C146-E352 LCMSMS Method Package for Aminoglycoside Antibiotics Method... · Aminoglycosides are...

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LC/MS/MS Method Package for Aminoglycoside Antibiotics For LabSolutions LCMS Software Complete Solution for Quantitation and Confirmation of Aminoglycosides in Meat, Eggs and Milk Aminoglycosides are an antibiotic family widely used for the treatment of bacterial infections in cattle, sheep, pigs and poultry. Regulatory agencies have set maximum residue limits (MRL) for these compounds after veterinary use. Aminoglycosides are very polar compounds, and usually require the use of undesirable ion-pairing reagents with LC-MS/MS. This Method Package is a complete solution for the simultaneous quantitative analysis of all regulated aminoglycosides by HILIC/MS/MS. It includes: A sample preparation protocol using mixed-mode weak cation exchange from Biotage. This protocol produces high recoveries and low matrix effects, and is adapted to meat, milk or egg samples. A primary method for fast (< 5 min) quantitative analysis by HILIC/MS/MS with two MRMs per compound. A second method for confirmation of identity. Using the same mobile phases and column, the gradient is modified and 15 optimized MRMs are acquired per compound. This enables library searching and accurate identification on Insight Screening using MRM Spectrum Mode (library provided). LCMS-8060 C146-E352 Complete method for fast quantitative screening of 13 aminoglycosides in meat, eggs and milk. Increase identification confidence with MRM Spectrum mode and LabSolutions Insight Screening. No ion-pairing reagent required. Includes single sample preparation protocol usable for meat, eggs and milk. Amikacin Apramycin Dihydrostreptomycin Gentamicin (as Gentamicin C1, C1a and C2/C2a) Hygromycin B Kanamycin Neomycin B Netilmicin Paromomycin Sisomicin Spectinomycin Streptomycin Tobramycin Ribostamycin (internal standard) Targeted Aminoglycosides: Streptomycin

Transcript of C146-E352 LCMSMS Method Package for Aminoglycoside Antibiotics Method... · Aminoglycosides are...

Page 1: C146-E352 LCMSMS Method Package for Aminoglycoside Antibiotics Method... · Aminoglycosides are very polar compounds, and usually require the use of undesirable ion-pairing reagents

LC/MS/MS Method Package forAminoglycoside Antibiotics

For LabSolutions™ LCMS Software

Complete Solution for Quantitation and Con�rmation of Aminoglycosides in Meat, Eggs and Milk

Aminoglycosides are an antibiotic family widely used for the treatment of bacterial infections in cattle, sheep, pigs and poultry. Regulatory

agencies have set maximum residue limits (MRL) for these compounds after veterinary use. Aminoglycosides are very polar compounds, and

usually require the use of undesirable ion-pairing reagents with LC-MS/MS.

This Method Package is a complete solution for the simultaneous quantitative analysis of all regulated aminoglycosides by HILIC/MS/MS.

It includes:

• A sample preparation protocol using mixed-mode weak cation exchange from Biotage. This protocol produces high recoveries and low matrix

effects, and is adapted to meat, milk or egg samples.

• A primary method for fast (< 5 min) quantitative analysis by HILIC/MS/MS with two MRMs per compound.

• A second method for con�rmation of identity. Using the same mobile phases and column, the gradient is modi�ed and 15 optimized MRMs are

acquired per compound. This enables library searching and accurate identi�cation on Insight Screening using MRM Spectrum Mode (library provided).

LCMS-8060

C146-E352

• Complete method for fast quantitative screening of 13 aminoglycosides in meat, eggs and milk.

• Increase identi�cation con�dence with MRM Spectrum mode and LabSolutions Insight™ Screening.

• No ion-pairing reagent required.• Includes single sample preparation protocol usable for

meat, eggs and milk.

• Amikacin

• Apramycin

• Dihydrostreptomycin

• Gentamicin (as Gentamicin C1, C1a and C2/C2a)

• Hygromycin B

• Kanamycin

• Neomycin B

• Netilmicin

• Paromomycin

• Sisomicin

• Spectinomycin

• Streptomycin

• Tobramycin

• Ribostamycin (internal standard)

Targeted Aminoglycosides:

Streptomycin

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LC/MS/MS Method Package for Aminoglycoside AntibioticsFor LabSolutions LCMS Software

Easy Work�ow

Complete protocol includes step-by-step instructions for sample and calibration curve preparation.

Fast Quantitation and Easy Processing

Perform high-sensitivity analysis with excellent chromatography without

resorting to ion-pairing reagents that foul the mass spectrometer.

LabSolutions Insight provides a fast and intuitive environment for data

processing and review. User-con�gurable �ags alert you what values

are outside of QC limits, facilitating review by exception.

Increased Con�dence in Identi�cation using

MRM Spectrum Mode

MRM Spectrum Mode acquires up to 15 MRMs per analyte, and each

transition is optimized to a speci�c collision energy. Because MS duty

cycle is not wasted on meaningless portions of the spectrum,

information-rich spectra can be acquired in just milliseconds. The

result is an unambiguous spectrum, even for trace-level components.

Note

1. LabSolutions LCMS Ver.5.91 or later is required.

2. LabSolutions Insight Library Screening Ver. 3.1 or later is required.

LabSolutions Insight and LabSolutions are trademarks of Shimadzu Corporation.

Biotage logo is a registered trademark of Biotage AB.

© Shimadzu Corporation, 2018First Edition: April 2018, Printed in Japan 3655-12704-30AIT

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