Cédric Berney - Recent Contributions to the Eukaryote Tree of Lifepresent address: Protist Evolution & Pelagic Ecosystems (Dr Colomban de Vargas), UMR 7144, CNRS/UPMC, Station Biologique, Roscoff, Franceformer affiliation: Genomics and Microbial Diversity (Dr David Bass), Life Sciences, The Natural History Museum, London, UK

Large, branching and/or network-forming heterotrophic naked amoebae arecommon in soils and in freshwater & marine sediments, and are suspected to playimportant roles in microbial ecosystems. Such amoebae evolved convergently inmany eukaryotic groups (mostly Amoebozoa and Rhizaria) and are often closelyrelated to major parasite groups of economic importance. Yet they remain verypoorly understood. For the majority of described species, molecular data are stillmissing, and few lineages have been studied morphologically or ecologically.

As part of NERC-funded projects, David Bass and I explored the molecular,ecological, and morphological diversity of these amoebae, including Vampyrellida(top left box) and Variosea (top right box). Using lineage-specific 18S primers, weexplored hidden diversity within Ascetosporean parasites (bottom left box). Wealso investigated rhizarian lineages known only from environmental data andcould characterise some of them morphologically. We used a combined 18S-28Sdataset to resolve deep relationships within Rhizaria (bottom right box).

Vampires in the oceans: predatory cercozoan amoebae in marine habitats Expansion of the 'reticulosphere':diversity of novel branching and network-forming amoebae helps to defineVariosea (Amoebozoa)

A combined 18S-28S rDNA dataset to elucidate higher-levelrelationships within Rhizaria, with focus on Endomyxa

Cédric Berney, Sarah Romac, Frédéric Mahé, Sébastien Santini, Raffaele Siano, & David Bass. 2013. ISME J 7: 2387-2399

Cédric Berney, Stefan Geisen, Jeroen Van Wichelen, Frank Nitsche,Pieter Vanormelingen, Michael Bonkowski, & David Bass.2015. Protist 166: 271-295

Cédric Berney, Rachel Foster, Georgia Ward, Akinori Yabuki, Takashi Shiratori, Roberto Sierra, Jan Pawlowski,& David Bass. in preparation

Mikrocytids are a broadly distributed and divergent radiation ofparasites in aquatic invertebrates

Hanna Hartikainen, Oliver Ashford,Cédric Berney, Beth Okamura,Stephen Feist, Craig Baker-Austin,Grant Stentiford, & David Bass.2014. ISME J 8: 177-186

Lineage-specific molecularprobing reveals novel diversityand ecological partitioningof Haplosporidians

Hanna Hartikainen, Grant Stentiford,Kelly Bateman, Cédric Berney,Stephen Feist, Matt Longshaw,Beth Okamura, David Stone,Georgia Ward, Charlotte Wood,& David Bass. 2014.Current Biology 24: 807-812

Arachnula sp. from Wales

Thalassomyxa sp. from Majorca

Darbyshirella terrestris,n. gen., n. sp

Dictyamoeba vorax, n. gen., n. sp.

Arboramoeba reticulata, n. gen., n. sp.

RETARIAForaminifera+ Radiolaria

Penardia sp. from North Carolina

"ZOORHIZA"Filoreta + Gromia + Ascetosporea + env. lineagesEndo4, Endo5, Endo6 (identified as a flagellate)

"EORHIZA"Tremula + env. novel clade 12 + env. novel clade 10(identified as exclusively FW/soil flagellates)

"PHYTORHIZA"Vampyrellids + Phytomyxea + env. novel clade 9(identified as parasites of diatoms and brown algae)

emended FILOSA / CERCOZOAchlorarachniophytes, cercomonads, euglyphids, etc.

environmental novel clade 9parasites of diatoms and brown algae

environmental lineage Endo6(E

NDOM

YXA)