Microbial Detection Arrays
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Transcript of Microbial Detection Arrays
Microbial Detection Arrays
Interim Review IFebruary 6th, 2007
Aerospace Senior ProjectsUniversity of Colorado – Boulder
Advisors: Dr. Forbes and Dr. MaslanikCustomers: BioServe and Tufts University
Dave MillerElizabeth NewtonTed Schumacher
Shayla StewartSteven To
Charles Vaughan Sameera Wijesinghe
2
Briefing Overview
• Overall Design Summary
• Changes Since CDR
• Test Plans
• Schedule
• Budget
• Current Issues
3
Overall Design Summary
AutoclavesReagent Water Chamber
Peristaltic Pump Mounts
Top Shelf
Environmental ChamberReaction
Chambers (with Mixers)
Mixer Motor Mounts
NOTE: Only shows parts to
be manufactured
4
Changes Since CDR• Mechanical:
– Autoclave: Repositioning of screw holes and O-ring groove– Mixer: Rearrangement of impellers, addition of bottom scoop– Environmental chamber: Walls broken up to simplify
manufacturing and assembly– Shelves and supports: Sizes standardized, COTS supports
• Electrical:– Sensors: LM34 temperature sensors on autoclave, reagent
water chamber, and ambient • PP-0079 thermistors on reaction chamber • Additional thermistors placed on reaction chambers
• Software:– Laptop hard drive for data storage
• Manufacturing:– BioServe manufacturing parts of autoclave bodies (milling),
reaction chamber bodies, and mixers
5
Changes Since CDR
Power Supply Power Distribution
Switch Board
Sensors
Computer
Analog Input
Digital output
Analog Output
Autoclave H1 LED's Autoclave H2
Autolcave TEC 1Autoclave TEC 2
RC Control 1RC Control 2
Mixer Control 1Mixer Control 2
3x 3x 4x 4x 4x 4x
4x2x2x 2x
16x34x
3x
26x
2x
12x
Mixer 2 Mixer 1 RC TEC 2 RC TEC 1 AC TEC 2 AC TEC 1
2x2x2x2x2x2x
Pump1Pump 2
2x2x
Reagent water heater
2x
Mixer Electrical System
Rectangular Mixing Blades
Flat Scoop
Four Controlled Items
6
Test Plan Flow ChartPower Supply
Circuit
Switch BoardCircuit
Peristaltic PumpControl Circuit
AutoclaveHeater
Mixer ControlCircuit
TEC ControlCircuit
IntegratedElectrical
Subsystem
SoftwareInput/Output
Voltage
IntegratedSystem
Verification
AutoclaveTemp/Pressure
SampleTransport
MixingCapability
Reagent H2OContainment
Rxn ChamberEnviron. Control
AutoclaveKill Test
SampleSterility
Disassembly/Reassembly
Electrical
Mechanical
Biological
Software
Integrated
7
Special Test Requirements
• Hazard Analyses– Electrical: electrocution/fire– Autoclave: pressure vessel (projectiles), hot
surfaces, sharp edges– Assembly/Disassembly: sharp edges– Environmental control: hot surfaces– Integrated System: all of above
• Testing Locations– BioServe Environmental Chamber– BioServe Wet Chemistry Lab
8
Schedule
9
Budget
Expected Actual
Electronics $0 $0 (donated)
Sensors $0 $0 (donated)
Mechanical $1,328.53 $1,909.61
Computer/DAQ $348.00 $606.00
Miscellaneous $150.00 $328.01
Total $1,826.53 $2,843.62
10
Current Issues
• Tubing:– PharMed tubing does not come in 1” inner diameter
• Substitution has been found (Bioprene) but has not been ordered
• TEC Controller:– Power cord did not come with TEC controller
• Replacement can be fabricated or purchased
• Manufacturing:– Two weeks behind schedule
• Offset by manufacturing contributions from BioServe
• Ordering:– Pumps, thermostats, and TECs have not been
ordered
11
Conclusions
• Manufacturing behind schedule– Offset by BioServe’s assistance
• Most parts are ordered– Tubing is only ordering concern– Can be offset by finding other distributors
• Still on budget– Over what was expected, but still under budget with
most parts ordered
• Mechanical, software, and electrical packages approved
• Will be able to complete manufacturing and testing by deadlines