Smoking behavior associated genesNicotinic Acetylcholine Receptor Families

(CHRNA 1-10 & CHRNB 1-4), CYP2A6

Search for SNPs in 3’UTR that lie in the seed region of a miRNAa)PolymiRTS (320 SNPs)

b)SNPInfo (109 SNPs)c)Patrocles (15 SNPs)

Included if predicted by at least 2 programs

MAF > 5%

Allele causes predicted change in thermodynamic interaction with 3’UTR

(RNA Hybrid)

Successful iPlex design for genotyping assay

Genotyped in NCI-MD Case Control Study Test cohort of 316 controls and 319 cases

(European American)

rs686 in DRD1 (modulator of miRNA binding

SNP from literature)

12 SNPs

4 SNPs

4 SNPs

3 SNPs

3 SNPs

Supplementary Figure 1: Flowchart detailing the selection process of SNPs. Rs686 in DRD1 was selected from the literature as being a miRNA-binding modulating SNP (Huang and Li, 2009 Biol Pschiatry, 65 9 702-705)