Hematocrite

Macro-hematocrite

•Wintrobe hematocrite apparatus •3 mm bore- 10 cm graduation

•Double gradation

•Filled with special syringe with blunted end needle .

• Avoid formation of bubbles.

• Ordinary centrifuge 30-60 min.s at 3000 rpm.

• Uses : used to measure both PCV and ESR

Blunt needle used for filling the wintrobe tube with the blood samples

ESR reading

PCV reading

Interpretation

Packed cell layer .(PCV) or (HCT).

35-45 %

Decreased>>>>>indicates anemiaIncreased >>> indicates polycythemia

Buffy coat :

0.5 – 1.2 mmThicker >>>>> leucocytosisThinner >>>>> leucopenia

Pinkish buffy coat indicates…………………………………………

Interpretation

Plasma layer:

• Red >>>>>>

• Intense yellow >>>>>

• Turbid (whitish) >>>>> Lipemia may be post prandial or pathological due to lipid metabolism disorders.

Micro-hematocrite

Micro-hematocrite

Micro-hematocrite

•Special high speed centrifuge•Special reader•5 mins at 15000 rpm.•Cannot interpret diff. layers of hematocrite.

•Advantage:

Less trapping to plasmaLess timeMinimal amount of blood

Exercise:

READING:

33 %

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