8.48

AT2G46720 KCS13, HIC 3-ketoacyl-CoA synthase 13 1.22 0.32 0.72 1.17

AT3G61150 HD-GL2-1, HDG1 homeodomain GLABROUS 1 1.46 0.56 0.69 1.5

#1 35S_MYB106SRDX_3wk_seedling/Col0_3wk_seedling

#2 35S_WIN1SRDX_3wk_seedling/Col0_3wk_seedling

#3 WIN1_ox_2wk_seedling/vector_control_2wk_seedling

#4 MYB106VP16_seedling2/Col0_seedling_Oshima

Cuticle related genes were collected by the Gene ontology terms as cuticle development, cutin biosynthetic process, wax biosynthetic process, alkane 1-

monooxygenase activity, very long-chain fatty acid biosynthetic process, fatty acid elongation, fatty acid elongation, very long-chain fatty acid

biosynthetic process and very long-chain fatty acid metabolic process besides AT2G45970 (Wellesen et al. 2001), AT2G04570 (Kannangara et al. 2007),

AT5G10480 (Bach et al. 2008), AT3G61150, AT2G33510(Wu et al. 2011), AT4G24140 (Kurdyukov et ol. 2006, Shi et al. 2011).


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Supplemental table 5. Phenotypes of loss-of-function plants

trichome petal cell outgrowth cuticle deficiency

nok-122 over-branching Folkers et al. 1997

myb106-1 over-branching,

immature

Guildinf and Marks 2010

Jakoby et al. 2008

petal and filament

nanoridge this study

myb106-2

over-branching,

less-branching,

immature

this study petal and filament


MYB16amiRNA over-branching this study filament nanoridge this study

MYB16amiRNA myb106-2 over-branching and

immature this study supressed this study

petal and filament


35S:MYB106-SRDX

over-branching,

less-branching,

immature

this study supressed this study stem, leaf, petal

nanoridge, trichome this study

35S:MYB16-SRDX stem this study

MYB106pro:MYB106-SRDX less-branching,

immature this study supressed this study

stem, petal, stamen,

carpel, trichome this study

Antirrhinum mixta supressed Noda et al. 1994

Petunia phmyb1 supressed Baumann et al. 2007

Supplemental table 6. Phenotypes of gain-of-function plants and references

trichome petal cell outgrowth ectopic formation of specific cuticle

MYB106-VP16 less-branching,

early maturation this study nanoridge on leaves this study

MYB106ox less-branching,

early maturation this study nanoridge on carpel this study

MYB16ox enhanced in tobacco Baumann et al. 2007

AmMYBML1ox ectopic formation in

tobacco

Rerez-Rodriguez et al.

2004

glandular trichome

formation in tobacco

Rerez-Rodriguez et al.

2004

AmMYBML2ox enhanced in tobacco Baumann et al. 2007

PhMYB1ox enhanced in tobacco Baumann et al. 2007

MIXTAox trichome formation in

tobacco Glover et al. 1998


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Supplemental Table 7. Primers used in this study.

Name Sequenses (5' - 3') Purpose

AT3G01140N gATGGGCAGATCGCCATGTTGTGATAAGGC 35S:MYB106SRDX, 35S:MYB106, 35S:MYB106VP16, MYB106pro:MYB106SRDX

AT3G01140C GAACATCGTCGCGGAATCGGACGGTGAAGA 35S:MYB106SRDX, 35S:MYB106VP16, MYB106pro:MYB106SRDX

AT1G15360F ATGGTACAGACGAAGAAGTTCAG 35S:WIN1SRDX, 35S:WIN1

AT1G15360R GTTTGTATTGAGAAGCTCCTCTATC 35S:WIN1SRDX

At5G25390F ATGGTACATTCGAAGAAGTTCCG 35S:SHN3SRDX

At5G25390R GACCTGTGCAATGGATCCAG 35S:SHN3SRDX

AT5G15310N gATGGGTAGATCACCGTGTTGTGACAAATT 35S:MYB16SRDX, MYB16pro:MYB16SRDX

AT5G15310C GAACATCGGTGAATCCGACGGTGAAGGATC 35S:MYB16SRDX, MYB16pro:MYB16SRDX

AT1G15360.1SC TTAGTTTGTATTGAGAAGCTCCTCTATCAT 35S:WIN1

AT3G01140.1SC TCAGAACATCGTCGCGGAATCGGACGGTGA 35S:MYB106

AT5G15310pFA GGCGCGCCAATTTGAGACACATAAACATCTAAGCA MYB16pro:MYB16SRDX

AT5G15310pRB CGCGGATCCTGTTTTGAGAGCAAAGAAATAAGA MYB16pro:MYB16SRDX

AT5G15310pF3000 GGGGACAACTTTGTATAGAAAAGTTGAATTTGAGACACATAAACATCTAAGCA MYB16pro:GUS, MYB16pro:LUC

AT5G15310pR GGGGACTGCTTTTTTGTACAAACTTGGCATTGTTTTGAGAGCAAAGAAATAAGA MYB16pro:GUS, MYB16pro:LUC

AT3G01140pFA GGCGCGCCTAATACTAAATTTATAAATGATGGAAA MYB106pro:MYB16SRDX

AT3G01140pRB CGCGGATCCTCAATTGGATGGGGTCGAGAGATA MYB106pro:MYB16SRDX

AT3G01140pF3000 GGGGACAACTTTGTATAGAAAAGTTGTAATACTAAATTTATAAATGATGGAAA MYB106pro:GUS, MYB106pro:LUC

AT3G01140pR2 GGGGACTGCTTTTTTGTACAAACTTGGCATTGTTTTCCCACACAAGTTATTAG MYB106pro:GUS, MYB106pro:LUC

AT1G15360pF3000 GGGGACAACTTTGTATAGAAAAGTTGTCTGAAGTCTTGTTTCTTCATTATTCT WIN1pro:GUS, WIN1pro:LUC

AT1G15360pR GGGGACTGCTTTTTTGTACAAACTTGGCATTCTTACTTACTCTGTGATGTTTGT WIN1pro:GUS, WIN1pro:LUC

AT1G01600pF2000 GGGGACAACTTTGTATAGAAAAGTTGCTTGTGTGTGGTCCGAAGAAGATGAGA CYP86A4pro:LUC

AT1G01600pR GGGGACTGCTTTTTTGTACAAACTTGGCATGTAGCTCTTTTATTATTGTTTCCC CYP86A4pro:LUC

Underlines indicate attB sequences. Double underlines indicate restriction enzyme recognition site.


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Supplemental Table 8. Primer sequences for RT-PCR.

Name Sequences (5' - 3') Purpose

FDHf TGCTGACGACCGTAGCTTCA quantitative RT-PCR for FDH

FDHr TTGTCTTGAGAGCTTCACCTCCAA quantitative RT-PCR for FDH

LCRf TGGTAACGGCGTTTCAAGTTAGG quantitative RT-PCR for LCR

LCRr GTTGGCCAAAGAACGGCGTATTTA quantitative RT-PCR for LCR

KCS1f GCGGTTTGGAAAGCGTTACG quantitative RT-PCR for KCS1

KCS1r CAATCGAACCAGCCCAAGCA quantitative RT-PCR for KCS1

CER2f CTTGTCTACGATCACGTTCTTGGT quantitative RT-PCR for CER2

CER2r TCCAAGAATATGGGCCCAACTCA quantitative RT-PCR for CER2

CER1f GTGAATGTCCACTCCTGTGAGA quantitative RT-PCR for CER1

CER1r AAGAAGGGATGTGCCACACTCA quantitative RT-PCR for CER1

LACS2f AAAGGCCACAGCTCCTCCAA quantitative RT-PCR for LACS2

LACS2r CCATGGACCTCTTTGCTTCTGA quantitative RT-PCR for LACS2

CYP86A4f GAAGAAGAGCGACGGTGTCT quantitative RT-PCR for CYP86A4

CYP86A4r TAAGTAAACGGCGACGCCTTCA quantitative RT-PCR for CYP86A4

CYP86A7f AGCTGGACGTGACACGTCAT quantitative RT-PCR for CYP86A7

CYP86A7r GACTCGTGGGTTAAGACTAACCA quantitative RT-PCR for CYP86A7

CYP77A6f GAGCAAGAGCTCTCGAGGTT quantitative RT-PCR for CYP77A6

CYP77A6r TCTCCGTCGTCTCTCGATGA quantitative RT-PCR for CYP77A6

2g04570f TCATCAAGAACCCTTCCTCCTTTG quantitative RT-PCR for AT2g04570

2g04570r GACAACCATATCCCATCTCGAACA quantitative RT-PCR for AT2g04570

MYB106f CAAACCAAGGAAACGGAGACCAA quantitative RT-PCR for MYB106

MYB106r CTGCTATCCGTAGGGATTCCTA quantitative RT-PCR for MYB106

MYB106-3'f ACCGTCCGATTCCGCGACGA quantitative RT-PCR for 3' noncoding region of

MYB106

MYB106-3'r GCAGGTTTGCTACATGCAATTCTGC quantitative RT-PCR for 3' noncoding region of

MYB106

WIN1/SHN1f AGCTCCTCCCAAGAGACTAC quantitative RT-PCR for WIN1/SHN1

WIN1/SHN1r TTTCAACTTCAGTGGTCGGAGCAA quantitative RT-PCR for WIN1/SHN1

MYB16f GATGGGTAGATCACCGTGTTGTGACA

AATT

quantitative RT-PCR for MYB16


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MYB16r GAACATCGGTGAATCCGACGGTGAAG

GATC

quantitative RT-PCR for MYB16

PP2AA3f GACCAAGTGAACCAGGTTATTGG RT-PCR for PP2AA3

PP2AA3r TACTCTCCAGTGCCTGTCTTCA RT-PCR for PP2AA3

Supplemental reference 1. Reference for Supplemental Figure 2B

Obayashi, T., Hayashi, S., Saeki, M., Ohta, H., and Kinoshita, K. (2009). ATTED-II

provides coexpressed gene networks for Arabidopsis. Nucleic Acids Res. 37, 987-991.

E Wild -type of 35S:MYB106-SRDX F flowers and torenia leaf. · PDF fileSupplemental Data....

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