Post on 05-Jan-2016
OXFAM-DELAGUA
Water analysis kit
OXFAM-DELAGUA water analysis kitWATSAN M15 ERUWATSAN M15 ERU 2
Contents
1. Components2. Capabilities3. Maintenance4. Summary of bacteriological analysis process
Título de la presentación
OXFAM-DELAGUA water analysis kitWATSAN M15 ERUWATSAN M15 ERU 3
1. Componentes1. Components
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– Filtration apparatus– Incubator– Culture Medium– Petri Dishes– Sampling Methods– Turbidimeter– pH and Chlorine meters – Consumables
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Filtration apparatus
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Spares case
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External battery
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2. Capabilities
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CAPABILITIES
• Turbidity
• pH
• Free and total chlorine
• Bacteriological analysis (thermotolerant faecal coliforms)
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3. Maintenace
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GENERAL MAINTENANCE
Weekly
• Wash, rinse and dry the filtration apparatus
• Apply a smear of silicone grease to the black rubber O-ring
• Charge the internal battery fully at the end of each week
CAREFULLY READ THE MANUAL
Monthly
• Check the incubator temperature and recalibrate if necessary
• Check all the kit components
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BATTERY
• Never allow the internal battery to discharge fully.
• Never leave the incubator switched on for more than 18 consecutive hours
• Always recharge the battery at the end of the working period in the field.
• Recharge monthly during storage.
CAREFULLY READ THE MANUAL
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4. Summary of the bacteriological analysis process
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• Preparation:– Sterilization of equipment: (autoclave, pressure
cooker, methanol, boiling)– Petri: 15 min in autoclave or with methanol– Filtration apparatus: 15 min with methanol
– Culture medium (38 gr/500 ml distilled water)
– Petri dishes (Up to 16 daily samples)
• Filtration processFiltration volume (10 ml, 50 ml,100 ml) Filtration control
• Incubation (16-18 H 44ºC)
• Counting colonies: Yellow colour (faecal coliforms)
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• Presentation of resultsThe results are presented as CFU/100mlCFU (Colony forming units)
• 100 ml of sample (treated or very clean water) have been filtered: CFU/100ml = nº of identified colonies as coliforms• 50 ml of sample have been filtered: CFU/100ml = nº of identified colonies as coliforms x 2• 10 ml of sample have been filtered: CFU/100ml = nº of identified colonies as coliforms x 10
It is possible to use smaller volumes in samples with higher contamination.
The results should be calculated multiplying by the correspondent dilution factor.
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Thank youThank you……….……….