Color and Absorbance Comparison Summer 2009
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Color and Absorbance Comparison Summer 2009
description
Color and Absorbance Comparison Summer 2009. Perkin Elmer Spectrophotometer. 1 cm path length 345 & 440 nm wavelenghts Filtered with 0.45 & 0.22 um cellulose filters Centrifuged for 30 minutes Dilutions accounted for in all values. Color Comparison. Color Values. Filtered 0.45 @ 440. - PowerPoint PPT Presentation
Transcript of Color and Absorbance Comparison Summer 2009
Color and Absorbance Comparison
Summer 2009
Perkin Elmer Spectrophotometer
• 1 cm path length• 345 & 440 nm wavelenghts• Filtered with 0.45 & 0.22 um cellulose filters• Centrifuged for 30 minutes• Dilutions accounted for in all values
Color Comparison
Color ValuesFiltered 0.45 @ 440
Filtered 0.22 @ 440
Centrifuged @ 345
Filtered 0.45 @ 345
110 %10%
100%
Absorbance Comparison
Absorbance Values
Filtered 0.45 @ 440
Filtered 0.22 @ 440
Centrifuged @ 345
Filtered 0.45 @ 345
510 %
10%
500%
