Microbial Detection Arrays

Interim Review IFebruary 6th, 2007

Aerospace Senior ProjectsUniversity of Colorado – Boulder

Advisors: Dr. Forbes and Dr. MaslanikCustomers: BioServe and Tufts University

Dave MillerElizabeth NewtonTed Schumacher

Shayla StewartSteven To

Charles Vaughan Sameera Wijesinghe

2

Briefing Overview

• Overall Design Summary

• Changes Since CDR

• Test Plans

• Schedule

• Budget

• Current Issues

3

Overall Design Summary

AutoclavesReagent Water Chamber

Peristaltic Pump Mounts

Top Shelf

Environmental ChamberReaction

Chambers (with Mixers)

Mixer Motor Mounts

NOTE: Only shows parts to

be manufactured

4

Changes Since CDR• Mechanical:

– Autoclave: Repositioning of screw holes and O-ring groove– Mixer: Rearrangement of impellers, addition of bottom scoop– Environmental chamber: Walls broken up to simplify

manufacturing and assembly– Shelves and supports: Sizes standardized, COTS supports

• Electrical:– Sensors: LM34 temperature sensors on autoclave, reagent

water chamber, and ambient • PP-0079 thermistors on reaction chamber • Additional thermistors placed on reaction chambers

• Software:– Laptop hard drive for data storage

• Manufacturing:– BioServe manufacturing parts of autoclave bodies (milling),

reaction chamber bodies, and mixers

5

Changes Since CDR

Power Supply Power Distribution

Switch Board

Sensors

Computer

Analog Input

Digital output

Analog Output

Autoclave H1 LED's Autoclave H2

Autolcave TEC 1Autoclave TEC 2

RC Control 1RC Control 2

Mixer Control 1Mixer Control 2

3x 3x 4x 4x 4x 4x

4x2x2x 2x

16x34x

3x

26x

2x

12x

Mixer 2 Mixer 1 RC TEC 2 RC TEC 1 AC TEC 2 AC TEC 1

2x2x2x2x2x2x

Pump1Pump 2

2x2x

Reagent water heater

2x

Mixer Electrical System

Rectangular Mixing Blades

Flat Scoop

Four Controlled Items

6

Test Plan Flow ChartPower Supply

Circuit

Switch BoardCircuit

Peristaltic PumpControl Circuit

AutoclaveHeater

Mixer ControlCircuit

TEC ControlCircuit

IntegratedElectrical

Subsystem

SoftwareInput/Output

Voltage

IntegratedSystem

Verification

AutoclaveTemp/Pressure

SampleTransport

MixingCapability

Reagent H2OContainment

Rxn ChamberEnviron. Control

AutoclaveKill Test

SampleSterility

Disassembly/Reassembly

Electrical

Mechanical

Biological

Software

Integrated

7

Special Test Requirements

• Hazard Analyses– Electrical: electrocution/fire– Autoclave: pressure vessel (projectiles), hot

surfaces, sharp edges– Assembly/Disassembly: sharp edges– Environmental control: hot surfaces– Integrated System: all of above

• Testing Locations– BioServe Environmental Chamber– BioServe Wet Chemistry Lab

8

Schedule

9

Budget

Expected Actual

Electronics $0 $0 (donated)

Sensors $0 $0 (donated)

Mechanical $1,328.53 $1,909.61

Computer/DAQ $348.00 $606.00

Miscellaneous $150.00 $328.01

Total $1,826.53 $2,843.62

10

Current Issues

• Tubing:– PharMed tubing does not come in 1” inner diameter

• Substitution has been found (Bioprene) but has not been ordered

• TEC Controller:– Power cord did not come with TEC controller

• Replacement can be fabricated or purchased

• Manufacturing:– Two weeks behind schedule

• Offset by manufacturing contributions from BioServe

• Ordering:– Pumps, thermostats, and TECs have not been

ordered

11

Conclusions

• Manufacturing behind schedule– Offset by BioServe’s assistance

• Most parts are ordered– Tubing is only ordering concern– Can be offset by finding other distributors

• Still on budget– Over what was expected, but still under budget with

most parts ordered

• Mechanical, software, and electrical packages approved

• Will be able to complete manufacturing and testing by deadlines