Michael F. Romero, PhD CURRICULUM VITAE
September 2, 2011
Personal Data
Hispanic
Born, Atlanta, Georgia, USA
Dept. Physiology & Bio medical Engineering
Mayo Clinic College of Medicine
200 First St SW, Rochester, MN 55905
Email [email protected]
Tel. 507-284-8127
url: http://mayoresearch.mayo.edu/mayo/research/romero_lab/
http://discoverysedge.mayo.edu/de09-1-kid-romero-hco3transporters/
Education
Undergraduate: Thomas More College, Crestview Hills, KY.
1984 BA: Biology
Associate Degree: Chemistry and Mathematics
Graduate: Case Western Reserve University, Cleveland, Ohio
1984-88 Dept. Developmental Genetics & Anatomy
1988-91 Dept. Genetics (official)
1986-91 Dept. Physiology & Biophysics (lab relocation)
1/1992 PhD October 4, 1991 (CWRU granted PhD Jan. 1992)
Research advisor: Ulrich Hopfer, MD, PhD
Thesis Title: Angiotensin II regulation of ion transport in the rabbit proximal tubule
Professional Experience
11/91-2/92 Post Doctoral Fellow Dept. Physiology & Biophysics,
Case Western Reserve Univ, Cleveland, OH
2/92-7/95 Post Doctoral Fellow Dept. Cellular & Molecular Physiology,
Yale Univ., New Haven CT
7/95-9/97 Associate Research Scientist1
Dept. Cellular & Molecular Physiology,
Yale Univ., New Haven CT
9/97- 6/03 Assistant Professor Dept. Physiology & Biophysics,
Case Western Reserve Univ, Cleveland, OH
10/98-6/03 Assistant Professor Dept. Pharmacology, CWRU
7/03 – 8/31/06 Associate Professor Dept. Physiology & Biophysics, CWRU
7/03 – 8/31/06 Associate Professor Dept. Pharmacology, CWRU
7/1/06 Award of Tenure Case Western Reserve Univ, Cleveland, OH
9/1/06 – present Associate Professor /
Sr. Associate Consultant
4/20/07-present Associate Professor /
Sr. Associate Consultant
Dept. Physiology & Biomedical Engineering
Mayo Clinic College of Medicine
Div. Nephrology & Hypertension
Mayo Clinic College of Medicine
1 Associate Research Scientist is a non-tenure track faculty appointment.
MF Romero Curriculum vitae - September 2, 2011 2
Personal Statement
My background is renal physiology, electrophysiology and ion / solute transporter biophysics. As a postdoctoral fellow with Walter Boron, I used an expression cloning technique with Xenopus oocytes to clone the first cDNA for a Na+/HCO3
- cotransporter (NBCe1, Slc4a4). We have collaborated with Matthias Hediger and several other investigators
to clone and characterize the transport activities of the SLC solute carriers, emphasizing HCO3-, anion and Na+ transport.
Several years ago with Dr. David Mount (Brigham), we cloned and characterized novel electrogenic Cl-/HCO3-
exchangers of the Slc26 family. Since then, we have used human mutations (or cSNPs) to find how function is altered.
We have taken an evolutionary approach to follow our Slc4 and Slc26 genes through evolutionary time, by cloning and expressing ortholog clones particularly from organisms which adapt to unique environments (e.g., euryhaline teleosts, and Diptera insects). Before leaving Yale, I identified and initially characterized a C. elegans Slc4 cDNA (ceNBC, Genbank#AF004926) as an electroneutral Na+/HCO3
- cotransporter (Romero MF, Boron WF. J Am Soc
Nephrol 9: 11A, 1998). In 1998 my lab cloned the first Na+ driven Cl-/HCO3- exchanger (NDAE1) from Drosophila. We
spent several years using this insect clone to understand mammalian transport. In 2000 I help Fei & Leibach characterize currents and H+ transport by 3 C. elegans H+/oligopeptide transporter (J Biol Chem 275: 9563, 2000). We used NDAE1 overexpression in Sf9 cells for membrane protein purification. Unfortunately, this did not work, but we have maintained an interest in structural biology, through modeling (e.g., N-terminus of NBCe1 – JBC 2007) and protein-protein interactions (CaR/Kir4.1/MuppI – AJP 2007, 2009; Slc26a9 with R-region of CFTR – JBC 2009). Recently we have studied transport function of Slc26a6, Slc26a9 and non-mammalian Slc26a5/prestin. Non-mammalian prestins function as Cl-/nHCO3
- exchangers, Cl-/SO42-- exchangers and Cl-/oxalate2- exchangers, allowing models of kidney stones
(oxalate transport) and malaria (Anopheles SO42- transport) to be developed.
We routinely collaborate with laboratories to physiologically characterize ion/solute transporters in Xenopus oocytes (H+ coupled peptide transport, H+ coupled divalent metal transport, H+ coupled folate transport, etc.). Since we normally use glass microelectrodes to determine function of SLC transporters, we are collaborating with Dr. Tian Cui (Mech. Eng., U MN) to biologically verify fabricated, nanosensors to measure ion and solute concentrations. We have collaborated to physiologically characterize HCO3
- transporters (DB Mount, S Hirose, A Kato, P Linser, JA Dow, M
Koelle) and H+ transporters (MA Hediger, F Leibach, B Mackenzie, ID Goldman) as well as other transporters and channels (ME Maguire, RT Miller, K Choe, SJ Russell, J Lieske, VA Lennon, MP Fautsch). These combined projects and approaches allow us to continually hone our transporter expertise. Our current projects use our electrophysiology expertise to physiologically characterize transporters and channel proteins in the kidney, gut and eye. These approaches and tissues are being used to understand the transport and environment of acidosis, kidney stones, glaucoma, GABA- ergic signaling and cystic fibrosis.
Teaching Experience
1981-84 Speech and Debate, Covington Latin High School, Covington, KY
1985-88 Gross Anatomy teaching, CWRU Medical School, Cleveland, OH
Summer ‘93 high school student summer project: electrophysiology
Summer ‘95 supervision of high school biology teacher (APS teacher fellowship):
molecular biology, Xenopus oocyte expression, high school lab development
Summer ‘95 medical student summer project: molecular biology, Xenopus oocyte expression
Summer ‘96 2 Dutch medical students summer project:
molecular biology, Xenopus oocyte expression, electrophysiology
Summer ‘96 2 undergraduates students summer projects (Minority Opportunity Program):
molecular biology (subcloning, PCR), Xenopus oocyte expression, electrophysiology
Summer ’98 SURP-student, Darin Henry (Edinboro U, PA),: Xenopus oocyte expression,
electrophysiology
Summer 2001 SURP-student, Brenda Smith (U Akron, OH): Xenopus oocyte expression, electrophysiology
Fall 2001 Undergraduate intern, Kristin Bartholomew (Edinboro U, PA); molecular biology, bacterial
protein expression
Summer 02-04 SURP-student, Jen DiPiero (Duke): molecular biology, Xenopus oocyte electrophysiology
Summer 03, 05 2003, high school student, Naina Gupta (Schawnee): Western blotting
2005, undergraduate (U Pennsylvania): molecular biology, Xenopus oocyte electrophysiology
Summer 2007 SURF-student, David Ying (MIT): molecular biology, Xenopus oocyte electrophysiology
Summer 2008 SURF-student, Alyona Haritonova (St. Katherine’s): Xenopus oocyte electrophysiology
Summer 2009 SURF-student, Lauren Brin (St. Katherine’s):
Summer 2010 SURF-student, Anna Czapar (Univ Illonois, BME):
Summer 2010 SURF-student, Shannon Newman (Laurence College, Biology):
Summer 2011 SURF-student, Daniel Bondeson (Univ Wisc., Chemistry)
MF Romero Curriculum vitae - September 2, 2011 3
Graduate School Teaching- CWRU
PHOL-532 Molecular Organization of Cells. 10/98 “How to study cells.” 2 hr lecture, 12 graduate and 1 undergraduate students 10/99 “How to study cells.” 2 hr lecture, 14 graduate students
PHOL-460 (Introduction to Molecular Biology.
11/98 “Xenopus oocyte expression,” 1.5 hr lecture, graduate students. 11/99 “Expression Cloning,” 1.5 hr lecture, graduate students. 11/2/00 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. students. 11/6/01 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. students. 11/7/02 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. students. 11/6/03 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. Students (18). (1.5 hr total) 11/04 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. Students (10). (1.5 hr total) 11/05 "Yeast, baculovirus, and oocyte expression; Expression cloning" (1.5 hr total)
PHRM 433 (or Physiology 433) Membrane Transport
1999: 5 graduate students 3/24/99 1 hr lecture + 4/7/99 2 hr discussion (3 hr total)
2000: 4 graduate students 11/14/00 1.5 hr lecture 11/16/00 2 hr discussion (3.5 hr total)
PHRM 435 (Integrative Systems Physiology and Therapeutics, ISPT) Renal Physiology
2001: 15 graduate students 1/17/01 2 hr lecture 1/22/01 2 hr lecture (4 hr total)
2002: 15 graduate students 2/11/02 2 hr lecture
(4 hr total) 2/23/02 2 hr lecture 2003: 2 x 2 hr lectures/discussion as previously (4 hr total) 2004: 2 x 2 hr lectures/discussion as previously (4 hr total) 2005: 2 x 2 hr lectures/discussion as previously 1 x 2 hr original paper discussion (6 hr total)
PHOL 398
1999-2000:
Undergraduate Seminar
co-organizer of Dept Seminar Series.
Weekly
PHOL 468 (Membrane Physiology)
2003, Spring: 2 x 1.5 hr lecture / discussion on “Na+ coupled transporters” (4.5 hr total)
2004, Spring: 5 x 1.5 hr lecture / discussion on “Na+
coupled transporters” (7.5 hr total) Na
+ coupled Cotransporters, other Cotransporters, Exchangers, water transport (thermodynamics & kinetics)
2004, Fall: 5 x 1.5 hr lecture / discussion on “Transporters,” (7.5 hr total) Na
+ coupled Cotransporters, other Cotransporters, Exchangers, Cellular Ion Homeostasis,
water transport (thermodynamics & kinetics) 2005, Fall: 3 x 1.5 hr lecture / discussion on “Transporters,” (4.5 hr total)
Cotransporters, Exchangers, and Regulation of cellular pH
PHOL 476 (Cellular Biophysics)
2005, Spring: 2 x 2 hr lecture / discussion on “Thermodynamics & Cellular compartments” (4 hr total)
PHOL 480 (Organ Systems Physiology)
2002, Fall: Renal Physiology (3 x 1.5 hrs.) (4.5 hr total) Discussion 16 10/21 Introduction; Body Fluid Compartments Discussion 17 10/23 Glomerular Filtration, Tubular Function Discussion 18 10/28 Renal Regulation of Extracellular Fluid Volume
2003: Renal Physiology (3 x 1.5 hrs.) (4.5 hr total) 2005, Spring Renal Physiology (3 x 1.5 hrs.) (4.5 hr total) 2006, Spring Renal Physiology (3 x 1.5 hrs.) + Renal Computer lab (1 x 1 hr)
Pulmonary Acid-Base (1 x 1.5 hrs) (7 hr total)
MF Romero Curriculum vitae - September 2, 2011 4
2003, Feb 26 Expression Cloning 1 hr lecture
Bicarbonate transporter gene family(ies) 1 hr lecture
BCH2021H Molecular aspects of ion transport, Biochemistry at University of Toronto, Toronto, Ontario, Canada
CWRU Dental School Teaching Dental Pharmacology: Renal physiology / pharmacology
(2 hr total)
2002: 60 students (4 hr total) 2/11/02 2 hr lecture 2/23/02 2 hr lecture
CWRU Medical School Teaching Homeostasis I 12/98 - 1/99 1/00
Renal Physiology Renal Physiology
Small groups- 5 x 2 hr meetings "Acid-Base Homeostasis"
2 x 1 hr lectures
(17 hr total)
(18 hr total)
1/01
Renal Physiology
Acid-Base Computer Lab Small groups- 5 x 2 hr meetings "Acid-Base Homeostasis"
1 x 4 hr Lab
3 x 1 hr lectures
3 hr 1/02
Renal Physiology
Acid-Base Computer Lab Small groups- "Acid-Base Homeostasis"
1 x 4 hr Lab 5 x 2 hr meetings 3 x 1 hr lectures
4 hr 10 hr 3 hr
1/03
Renal Physiology
Glomerular filtration Computer Lab Acid-Base Computer Lab Small groups- "Acid-Base Homeostasis"
1 x 2 hr Lab 1 x 3 hr Lab 5 x 2 hr meetings 3 x 1 hr lectures
2 hr 3 hr 10 hr 3 hr
Glomerular filtration Computer Lab Acid-Base Computer Lab Small groups-
1 x 2 hr Lab 1 x 3 hr Lab 5 x 2 hr meetings
2 hr 3 hr 10 hr
(18 hr total) 2/03 GI Physiology Small groups- 2 x 2 hr meetings 4 hr (4 hr total) 2/04 GI Physiology Small groups- 1 x 2 hr meetings 2 hr (2 hr total) 2/05 GI Physiology Small groups- 1 x 2 hr meetings 2 hr (2 hr total) 10/04 Cell Physiology Cystic fibrosis small group 1 x 1 hr meeting 1 hr (1 hr total) 10/05 Cell Physiology Cystic fibrosis small group 1 x 1 hr meeting 1 hr (1 hr total) 11/05 Cardiovascular CV small groups + lab 2x1 + 1x1 5 hr (5 hr total)
Fundamentals of Therupeutics - A (FTA) 1998. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories
1999. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories 2000. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories 2001. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories 2002. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories 3/2003 FTA “Drug elimination” 1 x 1 hr lecture (7 hrs total) 3/2004 FTA Pharmacology lab 2 x 3 hr ferret laboratory (6 hrs total)
Learner College of Medicine at CWRU Teaching
Renal Physiology / Cell Biology Small Group 11/30/2005 Acid-base/epithelial small group, (2 hrs total)
Mayo Foundation Rochester- Graduate School
Course Dates Presentation title / topic Class time required
Transport Physiology Tutorial – BME independent study
Winter 2007 cell and epithelial transport physiology
4 hr / wk (48 hr)
BME 8870 (Course director) Systems Physiology I (Cell Physiology)-
Fall 07 - present (annual course)
principles cellular physiology 3 hrs /wk (36 hr)
MDPD 5150 10/08-present F-grants- Experimental Design 6 hrs total
MMS 3rd - Basic Science Didactic Block/Lecture Series
Winter 2008 Renal Pathophysiology in Sepsis – Fluid Volume”
1.5 hr
BME 8350: Adv. Topics Molec Biophysics 3/08 - present Biophysics of transporters (2 + 2) 4 hr
Core 6300: Molec Biophysics 3/09-present Biophysics of transporters 8 hr
BME 8859 1/09-(as needed) Tutorial in Renal Physiology 2 hr / wk (24-30 hr)
MF Romero Curriculum vitae - September 2, 2011 5
3
-
Mayo Foundation Rochester- Physiology & Biomedical Engineering (BME)
Service Dates Presentation title / topic Class time
required BME oral qualifying exam committee 2006-present Oral examinations 6-7 students/ yr
BME seminar series - 2008- present Topics cellular physiology and
biomedical engineering
2 hr/wk
(weekly)
Medical Scientist Training Program
selection committee
2008-2010 Select and interview MD/ PhD
candidates
BME graduate student interview 2007- present Select and interview PhD candidates for
Biomedical Engineering graduate
program
As required
Postdoctoral Trainees
Trainee Period Subject Current Position Caroline R. Sussman, PhD Instructor, Romero Lab Support: -postdoc fellowship (MS & NRSA)
-Wadsworth Foundation's Young
Investigator Award
-ACES faculty development Award
-AHA Scientist Development Grant
4/97-10/97
6/00- 6/06
Molecular biology CNS & epithelial cell lineages, zebrafish CNS and epithelial development
Assist. Prof., Physiology & BME,
Mayo Clinic College of Medicine
Rochester, MN
Nick Tripoulas Ph.D 6/98- 6/00 Xenopus oocyte expression Postdoc fellow, Cell Biology, Cleveland
Clinic Foundation w/ Dr. Paul Fox
Janina Szewczyk, MD 12/98-2/99 Molecular biology Clinical fellow in LA
Jose Marcelino, PhD 3/99 – 6/00 Oocyte expression Postdoc in Genetics, CWRU
-current: Biotech policy, State of FL
Lamara D. Shrode, PhD 6/99- 8/00 1) NDAE1 in Drosophila, transfected cells and mammals 2) Molecular biology
Scientific wirting, ProEd Communications,
Beachwood, OH
Dorothee Günzel, PhD 8/99- 8/00 Oocyte electrophysiology C3 Prof. Berlin, Germany
Jun-ichi Satoh, MD Postdoc in Romero lab Support: AHA postdoctoral
fellowship (7/01-6/30/03)
4/00- 7/1/03 1) Cloning and localization of mammalian Mg
2+ transporters
2) Functional analysis of human NBCe1 disease mutations
Assist. Prof., Dept. Pharmacology, Jichi
Medical School, 3311-1 Yakushiji
Minamikawachi, Tochigi
JAPAN 329-0498
Zara Josephs, PhD 6/02 – 5/04 1) Cloning, localization and functional characterization of Slc4 & Slc26 HCO
- transporters
in Drosophila and mosquitos 2) developmental localization of zebrafish Slc4 and Slc26 mRNAs
Postdoc in Romero lab
**Young Investigator award for
Comp. Physiol. Section APS, EB 2003
Fellow, Intl Cancer Res. Institute,
London, UK; 6/04- present
Min-Hwang Chang, PhD Support: AHA postdoctoral fellow
(7/1/04-6/30/06)
Natl.AHA-Scientist Dev. Grant
(1/1/10-12/31/13)
Mayo-UMn Partnership grant
(1/1/10-12/31/11)
CWRU fellow
(8/02 – 8/06) Mayo-Ass. Prof
(8/07-present)
1) Structure-function & mutational analysis of NBCe1, the electrogenic Na
+/HCO3
cotransporter 2) Electrophysiology of Slc26a9 3) Characterization of electrogenic Na
+/
monocarboxylate cotransport by Slc5a8
Postdoc in Romero lab (8/02-9/07)
**Young Investigator award for
Cell & Molec. Physiol. Section APS
EB 2003
Assist. Prof., Physiology & Biomed. Eng.
Mayo Clinic College of Medicine
Rochester, MN
Consuelo Plata, PhD **Mexico Independent Grant
(J48709-III)
“Caracterización funcional y
estructural de los transportadores de
sodio monocarboxilatos
(Na+/MCs;TSMC): electroneutro
(TSMCn/Slc5a12) y electrogénico
(TSMCe/ Slc5a8)”
6/03 – 7/31/06 1) Cloning, localization and electrophysiology characterization of zebrafish & mammalian electrogenic & electroneutral Na
+/
monocarboxylate cotransporters
2) Ion transport by Slc26a9
Departamento de Nefrología y Metabolismo
Mineral, Instituto Nacional de Ciencias
Médicas y Nutrición Salvador Zubirán,.
Vasco de Quiroga #15 Col. Sección XVI,
Tlalpan, D.F., México C.P. 14000
-Postdoc in Romero Lab
**Young Investigator award for Cell &
Molec. Physiol. Section APS, EB 2006
MF Romero Curriculum vitae - September 2, 2011 6
Aleksandra Sinđić Ph.D., M.D. Support: Cystic fibrosis foundation
postdoctoral fellow (5/06-4/08)
Wasantha K. Ranatunga, PhD
1/05 – 8/31/07 1) Immunolocalization of Slc26a9 and Slc5a8 2) Functional interaction of Slc26a9 and CFTR
7/07- present 1) STAS - expression &
purification 2) SLC crystalization
Postdoc in Romero Lab
9/1/07
Assist. Prof., Dept. Physiology
Univ. Zagreb
Zagreb, Croatia
Postdoc in Romero Lab
AnPng Chen, PhD 9/07 – present 1)Elasmobranch SLC transporters 2)SLC26A9 – SNP function
Postdoc in Romero Lab
Taku Hirata, PhD DK083007-z supplement
11/09 – present 1) Slc26 oxalate transport 2) Drosophila model of stones 3) Slc26 structure
Postdoc in Romero Lab
Merfake H. Semret, MD 11/09- 11/10 Nephrology & Hypertension fellow
Doctoral Trainees
Trainee Period Role / Subject Current Position
Christopher M. Sciortino 12/97-4/30/01 Thesis Advisor:
-electrophysiology, molec biology
”Characterization & localization of
the sodium mediated bicarbonate
transporters NBC & NDAE1”
PhD, CWRU, 2001
MD, CWRU – 2005
Surgery Residency
Johns Hopkins, 2005 - present
Theresa Fagan 5/99-7/99 Rotation supervisor:
Oocyte electrophysiology CWRU, Ph.D. 2003- Cell Physiology
(A. Scarpa / A. Romani)
Jane Kim 6/04-7/15/04 Rotation supervisor:
Oocyte electrophysiology PhD program – Cell Physiology
Andreas Fulterer 12/09 – 5/2010 Visiting PhD student:
Transporter biophysics
Oocyte electrophysiology
-microelectrodes
-patch clamping
Universität Stuttgart, Stuttgart, Germany
Yusuke Ito 12/10-4/11 Visiting PhD student:
Transporter biophysics
Oocyte electrophysiology
-microelectrodes
-patch clamping
Biological Sciences, Tokyo Tech, Japan
(S. Hirose lab)
Thesis Committees
Trainee Period Role / Subject Current Position Christopher M. Sciortino, PhD
12/97-4/30/01 Thesis Advisor
-electrophysiology
-molecular biology
PhD Biophysics, 2001; MD, CWRU 2005 Surgery Residency
Johns Hopkins, 2005 - present Joanne Nagy, PhD 6/98-9/14/01 Thesis Committee member Ph.D. - Cell Physiology, (C. Sanders) Kirill Oxenoid, Ph.D. 3/99-12/26/02 Thesis Committee Chair Ph.D. – Biophysics (C. Sanders) Daw-Yang (Darwin) Hwang, MD, PhD
4/99-6/02 Thesis Committee Chair Ph.D. - Cell Physioilogy (F. Ismail-Beigi) -Cardiology Fellow – Tiawain
Lalitha Gudipaty, PhD 5/99-5/03 Thesis Committee member Ph.D.-Cell Physiology (G.Dubyak) Medical student – CWRU
John W. Vince, PhD 2000 External Reviewer: Univ. Toronoto,
Dept. Biochemistry
Ph.D.– Biochemistry, (R.A.F. Reithmeier) "Interaction of Chloride/Bicarbonate Anion Exchangers with Carbonic Anhydrase II"
Brian Wisnoskey, PhD 2001- 2004 Thesis Committee member Ph.D. - Cell Physiology (W. Schilling) Joydeep Sarkar 2002 – present Thesis Committee member Ph.D. candidate–Biomedical Eng, CWRU
(P. Fox – Clev. Clinic Found.) Steve Radabaugh, MS 2003- 7/04 Thesis Committee member M.S. - Cell Physiology (C. Smith) Nichol Baitz, MS 2003-5/05 Thesis Committee member M.S. - Cell Physiology (A. Romani) Suzanne McAlear 2003- 1/07 External Committee Member: Ph.D. candidate @ U AL, Birmingham
Physiology & Biophysics
MF Romero Curriculum vitae - September 2, 2011 7
(M.O. Bevensee-advisor) Shiyuan Ryan Hong 2004-present Thesis Committee member Ph.D. candidate - Cell Physiology
(G. Dubyak) Rebecca Falin 2004-present
2006 defense
Thesis Committee member
Ph.D. candidate - Cell Physiology
(C. Cotton)
Vanderbilt Univ (K. Strange)
Aaron Brister 2005-10/2006 Thesis Committee member
Left program
Ph.D. candidate - Cell Physiology (C. Cotton)
Lizette Warner 11/2006-12/08 Thesis Committee member PhD candidate – Mayo BME (J.C. Romero)
Peter Conrad 1/09-present Thesis Committee member MSTP candidate – Mayo BME (S. Sine)
Carolina Amador Carrascal 11/08-present Thesis Committee member PhD candidate – Mayo BME (J. Greenleaf)
John R. Giudicessi 11/09-present Thesis Committee member MSTP candidate – Mayo Biochem Molec Biol. (M. Ackerman)
Undergraduate / Other trainees
Trainee Period Subject Current Position
Dustin M. Paz 6/96-8/96 molecular biology (subcloning, PCR),
Xenopus oocyte expression,
electrophysiology
Susan Elsby 6/96-8/96 molecular biology, Xenopus oocyte
electrophysiology
MD- Harvard Univ
Resident-surgery UCSF
Unknown
Darin Henry (Edinboro U) 6/98-8/98 Electrophysiology Pharmacia Corporation, CT
Brenda L. Smith (U Akron) 6/01-9/01 Electrophysiology Applying for Medical Technolgy
Kristin Bartholomew
(Edinboro U)
9/01-12/31/01 Molecular biology Unknown
Jennifer DiPiero (Duke) 5/02-8/02;
6/03 - 8/15/03
6/04 – 8/04
-Molecular biology / electrophys.
-zebrafish molecular biology
-
BA-Duke Univ. (5/04)
-Duke Genetics lab (9/04-7/05)
-OH State Dental School (8/05 – 5/09)
-Dental practice OH
Naina Gupta (Schawnee
High School, Lima, OH)
(U. Penn)
Mayo Clinic
6/03 – 8/15/03
6/05 – 8/05
-Molecular biology, immunohisto.
-Molecular biology, electrophys. High school student
U. Penn – Biology Major
Medical School
David Ying (MIT) Summer 07 -Molec. biology (Slc26a9), electrophys. Washington Univ. School of Medicine
(MIT – BME major, 2009)
Alyona Horitonova (St.
Katherine’s, St. Paul, MN)
Summer ‘08 Slc26a2 8/09:Univ MN, Biomedical Eng. (PhD)
(St. Katherine – Physics major, 2009)
Lauren Brin (St. Katherine’s,
St. Paul, MN)
Summer ‘09 -molecular biology (insect Slc26 clones) -electrophysiology
St. Katherine – Chemistry major
8/10-present: U Nebraska Med Sch
Anna Czapar (soph) Summer ‘10 -molecular biology (insect Slc26 clones) -electrophysiology -silkworm protein expression
Shannon Newman (jr) Summer ‘10 -molecular biology -electrophysiology -protein purification and crystalization
Dan Bondeson (jr) Summer ‘11 -Drosophila genetics -electrophysiology (VC & [Cl-]) - tubule YFP fluorescsence
Univ Illinois (Biomedical Engineering)
-applying to MSTP programs
Laurence Univ (Biology)
-8/11: U Wash (Biochemistry)
U WI- Stevens Point (Chemistry)
-applying to grad school
MF Romero Curriculum vitae - September 2, 2011 8
Colleagues / Sabbaticals
Trainee Period Subject Current Position
Daniel Markovich, PhD
Sabbatical
9/02-1/03 Xenopus oocyte electrophysiology of
Slc26 transporters
Associate Professor, Physiology
Univ. Queensland, Brisbane, Australia
**Human Frontiers Science Program, short-term
fellowship for sabadical
Mayo Clinic
Akira Kato, PhD
Sabbatical 7/15-11/07
3/15-4/15/08
10/10–12/10
Xenopus oocyte electrophysiology
Slc26a6 transport
Fugu NHE3 / MCT transport 2+
Assistant Professor, Biological Sciences
Tokyo Institute of Technology,
4259 Nagatsuta-cho, Midori-ku
8/11-10/11 Fugu Mg transport Yokohama-shi, Kanagawa-ken, Fugu borate transport 226-8501 JAPAN
Awards / Honors
1980 Thomas More College, 4 year scholarship St. Xavier University, partial 4 year scholarship
1982 Health Careers Opportunity Program Participant: Univ. Kentucky, Lexington, KY
1983 Summer Research Fellowship: Dept. Biochemistry, Univ. Louisville, Louisville, KY (Research supervisor: Robert Grey, PhD)
1984-86 Graduate Fellowship: Dept. Developmental Genetics & Anatomy, Case Western Reserve University
1986-89 NRSA predoctoral fellowship #HL-07415-08
1991 Travel Award for 1991 ASCB Annual Meeting in Boston, American Society for Cell Biology Minority Affairs Committee
1992 Postdoctoral fellowship (training grant) #DK-07259-15
7/93-7/95 National Kidney Foundation Research Fellowship
1994 a) Am. Physiol. Soc. NIDDK Travel Award, FASEB
b) Am. Physiol. Soc., Gastrointestinal Physiology: Postdoctoral Research Award, FASEB
c) Am. Physiol. Soc., Renal Physiology: Postdoctoral Research Award, FASEB
8/95-8/98 NRSA postdoctoral fellowship #1-F32-DK09342-01
1996 a) Caroline tum Suden/Frances A. Hellebrandt Professional Opportunity Award (APS)
b) Accommodation Award, Society of General Physiologists Annual Meeting
1997 ISN. Travel Grant , XIVth
International Congress of Nephrology, Sidney, May 25-29, 1997
1998 CWRU nominee for Pew Scholar Award
2000 Am. Physiol. Soc., Renal Section: New Investigator Travel Award
2001 a) Cover art- Am. J. Physiol. Cell Physiol. July – Dec 2001
b) Mary Jo Cosio, RN Research Award - National Kidney Foundation of Ohio
2002 APS Careers in Physiology brochure – one of five physiologists featured
2003 APS New Investigator- Cell & Molecular Physiology Section
2006 Tenure, Case Western Reserve University School of Medicine
MF Romero Curriculum vitae - September 2, 2011 9
3
3
-
-
Support: (Completed)
8/1/95-7/31/98 F32-DK009342 (PI:Romero MF) [priority: 146; 19 %-ile]
Expression cloning of Na/HCO3 cotransporter
1/1/97-12/31/00 Am. Heart Assoc. Scientist Development Grant (# 9630322N) (PI: Romero, 30%)
"Physiology, Localization, and Regulation of the renal Sodium/Bicarbonate Cotransporter, NBC"
[total direct = $260,000]
1/1/98-12/31/98 Ireland Cancer Center: CWRU and University Hospitals (ACS-IRG-91-022-05-IRG)
Role of Na/HCO3 Cotransporters, NBC, in Prostate Cancer
4/1/99-3/31/00 CWRU/UH Cystic Fibrosis Core Center Feasibility Grant (PI: Romero, 5%)
"Role of Na/HCO3 Cotransporters, NBC, in the Pancreatic HCO–
Transport"
7/1/00-6/30/02 National Kidney Foundation of Ohio (PI: Romero)
" Cloning and expression of a mammalian Mg2+
transporter " [total direct: $25,000)
7/01/02-6/30/04 NIH-R21-DK60845 (PI: MF Romero) 10%
"HCO–
transporters in Drosophila and mosquitoes" [total direct: $200,000]
5/1/00-4/30/06 NIH-R01-DK56218 (PI: Romero, 50%) [priority: 145; 5.7 %-ile]
"Characterization of the Na+
driven anion exchanger, NDAE1" [total direct: $986,400]
12/01/01-11/30/06 NIH / NIGMS R01-GM61748 (PI: ME Maguire) Romero, 5%
“NRAMPs, Manganese and Salmonella Virulence” [total direct: $750,000)
4/1/06 – 3/31/08 Cystic Fibrosis Foundation, Research Grant (PI: Romero, ROMERO06G0)
"Slc26a9, a novel electrogenic, Na+
transporting Cl/HCO3 exchanger in lung" [direct = $110,000]
(Active)
9/1/06 – 8/31/11 R01-EY017732 (PI: Romero, 30%) [priority: 163; 17.5 %-ile];
"Molecular mechanisms of the electrogenic Na+
Bicarbonate Cotransporter (NBCe1/Slc4a4)"
[total direct = $1,125,000]
7/01/08-6/30/13 R01-CA100634-06 (PI: SJ Russell; Romero 2%); “Radiovirotherapy for multiple myeloma”
9/1/08-8/31/13 P50-DK083007 (PI: Lieske; Admin Core, Romero, 5%)
Mayo Clinic Urology O’Brien Center Research
8/09-7/13 NIH-P30DK084567 [PI: N LaRusso] (Romero MF, associated investigator)
Mayo Clinic Center for Cell Signaling in Gastroenterology
1/1/10-12/31/11 Mayo-UMn Partnership; (PIs: M-H Chang / MF Romero, Mayo; T Cui, UMn)
“Low cost and high performance nano-ionsensors”
9/30/10-9/29/15 P30-DK090728 - Mayo Translational PKD Center (PI: VE Torres)
Model Systems Core B (PI: Romero, 10%)
10/4/10-9/30/11 Mini-sabbadical supplement from NIH-P30DK084567 [PI: Romero MF]
Drosophila model of oxalate stones – proteomics & knockdowns
12/1/10-11/30/12 P50-DK083007-Training Supplement (Romero / Lieske co-PI’s)
Summer Research Fellowships in Urology Research
12/1/11 – 11/30/16 R01-EY021727-01 (MPI) (Fautsch MP, Opthamol / Romero MF – Physiol & BME)
Intraocular pressure regulation via ATP-sensitive potassium channels
(Pending)
(submitted but not funded)
7/1/09-6/30/11 AHA-Grant in Aid: OXALATE transport, binding and Slc26 structure
3/1/09-2/28/10 Bill and Melinda Gates Foundation (PI: Romero)
10/1/10-9/30/14 1R01GM095594-01 (MPI) (PI: Romero MF/ Thompson JR)
“Novel low-cost expression of functional membrane proteins in silkworms”
1/1/11-12/31/14 R01-NS066040-01A2 (MPI) (Koelle MR, Yale / Romero MF, Mayo)
"Chloride transporters that regulate inhibitory neurotransmission"
2/1/11-1/31/13 Oxalosis and Hyperoxaluria Foundation [Romero MF-PI]
“Drosophila model of oxalate nephrolithiasis”
4/1/11 – 3/31/16 R01-EY021727 (MPI) (Fautsch MP, Opthamol / Romero MF – Physiol & BME)
Intraocular pressure regulation via ATP-sensitive potassium channels
7/1/11-6/30/16 R01 DK092408-01 (MPI) [Romero MF, Mayo / Dow JAT, U Glasgow]
“Drosophila model of oxalate nephrolithiasis”
9/1/11-8/31/16 Competitive renewal of R01-DK56218-06A1 (PI: Romero, 30%)
"Slc26a9, a novel electrogenic, Na+ transporting Cl/HCO3 exchanger "
MF Romero Curriculum vitae - September 2, 2011 10
Training Grants – Trainer (Previous @ Case)
9/91 – 8/06 NIH T32-DK007678 (PI: U. Hopfer); Cell Physiology Training Program
7/94 – 5/09 NIH (T32-HL007415) (PI: P.B. Davis); Cystic Fibrosis Research Training Program
7/01 – 6/06 NIH (T32-GM008803) (PI: P.N. MacDonald);
Predoctoral Training Program in Molecular Therapeutics Training Grants – Trainer (Mayo Clinic): (Active)
7/75-11/07 NIH T32DK007013 (PI: VE Torres); - Kidney Disease Research Training Program
7/03-6/13 NIH T32GM065841 (PI: G. Isaya); Medical Scientist Training Program at Mayo Clinic
Mentoring Grants for Trainees: (Previous)
7/1/01 – 6/30/03 American Heart Association- Ohio Valley affiliate PI: Dr. Jun-ichi Satoh
10/1/02 – 12/31/02 Human Frontiers Science Program- short-term fellowship PI: Dr. Daniel Markovich
7/1/04 – 6/30/06 American Heart Association- Ohio Valley affiliate PI: Dr. Min-Hwang Chang
5/1/06 – 4/30/08 Cystic Fibrosis Foundation, postdoctoral fellowship PI: Dr. Aleksandra Sinđić (SINDIC06F0)
(Current / Funded)
1/1/10-12/31/13 AHA-SDG, National PI: Min-Hwang Chang)
1/1/10-12/31/11 Mayo-UMn Partnership; (PIs: M-H Chang / MF Romero, Mayo; T Cui, UMn)
“Low cost and high performance nano-ionsensors”
1/1/10-11/30/10 P50- DK083007z (P50 PI: Lieske; Pilot PI: Taku Hirata);
“Analysis of kidney stone disease using Drosophila genetic model”
Professional Activities:
Memberships:
1984-1993 American Chemical Society
1991-present American Society for Cell Biology
1992-present American Association for the Advancement of Science
1996-present American Physiological Society
1998- present American Society of Nephrology
2007-present American Society of Biochemistry and Molecular Biology (ASBMB)
Editorial Boards:
2000-2003 Am. J. Physiol. - Gastrointestinal & Liver Physiol.
2001-present Am. J. Physiol. Renal Physiol
2004 Advances in Physiology Education – guest Editor for “Cellular Homeostasis”
7/1/07-6/30/12 J. Biological Chemistry
Professional Service:
1997- present ad hoc reviewer
American Journal Physiology (AJP): Renal; AJP: Cell; AJP: GI; AJP: Heart, AJP: Comp & Reg
Physiol,Cancer Research; Current Eye Research; Archives of Biochemistry and Biophysics;
Molecular Endocrinology; Mammalian Genome,; J. Biol. Chem.; PLoS-Biology; Proc. Natl. Acad.
Sci, USA. , J. Am. Soc. Nephrol., Nature, J Cell Physiol., J Gen Physiol., J Physiol. J. Membr.
Biol., FEBS Letters, Insect Molecular Biology, Human Mutation, Human Molecular Genetics
July 1998 MiniSymposium and poster session organiser for Membrane Transport Proteins-Gordon
Conference, Tilton, NH, July 19-24, 1998
1/1/00-12/31/02 American Physiology Society-Education Committee (elected by APS Council)
2000 VA grant review, National Science Foundation ad hoc reviewer
2000-2004 APS-Renal Section abstract reviewer
2000 Am. Soc. Nephrol- abstract reviewer: Cell and Transport Physiology-Acid-Base
2001 Experimental Biology 2001 Symposium Organizer / Chair – APS-Renal section & Epithelia group
"Molecular mechanisms of bicarbonate transport."
2002-2004 Cell Physiology Study Section 4, American Heart Association, Ohio Valley / Southern Affiliate
*2002 NIH ad hoc reviewer, General Medicine B (GMB) Study Section (Oct 2002)
*2003 NIH ad hoc reviewer, Molecular, Cellular, & Developmental Neuroscience Study Section
(MDCN4) Study Section (Jan 2003)
2003 Experimental Biology 2003 Feature Topic Organizer / Chair- APS-Cell section:
MF Romero Curriculum vitae - September 2, 2011 11
"Molecular physiology of bicarbonate transporters."
5/2003 APS rep and judge for Intel International Science and Engineering Fair, Cleveland, OH
*2002-2005 International Union of Physiology Sciences (IUPS) Renal section advisory board for
2005 IUPS meeting scientific program:
*2004 Experimental Biology 2004 Teaching Symposium Organizer / Chair- APS-Education Comm.:
"Cellular homeostasis."
1/1/04-12/31/06 American Physiology Society-Communications Committee (elected by APS Council)
1/1/04 -12/31/06 American Physiology Society-Epithelial Transport Group steering committee (elected)
2005 IUPS / Exp. Biol. 2005 Feature Topic (#37) organizer / Chair: “Acid-base transporters”
*2005-2009 International Union of Physiology Sciences (IUPS) Renal section advisory board for
2009 IUPS meeting scientific program (Kyoto, Japan)
2006-2009 Vice Chair, APS – Cell and Molecular Physiology section (elected)
7/2006-6/2010 NIH study section member, Cellular & Molecular Biology of the Kidney (CMBK)
2006 NSF-ad hoc reviewer ; Deutsche Forschungsgemeinschaft – ad hoc reviewer
2007 BioMedical Transporters 2007 conference (Berne, Switzerland) - “Structure of transporters” Chair / Organizer;
“Plasma membrane transporters: importance in diseases, drug development and drug toxicity” – Co-Chair
2009-2012 Vice Chair, APS – Cell and Molecular Physiology section (elected, 2nd
term)
7/2010 NIDDK Special Emphysis P01 panel
12/2010 NIDDK (ZDK1 GRB-G J2 1) – Training grants + K-grants
CWRU - Professional Service:
4/4/03 Judge for “ShowCase” at CWRU
2004- present Steering Committee, Post Baccalaureate Research Education Program (PREP)
School of Medicine - Professional Service:
1997- present BSTP Admissions Committee: interviewing of graduate student candidates
1998 – present SOM Year 1 teaching – Homeostasis
1998 – 2004 SOM Year 1 teaching – Fundamentals of Therupeutics
2000 Research America representative from Physiology & Biophysics
5/05-5/07 Physiology & Biophysics representative to Case School of Medicine Faculty Council
1/06 – 8/31/06 Member, Vision Science Research Center (http://www.caseeyes.com/index2.html)
Physiology & Biophysics - Professional Service:
1997- present Dept. Admissions Committee: interviewing of graduate student candidates
1998-99 Dept. Seminar Series Co-ordinator
1998 – present graduate student teaching- see Teaching
1998-present Graduate student thesis committees
2002 - 2005 Organizer of Dept. Postdoctoral Recruitment & Mentoring
2003-2005 DPB-web page committee
Pharmacology - Professional Service:
1997- present Dept. Admissions Committee: interviewing of graduate student candidates
1998 – present graduate student teaching- see Teaching
1998-present Graduate student preliminary exam committees
Mayo - Professional Service:
2006-present BME prelim exam committee
2008-present MD-PhD admissions committee
2009-present Research Space, Equipment and Capital Allocation Subcommittee Patents / PCT applications:
5/20/97 filed PCT International application, 44574-5020-WO (amended 5/20/98, currently pending)
NBC, a gene that encodes a member of the bicarbonate transporter family of proteins
2/18/00 filed PCT (pending) "NDAE1, a new bicarbonate transporter."
2/28/03 filed PCT (pending) “Cloning and characterization of SLC26A7 and SLC26A9 anion exchangers”
124/50/3 PCT/US (with Vanderbilt U. and Brigham & Women’s Hospital).
MF Romero Curriculum vitae - September 2, 2011 12
3
3
3
3
-
3
Publications: (Papers)
1. Douglas, JG, M Romero, and U Hopfer. Signaling mechanisms coupled to the angiotensin receptor of proximal tubule
epithelium. Kidney Int 38(Supp. 30): S43-S47, 1990. [3 citations]
2. . [33 citations]
3. Romero, MF, JG Douglas, RL Eckert, U Hopfer, and JW Jacobberger. Development and characterization of rabbit
proximal tubular epithelial cell lines. Kidney Int 42: 1130-1144, 1992. [34 citations]
4. Andreatta-van Leyen, S, MF Romero, MC Khosla, and JG Douglas. Modulation of phospholipase A2 activity and sodium
transport by angiotensin (1-7). Kidney Int 44: 932-936, 1993. [66 citations]
5. Gorodeski, GI, MF Romero, U Hopfer, E Rorke, WH Utian, and RL Eckert. Human uterine cervical epithelial cells
grown on permeable support:a new model for the study of differentiation. Differentiation 56: 107-18, 1994. [52 citations]
6. Gorodeski, GI, U Hopfer, RL Eckert, WH Utian, BJ De Santis, EA Rorke, and MF Romero. Extracellular ATP decreases
accutely and reversibly transepithelial transport through the paracellular pathway in human uterine cervical cells. Am J
Phsyiol 266: C1692-C1698, 1994. [12 citations]
7. Fei, Y-J, Y Kanai, S Nussberger, V Ganapathy, FH Leibach, MF Romero, SK Singh, WF Boron, and MA Hediger.
Expression cloning of a mammalian proton-coupled oligopeptide transporter. Nature 368: 563-566, 1994. [600 citations]
8. Gorodeski, GI, D Merlin, BJ De Santis, KA Frieden, U Hopfer, RL Eckert, WH Utian, and MF Romero. Characterization
of paracellular permeability in cultured human cervical epithelia: regulation by extracellular adenosine triphosphate. J
Soc Gynecol Invest 1: 225-233, 1994. [19 citations]
9. Kanai, Y, S Nussberger, MF Romero, WF Boron, SC Hebert, and MA Hediger. Electrogenic properties of the epithelial
and neuronal high affinity glutamate transporter. J Biol Chem 270(28): 16561-8, 1995. [128 citations]
10. Steel, A, S Nussberger, MF Romero, WF Boron, CAR Boyd, and MA Hediger. Stoichiometry and pH-dependence of the
mammalian proton-dependent oligopeptide transporter PepT1. J. Physiol. 498(3): 563-569, 1997. [68 citations]
11. Nussberger, S, A Steel, D Trotti, MF Romero, WF Boron, and MA Hediger. Symmetry of H+
binding to the intra- and
extracellular side of the H+
-oligopeptide cotransporter PepT1. J Biol Chem. 272(12): 7777-7785, 1997. [42 citations]
12. Romero, MF, MA Hediger, EL Boulpaep, and WF Boron. Expression cloning and characterization of a renal electrogenic
Na+/HCO
– cotransporter. Nature 387: 409-413, 1997. [296 citations]
13. Gunshin, H, B Mackenzie, UV Berger, Y Gunshin, MF Romero, WF Boron, S Nussberger, JL Gollan and MA Hediger.
Expression cloning and characterization of a mammalian proton-coupled divalent cation transporter. Nature 388: 482-487,
1997. [1713 citations] PMID- 9242408
14. Nakhoul, NL, BA Davis, MF Romero and WF Boron. Effect of expressing the water channel aquaporin-1 on the CO2
permeability of Xenopus oocytes. Am. J. Physiol. 274:C543-C548, 1998. [174 citations]
15. Romero, MF, P Fong, UV Berger, MA Hediger, and WF Boron. Cloning and functional expression of rNBC, an
electrogenic Na+-HCO– cotransporter from rat kidney. Am. J. Physiol. 274:F425-F432, 1998. [123 citations]
16. Schmitt, BM, D Biemesderfer, EL Boulpaep, MF Romero, and WF Boron. Immunolocalization of the Electrogenic Na+/
HCO–
Cotransporter in Mammalian and Amphibian Kidney. Am. J. Physiol. 276: F27-F36, 1999. [121 citations]
17. Choi I, MF Romero, Khandoudi, N. A Bril, and WF Boron. Cloning and characterization of an electrogenic Na/HCO3
cotransporter isoform from human heart (hhNBC). Am. J. Physiol. 276: C576-584, 1999. [91 citations]
18. Heyer, M., Müller-Berger, S., Romero, M.F., Boron, W.F., Frömter, E. Stoichiometry of rat kidney Na-HCO3
cotransporter expressed in Xenopus laevis oocytes. Pflügers Arch. 438: 322-329, 1999. [39 citations]
19. Sciortino CM and MF Romero. Cation and voltage dependence of the rat kidney, electrogenic Na+/HCO
– cotransporter,
rkNBC, expressed in Xenopus oocytes. Am. J. Physiol., 277: 611-623, 1999. [57 citations]
20. Roussa E, Romero MF, Schmitt BM, Boron WF, Alper SL and Thévenod F. Immunolocalization of AE2 anion exchanger
and Na+-HCO3 cotransporter in rat parotid and submandibular glands. Am. J. Physiol. 277: G1288-96, 1999. [37 citations]
21. Thévenod, F., Roussa, E., Schmitt B. M., and Romero M. F. Cloning and immunolocalization of a rat pancreatic Na+
bicarbonate cotransporter. Biochem. Biophys. Res. Comm. 264: 291-298, 1999. [38 citations]
22. Bevensee, M. O., Schmitt, B. M., Choi, I., Romero, M. F., Boron, W. F. An electrogenic Na/HCO3 cotransporter (NBC)
with a novel C terminus, cloned from rat brain. Am. J. Physiol. Cell Physiol. 278:C1200-1211, 2000. [67 citations]
23. Fei Y-J, MF Romero, M Krause, J-C Liu, W Huang, V Ganapathy, and FH Leibach. A novel H+ symporter, OPT3 from
C. elegans. J. Biol. Chem. 275: 9563-9571, 2000. [18 citations]
24. Grichtchenko, I. I., Romero, M. F., Boron, W. F. Extracellular HCO–
dependence of electrogenic Na/HCO3
cloned from salamander and rat kidney. J. Gen. Physiol 115: 533-546, 2000. [27 citations]
cotransporters
25. Romero MF, D Henry, S Nelson, PJ Harte, AK Dillon and CM Sciortino. Cloning and characterization of a Na+
driven
anion exchanger (NDAE1): a new bicarbonate transporter. J. Biol. Chem. 275: 24552-9, 2000. [66 citations]
26. Sciortino, CM, LD Shrode, BR Fletcher, PJ Harte, MF Romero. Localization of Endogenous and Recombinant Na+-driven
Anion Exchanger Protein, NDAE1, from Drosophila melanogaster. Am. J. Physiol. Cell Physiol. 281: C449-463, 2001.
[15 citations]
MF Romero Curriculum vitae - September 2, 2011 13
27. Ducoudret, O, A Diakov, S Mueller-Berger, MF Romero, and E Frömter. The renal Na-HCO3-cotransporter expressed in
Xenopus laevis oocytes: Inhibition by tenidap and benzamil and effect of temperature on transport rate and stoichiometry.,
Pflügers Arch. 442: (5) 709-717, 2001 [5 citations]
28. Marcelino, J., CM Sciortino, MF Romero, LM Mink, RT Ballock, AN Economides, PM Eimons, RM Harland, ML
Warman. Human disease-causing NOG missense mutations: effects on noggin secretion, dimer formation, and BMP
binding. Proc. Natl. Acad. Sci, USA. 98 (20): 11353-8, 2001. [42 citations]
29. Xie, Qizhi, Rick Welch, Adriana Mercado, Michael F. Romero, and David B. Mount. Cloning of the SLC26A6 anion
exchanger, functional comparison to SLC26A1. Am. J. Physiol. Renal Physiol. 283: F826-838, 2002. [104 citations]
30. Flagg, Thomas P., Dana Yoo, Christopher M. Sciortino, Margaret Tate, Michael F. Romero, Paul A. Welling. Molecular
Mechanism of a COOH-terminal Gating Determinant in the ROMK Channel Revealed by a Bartter’s Disease Mutation J.
Physiol. 544 (2): 351-362, 2002. [13 citations]
31. Hirata Taku, Toyoji Kaneko, Toshihiro Ono, Takeru Nakazato, Norihisa Furukawa, Sanae Hasegawa, Shigeo
Wakabayashi, Munekazu Shigekawa, Min-Hwang Chang, Michael F. Romero, Shigehisa Hirose. Mechanism of acid
adaptation of a fish living in a pH 3.5 lake. Am J Physiol Regul Integr Comp Physiol (January 16, 2003). 284: R1199-
R1212, 2003. [95 citations]
32. Li Hui, Lois Myeroff, Dominic Smiraglia, Michael F. Romero, Theresa Pretlow, Lakshmi Kasturi, James Lutterbaugh,
Graham Casey, Jean-Pierre Issa, Joseph Willis, James K. V. Willson, Christoph Plass, and Sanford Markowitz. SLC5A8:
A novel sodium transporter, is a tumor suppressor gene silenced by methylation in human colonic aberrant crypt foci and
colon cancers. PNAS, USA 100: 8412-8417, 2003. [128 citations]
33. Coady MJ, Chang M-H, Charron FM, Plata C, Wallendorff B, Sah JF, Markowitz SD, Romero MF, and Lapointe J-Y.
The human tumor suppressor gene SLC5A8 expresses a Na/monocarboxylate cotransporter. J Physiol 557: 719-731, 2004
(DOI: 10.1113/jphysiol.2004.063859). [73 citations]
34. Dinour D, M-H Chang, J-I Satoh, BL Smith, A Knecht, I Serban, EJ Holtzman, MF Romero. A Novel Missense Mutation
in the Sodium Bicarbonate Cotransporter (NBCe1/SLC4A4) reveals ion transport defects. J Biol Chem 279: 52238-46,
2004. [73 citations]
35. Choe K, Kato A, Hirose S, Plata C, Sinđić A, Romero MF, Claiborne J, and Evans D. NHE3 in an ancestral vertebrate:
primary sequence, distribution, localization, and function in gills. Am J Physiol: Regulatory, Integrative and Comparative
Physiology 289: R1520-1534, 2005. [38 citations]
36. Mackenzie B, Ujwal ML, Chang M-H, Romero MF, and Hediger MA. Divalent metal-ion transporter DMT1 mediates
both H+-coupled Fe
2+ transport and uncoupled fluxes. Pflügers Arch 451: 544 – 558, 2005. [45 citations]
37. Romero MF, Chang M-H, Plata C, Zandi-Nejad K, Broumand V, Sussman CR, and Mount DB. "Physiology of
electrogenic SLC26 paralogs" In - Epithelial Anion Transport in Health and Disease: the role of the SLC26 transporters
family. Novartis Foundation Symposium 273: 126-147, 2006. [14 citations]
38. Demirci FYK, Chang M-H, Mah TS, Romero MF, and Gorin MB. Proximal renal tubular acidosis and ocular pathology:
L522P, a novel missense mutation in the gene (SLC4A4) for Sodium Bicarbonate Cotransporter protein (NBCe1).
Molecular Vision 12:324-330, 2006. [9 citations]
39. Günzel DM, Kehres DG, Romero MF, and Maguire ME. The MgtC virulence factor of Salmonella enterica serovar
Typhimurium activates Na+, K
+-ATPase. J Bacteriology 188:5586-5594, 2006. [12 citations]
40. Huang C, Sindic A, Chan KW, Hill CE, Hujer KM, Sassen M, Wu Z, Kurachi Y, Nielsen S, Romero MF, and Miller RT.
Interaction of the Ca2+
sensing receptor with the inwardly-rectifying potassium channels inhibits channel function. Am. J.
Physiol 292: F1073-81, 2007 (doi:10.1152/ajprenal.00269.2006). [10 citations]
41. Plata C, Sussman CR, Liang JO, Mount DB, Josephs ZM, Chang M-H, and Romero MF. A novel electroneutral sodium
monocarboxylate transport (SMCTn) is present in zebrafish: A comparison to the electrogenic SMCT (SMCTe/Slc5a8). J
Biol Chem 282 (16): 11996-12009, 2007. [2 citation]
42. Kurita Y, Nakada T, Kato A, Mistry AC, Chang M-H, Romero MF, Hirose S. Identification of intestinal bicarbonate
transporters involved in formation of carbonate precipitates to stimulate water absorption in marine teleost fish. Am J
Physiol - Comp & Reg Physiol 284: R1402-1412, 2008. [27 citations]
43. Chang M-H, DiPiero JM, Sönnichsen FD, Romero MF. Entry to "HCO3 tunnel" revealed by SLC4A4 human mutation
and structural model. J Biol Chem 283: 18402-18410, 2008. [10 citations]
44. Chang M-H*, Plata C*, Zandi-Nejad K, Sinđić A, Sussman CR, Raghuram V, Mount DB, and Romero MF. Slc26A9 -
anion exchanger, channel and Na+
transporter. J Membr Biol 228: 125-40,2009 [11 citations] PMID- 19365592
45. Unal ES, Zhao R, Chang M-H, Fiser A, Romero MF, and Goldman ID. The Impact of Histidine Residues on the
Structure/Function of the Human Proton-Coupled Folate Transporter (HsPCFT), SLC46A1. J Biol Chem 284: 17846-
17857, 2009 [15 citations]
46. Sindic A, Chen AP, Huang C, Romero MF, and Miller RT. MUPP1 complexes renal K+
channels to alter surface
expression and transport. Am J Physiol Renal 297: F36-45, 2009.
MF Romero Curriculum vitae - September 2, 2011 14
47. Sussman CR, J Zhao, C Plata, JLC Daly, N Angle, J DiPiero, IA Drummond, JO Liang, WF Boron, MF Romero, MH
Chang. Cloning, localization and functional expression of the electrogenic Na+
bicarbonate cotransporter (NBCe1) from
zebrafish. Am J Physiol - Cell Physiol 297: C865-75, 2009. [5 citation] PMID 19625604.
48. Chang M-H, Plata C, Sinđić A, Ranatunga WK, Chen AP, Zandi-Nejad K, Chan KW, Thompson J, Mount DB, Romero
MF. Slc26a9 is inhibited by the R-region of CFTR via the STAS domain. J Biol. Chem. 284: 28306-28318, 2009. [8
citations] PMID- 19643730
49. Kato A, Kurita Y, Nakada T, Ogoshi M, Nakazato T, Doi H, Chang M-H, Hirose S, and Romero MF. Identification of
renal transporters involved in sulfate excretion in marine teleost fish. Am J Physiol Comp & Reg Physiol: 297: R1647-
1659, 2009 [4 citation] PMID 19812358
50. Mahadeo K, Bove N, Unal E, Teo J, Zhao R, Chang M-H, Fulterer A, Romero MF, and Goldman ID. Properties of the
Arg376 residue of the proton-coupled folate transporter (PCFT-SLC46A1) and a glutamine mutant causing hereditary
folate malabsorption. Am J Physiol - Cell Physiol doi:10.1152/ajpcell.00113.2010, 2010. PMID 20686069
51. Desmoulin SK, Wang Y, Wu J, Stout M, Hou Z, Fulterer A, Chang M-H, Romero MF, Cherian C, Gangjee A, and
Matherly LH. Targeting the proton-coupled folate transporter for selective delivery of 6-substituted pyrrolo[2,3-d]-
pyrimidine antifolate inhibitors of de novo purine biosynthesis in the chemotherapy of solid tumors. Molecular
Pharmacology doi:10.1124/mol.1110.065896, 2010. PMID 20686069
52. Romero MF. SNPs of metabolism, not stones. Am J Physiol Renal Physiol 299: F702-703, 2010, 2010.PMID 20685824
53. Bellemer A, T Hirata, MF Romero, and MR Koelle. Two types of chloride-extruding transporters are required for GABAA
receptor-mediated inhibition in C. elegans. EMBO J 30; 1852 - 1863, 2011. PMID 21427702
54. Roy-Chowdhury U, Bahler CK, Hann CR, Chang M-H, Resch ZT, Romero MF, and Fautsch MP. Activation of
Adenosine Triphosphate-Sensitive Potassium (KATP) Channels Decreases Intraocular Pressure in the Anterior Chamber
of the Eye. Invest Ophthalmol Vis Sci, 2011; DOI: 10.1167/iovs.11-7523 (in press)
55. Islam Z, Kato A, Romero MF, Hirose S. Identification and Apical Membrane Localization of an Electrogenic Na+/Ca
2+
Exchanger NCX2a Involved in Renal Ca2+
Excretion by Seawater Fish. Am J Physiol Regu, 2011 (in press)
doi:10.1152/ajpregu.00165.2011
(Manuscripts submitted / in revision)
1. Hirata T, Cabrero P, Berholtz DJ, Thompson JR, Dow JAT, and Romero MF. In vivo Drosophila model of calcium
oxalate nephrolithiasis. Science (in review), 2011.
2. Hinson SR, Romero MF, Popescu BF, Lucchinetti CF, Fryer JP, Wolburg H, Fallier-Becker P, Noell S, and Lennon VA.
Diverse Pathophysiological Outcomes of IgG Binding to Aquaporin-4 in Living Cell Membranes. PNAS, USA, 2011 (in
revision)
3. Chen A-P, MH Chang and Romero MF. A regulatory loop within STAS domain controls Cl- transport in human
SLC26A9. J Biol Chem, 2011 (in revision)
4. Plata C, M-H Chang, GT Babcock and MF Romero. Cloning and function of a Xenopus Sodium Monocarboxylate
Cotransporter. Am. J. Physiol Cell Physiol, in revision
5. Kimura Y, Kato A, Kurita Y, Chang M-H, Kasai K, Fujiwara T, Hirata T, Doi H, Romero MF, and Hirose S. Borate
excretion by seawater fish uses a renal Slc4a11-borate uniporter. Embo J (in revision), 2011
6. Umezawa T, Kato A, Ogoshi M, Ookata K, Doi H, Romero MF, and Hirose S. O2-filled swimbladder employs
monocarboxylate transporters for the generation of O2 by lactate-induced Root effect hemoglobin. Plos ONE, 2011.
7. Michelle Visentin, Min-Hwang Chang, Michael F. Romero, I. David Goldman. Substrate-specific folate transport
mediated by OATP2B1 at acidic pH. Molec. Pharmacol., 2011 (in revision)
8. Wang L, Desmoulin SK, Cherian C, Polin L, White K, Kushner J, Fulterer A, Chang M-H, Mitchell S, Romero MF, Hou
Z, Matherly LH, and Gangjee A. Synthesis, biological and antitumor activity of a highly potent 6-substituted pyrrolo[2,3-
d]pyrimidine thienoyl antifolate inhibitor with transport selectivity for the proton-coupled folate transporter and folate
receptor over the reduced folate carrier that inhibits β-glycinamide ribonucleotide formyltransferase. J Medicinal
Chemistry, 2011 (submitted).
9. Chang M-H, Plata C, Kurita Y, Kato A, Hirose S, and Romero MF. Functional characterization & physiology of NBCe1
from a euryhaline teleost. Am J Physiol Cell Physiol (in revision), 2011.
10. Chen AP, Chang M-H, and Romero MF. Functional analysis of non-synonymous single nucleotide polymorphisms in
human SLC26A9. Human Mutation: submitted, 8/17/2011, 2011
MF Romero Curriculum vitae - September 2, 2011 15
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(Manuscripts in preparation)
(Invited Manuscripts & Book Chapters)
1. Liedtke, CM, M Romero, and U Hopfer. 1988. Adrenergic control of the Na, K, 2Cl cotransporter in airway epithelial
cells. in Cellular and Molecular Basis of Cystic Fibrosis, G. Mastella and P.M. Quinton (eds.). San Francisco Press, Inc,
San Francisco, CA, pp. 307-313.
2. Romero, MF, Z Madhun, U Hopfer and JG Douglas. 1990. An Epoxygenase metabolite of arachidonic acid, 5,6-epoxy-
eicosatrienoic acid mediates angiotensin-induced natriuresis in proximal tubular epithelium. in Adv. in Prostaglandin,
Thromboxane, and Leukotriene Research: 7th International Congress on Prostaglandins and Related Compounds, Vol. 21
A (eds. Samuelsson, Paoletti and Ramwell). Raven Press, New York, New York, pp. 205-208. [40 citations]
3. Boron, WF, MA Hediger, EL Boulpaep, and MF Romero. The renal electrogenic Na/HCO3 cotransporter. J Exp. Biol.
200: 263-268, 1997. [38 citations]
4. Boron, WF, P Fong, MA Hediger, EL Boulpaep, MF Romero. The electrogenic Na/HCO3 cotransporter. Weiner
Klinische Wochenschrift, 109: 445-456, 1997. [10 citations]
5. Romero, MF, Y Kanai, H Gunshin and MA Hediger. Expression cloning using Xenopus laevis oocytes.
Meth Enz., 296: 17-52, 1998. [59 citations]
6. Romero, MF and WF Boron. Electrogenic Na/HCO3 cotransporters: Expression cloning and physiology.
Ann. Rev. Physiol. 61: 699-723, 1999. [138 citations]
7. Romero, M. F., C. M. Sciortino, E. Roussa, and F. Thévenod. Na+
/HCO–
cotransporters in various species and organs.
In: 3rd Annual Borelli Conference: Acid-base balance, edited by N. DeSanto. Napoli, Italy:, 1999.
8. Romero, MF. The electrogenic Na+/HCO
– cotransporter, NBC. Journal of the Pancreas (online) 2: 182-191, 2001. [13
citations]
9. Satoh, J-I, and MF Romero. Mg2+
transport in the kidney. Biometals 15: 285-295, 2002. [7 citations]
10. MA Hediger, DM Mount, A Rolfs, MF Romero. The Molecular Basis of Solute Transport. In “The Kidney.” Ed B.M.
Brenner, 7th
edition, 2004, pp.261-308.
11. Romero, MF. Defects of the electrogenic Na+/HCO
– cotransporter, NBC1/SLC4a4. In: Membrane transporter diseases,
edited by Bröer S and Wagner C. New York: Kluwer Academic/Plenum Publishers, 2004, p. 65-79.
12. Hediger MA, Romero MF, Peng J-B, Rolfs A, Takanaga H, and Bruford E. The ABC's of Solute Carriers: Physiological,
pathological and therapeutic implications of human membrane transport proteins: Introduction. Pflügers Arch 447: 465-
468, 2004. [266 citations]
13. Mount DB and Romero MF. The SLC26 gene family of multifunctional anion exchangers. Pflügers Arch 447: 710-721,
2004. [249 citations]
14. Romero, MF, CM Fulton, & Boron, WF. The SLC4 gene family of HCO -
2004. [215 citations]
transporters. Pflügers Arch, 447:495-509,
15. Romero MF. In the beginning, there was the cell: cellular homeostasis. Adv Physiol Ed 28: 135-8, 2004.
16. Romero MF. Molecular pathophysiology of SLC4 bicarbonate transporters. Curr Opin Nephrol Hypertens 14: 495-501,
2005. [31 citations]
17. Mount DB, Kwon CY, Plata C, Romero MF, and Zandi-Nejad K. SLC5 Sodium-Anion Cotransporters and Renal Urate
Transport. In: Renal Stone Disease: Proceedings of the First International Urolithiasis Research Symposium. A.P. Evan,
J.E. Lingeman, and J.C. Williams, eds. American Institute of Physics, Melville, NY, 2007.
18. Sinđić A, Chang MH, Mount DB, Romero MF. Renal physiology of SLC26 anion exchangers. Curr Opin Nephrol
Hypertens 16: 484-490, 2007. [16 citations]
19. Romero MF, Chang M-H and Mount DB. From cloning to structure, function, and regulation of chloride-dependent and
chloride independent bicarbonate transporters. In: Physiology and Pathology of Chloride Transporters and Channels in
the Nervous System: From molecules to diseases, F. Javier Alvarez-Leefmans M, Eric Delpire (ed): Elsevier Academic
Press, 2009, p. 43-80.
20. Dow JAT, Romero MF. Drosophila provides rapid modelling of renal development, function and disease. Am J Physiol
Renal Physiol: 299: F1237-1244, 2010. (doi:10.1152/ajprenal.00521.2010)
21. Kato A and Romero MF. Regulation of Electroneutral NaCl Absorption by the Small Intestine. Annual Review of Physiology vol 73: 261-81, 2011. [2 citations] PMID: 21054167
22. Sindic A, Sussman CR, Romero MF. Primers on Molecular Pathways - Bicarbonate transport by the pancreas. Pancreatology 10:660-4, 2010 (doi: 10.1159/000323435)
(Invited Reviews in preparation)
1. Romero MF and M-H Chang. CSH Protocols. “Electrophysiology using Xenopus oocytes ” 2011.
2. Romero MF, Chang M-H, Plata C, Sinđić A, Sussman CR, Chen AP, Ranatunga WK, Hirata T, Kato A, Hirose S, Mount
DB. Bicarbonate and Na-bicarbonate transporters – role of evolutionary nitches. J Exp. Biol, 2011.
MF Romero Curriculum vitae - September 2, 2011 16
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(Abstracts)
1. JW Jacobberger, U Hopfer, CM Liedtke, MF Romero, and TL Sladek. Characterization of cystic fibrosis airway
epithelial cells immortalized by transduction of the SV40 large T antigen gene. Society for Analytical Cytology.
Breckenridge, CO. , 1988 abstract.
2. JW Jacobberger, U Hopfer, CM Liedtke, MF Romero, and TL Sladek. Immortalization of airway epithelial cells by
transduction of wild-type and mutant SV40 large T antigen genes. Pediatric Pulmonology 126A. Presented at 1989
North America Cystic Fibrosis Conference, Tarpon Springs, Fla., Oct. 11-14, 1989.
3. MF Romero, JG Douglas, and U Hopfer. 1989. Angiotensin II increases Na+ absorption in early S1 primary cultures. J.
Cell Biol. 109(4):210 (Abstract).
4. MF Romero, JG Douglas, and U Hopfer. 1990. P450 monooxygenase metabolite of arachidonic acid inhibits Na+
absorption in early S1 primary cultures. Kidney Int. 37: 350 (Abstract).[3 citations]
5. S Andreatta-van Leyen, MF Romero, MC Khosla, CM Ferrario, and JG Douglas. 1990. Angiotensin 1-7 (Ang 1-7)
stimulates phospholipase A2 (PLA2) and modulates Na+ Transport in renal tubular epithelium (RTE). Presented at
Council for High Blood Pressure Research 44th Annual Fall Conference (sponsored by Am. Heart Assoc.), Baltimore,
MD, Sept. 12-15, 1990. Hypertension 16(3):328, 1990. [5 citations]
6. MF Romero, JG Douglas, RL Eckert, U Hopfer, and JW Jacobberger. Development and characterization of new proximal
tubular epithelial cell lines. J. Cell Biol. 115: 359, 1991.
7. GI Gorodeski, MF Romero, and WH Utian. Human ectocervical epithelial cultures grown on porous filter: electrical and
morphological characteristics. J. Cell Biol. 115: 358, 1991.
8. MF Romero, JW Jacobberger, and U Hopfer. Angiotensin II and bradykinin stimulated electrogenic ion transport in the
proximal tubule epithelial cell line vEPTa-PL. Biophys. J. 61: A536, 1992.
9. GI Gorodeski, MF Romero, RL Eckert, U Hopfer. Regulation of paracellular transport in human uterine cervical
epithelium. Mol. Biol. Cell 3: 219, 1993. [3 citations]
10. EM Hogan, MF Romero, BA Davis, WF Boron. Shrinkage-induced activation of Na-H exchange in Xenopus oocytes.
FASEB J. 7: A442, 1993.[1 citation]
11. U Hopfer, MF Romero, Z Madhun, JG Douglas. Angiotensin II responses of proximal tubule cells in primary culture and
in a newly generated proximal tubular Cell line. XIIth International Congress of Nephrology. Jerusalem, Israel, June,
1993.
12. MF Romero, Y Kanai, MA Hediger, WF Boron. Acid-base transport is coupled to the electrogenic high-affinity
glutamate transporter, EAAC1. J. Am. Soc. Nephrol. 4:846, 1993.
13. MF Romero, S Nußberger, Y Kanai, MA Hediger, WF Boron. H+ Cotransport is coupled the electrogenic oligopeptide
transporter, PepT1.For presentation at FASEB in Anaheim, CA., FASEB J. 8: A561, 1994.
14. MF Romero, S Nußberger, MA Hediger, and WF Boron. H+ Cotransport Via the electrogenic oligopeptide transporter
PepT1 is bidirectional and Depends on external pH. J. Am. Soc. Nephrol. 5: A260, 1994.
15. MF Romero and WF Boron. Expression of kidney poly(A)-RNA in Xenopus oocytes speeds the CO2 -induced
intracellular acidification. FASEB J. 9: A260, 1995.
16. NL Nakhoul, MF Romero, BA Davis, and WF Boron. Expression of CHIP28 (Aquaporin 1) in Xenopus oocytes
accelerates the CO2 -induced decrease of intracellular pH (pHi). J. Am. Soc. Nephrol. 6: A312, 1995.
17. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Expression cloning of the renal electrogenic Na/HCO3
cotransporter (NBC-1) from Ambystoma tigrinium. FASEB J. 10: A89, 1996.
18. NL Nakhoul, MF Romero, A Waheed, BA Davis, R Mullins, G Hageman, WS Sly, and WF Boron. Processing and
functional expression of carbonic anhydrase isoforms in Xenopus laevis oocytes. FASEB J. 10: A88, 1996.
19. S Nussberger, A Steel, MF Romero, CAR Boyd, WF Boron, and MA Hediger. Proton coupling and charge movement of the
proton-dependent oligopeptide transporter PepT1. FASEB J. 10: A79, 1996.
20. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Expression Cloning of the Renal Electrogenic Na/HCO3
Cotransporter (NBC) from Ambystoma tigrinum. The Physiologist 39: 144, 1996
21. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Physiology of the Cloned Renal Electrogenic Na/HCO3
Cotransporter, NBC. J. Gen. Physiol. 108: 16-17a, 1996.
22. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Physiology of the cloned Ambystoma tigrinum Renal Electrogenic
Na/HCO3 Cotransporter (aNBC): I. Role of HCO3 J. Am. Soc. Nephrol. 7: 1259, 1996.
23. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Physiology of the cloned Ambystoma tigrinum Renal Electrogenic
Na/HCO3 Cotransporter (aNBC): II. Localization and Na+ Dependence. J. Am. Soc. Nephrol. 7: 1260, 1996.
24. MF Romero, MA Hediger, and WF Boron. Cloning and Functional Expression of the Rat Renal Electrogenic Na/HCO3
Cotransporter (rNBC). J. Am. Soc. Nephrol. 7: 1259, 1996.
25. II Grichtchenko, MF Romero, and WF Boron. Extracellular bicarbonate dependence of aNBC, the electrogenic Na/HCO3
cotransporter cloned from tiger salamander (Ambystoma tigrinum ). J. Am. Soc. Nephrol. 7: 1255, 1996.
MF Romero Curriculum vitae - September 2, 2011 17
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26. MF Romero, MA Hediger, P Fong, & WF Boron. Expression of the Rat Renal Electrogenic Na/HCO3 Cotransporter (rNBC)
Selected for oral presentation in “Latest Advances: Theme I” at the XIV th International Society of Nephrology, May 25, 1997,
Sydney, Australia.
27. MF Romero, MA Hediger, P Fong, & WF Boron. Expression of the Rat Renal Electrogenic Na/HCO3 Cotransporter (rNBC)
FASEB J. 11: A25, 1997.
28. II Grichtchenko, MF Romero, and WF Boron. Extracellular bicarbonate dependence of the cloned rat electrogenic Na/HCO3
cotransporter (rNBC). For presentation at the XXXIII th International Congress of Physiological Sciences, St. Petersburg,
Russia, 1997.
29. BM Schmitt, D Biemesderfer, EL Boulpaep, MF Romero, and WF Boron. Immunolocalization of the electrogenic Na/HCO3
cotransporter (NBC) in mammalian and amphibian kidney. J. Am. Soc. Nephrol. 8:10A , 1997.
30. II Grichtchenko, MF Romero, and WF Boron. Electrogenic Na/HCO3 cotransporters (NBC) from rat and salamander kidney
have similar external HCO–
dependencies. FASEB J. 12: A638, 1998. [1 citation]
31. MO Bevensee, BM Schmitt, MF Romero, and WF Boron. Cloning of a putative Na/HCO3 cotransporter (NBC) from rat
brain. FASEB J. 12: A1031, 1998. [1 citation]
32. BM Schmitt, D Biemesderfer, S Breton D Brown, R Caroppo, JP Geibel, M Ikuma, V Rajendran, EL Boulpaep, MF Romero,
and WF Boron. Immunolocalization of the electrogenic Na+/HCO
– cotransporter (NBC) in mammalian epithelia. Pflüger
Arch. 435: R59, 1998.
33. Rimmer, SJ, Romero, MF, Guggenheim, JG, Wigham, CG, Hodson, SA. Demonstration of a sodium-bicarbonate
cotransporter (NBC) mRNA in rabbit corneal endothelium. Exp. Eye Research 67: S8, 1998.
34. Romero, MF, Sussman, CR, Choi, I, Hediger, MA and Boron, WF. Cloning of an electrogenic Na/HCO3 cotransporter
(NBC) isoform from human kidney and pancreas. J. Am. Soc. Nephrol. 9: 11A, 1998.
35. Romero, MF, Davis, BA, Sussman, CR, Bray-Ward, P, Ward, D and Boron, WF. Identification of multiple genes for human
electrogenic Na/HCO3 cotransporters (NBC) on 4q. J. Am. Soc. Nephrol. 9: 11A, 1998.
36. Romero, MF and Boron, WF. Identification and expression of an electroneutral Na/HCO3 cotransporter from
Caenorhabditis elegans (ceNBC). J. Am. Soc. Nephrol. 9: 11A, 1998.
37. Müller-Berger, S, Heyer, M, Romero, MF , Boron, WF and Frömter, E. Stoichiometry of rat Na+-HCO3 cotransporter
(rkNBC) overexpressed in Xenopus laevis oocytes measured using giant patches. J. Am. Soc. Nephrol. 9: 9A, 1998.
38. Sciortino CM, & Romero MF. Na+
and voltage dependence of the rat kidney electrogenic Na/HCO3 cotransporter (rkNBC)
expressed in Xenopus oocytes. J. Am. Soc. Nephrol. 9: 12A, 1998.
39. M. Heyer, S. Müller-Berger, M.F. Romero, W.F. Boron and E. Frömter. Symmetry test of rat renal Na-HCO3 cotransporter
(rkNBC) overexpressed in Xenopus laevis oocytes. Pflüger Arch. 1999. Presented at the German Physiology Society, Dec,
1998, Bonn, Germany.
40. Thévenod, F. Roussa, E., Schmitt BM, & Romero MF. Molecular cloning, biochemical characterization and
immunolocalization of the rat pancreatic Na+
HCO–. FASEB J. 13: A73, 1999.
41. Choi I, Aalkjaer C, Romero MF, and Boron WF. Cloning and characterization of an electroneutral Na-driven bicarbonate
transporter from rat pulmonary arteries. FASEB J. 13: A400, 1999. [1 citation]
42. Mackenzie B, Ujwal ML, Rolfs A, Berger UV, Romero MF, and Hediger MA. Probing the molecular mechanisms of the H+-
coupled metal-ion transporter DCT1 and the regulation of its expression. FASEB J. 13: A537, 1999.
43. M Ganz, A. K. Dillon, C. M. Sciortino, and M. F. Romero. The Na+ driven Cl-HCO3 exchanger is Actually a Na+ Driven Anion Exchanger, NDAE1. J. Am. Soc. Nephrol. 10: 4A, 1999.
44. Romero, M. F., D. Henry, S. Nelson, E. P. Jane, P. J. Harte, and C. M. Sciortino. Cloning, Localization and Expression of a
Na+ Driven Anion Exchanger, NDAE1. J. Am. Soc. Nephrol. 10: 8A, 1999.
45. Sciortino, C. M., and M. F. Romero. Characterization of Leak Current, Substrates, and Inhibitors of the Na+-driven Anion
Exchanger (NDAE1). J. Am. Soc. Nephrol. 10: 9A, 1999.
46. Romero, M. F., A. K. Dillon, P. J. Harte, and C. M. Sciortino. The Drosophila ndae1 Gene Encodes a Functional Na+
Dependent Cl-HCO3 Exchanger. Presented at NIH Workshop-Advances in Membrane Transport, Bethesda, MD; Dec 6-7,
1999.
47. Sciortino, C. M., and M. F. Romero. Anion and cation transport properties of the Na+
driven anion exchanger (NDAE1)
expressed in Xenopus oocytes. FASEB J. 14: A356, 2000. [1 citation]
48. Romero, M. F., A. K. Dillon, P. J. Harte, and C. M. Sciortino. The Drosophila ndae1 Gene Encodes a Functional Na+
Dependent Cl-HCO3 Exchanger. FASEB J. 14: A356, 2000.
49. Romero, MF, F Thévenod, E Roussa, LD Shrode, & CM Sciortino. Physiology and Localization of Electrogenic Na/HCO3
Cotransporters (NBC). American Heart Association Research Symposium, May 6, 2000, Dallas, TX
50. Moffett, D. F., S.B. Moffett and M.F. Romero. Immunolocalization of a Na+-dependent anion exchanger (NDAE1) in
anterior stomach of mosquito larvae (Aedes aegypti): relevance to gut alkalinization. Society for Insect Cell Biology,
Chicago, Il., Jan. 2001, AM ZOOL 40: (6) 1134-1134 DEC 2000.
51. Sciortino, CM and MF Romero. Functional Characterization of a Na+/HCO
– Cotransporter Point Mutation that results in
Type II Renal Tubular Acidosis. FASEB J 15: (4) A502-A502 Part 1 MAR 7 2001. [2 citations]
MF Romero Curriculum vitae - September 2, 2011 18
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52. Sciortino, CM, LD Shrode, BR Fletcher, PJ Harte, and MF Romero. NDAE1, Na+–driven anion exchanger from Drosophila,
is a Plasma Membrane Protein Expressed in the Nervous System and Alimentary Tract FASEB J 15: (4) A503-A503, 2001.
[1 citation]
53. Romero, MF, and BL Smith. Mutations in the Na+/HCO
– cotransporter result in biophysical alterations causing renal tubular
acidosis and ocular pathologies. FASEB J 16:A52-3, 2002.
54. Xie, Qizhi2, Rick Welch
2, Adriana Mercado
1,2, Michael F. Romero
3, and David B. Mount
1,2. Cloning of the SLC26A6 anion
exchanger, functional comparison to SLC26A1. FASEB J 16: A807, 2002.
55. Xie, Qizhi2, Rick Welch
2, Luyan Song
,2, Michael F. Romero
3, and David B. Mount
1,2. Cloning of multiple novel members of
the SLC26 gene family of anion exchangers. FASEB J 16: A807, 2002.
56. Xie, Qizhi2, Michael F. Romero
3, David B. Mount
1,2. Multiple novel members of the SLC26 gene family of anion
exchangers. Days of Molecular Medicine 2002 conference, March 13-16, 2002 at The Salk Institute in La Jolla, California.
57. Chang M-H, DiPiero J, Sönnichsen FD, and Romero MF. Mutational analysis predicts structure and ion transport role for the
N-terminus of the electrogenic Na+ bicarbonate cotransporter, NBC1 (SLC4a4). FASEB J 17: A462, 2003.
**Young Investigator award for Cell & Molecular Physiol. Section APS, EB 2003
58. Josephs Z, Satoh J-i, Chang M-H, Mercado A, Zandi-Nejad K, Mount DB, and Romero MF. Cloning and expression of
Drosophila Slc4 and Slc26 homologs. FASEB J 17: A462, 2003.
**Young Investigator award for Comparative Physiol. Section APS, EB 2003
59. Mackenzie B, Ujwal ML, Chang M-H, Romero MF, and Hediger MA. Resolving the mechanisms of the iron transporter
DCT1 from kinetic properties and the impact of mutating two critical histidyl residues within transmembrane regions.
FASEB J 17: A911, 2003. [1 citation]
60. Mercado A, Zandi-Nejad K, Romero MF, and Mount DB. Comparative physiology of Xenopus laevis SLC26 exchangers.
FASEB J 17: A464, 2003.
61. Zandi-Nejad K, Enck A, Mercado A, Romero MF, and Mount DB. The three Xenopus laevis orthologs of mammalian
pendrin/SLC26A4 differ in oxalate transport and DIDS-sensitivity. J Am Soc Nephrol, 14: 542A, 2003.
62. Chang M-H, Satoh J-I, Brenda L. Smith, and Romero MF. The S427L mutation in human kidney NBC1 (SLC4A4) is a
transport defect. Faseb J 18: A1269, 2004.
63. Josephs ZM and Romero MF. Identification & localization of Slc4 & Slc26 anion transporters in Drosophila and zebrafish.
Faseb J 18: A317, 2004.
64. MF Romero, DB Mount*, J-I Satoh, BL Smith, J DiPiero, N Angle, Z Josephs, CR Sussman, C Plata, F Sönnichsen, M-H
Chang. Sodium/bicarbonate cotransporters and new bicarbonate transporters in the kidney. Clinical and Experimental
Nephrology, 2004.
65. Plata C, Josephs ZM, and Romero MF. Cloning, Physiology & Localization of Xenopus and zebrafish Sodium
Monocarboxylate Cotransporters. J Am Soc Nephrol 15: 77A, 2004.
66. Romero MF, Chang M-H, DiPiero JM, Josephs ZM, Sussman CR, Angle NM, and Plata C. Cloning, expression &
localization of a renal electrogenic Na+
bicarbonate cotransporter from zebrafish, zNBCe1. J Am Soc Nephrol 15: 75A,
2004.
67. Zandi-Nejad K, Plata C, Enck AH, Romero MF, Mercado A, and Mount DB. Slc5a8 functions as a sodium-dependent
pyrazinoate and nicotinate cotransporter; implications for renal urate transport. J Am Soc Nephrol 15: 89A, 2004.
68. Chang M-H, Plata C, and Romero MF. Acid-base transport by the tumor suppressor SLC5A8, an electrogenic
Na+/butyrate & Na+/lactate cotransporter. J Am Soc Nephrol 15: 75A, 2004.
69. Chang M-H, Plata C, Zandi-Nejad K, Mount DB, and Romero MF. Slc26A9 is a highly electrogenic and Na+
transporting
anion exchanger. FASEB J 19: A656, 2005.
70. Plata C, Chang M-H, and Romero MF. Cloning and functional characterization of a zebrafish, electrogenic, sodium
monocarboxylate cotransporter (Slc5A8 / zSMCTe). FASEB J 19: A671, 2005
71. Sussman CR, Chang M-H, Plata C, Angle NM, and Romero MF. Localization, function and gene structure of a zebrafish
electrogenic Na+
bicarbonate cotransporter, NBCe1. FASEB J 19: A646, 2005
72. Choe KP, Kato A, Hirose S, Plata C, Romero MF, Claiborne JB, and Evans DH. NHE3 in an ancestral vertebrate: function
in elasmobranch gills. FASEB J 19: A638, 2005.
73. Fukuda H, Hirata T, Nakamura N, Wakabayashi S, Romero MF, and Hirose S. Molecular characterization and
immunolocalization of a bicarbonate transporter highly and predominantly expressed in rat choroid plexus epithelial cells
(on CD-ROM). Faseb J 19: A#922.923, 2005
74. Gorin MB, Demirci FY, Chang M-H, Mah TS, and Romero MF. A novel missense mutation in the Sodium Bicarbonate
Cotransporter (NBCe1/SLC4A4) in a patient with proximal renal tubular acidosis and ocular pathology.
Am Soc Human Genetics, 2005
75. Sinđić A, Plata C, Sussman CR, Chang M-H, and Romero MF. Localization of Slc26a9 and role of the STAS domain.
FASEB J 20: A839, 2006.
76. Chang M-H, Babcock GT, Hirose S, and Romero MF. Functional characterization & physiology of NBCe1 from a marine
teleost. FASEB J 20: A1233, 2006.
MF Romero Curriculum vitae - September 2, 2011 19
77. Plata C, Sussman CR, Josephs ZM, Chang M-H, Liang JO, and Romero MF. Localization and Functional Characterization
of Two Na+/Monocarboxylate Transporters in zebrafish (zSMCT): electrogenic (zSMCTe/Slc5a8) and electroneutral
(zSMTn/Slc5a12). FASEB J 20: A1234, 2006.
78. Sussman CR, Babcock GT, Plata C, Liang JO, and Romero MF. Cloning and characterization of zebrafish epidermal
growth factor receptor family member, ErbB4. FASEB J 20: A1273, 2006.
79. Sindic A, Huang C, Che D, Ding Y, Romero MF, and Miller RT. Interaction of Kir4.2, a Distal Nephron K+ Channel with
MUPP1, a PDZ Domain-Containing Protein, Results in Increased Cell Surface Expression and Activity of Kir4.2. J Am
Soc Nephrol 2006.
80. Chang M-H, Babcock GT, and Romero MF. Evidence of functional NBCe1 (SLC4A4) dimer assembly. FASEB J 21,
2007.
81. Sindic A, Mount DB, Plata C, Sussman CR, and Romero MF. The rat Slc26a9 anion transporter is located in many
epithelial tissues. Pediatric Pulmonology, 2007
82. Kato A, Chang M-H, Kurita Y, Nakada T, Hirose S, and Romero MF. Functional Characterization of Pufferfish Slc26a6A
and Slc26a6B. Faseb J 22, 2008
83. Plata C, Juárez ME, Vázquez N, Gamba G, and Romero MF. Valproate and Nonsteroidal Anti-Inflammatory Drugs
Reduces the activity of electroneutral (SMTn/Slc5a12) and electrogenic (SMCTe/Slc5a8) Na+/Monocarboxylate
Transporters (SMCT). Faseb J 22: 1202.12, 2008.
84. Plata C, Vázquez N, Romero MF, and Gamba G. Opposite effects of WNK3 and WNK4 upon the Slc26a9 anion
transporter/channel. Faseb J 22: 1202.9, 2008
85. Kurita Y, Nakada T, Kato A, Doi H, Mistry AC, Chang M-H, Romero MF, and Hirose S. Identification of Intestinal
Bicarbonate Transporters Involved in Formation of Carbonate Precipitates to Stimulate Water absorption in Marine
Teleost Fish. Faseb J 22, 2008.
86. Liang J, Manieri A, Kramek H, Kuranovic D, Robinson J, Romero MF. Zebrafish in the Classroom: Building an
Educational Website. Zebrafish meeting, Madison, WI, 2008
87. Chen A-P, Chang M-H, Sindic A, Mount DB, Romero MF. Single nucleotide polymorphisms (SNPs) of human
SLC26A9. FASEB J 23: 796.24, 2009
88. Chang M-H, Holmes HL, Ranatunga WK, Chen A-P, Romero MF. NBCe1 (SLC4A4) functional dimer assembly. Faseb J
23, 2009
89. Lopez A, Vazquez N, Torres N, Zandi-Nejad K, Mount DB, Gamba G, Tovar A, Romero MF, Plata C. SGK1 and Insulin
reduce the activity of mammalian electrogenic Na+/Monocarboxylate Transporters (SMCTe/Slc5a8). Faseb J 23: 797.11,
2009
90. Romero MF, Sindic A, Sussman CR, Chang M-H, Plata C, Chen A-P, Mount DB, Bahler CK, and Fautsch MP. Slc4 and
Slc26 bicarbonate transporter function and localization in the eye. Invest Ophthalmol Vis Sci 50: E-Abstract 3628, 2009
91. Hirata T, Kato A, Cabrero P, Chang M-H, Dow JAT, and Romero MF. Drosophila Slc26a5 functions as a Cl-/Oxalate
exchanger similar to mammalian Slc26a6. J Am Soc Nephrol, 2009.
92. Dow JAT, Cabrero P, Hirata T, Chang M-H, and Romero MF. A simple model for nephrolithiasis: Drosophila Prestin
(Slc26a5) functions as a Cl-/Oxalate exchanger in the gut with kinetics similar to mammalian Slc26a6. J Physiol, 2009.
93. Brin LR, Hirata T, Scileppi EM, Holmes HL, Linser PJ, and Romero MF. Characterization of Anopheles gambiae Slc26a5
and potential role in malaria. Faseb J: 24:1002.25, 2010
94. Hirata T, Kato A, Chang M-H, Hirose S, and Romero MF. Pufferfish Slc26a5 (prestin) exchanges Cl- for oxalate, sulfate,
and bicarbonate. Faseb J: 24:1002.24, 2010.
95. Li Q, Babilonia E, Chang M-H, Kwon C, Mercado A, Mandal A, Romero MF, and Mount DB. A role for C-terminal
cysteines in dimerization of the Slc26a6 anion transporter. Faseb J: 609.13, 2010.
96. Chen A-P, Romero MF. Channel inhibitory region within the STAS domain of human SLC26A9. Faseb J: 1002.7, 2010
97. Hirata T, Cabrero P, Dow JAT, and Romero MF. Drosophila Prestin provides an in vivo model for oxalate kidney stone
formation. J Am Soc Nephrol 21: in press, 2010
98. Chang M-H and Romero MF. NBCe1 (SLC4A4) functions as a dimer and requires both the N- and C-termini. FASEB J
25, 2011
99. Roy-Chowdhury U, Bahler CK, Hann CR, Chang M-H, Romero MF, and Fautsch MP. Additive Effect of Latanoprost-
Free Acid and Diazoxide, an ATP-Sensitive Potassium (KATP) Channel Opener, on Outflow Facility Increase in Human
Anterior Segment Culture Invest Ophthalmol Vis Sci, 2011
Invited Talks:
1. MF Romero, JG Douglas, U Hopfer, and JW Jacobberger. Characterization of a proximal tubular epithelial cell line.
Workshop on the Development of Cell Lines for Hypertension Research, National Heart, Lung, and Blood Institute,
Washington, D.C., February 19-20, 1991.
2. U Hopfer, MF Romero, Z Madhun, JG Douglas. Angiotensin II responses of proximal tubule cells in primary culture and
in a newly generated proximal tubular cell line. XIIth International Congress of Nephrology. Jerusalem, Israel, June,
1993.
MF Romero Curriculum vitae - September 2, 2011 20
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3. March 10, 1995: “How do Xenopus oocytes move CO2 and H2O ?” Dept. Biology, Thomas More College, Crestview
Hills, KY. Invited by Dr. John Hageman.
1996
4. April 22, 1996: “Expression cloning of the renal electrogenic Na/HCO3 cotransporter, NBC,” Dept Cellular & Molecular
Physiology, Yale University, New Haven, CT. Invited by Dr. Brent Thompson.
5. May 7, 1996: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Div
Nephrology, Dept Medicine, Einstein, Bronx, NY. Invited byDr. Victor L. Schuster.
6. May 22, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept
Physiology, Univ. Maryland, Baltimore, MD. Invited by Dr. Paul Welling.
7. May 28, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept
Physiology, Cornell University, NY, NY. Invited by Dr. Henry Sackin.
8. May 30, 1996: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Renal
Division Brigham & Women’s Hospital, Boston, MA. Invited by Dr. Steve Gullins.
9. June 27, 1996: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Dept
Molecular Medicine, Beth Israel Hospital, Boston, MA. Invited by Dr. Seth Alper.
10. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. The electrogenic sodium bicarbonate cotransporter: expression
cloning and physiology. Am Physiological Society Conference I: pHysiology of acid-base regulation. Snowmass, CO,
July 12-15, 1996.
11. Sept. 18, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept
Biochemistry and Molecular Biology, St. Louis Univ. St Louis, MO. Invited by Dr. William S. Sly
12. Nov. 11, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Division of
General Medical Sciences, Univ California at Davis, Davis, CA. Invited by Dr. Paul Fitzgerald.
13. Dec. 2, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept
Physiology, University of Arkansas at Little Rock, Little Rock, AR. Invited by Dr. Michael Jennings.
14. Dec. 19, 1996: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Dept
Physiology and Biophysics, Univ. Alabama at Birmingham, Birmingham, AL. Invited by Dr. Dale Benos.
1997
15. MF Romero, MA Hediger, P Fong, & WF Boron. Expression of the Rat Renal Electrogenic Na/HCO3 Cotransporter
(rNBC). “Latest Advances: Theme I” at the XIV th International Society of Nephrology, Sydney, Australia, May 25-29,
1997.
16. Jan 31, 1997: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Dept
Physiology and Biophysics, Finch University, The Chicago Medical School, North Chicago, Il. Invited by Dr. Robert
Rokowski.
17. June 16, 1997: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Dept
Physiology and Biophysics, Case Western Reserve University, Cleveland, OH. Invited by Dr. Richard Eckert.
1998
18. Feb 13, 1998: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” MRC Group
in Membrane Biology, Univ. Toronto and Cell Biology Division , Hospital for Sick Children, Toronto, Ontario, Canada.
Invited by Dr. Reinhart A.F. Reithmeier
19. March 9, 1998: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning, physiology, and genomic
frontiers” Dept. Genetics and Center for Human Genetics, Case Western Reserve University, Cleveland, OH. Invited by
Dr. Gregg Mattera.
20. May 19, 1998: “Electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept. Pharmacology,
Case Western Reserve University, Cleveland, OH. Invited by Dr. John Nelson.
21. MF Romero and CM Sciortino. Cation and voltage dependence of a rat, renal electrogenic Na/HCO3 cotransporter
(rkNBC) expressed in Xenopus oocytes. Membrane Transport Proteins-Gordon Conference, Tilton, NH, 7/19-24, 1998.
1999
22. Feb. 1, 1999: "Cloning and characterization of electrogenic Na+/HCO
– cotransporters (NBCs)." Physiologisches Instiut
der Universität Zürich, Zürich, SWITZERLAND. Invited by Prof. Dr. Heini Murer
23. Feb. 2, 1999: "Cloning and characterization of electrogenic Na+/HCO
– cotransporters (NBCs)." Abteilung fuer
Allgemeine Zoologie, FB Biologie, Universitaet Kaiserslautern, Kaiserslautern, GERMANY. Invited by Prof. Dr.
Joachim W. Deitmer.
24. Feb. 4, 1999: "Cloning and characterization of electrogenic Na+/HCO
– cotransporters (NBCs)." Zentrum der Physiologie,
Klinikum der J.W. Goethe-Universitaet, Frankfurt/Main, GERMANY. Invited by Prof. Dr. Eberhard Frömter.
25. Feb. 22, 1999: "Cloning and characterization of electrogenic Na+/HCO
– cotransporters (NBCs)." Division of Pediatric
Pulmonology- Cystic Fibrosis Center-Rambow Babies & Children's Hospital. Invited by Dr. Mitch Drumm.
26. May 25, 1999: "Electrogenic Sodium Bicarbonate Cotransporters (NBC's)." Rammelcamp Center for Education and
Research at MetroHealth Medical Center.
MF Romero Curriculum vitae - September 2, 2011 21
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27. MF Romero. Cloning and characterization of electrogenic Na+/HCO
– cotransporters (NBCs). Foster Club -Dept.
Physiology, Cambridge University, Cambridge, England, UK; Jan. 27, 1999.—Invited by Prof. Roger Thomas
28. MF Romero. Cloning and characterization of electrogenic Na+/HCO
– cotransporters (NBCs)--an ion transporter of the eye.
Department of Optometry and Vision Sciences, University of Wales Cardiff, Wales, UK; Jan. 29, 1999—Invited by Drs.
Hodgson, Wiggham, and Rimmer.
29. MF Romero. Electrogenic Na+- HCO
– cotransporters (NBCs): acid loader or acid extruder?. Invited Symposium Speaker:
'Acid/Base Transporters in Epithelial Cells', Physiology Society Meeting Manchester, England, UK, March 29-31, 1999.
30. Romero, MF. Na/HCO3 cotransporters in various tissues. Borelli Acd-Base Conference, Anacapri, Italy, Oct. 1-3, 1999.
31. Romero, M. F., D. Henry, S. Nelson, E. P. Jane, P. J. Harte, and C. M. Sciortino. Cloning, Localization and Expression of
a Na+ Driven Anion Exchanger, NDAE1. 1999 American Society of Nephrology " Free communication" in Acid-Base
Transport, November 6, 1999.
32. Romero, MF. Transport pathways in prostate epithelium. In Nonrenal epithelia of the urogenital tract: lessons from the
kidney applied to physiology and pathophysiology. 1999 American Society of Nephrology Basic Science Symposia,
November 8, 1999.
2000
33. Feb 11, 2000: " Cloning and Expression of a Na+
Driven Anion Exchanger, NDAE1." NIH, NHLBI: Laboratory of
Kidney and Electrolyte Metabolism. Invited by Mark A. Knepper.
34. Feb 24, 2000: "Cloning and expression bicarbonate transporters." Dept. Biology, Kenyon College, Kenyon, OH. Invited
by Karen A. Hicks
35. July 28, 2000: "Cloning and Expression of a Na+
Driven Anion Exchanger, NDAE1." Membrane Transport Group, Depts.
Biochemistry and Medicine, University of Toronto, Toronto, Canada. Invited by Dr. Reinhart A.F. Reithmeier.
36. Oct. 13, 2000: Co-chair of Free Communication Session "Transport of proton and base" at the Am. Soc. Nephrology
annual meeting, Toronto, Canada.
2001
37. Jan. 12, 2001: "Cloning and expression bicarbonate transporters." Dept. Physiology and the CIHR Group in Molecular
Biology of membrane proteins, University of Alberta, Edmonton, Alberta, Canada.
38. March 16, 2001: " Na+
coupled bicarbonate transporters - views across Phyla" Invited Symposium Speaker: 'Acid/Base
Transporters in Epithelial Cells', Physiology Society Meeting, Oxford, England, UK.
39. April 2001: "Na+ coupled bicarbonate transporters: amphibians, mammals, and flies --- oh my" Experimental Biology
2001 Symposium - Molecular mechanisms of HCO– transport. Chair and organizier for Epithelial transport and Renal
Sections of Am. Physiol. Soc., Orlando, FL
40. April 27, 2001: "The electrogenic Na+/HCO
– cotransporter, NBC." Renal Research Conference, Renal Division,
Vanderbilt University School of Medicine, Nashville, TN. Invited by David Mount.
41. April 27, 2001: "Na+
coupled HCO–
transport- through the eyes and guts of flies." Membrane Biology Group, Vanderbilt
University School of Medicine, Nashville, TN. Invited by Kevin Strange, Director Anaesthesia Research.
42. June 5-6, 2001: "Na+
coupled bicarbonate transporters: amphibians, mammals, and flies --- oh my" 19th Annual
Symposium on "Ion transport in kidney and liver: a molecular view," GRTM (Membrane Transport Research Group) ,
Montreal, Canada.
43. Aug 26-31, 2001: "Na+
coupled bicarbonate transporters." Symposium “Cell Bicarbonate Transport " 34th
IUPS
Congress 2001. Christchurch, New Zealand.. Invited by Drs. D. C. Macknight and S.L. Alper.
2002
44. March 20, 2002: “Bicarbonate transporters.” Satelite Symposium “Bicarbonate Transport" at 45th Annual Meeting of
the Canadian Society of Biochemistry, Molecular and Cellular Biology (CSBMCB) and Winternational Symposium on
Membrane Proteins in Health and Disease. Invited by Joe Casey and Reinhart A. F. Reithmeier.
45. April 16, 2002: “The gasly details of bicarbonate transporters- a tale of 2 families.” Pharmacology, CWRU, Cleveland,
OH. Invited by Amy Wilson-Delfosse.
46. April 19, 2002: “Bicarbonate transporters-something old and something new. ” Dept. Physiology & Biophysics, Tulane
University, New Orleans, LA. Invited by Dr. Mouhamed Awayda.
47. April 20-24, 2002: “Recent insights into renal acid-base transporters.” Symposium “Renal Acid-Base Transporters"
Experimental Biology 2002.
48. July 11, 2002: “Na+
coupled bicarbonate transporters”Annual Review of Nephrology – Mexico City, Mexico. Invited by
Gerardo Gamba MD PhD.
49. July 11, 2002: “The gasly details of bicarbonate transporters- a tale of 2 families.”Annual Review of Nephrology –
Mexico City, Mexico. Invited by Gerardo Gamba MD PhD.
50. October 18, 2002: “Bicarbonate transporters- new and old.” NIH, NHLBI: Laboratory of Kidney and Electrolyte
Metabolism. Invited by Mark A. Knepper, Chief.
51. December 10, 2002: “Flavors of bicarbonate transporters- old and new.” Dept. Pathology, Emory University. Invited by
Dr. Didier Merlin.
MF Romero Curriculum vitae - September 2, 2011 22
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52. December 10, 2002: “Transport modes of the electrogenic Na+
bicarbonate cotransporter, NBC1.” Dept. Medicine -
Nephrology, Emory University. Invited by Dr. Jeffery Sands, Nephrology Division head.
2003
53. March 21, 2003: “Molecular physiology of bicarbonate transport.” Whitney Laboratory, Univ. FL, St. Augustine, FL.
Invited by Professor Paul J. Linser, Ph.D.
54. March 25, 2003: “Flavors of bicarbonate transporters- something old & something new.” NIH, NHLBI: Laboratory of
Kidney and Electrolyte Metabolism. Invited by Mark A. Knepper, Chief.
55. Aug. 17, 2003- Romero, MF. “Mammalian transporter families” for PharmaConference 2003 – Transporters & Drugs.
Aug. 17-21, 2003, Pontresina, Switzerland. Invited by Matthias A. Hediger, PhD- organizer
56. Oct. 23, 2003: “The gasly details of bicarbonate transporters- a tale of 2 families.” Renal Electrolyte Division, Univ.
Pittsburg, Pittsburg, PA. Invited by Thomas R. Kleyman, MD.
57. Nov. 16, 2003 - Romero, MF: “Bicarbonate transporters.” Am. Soc. Nephrol. 2003 Symposium – New Insights to Acid-
Base Physiology. Invited by Jeff Sands, MD, organizer.
2004
58. 2004: “The tricks and disguises of bicarbonate transporters.” Dept. Physiology & Biophysics, Wright State University.
Invited by Drs. Tom Brown & Peter Lauf.
59. April 17, 2004: “In the beginning …. There was the cell.” APS Refresher Course on “Cellular Homeostasis.” Organizer
and Symposium Chairperson.
60. April 17, 2004: “Generation of membrane potential.” APS Refresher Course on “Cellular Homeostasis.” Replacement for
Dr. Steve Wright.
61. May 27, 2004: “Sodium/bicarbonate cotransporters and new bicarbonate transporters in the kidney” for Japanese Society
of Nephrology, May 27, 2004, Utsunomiya, Japan. Invited by Prof. Hitoshi Endou (Kyorin University, Tokyo)- organizer.
62. July 18-25, 2004: “NBCe1 - lessons from soluble domains and evolutionary variation.” Biophysical and Biochemical
Properties of Ion Channels in Epithelia Symposium at Telluride Science Research Center, Telluride, CO. Speaker - Invited
by Drs. Thomas Kleyman and John Cuppoletti.
63. Dec 3, 2004: "Ion, solute and acid-base transport physiology of the Na+
coupled monocarboxylate cotransporter SLC5A8 -
a renal perspective." Abbott Laboratories.
2005
64. Feb 2, 2005: “Bicarbonate and acid-base transporters in acid-base balance.” Dept. Molecular & Cellular Physiology,
University of Cincinnati, Cincinnati, OH. Invited by Dr. Marshall Montrose.
65. Feb 28- March 3, 2005: “Electrogenicity of Cl- and HCO3 transport by the SLC26 transporters” for Novartis (formerly
Ciba) Foundation Symposium "Epithelial anion transport in health and disease: the role of the SLC26 transporters family,"
London, U.K. Invited speaker.
66. March 4, 2005: “Overview of the SLC26 family and associated diseases” for “Epithelial anion transport in health and
disease: the role of the SLC26 transporters family.” Sponcered by Novatris and the Physiology Society. Invited speaker.
67. March 9, 2005: Bicarbonate transporters – old friends and new twists. Dept. Integrative and Molecular Physiology,
University of Michigan at Ann Arbor. Invited by Dr. John Williams.
68. March 28, 2005: “Electrogenic Slc26 Anion ‘Transporters’ – New Molecular and Physiologic Twists for Epithelia” Dept.
Cell Biology & Physiology, University of Pittsburgh. Invited by Dr. Raymond Frizzell.
69. May 2, 2005: “Sodium monocarboxylate cotransporters- Cancer, fish, frogs and solute transport.” Physiology &
Biophysics, CWRU. Invited by Dr. George Dubyak.
70. Aug. 29, 2005: “Bicarbonate transporters – physiology insights from molecular physiology” Dept. Physiology, Tulane
University. Invited by Dr. L. Gabriel Navar. Postponed due to Hurricane Katrina
71. September, 19-22, 2005: “Molecular Physiology of chloride exchangers (SLC4 and SLC26)” for the First
International meeting on Physiology and Pathology of chloride transporters and channels, Soria, Spain. Invited
speaker by Dr. Gerardo Gamba.
72. Fall 2005: “Acid-base transport from humans and fish,” Laboratory of Pharmacology & Chemistry and Laboratory of
Molecular Toxicology, National Institute of Environmental Health Sciences., Research Triangle Park, NC. Invited by Drs.
Leo T. Burka and John Pritchard.
73. Nov 11, 2005, ASN 2005: “Electrogenic Na+
Bicarbonate cotransport” for ASN Symposium “Sodium cotransporter
protein families: Molecular biology and regulation.” Invited speaker by Dr. Mark Zeidel.
74. Dec. 15, 2005: “Na+
Bicarbonate cotransporters: biophysics, evolution and human disease” Dept. Physiology &
Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, MN. Invited by Dr. Gary C. Sieck.
2006
75. Jan. 19, 2006: “Electrogenic Na+
bicarbonate cotransporters in epithelia: physiology, evolution and human disease” Dept.
Anatomy & Physiology, Kansas State University, Manhattan, KS. Invited by Dr. Daniel Marcus.
76. Jan. 27, 2006: “Electrogenic Slc26 Anion ‘Transporters’ – new molecular and physiologic paradigms for epithelia”
Pediatric Pulmonary/CF Research Center Seminar at CWRU, Cleveland, OH. Invted by Dr. Calvin U. Cotton.
MF Romero Curriculum vitae - September 2, 2011 23
77. Feb 20, 2006: “Electrogenic Na+
bicarbonate cotransporters in epithelia: physiology, evolution and human disease,” Dept.
Anatomy & Cell Biology, Univ. Pennsylvania School of Dental Medicine, Philiadelphia, PA. Invited by Dr. Ed Macarak.
78. Feb 23, 2006: “Electrogenic Na+
bicarbonate cotransporters in the kidney and epithelia: physiology, evolution and human
disease.” Nephrology Hypertension Research Conference, CWRU. Invited by Mike Simonson.
79. Nov. 4, 2006; 21st annual Ohio Physiological Society Meeting, Kenyon College, Gambier, OH: “Frogs, fish and flies to
study ion transporters”. Invited by Dr. Chris Gillen.
80. Dec. 19, 2006: “Bicarbonate transporters in the kidney and epithelia: physiology, evolution and human disease.”
Nephrology Grand Rounds, Mayo Clinic. Invited by Vicente Torres, M.D., Ph.D.
2007
81. Jan. 26, 2007: “Electrogenic bicarbonate transporters in the kidney and epithelia: physiology, evolution and human
disease.” Physiology & BME seminar, Mayo Clinic. Invited by Armando Manduca, PhD
82. March 8-11, 2007- APS Professional Skills Course: Making Scientific Presentations: Critical First Skills, Bethesda, MD;
“Designing a Great Poster.”
83. Aug 13, 2007: BioMedical Transporters 2007 Conference, Berne, Switzerland: “NBCe1, the molecular basis of proximal
renal tubular acidosis and glaucoma – the kidney, flies and fish & back again.” Invited by Dr. Matthias A. Hediger.
84. Aug 17, 2007: “Structure & function of bicarbonate transporters- lessons from human disease and evolution” Institute fur
Biophysik, Uni. Stuttgart, Stuttgart, Germany. Invited by Prof. Dr. Stephan Nuβberger.
85. Oct 22, 2007: “Electrogenic bicarbonate transporters in the kidney, eye and epithelia: physiology, evolution & human
disease.” Opthamalogy Grand Rounds, Mayo Clinic.
2008
86. April 2008; EB Featured topic: “Gut of fly, kidney and cake of fish, stomach of man & egg of frog – a witch’s brew of GI
epithelial transport.” Invited by Drs. Didier Merlin and Pradeep Dudeja.
87. June 7, 2008: ROCK SOCIETY 2008 “Slc26 Proteins and Oxalate Transport.”
88. July 11, 2008: “Transporter Biology & Physiology: Romero lab overview.” Physiology & BME seminar series.
89. Sept 5-8, 2008: Epithelial Solute Transporters and Acid-Base Regulation, Le Chateau Montebello, Quebec, Canada “The
SLC26 family of anion exchangers.” Invited by Drs. Steve Perry, Bill Harvey and Nathan Nelson
90. Sept 24-29, 2008: International Congress of Eye Research (ICER), Beijing, China: Physiology & Pharmacology session.
“Slc4 and Slc26 anion transporters in epithelia.” Invited by Alan Marmorstein, PhD
91. Nov. 11, 2008: Boulpaep Symposium- Yale University. “Evolving and shifting anion transport modes with the Slc26
anion “transporters”” Invited by Michael J. Caplan, MD, PhD (Chair, Cell & Molec. Physiology).
2009
92. July 10, 2009: “Transporter Biology & Physiology.” Biochemistry & Molecular Biology- SURF presentation.
93. Sept. 25, 2009: “The Unbearable Lightness of Anion Transporters - the common thread of acidosis, cystic fibrosis,
malaria, glaucoma and kidney stones.” Physiology & BME seminar series.
2010
94. March 19, 2010; “Oxalate transport, kidney stones and Slc26 transporters” Mayo O’Brien Center Research Conference.
Invited by John Lieske, MD, O’Brien Center Director.
95. April 13, 2010; “Microelectrodes to determine ion and solute transport in cells – tools for acidosis, cystic fibrosis, malaria,
glaucoma and kidney stones” Design of Medical Devices Conference, Minneapolis, MN. Invited by Tianhong Cui, PhD.
96. May 17, 2010; Tokyo Institute of Technology. Invited by Dr. Shige Hirose, Dean.
97. May 19, 2010; Laboratory of Biotechnology, Shizuoka University. Invited by Dr. Enoch Park.
98. May 19-21, 2010; Morioka, Japan, 87th Annual Meeting of the Physiological Society of Japan: Invited by Manabu
Kubokawa, MD, PhD, Vice President of Physiological Society of Japan.
99. July 9, 2010; “Epithelial bicarbonate transporters – insights to acidosis, glaucoma, kidney stones and malaria” Physiology
& BME, Mayo Clinic. Invited by Dr. Armando Manduca
100.September 27, 2010; “Oxalate transport, kidney stones and Slc26 transporters” Physiology and Functional Genomics,
University of Florida College of Medicine. Invited by Drs. Debbie Scheuer and Charles Wingo
2011
101.Jan 18, 2011; “Bicarbonate transporters – Models to study kidney disease, glaucoma and parasite control” Biochemistry
and Molecular Biology, Mayo Clinic. Invited by Dr. Mark McNiven
102.March 1, 2011: “In vivo Drosophila model of Ca-oxalate nephrolithiasis - a search for genetic pathophysiology”
Nephrology & Hypertension Grand Rounds.
103.May 20, 2011: “Mayo Translational PKD Center - Model Systems Core” External Advisory Site visit.
104.May 24, 2011; “PhD research funding: illusions, realities and possibilities” Mayo Postdoctoral Association.
105.June 28, 2011: “Epithelial bicarbonate transporters – insights to renal tubular acidosis, glaucoma, cystic fibrosis and
kidney stones” Molecular, Cell & Systems Biology, Univ. Glasgow, UK. Invited by Prof. Julian AT Dow.
MF Romero Curriculum vitae - September 2, 2011 24
106.July 1-4, 2011; “Drosophila model of kidney stones: function and knockdown of Slc26a5 (prestin)” Glasgow conference
honoring Prof. Bill Harvey. Invited by Klaus Beyenbach, PhD.
107.July 3, 2011; “Bicarbonate transporters in the Drosophila and Anopheles” Satelite meeting for Soc. Exp. Biol. Glasgow
conference. Invited by Julian Dow, D.Sc.
108.July 19, 2011; “How we try to understand kidney stone disease” MF Romero and J Lieske. Presentation of Mayo O’Brien
Research Center to Mayo SURF students
109.July 21, 2011; “Flies to humans, kidney disease, cystic fibrosis, glaucoma and malaria: Roles of bicarbonate transporting
membrane proteins” Biochemistry and Molecular Biology seminar. Invited by Jennifer Westendorf
110.July 22, 2011; “Bicarbonate and Solute Transporters in Epithelial – Why Should You Care?” Physiology & biomedical
Engineering Seminar, Mayo Clinic College of Medicine. Invited by Dr. Armando Manduca.
111.July 29, 2011; “O’Brien Urology Center – Pilot Projects and Supplements” O’Brien Urology Research Center update.
112.August 29-30, 2011: “Bicarbonate transporters in the Drosophila and Anopheles: possible models for human disease”
icarbonate transporters Symposium-15 yrs. Aarhaus, Denmark. Invited by Drs. Ebbe Boedtkjer and Christian Aalkjaer.
113.Oct 3, 2011. “Stoned Flies, cystic fibrosis and glaucoma: Roles of bicarbonate transporting membrane proteins”
Physiology & Biophysics, Case Western Reserve University School of Medicine; Invited by Walter F. Boron, MD, PhD
(Chair)
114.Nov 18, 2011: Symposium in honor of Ulrich Hopfer, MD-PhD. Physiology & Biophysics, Case Western Reserve
University School of Medicine; Invited by Walter F. Boron, MD, PhD (Chair)
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