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Supplementary Figure 1

IRIRACCTCCCTCTGACGAGGGAGGTGGCTCGACGGAGTCGAGACGGAGGGGATAGAATCCTACGCTGGCCGCCAAAAAGCGCGTGAAAAGCC

GTCTTCAGTCGCTTCAAGGCGGTAGTCTGAACAATGTTCGAGTAATTTAGAAGGAGAATCAAGGGTG*

-10 RBS

clpP1

-35

T G C A 43°C 47 °C

T

suis : CCGCCTCCTCATTGACGACGTC-CTCGACACCTTGGAAT---AACCCGAAAT-CCCTACGCTACCCGCCAAAA-GCACGCGAAAAGCCGCCTTCAGTCGCTglobosum_ : CCGCCTCCTCATTGACGACGTGGCTGGACACCTTGGAAT---AACCCGAAAT-CCCTACGCTACCCGCCAAAA-GCACGCGAAAAGCCGCCTTCAGTCGCTinfantis_ : CCGCCTCCTCATTGACGACGTC-CTCGACACCCTCGAAT---AAACCGAAAT-CCCTACGCTGCCCGCCAAAAAGCACGCGAAAAGCCGCCTTCAGTCGCTlongum : CCGCCTCCTCATTGACGACGTC-CTCGATACCTTGGAAT---AA-CCGAAAT-CCCTACGCTGCCCGCCAAAAAGCACGCGAAAAGCCGCCTTCAGTCGCTbreve : CCTCCCTCTGACGAGGGAGGTGGCTCGACGGAGTCGAGACGGAGGGAGAGATATCCTACGCTGGCCGCCAAAAAGCGCGTGAAAAGCCGTCTTCAGTCGCTdentium : TCAACAAAAGACGAGGGAGGTGGCTCGACAAAGTCGAGACGGAGGGAGAGATATCCTACGCTACCCGCCAAAAAGCACGCGAAAAGCCGACTTCAGTCGCT suis : TCAAGGCGGTAGTCTGAACAATGTTCGAGTAATT-AGAAGGAGAATCAAGGTGglobosum_ : TCAAGGCGGTAGTCTGAACAATGTTCGAGTAATT-AGAAGGAGAATCAAGGTGinfantis_ : TCAAGGCGGTAGTCTGAACAATGTTCGAGTAATT-AGAAGGAGAATCAAGGTGlongum : TCAAGGCGGTAGTCTGAACAATGTTCGAGTAATT-AGAAGGAGAATCAAGGTGbreve : TCAAGGCGGTAGTCTGAACAATGTTCGAGTAATTTAGAAGGAGAATCAAGGTGdentium : TCAAGGCGGTAGTCTGAACAATGTTCGAGTAATT-AGAAGGAGAATCAAGGTG

RBS

B. suis LMG 21814B. globosum JCM 5820B. infantis JCM 1222B. longum NCC2705B. breve UCC 2003B. dentium JCM 1195

-10

B. suis LMG 21814B. globosum JCM 5820B. infantis JCM 1222B. longum NCC2705B. breve UCC 2003B. dentium JCM 1195

*

IR IR -35

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