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Plant Tissue CulturePlant Tissue Culture
SUBMITTED BYASHA R NAIRNATURAL SCIENCE
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What is TissueWhat is Tissue
culture ?culture ?Tissue culture is the ter use! "#r$the %r#cess #" &r#'in& cellsarti(ciall) in the la*#rat#r)+,
Tissue culture in-#l-es *#th %lant an!anial cells
Tissue culture %r#!uces cl#nes. in'hich all %r#!uct cells ha-e the sae
&en#t)%e /unless a0ecte! *) utati#n!urin& culture1
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Whats theWhats the
Bac2&r#un!?Bac2&r#un!?
Tissue culture ha!its #ri&ins at the
*e&innin& #" the34th centur) 'iththe '#r2 #"5#ttlei*
Ha*erlan!t/%lants1 an! Ale6isCarrel /anials1
Ha*erlan!t
Carrel
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The Bac2&r#un!. IIThe Bac2&r#un!. IIThe (rst c#ercial
use #" %lant cl#nal%r#%a&ati#n #narti(cial e!ia 'asin the &erinati#nan! &r#'th #" #rchi!%lants. in the 7834s
In the 7894s an!:4s there 'as a&reat !eal #"research. *ut it 'as#nl) a"ter the!e-el#%ent #" arelia*le arti(cial
e!iu/Murashi&e; S2##&. 78:31 that%lant tissue culturereall)
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The Bac2&r#un!. IIIThe Bac2&r#un!. III
A #re recent a!-ance is the use#" %lant an! anial tissue cultureal#n& 'ith &enetic #!i(cati#n
usin& -iral an! *acterial -ect#rsan! &ene &unst# create&eneticall) en&ineere! #r&aniss
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What is nee!e!?What is nee!e!?
Tissue culture. *#th %lant an! anialTissue culture. *#th %lant an! anialhas se-eral critical re=uireents>has se-eral critical re=uireents>A%%r#%riate tissue/s#e tissues
culture *etter than #thers1
A suita*le &r#'th e!iuc#ntainin&ener&) s#urces an! in#r&anic salts t#su%%l) cell &r#'th nee!s, This can *eli=ui! #r seis#li!
Ase%tic /sterile1 c#n!iti#ns. asicr##r&aniss &r#' uch #re=uic2l) than %lant an! anial tissuean! can #-er run a culture
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What is Nee!e!. IIWhat is Nee!e!. II
5r#'th re&ulat#rs in %lants. *#thau6ins ; c)t#2inins, In anials. thisis n#t as 'ell !e(ne! an! the&r#'th su*stances are %r#-i!e! inseru "r# the cell t)%es #" interest@re=uent su*culturin&t# ensure
a!e=uate nutriti#n an! t# a-#i! the*uil! u% #" 'aste eta*#lites
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Culturin& /icr#%r#%a&atin&1Culturin& /icr#%r#%a&atin&1
Plant Tissue the ste%sPlant Tissue the ste%s Selecti#n #" the
%lant tissue/e6%lant1"r# a health)
-ir#us
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The Ste%s. IIThe Ste%s. II
Esta*lishent#" thee6%lant in a culturee!iu, The e!iusustains the %lantcells an! enc#ura&escell !i-isi#n, It can *es#li! #r li=ui!
Each %lant s%ecies/an! s#eties the
-ariet) 'ithin as%ecies1 has %articulare!iu re=uireentsthat ust *eesta*lishe! *) trialan! err#r
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The Ste%s. IIIThe Ste%s. III
Multi%licati#n Thee6%lant &i-es rise t# acallus/a ass #"l##sel) arran&e! cells1'hich is ani%ulate!*) -ar)in& su&arc#ncentrati#ns an!the au6in/l#'1>c)t#2inin/hi&h1 rati#st# "#r ulti%le sh##ts
The callus a) *esu*!i-i!e! a nu*er#" ties
Di-i!in& sh##ts
Warth an! #! li&ht are essential
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The Ste%s. IThe Ste%s. I
R##t "#rati#nThe sh##ts are
trans"erre! t# a&r#'th e!iu'ith relati-el)hi&herau6in>
c)t#2inin rati#sThe *#ttles #n these rac2sare )#un& *anana %lantsan! are &r#'in& r##ts,
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Tissue culture %lants s#l!
t#
The Ste%s. The Ste%s.
The r##te! sh##tsare %#tte! u%/!eas2e!1 an!
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Wh) !# Plant TissueWh) !# Plant TissueCulture?Culture?
A sin&le e6%lant can *e ulti%lie! int#se-eral th#usan! %lants in less than a)ear this all#'s "ast c#ercial%r#%a&ati#n #" ne' culti-ars
Ta2in& an e6%lant !#es n#t usuall)!estr#) the #ther %lant. s# rare an!en!an&ere! %lants can *e cl#ne!sa"el)nce esta*lishe!. a %lant tissue
culture line can &i-e a c#ntinu#ussu%%l) #" )#un& %lants thr#u&h#ut the)ear
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Wh) !# Plant TissueWh) !# Plant TissueCulture. IICulture. IIIn %lants %r#ne t# -irus !iseases.
-irus "ree e6%lants /ne' eristetissue is usuall) -irus "ree1 can *eculti-ate! t# %r#-i!e -irus "ree %lantsPlant
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Wh) !# Plant TissueWh) !# Plant Tissue
Culture. IIICulture. IIITissue culture all#'s "ast
selecti#n "#r cr#% i%r#-eent e6%lants are ch#sen "r#
su%eri#r %lants. then cl#ne!Tissue culture cl#nes are
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Culturin& Anial TissuetheCulturin& Anial Tissuethe
Ste%sSte%sAnial tissue is
#*taine! either "r# a%articular s%ecien. #r"r# a
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Culturin& AnialCulturin& Anial
Tissue. IITissue. II 5r#'in& the cells Ftissue re=uires an#%tiute%erature. an!
su*culturin& 'henre=uire!Huan cells. "#r
e6a%le are &r#'n
at G!e&rees an!9 C3
Incu*at#r
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Anial tissueFcell culture Anial tissueFcell culture !i0erences "r# %lant tissue!i0erences "r# %lant tissue
culturecultureAnial cell lines ha-e liite! nu*ers #"cell c)cles *e"#re the) *e&in t# !e&ra!eAnial cells nee! "re=uent su*culturin&
t# reain -ia*leTissue culture e!ia is n#t as "ull)
!e(ne! as that #" %lants in a!!iti#n t#in#r&anic salts. ener&) s#urces. ain#aci!s. -itains. etc,. the) re=uire the
a!!iti#n #" seru /*#-ine seru is -er)c##n. *ut #thers are use!1
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Anial tissueFcell culture Anial tissueFcell culture
!i0erences "r# %lant tissue!i0erences "r# %lant tissueculture IIculture IIAnial tissue
cultures can %#se*i#haar!c#ncerns.
an! cultures re=uires%ecial inacti-ati#n'ith h)%#chl#rite/e,&, Jan#la.Chl#r#6.
etc,1 an! thenincinerati#n
The %i%ettes are !is%#sa*le5l#-es an! la*c#at areal'a)s '#rn
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Uses #" Anial TissueUses #" Anial Tissue
CultureCulture5r#'in& -iruses
these re=uire li-in&h#st cells
Ma2in& #n#cl#nalanti*#!ies. use! "#r!ia&n#sis an!research
Stu!)in& *asic cell%r#cesses
5enetic #!i(cati#n; anal)sis
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Uses #" Anial TissueUses #" Anial TissueCulture IICulture II
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Bi*li#&ra%h)Bi*li#&ra%h)
D#!!s. J,H,. R#*erts. L,W,. 7889.E6%erients in Plant Tissue Culture. Gr! e!,.Ca*ri!&e Uni-ersit) PressHartann. H,. Kester. D,. et,al,. 788. Plant
Pr#%a&ati#n. :th e!,. Prentice Hall
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