BioFire (FilmArray) Multiplex PCR Assays

Teresa Finken, MT(ASCP)

Panels Available

Respiratory Viral PanelBlood Culture Identification PanelGastrointestinal Panel

Methodology

Multiplex Real-Time PCR Assay

These panels test for multiple targets simultaneously

This methodology is real-time PCR meaning that it amplifies and detects at the same time

What does this Mean to You?

One sampleOne testMultiple results1.5-hr turnaround time

Respiratory Viral Panel Targets Viruses

Adenovirus

Coronavirus HKU1

Coronavirus NL63

Coronavirus 229E

Coronavirus OC43

Human Metapneumovirus

Human Rhinovirus/Enterovirus

Influenza A

Influenza A/H1

Influenza A/H1-2009

Influenza A/H3tapneumovirus

Influenza B

Parainfluenza 1

Parainfluenza 2

Parainfluenza 3

Parainfluenza 4

Respiratory Syncytial Virus

Bacteria Bordetella pertussis

Chlamydophila pneumoniae

Mycoplasma pneumoniae

Blood Culture Id Panel TargetsGram + Bacteria Enterococcus

Listeria monocytogenes

Staphylococcus

Staphylococcus aureus

Streptococcus

Streptococcus agalactiae

Streptococcus pyogenes

Streptococcus pneumoniae

Yeast Candida albicans

Candida glabrata

Candida krusei

Candida parapsilosis

Candida tropicalis

Antibiotic Resistance mecA - methicillin resistant

vanA/B - vancomycin resistant

KPC - carbapenem resistant

Gram – Bacteria Acinetobacter baumannii

Haemophilus influenzae

Neisseria meningitidis

Pseudomonas aeruginosa

Enterobacteriaceae

Enterobacter cloacae complex

Escherichia coli

Klebsiella oxytoca

Klebsiella pneumoniae

Proteus

Serratia marcescens

Gastrointestinal Panel TargetsBacteria Campylobacter (jejuni, coli and

upsaliensis)

Clostridium difficile (toxin A/B)

Plesiomonas shigelloides

Salmonella

Yersinia enterocolitica

Vibrio (parahaemolyticus, vulnificus and cholerae)

Vibrio cholerae

Diarrheagenic E. coli/Shigella

Enteroaggregative E. coli (EAEC)

Enteropathogenic E. coli (EPEC)

Enterotoxigenic E. coli (ETEC) lt/st

Shiga-like toxin-producing E. coli (STEC) stx1/stx2

E. coli O157

Shigella/Enteroinvasive E. coli (EIEC)

Viruses Adenovirus F 40/41

Astrovirus

Norovirus GI/GII

Rotavirus A

Sapovirus (I, II, IV and V)

Parasites Cryptosporidium

Cyclospora cayetanensis

Entamoeba histolytica

Giardia lamblia

Sample Requirements

Respiratory Viral Panel

o Nasal Wash

o Nasal Swab

o Sputum

o Respiratory specimen in Viral Transport Medium

Gastrointestinal Panel

o Raw stool to be transferred to ETM

o Stool in ETM

Blood Culture ID Panel

o Blood culture bottle that has been flagged positive by the Bacti Alert or Versatrek Analyzers

Positive Results Since Implementaion

These are just a few…

Limited exposure to potentially hazardous bacterial isolates in blood cultures

Faster treatment and more guided treatment of patients at the point of entry and after admission

Quick diagnosis

Faster antimicrobial therapy implementation to speed recovery and reduce morbidity

Admitting a patient versus not admitting a patient

Questions…