1 2 3 4 5 6 7 8 9 100

5

10

15

20

25

30

35

40

number of pc

va

ria

nc

e [

%]

Var

ianc

e (%

)

Number of principle component

Supplemental figure 1

-15 -10 -5 0 5 10 15-15

-10

-5

0

5

10

15

K-218CCP-218K-216

PC-#1

CCP-216

K-214

CCP-214

K-215

CCP-215

PC

-#2

Supplemental figure 2A

-15-10-5051015-10

-8

-6

-4

-2

0

2

4

6

8

10

CCP-215

K-215

CCP-214

K-214

CCP-216

PC-#1

K-216

CCP-218

K-218

PC

-#3

Supplemental figure 2B

-15 -10 -5 0 5 10 15-10

-8

-6

-4

-2

0

2

4

6

8

10

K-215

CCP-215

K-218

K-214

CCP-218

K-216

PC-#2

CCP-216

CCP-214

PC

-#3

Supplemental figure 2C

Supplemental figure 3A

Supplemental figure 3B

Supplemental figure 3C

Supplemental figure 3D

Supplemental figure 3E

1 2 3 4 5 6 7 8 9 100

10

20

30

40

50

60

number of pc

va

ria

nc

e [

%]

Number of principle component

Var

ianc

e (%

)

Supplemental figure 4

-50 -40 -30 -20 -10 0 10 20 30 40 50-30

-20

-10

0

10

20

30

Co.II-214Cd.II-214

Co.II-218

Cd.II-218

Pb.II-214

Pb.II-218

K-218

K-214

PC-#1

Cd.II-215

Co.II-215

Pb.II-215

K-215

PC

-#2

Supplemental figure 5A

-50 -40 -30 -20 -10 0 10 20 30 40 50-25

-20

-15

-10

-5

0

5

10

15

20

25

Co.II-214

Cd.II-214

Co.II-218

Cd.II-218

Pb.II-214

Pb.II-218

K-218

K-214

PC-#1

Cd.II-215Co.II-215Pb.II-215

K-215PC

-#3

Supplemental figure 5B

-50 -40 -30 -20 -10 0 10 20 30 40 50-25

-20

-15

-10

-5

0

5

10

15

20

25

Co.II-214

Cd.II-214

Co.II-218

Cd.II-218

Pb.II-214

Pb.II-218

K-218

K-214

PC-#1

Cd.II-215Co.II-215

Pb.II-215

K-215PC

-#3

Supplemental figure 5B

-30 -20 -10 0 10 20 30-25

-20

-15

-10

-5

0

5

10

15

20

25

K-214

Pb.II-214K-215

Cd.II-214

Co.II-214

PC-#2

K-218Cd.II-218

Pb.II-215

Pb.II-218

Co.II-215

Co.II-218

Cd.II-215

PC

-#3

Supplemental figure 5C

-30 -20 -10 0 10 20 30-25

-20

-15

-10

-5

0

5

10

15

20

25

K-214

Pb.II-214K-215

Cd.II-214

Co.II-214

PC-#2

K-218Cd.II-218

Pb.II-215

Pb.II-218

Co.II-215

Co.II-218

Cd.II-215

PC

-#3

Supplemental figure 5C

Supplemental figures (supplemental online material)

Supplemental figure 1: Variance explained by the individual principle components (corresponding to Figure 1).

Supplemental figure 2: Principle component analysis based on only the first two principle components (corresponding to Figure 1). A. PC-#1: principle component no. 1, PC-#2: principle component no. 2; B. PC-#1: principle component no. 1, PC-#3: principle component no. 3; C. PC-#2: principle component no. 2, PC-#3: principle component no. 3.

Supplemental figure 3: Kegg pathway mapping of genes deregulated by incubation of normal human bronchial epithelial cells with a combination of 15 µg/l Cd(II), 25 µg/l Co(II) and 550 µg/l Pb(II). Dark red colour indicates that the respective gene is at least two-fold upregulated in two patients. Light red colour means upregulation in at least three patients. Similarly, dark green indicates an at least two-fold upregulation in two patients, whereas light green colour highlights genes downregulated in at least three patients. A. Glutathione metabolism, B. Pyrimidine metabolism, C. Purine metabolism, D. Biosynthesis of steroids, E. Cytochrome P450 metabolism.

Supplemental figure 4: Variance explained by the individual principle components (corresponding to Figure 3).

Supplemental figure 5: Principle component analysis based on only the first two principle components (corresponding to Figure 3). A. PC-#1: principle component no. 1, PC-#2: principle component no. 2; B. PC-#1: principle component no. 1, PC-#3: principle component no. 3; C. PC-#2: principle component no. 2, PC-#3: principle component no. 3.