Recent Contributions to the Eukaryote Tree of Life
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Cédric Berney - Recent Contributions to the Eukaryote Tree of Life present address: Protist Evolution & Pelagic Ecosystems (Dr Colomban de Vargas), UMR 7144, CNRS/UPMC, Station Biologique, Roscoff, France former affiliation: Genomics and Microbial Diversity (Dr David Bass), Life Sciences, The Natural History Museum, London, UK Large, branching and/or network-forming heterotrophic naked amoebae are common in soils and in freshwater & marine sediments, and are suspected to play important roles in microbial ecosystems. Such amoebae evolved convergently in many eukaryotic groups (mostly Amoebozoa and Rhizaria) and are often closely related to major parasite groups of economic importance. Yet they remain very poorly understood. For the majority of described species, molecular data are still missing, and few lineages have been studied morphologically or ecologically. As part of NERC-funded projects, David Bass and I explored the molecular, ecological, and morphological diversity of these amoebae, including Vampyrellida (top left box) and Variosea (top right box). Using lineage-specific 18S primers, we explored hidden diversity within Ascetosporean parasites (bottom left box). We also investigated rhizarian lineages known only from environmental data and could characterise some of them morphologically. We used a combined 18S-28S dataset to resolve deep relationships within Rhizaria (bottom right box). Vampires in the oceans: predatory cercozoan amoebae in marine habitats Expansion of the 'reticulosphere': diversity of novel branching and network- forming amoebae helps to define Variosea (Amoebozoa) A combined 18S-28S rDNA dataset to elucidate higher-level relationships within Rhizaria, with focus on Endomyxa Cédric Berney, Sarah Romac, Frédéric Mahé, Sébastien Santini, Raffaele Siano, & David Bass. 2013. ISME J 7: 2387-2399 Cédric Berney, Stefan Geisen, Jeroen Van Wichelen, Frank Nitsche, Pieter Vanormelingen, Michael Bonkowski, & David Bass. 2015. Protist 166: 271-295 Cédric Berney, Rachel Foster, Georgia Ward, Akinori Yabuki, Takashi Shiratori, Roberto Sierra, Jan Pawlowski, & David Bass. in preparation Mikrocytids are a broadly distributed and divergent radiation of parasites in aquatic invertebrates Hanna Hartikainen, Oliver Ashford, Cédric Berney, Beth Okamura, Stephen Feist, Craig Baker-Austin, Grant Stentiford, & David Bass. 2014. ISME J 8: 177-186 Lineage-specific molecular probing reveals novel diversity and ecological partitioning of Haplosporidians Hanna Hartikainen, Grant Stentiford, Kelly Bateman, Cédric Berney, Stephen Feist, Matt Longshaw, Beth Okamura, David Stone, Georgia Ward, Charlotte Wood, & David Bass. 2014. Current Biology 24: 807-812 Arachnula sp. from Wales Thalassomyxa sp. from Majorca Darbyshirella terrestris, n. gen., n. sp Dictyamoeba vorax, n. gen., n. sp. Arboramoeba reticulata, n. gen., n. sp. RETARIA Foraminifera + Radiolaria Penardia sp. from North Carolina "ZOORHIZA" Filoreta + Gromia + Ascetosporea + env. lineages Endo4, Endo5, Endo6 (identified as a flagellate) "EORHIZA" Tremula + env. novel clade 12 + env. novel clade 10 (identified as exclusively FW/soil flagellates) "PHYTORHIZA" Vampyrellids + Phytomyxea + env. novel clade 9 (identified as parasites of diatoms and brown algae) emended FILOSA / CERCOZOA chlorarachniophytes, cercomonads, euglyphids, etc. environmental novel clade 9 parasites of diatoms and brown algae environmental lineage Endo6 (ENDOMYXA)
Transcript of Recent Contributions to the Eukaryote Tree of Life
Cédric Berney - Recent Contributions to the Eukaryote Tree of Lifepresent address: Protist Evolution & Pelagic Ecosystems (Dr Colomban de Vargas), UMR 7144, CNRS/UPMC, Station Biologique, Roscoff, Franceformer affiliation: Genomics and Microbial Diversity (Dr David Bass), Life Sciences, The Natural History Museum, London, UK
Large, branching and/or network-forming heterotrophic naked amoebae arecommon in soils and in freshwater & marine sediments, and are suspected to playimportant roles in microbial ecosystems. Such amoebae evolved convergently inmany eukaryotic groups (mostly Amoebozoa and Rhizaria) and are often closelyrelated to major parasite groups of economic importance. Yet they remain verypoorly understood. For the majority of described species, molecular data are stillmissing, and few lineages have been studied morphologically or ecologically.
As part of NERC-funded projects, David Bass and I explored the molecular,ecological, and morphological diversity of these amoebae, including Vampyrellida(top left box) and Variosea (top right box). Using lineage-specific 18S primers, weexplored hidden diversity within Ascetosporean parasites (bottom left box). Wealso investigated rhizarian lineages known only from environmental data andcould characterise some of them morphologically. We used a combined 18S-28Sdataset to resolve deep relationships within Rhizaria (bottom right box).
Vampires in the oceans: predatory cercozoan amoebae in marine habitats Expansion of the 'reticulosphere':diversity of novel branching and network-forming amoebae helps to defineVariosea (Amoebozoa)
A combined 18S-28S rDNA dataset to elucidate higher-levelrelationships within Rhizaria, with focus on Endomyxa
Cédric Berney, Sarah Romac, Frédéric Mahé, Sébastien Santini, Raffaele Siano, & David Bass. 2013. ISME J 7: 2387-2399
Cédric Berney, Stefan Geisen, Jeroen Van Wichelen, Frank Nitsche,Pieter Vanormelingen, Michael Bonkowski, & David Bass.2015. Protist 166: 271-295
Cédric Berney, Rachel Foster, Georgia Ward, Akinori Yabuki, Takashi Shiratori, Roberto Sierra, Jan Pawlowski,& David Bass. in preparation
Mikrocytids are a broadly distributed and divergent radiation ofparasites in aquatic invertebrates
Hanna Hartikainen, Oliver Ashford,Cédric Berney, Beth Okamura,Stephen Feist, Craig Baker-Austin,Grant Stentiford, & David Bass.2014. ISME J 8: 177-186
Lineage-specific molecularprobing reveals novel diversityand ecological partitioningof Haplosporidians
Hanna Hartikainen, Grant Stentiford,Kelly Bateman, Cédric Berney,Stephen Feist, Matt Longshaw,Beth Okamura, David Stone,Georgia Ward, Charlotte Wood,& David Bass. 2014.Current Biology 24: 807-812
Arachnula sp. from Wales
Thalassomyxa sp. from Majorca
Darbyshirella terrestris,n. gen., n. sp
Dictyamoeba vorax, n. gen., n. sp.
Arboramoeba reticulata, n. gen., n. sp.
RETARIAForaminifera+ Radiolaria
Penardia sp. from North Carolina
"ZOORHIZA"Filoreta + Gromia + Ascetosporea + env. lineagesEndo4, Endo5, Endo6 (identified as a flagellate)
"EORHIZA"Tremula + env. novel clade 12 + env. novel clade 10(identified as exclusively FW/soil flagellates)
"PHYTORHIZA"Vampyrellids + Phytomyxea + env. novel clade 9(identified as parasites of diatoms and brown algae)
emended FILOSA / CERCOZOAchlorarachniophytes, cercomonads, euglyphids, etc.
environmental novel clade 9parasites of diatoms and brown algae
environmental lineage Endo6(E
NDOM
YXA)
