Smoking behavior associated genes
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Smoking behavior associated genes Nicotinic Acetylcholine Receptor Families (CHRNA 1-10 & CHRNB 1-4), CYP2A6 Search for SNPs in 3’UTR that lie in the seed region of a miRNA a)PolymiRTS (320 SNPs) b)SNPInfo (109 SNPs) c)Patrocles (15 SNPs) Included if predicted by at least 2 programs MAF > 5% Allele causes predicted change in thermodynamic interaction with 3’UTR (RNA Hybrid) Successful iPlex design for genotyping assay Genotyped in NCI-MD Case Control Study Test cohort of 316 controls and 319 cases (European American) rs686 in DRD1 (modulator of miRNA binding SNP from literature) 12 SNPs 4 SNPs 4 SNPs 3 SNPs 3 SNPs Supplementary Figure 1: Flowchart detailing the selection process of SNPs. Rs686 in DRD1 was selected from the literature as being a miRNA-binding modulating SNP (Huang and Li, 2009 Biol Pschiatry, 65 9 702-705)
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Smoking behavior associated genes Nicotinic Acetylcholine Receptor Families (CHRNA 1-10 & CHRNB 1-4), CYP2A6. rs686 in DRD1 (modulator of miRNA binding SNP from literature). Search for SNPs in 3’UTR that lie in the seed region of a miRNA PolymiRTS (320 SNPs) SNPInfo (109 SNPs) - PowerPoint PPT Presentation
Transcript of Smoking behavior associated genes
Smoking behavior associated genesNicotinic Acetylcholine Receptor Families
(CHRNA 1-10 & CHRNB 1-4), CYP2A6
Search for SNPs in 3’UTR that lie in the seed region of a miRNAa)PolymiRTS (320 SNPs)
b)SNPInfo (109 SNPs)c)Patrocles (15 SNPs)
Included if predicted by at least 2 programs
MAF > 5%
Allele causes predicted change in thermodynamic interaction with 3’UTR
(RNA Hybrid)
Successful iPlex design for genotyping assay
Genotyped in NCI-MD Case Control Study Test cohort of 316 controls and 319 cases
(European American)
rs686 in DRD1 (modulator of miRNA binding
SNP from literature)
12 SNPs
4 SNPs
4 SNPs
3 SNPs
3 SNPs
Supplementary Figure 1: Flowchart detailing the selection process of SNPs. Rs686 in DRD1 was selected from the literature as being a miRNA-binding modulating SNP (Huang and Li, 2009 Biol Pschiatry, 65 9 702-705)
