“Integrated CNS and CV monitoring via telemetry in behavior … · 2019-04-11 · PhysioTel...

The Gold Standard in Physiologic Monitoring

“Integrated CNS and CV monitoring via

telemetry in behavior studies”

Thomas Penning

European Manager at Data Sciences International

Measurement Behavior 2012

- Tutorial -

Agenda

DSI Company Overview

New telemetry products for large & small animals

Key features for accurate & reliable chronic monitoring

New possibilities by the use of customized antennas

Examples for combined CNS/CV + behavior monitoring

Discussion, Q&A Session

Questions?

DSI Business Field

DSI was founded in 1984 and

growed to the global leader in

implantable telemetric

physiological monitors

Founded in 1986 as spinout of

Buxco Company focused in

physiological signal analysis

More than 70 years experience in

medical instrumentation and high

reputation for signal conditioning

& recording

Provider of telemetric & hardwired solutions

for cardiovascular, CNS and respiratory

monitoring & analysis in preclinical research

28 Years of history (founded 1984)

180+ Employees

Systems in 48 Countries

18 Sales offices

500,000+ Subjects monitored

1000+ Publications

30+ Patents

20% Revenue spending in R&D

Data Sciences in Figures

Brian Brockway

DSI founder

Implantable Telemetry System

DSI Wireless Monitoring Parameters

Core Body Temperature

Blood Pressure

Respiration (breath frequency)

ECG – electrocardiogram

EMG - electromyogram

EOG – electro-oculogram

EEG – electroencephalogram

LVP, PAP,

Intra-Pleural Pressure

Bladder Pressure

Intracavernosal Pressure

Many More…

Implantable Family

Wide application

Reduced stress

Minimized variables

Increased data accuracy

Pressure Temperature

Biopotential

New Telemetry Offerings

Physiotel HD Series

Physiotel Digital

Receiver RSC-1 / RSC-3

DSI Business Services

What is ‘HD’?

HD = Hybrid Digital telemetry…

New transmitter for small animals

Improved reliability & performance

More signals & parameter combinations

Provide improved reliability & performance.

Improved animal welfare

Works with existing hardware*

* Software update required

PhysioTel HD Platform Overview

Digital encoded data

Device ID & serial number

Calibration values

Battery status and battery “ON” time

High performance pressure sensing

No wrong data due crosstalk

Automated Xmitter configuration

HD-S21 Dual-pressure Small Animal Telemetry

Signals Measured

Two pressures

Biopotential

Temperature

Activity

Intended Species

Ferret

Guinea pig

Marmoset

Battery: 2 month

Volume: 5.9cc

Weight: 8g

Implantable at an animal body weight of 175g

Application opportunities with HD-S21

Chronic monitoring applications:

– Left ventricular pressure + blood pressure (BP)

– Pulmonary artery pressure + BP

– Pleural pressure + BP

– Pulse wave velocity

– Intra-ocular pressures

– Uterus pressure + BP

– Bladder pressure + BP

Precise, reliable, high fidelity data is

available with an HD-S21.

LVP, BP & ECG with HD-S21

HD-S21 Chronic Parameter Data

HD-X11 Transmitter

Signals Measured

Pressure

ECG, EMG or EEG

Temperature

Activity

Intended Species

Hamster

Juvenile rat

Plus more!

Volume: 1.4cc

Weight: 2.2g

Battery Life: 30 days

IP implantable from a min. body weight of 30g

SC implantable from a min. body weight of 19g

New Capabilities with the HD-X11

Consolidated study of multiple

parameters:

Reduce in animal use

Higher statistical relevance

More complete physiologic

assessment in CV studies

HD-X11 components

held together with

flexible circuit

Simultaneous BP & ECG from a Single Mouse!

HD-X11 parameter data

PhysioTel Digital Implants

Model L21 2 Pressures

1 Biopotential

Temperature

Activity

Battery Life: 12 weeks

Model L11 1 Pressure

1 Biopotential

Temperature

Activity

Battery Life: 15 weeks

Technical

Spec Comparison

D70 - PCT L11

Weight 47grams 56 grams

Volume 32cc 29cc

Pressure Drift < 2 mmHg/month max. < 2 mmHg/month max.

Battery Life 14 weeks 15 weeks

RF - Range 1m 3-5m

Bandwidth Biopotential 1 – 100Hz 0.1 – 100Hz

Biopotential Range Fixed Programmable

Implantation in Primates

BP catheter with

radio opaque marker

Intravascular (negative) ECG

Leadin superior vena cava

Positive ECG lead

Antenna inserted in an

intramuscular pocket

System Equipment

In the animal

In the cage

In the room

DSI Telemetry

PhysioTel

L21, L11

PhysioTel

Digital

Key specs of the system

Digital data

No cross talk

Less disturbances

Auto-configuration

Longer range (3-5m)

Less receivers

Multi animals/receiver

Group housing (up to 12)

Example Canine System

Accurate Biopotential Sensing

Biopotential

Expanded bandwidth 0.1 – 100 Hz

Solid Tip electrode for clean ECGs

Primate Data

Dog Data

Minipig Data

Example Primate Set-up

Surgery Support for DSI Products

Training Workshops

Quarterly courses at DSI

Harlan Courses in EU

Labs at AALAS,CALAS, ASR

Surgical Support Services:

Surgical Videos and manuals

Experienced surgeons at DSI

Customer reference sites

Trained implant services vendors

Taconic

Charles River Laboratories

The Jackson Laboratory

DSI Support

Trained Technical Sales Representatives,

Tech Support Staff

Keys for accurate chronic monitoring

Pressure Sensing Techniques

Battery challenges

Sampling frequency and bandwidth

Housing and environment

Sensing Technology Considerations

Summary – Sensing Technology

Good adoption of frequency to phys. signals

High temperature stability (less drift)

Proven reliability in chronic applications

Easy to remove without damage

Inexpensive replacement (refurbishment)

Antithrombotic & proven biocompatibility

Maintenance cost

Self maintenance with re-gel procedure

Transmitter exchange program

Durablity Cost

solid tip fluid filled

500 €

1,000 €

1,500 €

2,000 €

2,500 €

3,000 €

HD-X11 new Refurbishment

Exchange Program

Factory site renewal of:

Catheter

Lead electrodes

Housing

Battery

Calibration

Sterilization

Sterile Package

Last approx. 3 weeks

Returned transmitter “As New”

Warranty of up to 18 month*

* MWP = Maximum Warranty Period (Type depended)

Signal Frequency Characteristic

Drop in amplitude

Slow rise time

Inaccurate contractility

Increase of noise

More disturbances

Same physiol. info

higher

Bandwidth

smaller

Bandwidth Hz kHz

The frequency characteristic of a catheter is the ability to follow

pressure changes over time. The higher the HR the higher needs to

be the bandwidth of the catheter.

LVP signal in the frequency domain

HR = 320bpm = 5.3 Hz This amplitude spectrum shows which

catheter frequency would be necessary

for a accurate reproduction of a

ventricular pressure signal.

Higher frequency portions then the heart

frequency are seen due the non-

sinusoidal shape of the LVP signal

Catheter Frequency Characteristics

The frequency characteristic of

a catheter depends on material

and length.

The shorter the catheter the

higher the bandwidth

Empirically the bandwidth for

BP monitoring should be

5 times higher than the heart

frequency.

Rat LVP – two catheter types Time rel. Time abs. Date Num Sys LVEDP DP HR dP/dt Time rel. Time abs. Date Num Sys LVEDP DP HR dP/dt

0000:29:53 11:18:15 1/27/2009 397 151 21 131 320 9394 0000:29:53 11:18:15 1/27/2009 397 151 21 131 320 9111

0000:29:53 11:18:15 1/27/2009 398 151 20 131 320 9607 0000:29:53 11:18:15 1/27/2009 398 151 20 131 320 9138

0000:29:53 11:18:15 1/27/2009 399 150 19 131 321 9603 0000:29:53 11:18:15 1/27/2009 399 150 20 130 320 9192

0000:29:54 11:18:16 1/27/2009 400 149 19 129 318 9394 0000:29:54 11:18:16 1/27/2009 400 149 19 129 319 9151

Catheter Bandwidth for BP Monitoring

higher

Bandwidth

smaller

Bandwidth

Drop in amplitude

Slow rise time

Inaccurate contractility

Increase of noise

More disturbances

Same physiol. info

Hz kHz

Depends on the HR of the animal

BP Rat: 300bpm (5Hz) * 5 = 25Hz

Mouse 900bpm (15Hz) * 5 = 75Hz

Drift Compensation

Bridge in DSI transmitter housing Bridge in solid tipped catheters

Cath. Tip

Electronic housing

Sensing technology of HD

devices have a small

temperature drift and long

term offset drift due

advanced compensation

technology

Reliability / Patency

DSI Pressure Sensing Catheter

Solid Tip Catheter

Non-Compressible Gel

Fragile latex membrane

Bloodstream

around pressure

sensing tip and

antithrombotic

treatment

prevents clothing

Long term

reliability of the

gel-tip tested and

proved in 1000+

chronic studies.

Charging Challenges

No defined distance between charger & implant.

Energy transmission creates heat

Impact on temperature monitoring

Impact on animal behavior

Impact on animal welfare

Electrical field can create disturbances

More artifacts in signal recording

Makes refurbishment not obsolete

Necessary exchanges of catheter & lead material

Recommended annual factory re-calibration

Battery Operation

Lifetime specified for continuous operation

Battery can be turn ON/OFF by a magnet,

Exchange by refurbishment procedure,

Advantages of Battery Devices:

Longer operation times without maintenance,

Known battery status,

Vendor warranted operation time,

No recharging required (impact on behavior and temperature)

No tissue irritations

Accurate temperature monitoring

Typical Battery Lifetime:

PA-C10 = 1.5month; CTA-F40 = 6 month; TA-F40 = 11month

What’s the max. sampling rate?

Limited by Carrier Frequency

Typically 5x transmitter bandwidth

Sampling rate can be increased by interpolation

Example:

TF50-W-F2 for Sympathetic Nerve Analysis (SNA)

Bandwidth: 1kHz

Sampling rate: 5kHz

Use of customized antennas

Transmission range restricted by energy consumption

Other influences on transmission ranges: Interferences

Metal shielding

Environmental noise

Small animal implant HD-X11

Large animal implant D70-EEE

Large animal implant PT Digital

Small animal implant HD-X11 Large animal implant D70-EEE Large animal implant PT Digital

Series1 0.2 1 5

Transmission range / m

Behavioral data: running wheels, drinking and feeding activity, stimulus, etc.

Combine with video based Behavior Analysis. DSI data into Observer XT/Noldus

Combine with other Behavioral analysis systems: Laboras/Metris

Direct and Dynamic data exchange with Excel: scoring, develop homemade systems, Control output

Telemetry + Environmental + Behavioral data

Telemetry + Environmental data

Telemetry + Behavioral data

Food intake behavior and CV monitoring

CNS and Behavior Monitoring

FAQ’s

How can I interface between Noldus behavior systems

and DSI telemetry?

“Integrated CNS and CV monitoring via telemetry in behavior … · 2019-04-11 · PhysioTel...

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Transcript of “Integrated CNS and CV monitoring via telemetry in behavior … · 2019-04-11 · PhysioTel...

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