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7/24/2019 Power Point Prsentation

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Plant Tissue CulturePlant Tissue Culture

SUBMITTED BYASHA R NAIRNATURAL SCIENCE


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What is TissueWhat is Tissue

culture ?culture ?Tissue culture is the ter use! "#r$the %r#cess #" &r#'in& cellsarti(ciall) in the la*#rat#r)+,

Tissue culture in-#l-es *#th %lant an!anial cells

Tissue culture %r#!uces cl#nes. in'hich all %r#!uct cells ha-e the sae

&en#t)%e /unless a0ecte! *) utati#n!urin& culture1


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Whats theWhats the

Bac2&r#un!?Bac2&r#un!?

Tissue culture ha!its #ri&ins at the

*e&innin& #" the34th centur) 'iththe '#r2 #"5#ttlei*

Ha*erlan!t/%lants1 an! Ale6isCarrel /anials1

Ha*erlan!t

Carrel


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The Bac2&r#un!. IIThe Bac2&r#un!. IIThe (rst c#ercial

use #" %lant cl#nal%r#%a&ati#n #narti(cial e!ia 'asin the &erinati#nan! &r#'th #" #rchi!%lants. in the 7834s

In the 7894s an!:4s there 'as a&reat !eal #"research. *ut it 'as#nl) a"ter the!e-el#%ent #" arelia*le arti(cial

e!iu/Murashi&e; S2##&. 78:31 that%lant tissue culturereall)


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The Bac2&r#un!. IIIThe Bac2&r#un!. III

A #re recent a!-ance is the use#" %lant an! anial tissue cultureal#n& 'ith &enetic #!i(cati#n

usin& -iral an! *acterial -ect#rsan! &ene &unst# create&eneticall) en&ineere! #r&aniss


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What is nee!e!?What is nee!e!?

Tissue culture. *#th %lant an! anialTissue culture. *#th %lant an! anialhas se-eral critical re=uireents>has se-eral critical re=uireents>A%%r#%riate tissue/s#e tissues

culture *etter than #thers1

A suita*le &r#'th e!iuc#ntainin&ener&) s#urces an! in#r&anic salts t#su%%l) cell &r#'th nee!s, This can *eli=ui! #r seis#li!

Ase%tic /sterile1 c#n!iti#ns. asicr##r&aniss &r#' uch #re=uic2l) than %lant an! anial tissuean! can #-er run a culture


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What is Nee!e!. IIWhat is Nee!e!. II

5r#'th re&ulat#rs in %lants. *#thau6ins ; c)t#2inins, In anials. thisis n#t as 'ell !e(ne! an! the&r#'th su*stances are %r#-i!e! inseru "r# the cell t)%es #" interest@re=uent su*culturin&t# ensure

a!e=uate nutriti#n an! t# a-#i! the*uil! u% #" 'aste eta*#lites


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Culturin& /icr#%r#%a&atin&1Culturin& /icr#%r#%a&atin&1

Plant Tissue the ste%sPlant Tissue the ste%s Selecti#n #" the

%lant tissue/e6%lant1"r# a health)

-ir#us


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The Ste%s. IIThe Ste%s. II

Esta*lishent#" thee6%lant in a culturee!iu, The e!iusustains the %lantcells an! enc#ura&escell !i-isi#n, It can *es#li! #r li=ui!

Each %lant s%ecies/an! s#eties the

-ariet) 'ithin as%ecies1 has %articulare!iu re=uireentsthat ust *eesta*lishe! *) trialan! err#r


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The Ste%s. IIIThe Ste%s. III

Multi%licati#n Thee6%lant &i-es rise t# acallus/a ass #"l##sel) arran&e! cells1'hich is ani%ulate!*) -ar)in& su&arc#ncentrati#ns an!the au6in/l#'1>c)t#2inin/hi&h1 rati#st# "#r ulti%le sh##ts

The callus a) *esu*!i-i!e! a nu*er#" ties

Di-i!in& sh##ts

Warth an! #! li&ht are essential


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The Ste%s. IThe Ste%s. I

R##t "#rati#nThe sh##ts are

trans"erre! t# a&r#'th e!iu'ith relati-el)hi&herau6in>

c)t#2inin rati#sThe *#ttles #n these rac2sare )#un& *anana %lantsan! are &r#'in& r##ts,


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Tissue culture %lants s#l!

t#

The Ste%s. The Ste%s.

The r##te! sh##tsare %#tte! u%/!eas2e!1 an!


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Wh) !# Plant TissueWh) !# Plant TissueCulture?Culture?

A sin&le e6%lant can *e ulti%lie! int#se-eral th#usan! %lants in less than a)ear this all#'s "ast c#ercial%r#%a&ati#n #" ne' culti-ars

Ta2in& an e6%lant !#es n#t usuall)!estr#) the #ther %lant. s# rare an!en!an&ere! %lants can *e cl#ne!sa"el)nce esta*lishe!. a %lant tissue

culture line can &i-e a c#ntinu#ussu%%l) #" )#un& %lants thr#u&h#ut the)ear


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Wh) !# Plant TissueWh) !# Plant TissueCulture. IICulture. IIIn %lants %r#ne t# -irus !iseases.

-irus "ree e6%lants /ne' eristetissue is usuall) -irus "ree1 can *eculti-ate! t# %r#-i!e -irus "ree %lantsPlant


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Wh) !# Plant TissueWh) !# Plant Tissue

Culture. IIICulture. IIITissue culture all#'s "ast

selecti#n "#r cr#% i%r#-eent e6%lants are ch#sen "r#

su%eri#r %lants. then cl#ne!Tissue culture cl#nes are


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Culturin& Anial TissuetheCulturin& Anial Tissuethe

Ste%sSte%sAnial tissue is

#*taine! either "r# a%articular s%ecien. #r"r# a


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Culturin& AnialCulturin& Anial

Tissue. IITissue. II 5r#'in& the cells Ftissue re=uires an#%tiute%erature. an!

su*culturin& 'henre=uire!Huan cells. "#r

e6a%le are &r#'n

at G!e&rees an!9 C3

Incu*at#r


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Anial tissueFcell culture Anial tissueFcell culture !i0erences "r# %lant tissue!i0erences "r# %lant tissue

culturecultureAnial cell lines ha-e liite! nu*ers #"cell c)cles *e"#re the) *e&in t# !e&ra!eAnial cells nee! "re=uent su*culturin&

t# reain -ia*leTissue culture e!ia is n#t as "ull)

!e(ne! as that #" %lants in a!!iti#n t#in#r&anic salts. ener&) s#urces. ain#aci!s. -itains. etc,. the) re=uire the

a!!iti#n #" seru /*#-ine seru is -er)c##n. *ut #thers are use!1


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Anial tissueFcell culture Anial tissueFcell culture

!i0erences "r# %lant tissue!i0erences "r# %lant tissueculture IIculture IIAnial tissue

cultures can %#se*i#haar!c#ncerns.

an! cultures re=uires%ecial inacti-ati#n'ith h)%#chl#rite/e,&, Jan#la.Chl#r#6.

etc,1 an! thenincinerati#n

The %i%ettes are !is%#sa*le5l#-es an! la*c#at areal'a)s '#rn


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Uses #" Anial TissueUses #" Anial Tissue

CultureCulture5r#'in& -iruses

these re=uire li-in&h#st cells

Ma2in& #n#cl#nalanti*#!ies. use! "#r!ia&n#sis an!research

Stu!)in& *asic cell%r#cesses

5enetic #!i(cati#n; anal)sis


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Uses #" Anial TissueUses #" Anial TissueCulture IICulture II


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Bi*li#&ra%h)Bi*li#&ra%h)

D#!!s. J,H,. R#*erts. L,W,. 7889.E6%erients in Plant Tissue Culture. Gr! e!,.Ca*ri!&e Uni-ersit) PressHartann. H,. Kester. D,. et,al,. 788. Plant

Pr#%a&ati#n. :th e!,. Prentice Hall

Internati#nalhtt%>FF''',une,e!u,auFa&r#n#)FA&SrH#rtT

Cin"#,htlhtt%>FFa&&ieh#rticulture,tau,e!u

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