The application of DNA technology in Animal Forensic and Parentage Determination
Presented by: Ms. N. Mapholi (MSc in Genetics)
ARC-API: Animal Genetics Lab• Animal forensics in livestock and game poaching• Cattle and horse DNA profiling and Parentage
Determination• Species Identification on meat and process meat• Genetic Characterization• Genetic Defects tests• LIDCAT system• Gene mapping and linkage studies
Technologies
1. Genotyping– Microsatellites marker panels– Species specifics panels
2. Real time PCR – Primer designed kits– SNPs assays
FORENSIC WORK
Stock theft and poaching• Microsatellites markers
– Link the crime scene and the suspect DNA evidences obtained
– Parentage dispute cases– Different marker panels
• Bovine, Horse, Ovine, Goat, Porcine, Buffalo, Impala, Kudu, Wildebeest, Eland, Sable, Rhino and Blesbok
3130xL
Chain of custody
Herd of sheep Crime scene New case
DNA SamplesThermal cyclerGene scan DNA extract
Results Interpretation
JudgementJail
CourtDNA Report
Parentage dispute• DNA samples from both parents and calf• Alleged mothers and dispute calf • It is important to take note that in the
absence of either parent (bull or cow) paternity cannot be proven.
• Only when both parents are analyzed it can be proven beyond doubt that the parents are the real biological parents of the offspring in question
Parentage disputeCow 1 Cow 2
Calf 1?
Inclusion/ exclusion as possible mothers
…
Hair Samples PCR tubes PCR tray
Genetic analyzer PCR machine
Procedure of sampling hair
DNA profiling
• Service rendered to the breed societies and stud breeders for bovine and horses
• DNA profiling certificates are issued• Parentage determination for breeding and
selection purpose• Buffalo DNA profiling – this is new and still
building the database
Real Time PCR• DNA based technology• Species identification on meat and processed
meat products – biltong, wors, patties, sausages, mince meat,
Vienna• Species specific primer designed kits• SNP Assays for genetic defects tests in
bovine and porcine – Dexter chondodysplasia and Malignant
hyperthermia
Genetic characterization
• Most of livestock species haven been characterized in our lab using microsatellites markers
• Several multiplexes have been developed and optimized in our lab
LIDCAT DATABASE
• Lidcat package – for tail hair collection
Information needed on LIDCAT system
• Animal ID (ear tag number)• Species (cattle)• Breed (Nguni)• Gender (female or male)• Name of the owner (Olivia Mapholi)• Postal address of owner• Contact numbers
Contact detailsMs. Olivia Mapholi
Tel : 012 672 9027/ 9008/ 9187Fax: 012 672 9214
Thank you
Any questions?
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