Different biosensor strategies for clinical hepatitis B virus ......Different biosensor strategies...
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Different biosensor strategies for clinical hepatitis B virus diagnostic Hongyu YU, Ph.D. Department of Electrical and Electronic Engineering South University of Science and Technology of China
Transcript of Different biosensor strategies for clinical hepatitis B virus ......Different biosensor strategies...
Different biosensor strategies for clinical hepatitis B virus diagnostic
Hongyu YU, Ph.D.Department of Electrical and Electronic EngineeringSouth University of Science and Technology of China
An estimated 240 million people have chronic HBV infection globally.
Ott JJ, Stevens GA, et al. Vaccine. 2012;30(12):2212–2219.
Rapid and portable identification of hepatitis B virus has important implications for medical healthcare.
Traditional diagnostic methods: TIME CONSUMING, SPECIALIST LABORATORIES
PCR ELISA Cell Culture
Sensitivity (TCID50) 5-100 105 104
Biosensor’s advantages:
RapidHigh resolution and stabilityHigh performance, small & portabilityVery cheap and reproducibleNo need for sample preparation
Biosensor: Electrochemical sensor, Optical sensor, Piezoelectric sensor
Optical sensor--Surface plasmon resonance (SPR)
Electrochemical sensor--DNA sensorPiezoelectric sensor
Our strategies
Electrochemical DNA Sensor
Electrochemical Immunosensor
Surface Acoustic Waves Immunosensor
Higher resolution and stability;Simplicity in handling；High performance, small & portability；Real-time monitoring；Very cheap, sensor:$2/pcs, system:$15/pcs;Simultaneous detection of multiple analytes;Can be extended to wireless and passive App;
Our purpose : a POCT HBV diagnosis tool
Electrochemical Immunosensor
General overview of the main components
F. Ricci et al. / Electrochimica Acta 84 (2012)
Electrochemical DNA Sensor
Referenece: GenMark Diagnostics, Inc., https://www.genmarkdx.com
PCR required
HIGH SENSITIVITY, NO PCR
Thin-film Electrode for portability
Au
Target DNA
Auxiliary Probe
Signal ProbeCapture Probe
Au
Target DNA Auxiliary Probe
Capture Probe
Auxiliary Probe
MORE
Signal Probe
Au
Target DNA
Capture Probe
MORE
Target DNA
Target DNA
Signal Probe
Signal Probe
Signal Probe
Auxiliary Probe
Auxiliary Probe
Au
Capture Probe
MORE
Signal Probe
Signal Probe
Signal Probe
Target DNA
Chen, Xian, et al.Chem. Commun, 2011
Xia, Fan, et al.Journal of the
American Chemical Society, 2010
Our improvement
need for sample preparation
Surface Acoustic Waves Immunosensor
★SAW Biosensor’s Principle:An electrical signal is converted at the input transducer into surface acoustic waves, utilizing the piezoelectric properties of the substrate material. The waves are travelling across the sensitive area. Afterwards, the wave is converted back at the output transducer into an electrical signal. If there is molecular recognition and binding, a shift in the frequency and phase of the electrical signal is induced.
Länge, Kerstin, et al. Analytical and
bioanalytical chemistry 2008
(1) in which the sensor is immersed. The elements of the SAW biosensor are a piezoelectric crystal (2), IDTs (3), the surface acoustic wave (4), and immobilized antibodies (5) corresponding to the analyte molecules (6) in the sample. The driving electronics (7) operate the SAW biosensor and generate changes in the output signal (8) as the analytebinds to the sensor surface
Interdigital Transducer (IDT)
structure Frequency response
hepatitis B surface antibody (HBsAb) detection：hepatitis B surface antigen (HBsAg) was immobilized on the sensing area. which was covered with. Phase shift was monitored to detect specific binding of HBsAb.
To determine optimal design parameters, numerical simulation has been performed.
Our Chip
The experiment and the electrical systems including microfluidic channel.
Our future work
Improve sensitivity
Point-of-care testing (bedside testing)
Real-time monitoring
Simplicity in handling
Very cheap
