Cellulase enzyme
EC Code =3.2.1.4Other name s: Avicelase- BETA-1,4-Endoglucan
Hydrolase - BETA-1,4Glucanase- Cello dextrinase Carboxy methyl cellulase ENDO-1,4-BETA-D-Glucanase-ENDO-1,4-BETA-D-Glucanohydrolase- ENDO-1,4-BETA-Glucanase- Endo glucanase -Alkali cellulase- Pancellasess
Systematic name:1,4-(1,3:1,4)-beta-D-glucan,4-glucano hydrolase
Introduction
1-4-BETA-GLUCOSIDIC bond hydrolysis in cellulose,…
REACTION
Endo-cellulaseExo-cellulasebeta-glucosidaseOxidative cellulaseCellulose phosphorylase
cellulases
BacteriaFungiActinomyceteProtozoaSome insects that digest woodSome animals & plantsNot mammal
Cellulase sources
DialysisFiltration gelAnionic resin exchange columnUsing aceton
Extraction
Cellulase production
Spectorophotometeryi. Reduced sugarii. Substrate decrease
Solid surface i. Propagation in substrateii. Electrophoreses gel
Cellulase activity assay
Food industryAgricultural industryPaper making industryWaste water treatmentTextile industry
Cellulase application
Enzymic hydrolysis rate
Cellulase action mechanism
Cellulase structure
The crystal structure of the catalytic domain of CelCCA
β strands and * helices in CelCCA.
Topological diagram of the secondary structural elements of CelCCA. helices are shown as cylinders and β strands as arrows.
Close up view of catalitic C-terminal extremlybof the barrel strands showing the arrangment of the 7 residues(red and yellow) along with the try (purple) at the intrancethe catalitic residues are red
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