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The Basic Local Alignment Search Tool (BLAST) Rapid data base search tool (1990) Idea: (1) Search for high scoring segment pairs The Basic Local Alignment Search Tool (BLAST)…
What is BLAST? BLAST® (Basic Local Alignment Search Tool) is a set of similarity search programs designed to explore all of the available sequence databases regardless…
CAS REGISTRYSM: BLAST® similarity searching via STN Express® November 2008 Table of Contents Overview of content 3 Accessing CAS REGISTRY BLAST 4 Searching similar sequences…
Slide 1Alignment methods Introduction to global and local sequence alignment methods Global : Needleman-Wunch Local : Smith-Waterman Database Search BLAST FASTA Slide 2 Why…
BLAST: Basic Local Alignment Search Tool Altschul et al J Mol Bio 1990 CS 466 Saurabh Sinha Motivation • Sequence homology to a known protein suggest function of newly…
Slide 1 BLAST and Multiple Sequence Alignment Learning objectives-Learn the basics of BLAST, Psi-BLAST, and multiple sequence alignment Slide 2 Which program should one use?…
BLASTBLAST: Excerpts by Winfried Just www.bioalgorithms.info Outline www.bioalgorithms.info Encountering a new word: “rucksack” Meaningless without a dictionary
Point Specific Alignment Methods PSI â BLAST & PHI â BLAST In order to control the quality of the sequence matches in a BLAST search controls are placed on the E â…
Slide 1 Point Specific Alignment Methods PSI – BLAST & PHI – BLAST Slide 2 In order to control the quality of the sequence matches in a BLAST search controls are…
Point Specific Alignment Methods PSI â BLAST & PHI â BLAST Even using Local Sequence Alignment Techniques and Scoring Matrices such as high powers of PAM or low values…
Local Alignment and BLAST Three key questions Query? Purpose? Database? BLAST – the way it used to look >gi|77630012|ref|ZP_00792598.1| COG0442: Prolyl-tRNA synthetase…
OCICB Presentation Template 1 Lecture 2 BLAST and Sequence Alignment Phylogenetics and Sequence Analysis Fall 2015 Kurt Wollenberg, PhD Phylogenetics Specialist Bioinformatics…
Database Searching BLAST Database Searching Sequence Alignment Scoring Matrices Significance of an alignment BLAST, algorithm BLAST, parameters BLAST, output Alignment significance…
Jonathan M. Urbach Uses of BLAST Types of BLAST How BLAST works Scanning for 'hits' Interpretation of Blast Results Learning More About BLAST, gi|13325078|gb|AAG33875.2|
Joyce Njoki Nzioki BecA-‐ILRI Hub, Nairobi, Kenya h;p://hub.africabiosciences.org/ h;p://www.Ilri.org/ [email protected] Basic Local Alignment…
An Essential Guide to the Basic Local Alignment Search Tool Ian Korf, Mark Yandell Joseph Bedell BLAST Foreword by Stephen Altschul BLAST Other resources from O’Reilly…
Wilson Leung 12242019 1 An Introduction to NCBI BLAST Prerequisites: Detecting and Interpreting Genetic Homology: Lecture Notes on Alignment Resources: The BLAST web server…
1. Sucheta Tripathy, 16th November 2012 2. A protein sequence from species A◦ What is the nearest species this protein is similarto?◦ Where is it originated from?◦…
How to Run NCBI BLAST on zcluster at GACRC BLAST: Basic Local Alignment Search Tool Georgia Advanced Computing Resource Center University of Georgia Suchitra Pakala pakala@ugaedu…
Local alignment, BLAST and Psi-BLAST October 25, 2012 Local alignment Quiz 2 Learning objectives-Learn the basics of BLAST and Psi-BLAST Workshop-Use BLAST2 to determine…