Color and Absorbance Comparison

Summer 2009

Perkin Elmer Spectrophotometer

• 1 cm path length• 345 & 440 nm wavelenghts• Filtered with 0.45 & 0.22 um cellulose filters• Centrifuged for 30 minutes• Dilutions accounted for in all values

Color Comparison

Color ValuesFiltered 0.45 @ 440

Filtered 0.22 @ 440

Centrifuged @ 345

Filtered 0.45 @ 345

110 %10%

100%

Absorbance Comparison

Absorbance Values

Filtered 0.45 @ 440

Filtered 0.22 @ 440

Centrifuged @ 345

Filtered 0.45 @ 345

510 %

10%

500%