Cellulase enzyme
description
Transcript of Cellulase enzyme
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Cellulase enzyme
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EC Code =3.2.1.4Other name s: Avicelase- BETA-1,4-Endoglucan
Hydrolase - BETA-1,4Glucanase- Cello dextrinase Carboxy methyl cellulase ENDO-1,4-BETA-D-Glucanase-ENDO-1,4-BETA-D-Glucanohydrolase- ENDO-1,4-BETA-Glucanase- Endo glucanase -Alkali cellulase- Pancellasess
Systematic name:1,4-(1,3:1,4)-beta-D-glucan,4-glucano hydrolase
Introduction
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1-4-BETA-GLUCOSIDIC bond hydrolysis in cellulose,…
REACTION
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Endo-cellulaseExo-cellulasebeta-glucosidaseOxidative cellulaseCellulose phosphorylase
cellulases
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BacteriaFungiActinomyceteProtozoaSome insects that digest woodSome animals & plantsNot mammal
Cellulase sources
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DialysisFiltration gelAnionic resin exchange columnUsing aceton
Extraction
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Cellulase production
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Spectorophotometeryi. Reduced sugarii. Substrate decrease
Solid surface i. Propagation in substrateii. Electrophoreses gel
Cellulase activity assay
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Food industryAgricultural industryPaper making industryWaste water treatmentTextile industry
Cellulase application
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Enzymic hydrolysis rate
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Cellulase action mechanism
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Cellulase structure
The crystal structure of the catalytic domain of CelCCA
β strands and * helices in CelCCA.
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Topological diagram of the secondary structural elements of CelCCA. helices are shown as cylinders and β strands as arrows.
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Close up view of catalitic C-terminal extremlybof the barrel strands showing the arrangment of the 7 residues(red and yellow) along with the try (purple) at the intrancethe catalitic residues are red
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Thank for your patience