Hematocrite
Macro-hematocrite
•Wintrobe hematocrite apparatus •3 mm bore- 10 cm graduation
•Double gradation
•Filled with special syringe with blunted end needle .
• Avoid formation of bubbles.
• Ordinary centrifuge 30-60 min.s at 3000 rpm.
• Uses : used to measure both PCV and ESR
Blunt needle used for filling the wintrobe tube with the blood samples
ESR reading
PCV reading
Interpretation
Packed cell layer .(PCV) or (HCT).
35-45 %
Decreased>>>>>indicates anemiaIncreased >>> indicates polycythemia
Buffy coat :
0.5 – 1.2 mmThicker >>>>> leucocytosisThinner >>>>> leucopenia
Pinkish buffy coat indicates…………………………………………
Interpretation
Plasma layer:
• Red >>>>>>
• Intense yellow >>>>>
• Turbid (whitish) >>>>> Lipemia may be post prandial or pathological due to lipid metabolism disorders.
Micro-hematocrite
Micro-hematocrite
Micro-hematocrite
•Special high speed centrifuge•Special reader•5 mins at 15000 rpm.•Cannot interpret diff. layers of hematocrite.
•Advantage:
Less trapping to plasmaLess timeMinimal amount of blood
Exercise:
READING:
33 %
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