Pcv
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Transcript of Pcv
![Page 1: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/1.jpg)
Hematocrite
![Page 2: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/2.jpg)
Macro-hematocrite
•Wintrobe hematocrite apparatus •3 mm bore- 10 cm graduation
•Double gradation
•Filled with special syringe with blunted end needle .
![Page 3: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/3.jpg)
• Avoid formation of bubbles.
• Ordinary centrifuge 30-60 min.s at 3000 rpm.
• Uses : used to measure both PCV and ESR
Blunt needle used for filling the wintrobe tube with the blood samples
![Page 4: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/4.jpg)
ESR reading
![Page 5: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/5.jpg)
PCV reading
![Page 6: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/6.jpg)
Interpretation
Packed cell layer .(PCV) or (HCT).
35-45 %
Decreased>>>>>indicates anemiaIncreased >>> indicates polycythemia
Buffy coat :
0.5 – 1.2 mmThicker >>>>> leucocytosisThinner >>>>> leucopenia
Pinkish buffy coat indicates…………………………………………
![Page 7: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/7.jpg)
Interpretation
Plasma layer:
• Red >>>>>>
• Intense yellow >>>>>
• Turbid (whitish) >>>>> Lipemia may be post prandial or pathological due to lipid metabolism disorders.
![Page 8: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/8.jpg)
Micro-hematocrite
![Page 9: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/9.jpg)
![Page 10: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/10.jpg)
![Page 11: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/11.jpg)
Micro-hematocrite
![Page 12: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/12.jpg)
Micro-hematocrite
•Special high speed centrifuge•Special reader•5 mins at 15000 rpm.•Cannot interpret diff. layers of hematocrite.
•Advantage:
Less trapping to plasmaLess timeMinimal amount of blood
![Page 13: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/13.jpg)
![Page 14: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/14.jpg)
![Page 15: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/15.jpg)
Exercise:
READING:
33 %
![Page 16: Pcv](https://reader036.fdocuments.us/reader036/viewer/2022062405/556ba035d8b42a207e8b464e/html5/thumbnails/16.jpg)
• Watch our videos on cairoVCP channel
www.youtube.com/cairoVCP
• Watch Microhematocrite
http://www.youtube.com/watch?edit=vd&v=-cuukxP3ijI
• www.facebook.com/clinicalpathology