Supplementary Figure 1
description
Transcript of Supplementary Figure 1
Supplementary Figure 1
PIG3
cata
lase
MW(kDa)
72
55
43
34
p53
cata
lase
MW(kDa)
7255
43
34
26
95130170
AA BB
-tubulin
catalase
PIG3
VectorPIG3
Cat
alas
e ac
tivi
ty (
U/m
l)
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
H1299 HCT116 p53-/-SW480
AA
DC
F f
luor
esce
nce
leve
l
VectorPIG3
H1299
HCT116 p53-/-
SW480
0
20
40
60
80
100
120
140
160
BB
Supplementary Figure 2
HCT116
siRNA
U2OS RKO
Control p53
p53
p53R2
SESN1
SESN2
GPX1
ALDH4A1
GAPDH
catalase
SOD2
TIGAR
Control p53 Control p53
TP53INP1
Nrf-2
Supplementary Figure 3
catalase
p53
0 4 8 12 24 0 4 8 12 24 (hr)
HCT116 p53-/-
UV (10 J/m2)
-tubulin
HCT116
p53R2
0 0.05 0.1 1 2 0 0.05 0.1 1 2
HCT116 p53-/-
H2O2 (mM)
HCT116
UV (10 J/m2) H2O2 (mM)
0 0.05 0.1 1 2 0 0.05 0.1 1 2
Saos-2 U2OS
catalase
p53
0 4 8 12 24 0 4 8 12 24 (hr)
Saos-2
-tubulin
U2OS
p53R2
Supplementary Figure 4
MW(kDa)
72
55
43ca
tala
se
p53
R2
Supplementary Figure 5
Cat
alas
e ac
tivi
ty (
%)
0
50
100
150
200
250
vector wt R2 R4
n.s.
AA
RO
S le
vels
(%
)
0
20
40
60
80
100
120
vector wt R2 R4
n.s.
Supplementary Figure 6
BB
AABB
HCT116U2OS RKO
p53R2
catalase
-tubulin
Cat
alas
e ac
tivi
ty (
U/m
l)
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
n.s.
Control siRNAp53R2 siRNA
HCT116 U2OS RKO0
20
40
60
80
100
120
140
160
n.s.
Control siRNAp53R2 siRNA
Supplementary Figure 7
RO
S le
vels
(%
)
p53R2
catalase
-tubulin
Cat
alas
e ac
tivi
ty (
U/m
l)
HCT116U2OS RKO
AA BB
0.0
0.5
1.0
1.5
2.0
2.5
0
20
40
60
80
100
120
HCT116U2OS RKO
n.s.
n.s.
n.s.
n.s.
n.s.
n.s.
Supplementary Figure 8
Control vectorWild-type p53R2 Mutant p53R2 (V115L)
RO
S le
vels
(%
)
Control vectorWild-type p53R2 Mutant p53R2 (V115L)
p53R2
PIG3
Catalase
p53
β-actin
Sup P
UT
Sup P
PMP70
UV (10 J/m2)
Supplementary Figure 9
UT
catalase merge DAPI
p53
p53R2
p53
p53R2
Supplementary Figure 10
UV (10 J/m2)
0
20
40
60
80
100
120
140
160
180
200
0 8 24 h
Supplementary Figure 11
AA BB
0
5
10
15
20
25
30
35
0 24 48 h
Ap
opto
sis
(%)
UV (40 J/m2)
U2OS (vector)U2OS (catalase)
UV (40 J/m2)
RO
S le
vels
(%
)
U2OS (vector)U2OS (catalase)
0 48 hAd p53
0
20
40
60
80
100
120
140
160
180
H1299 (vector)H1299 (catalase)
Supplementary Figure 12
RO
S le
vels
(%
)
Untreated controlUV (20 J/m2)
HCT116 p53-/-
Vector PIG3
0
20
40
60
80
100
120
140
160
180
200
Supplementary Figure 13
RO
S le
vels
(%
)