Normal Enzyme Activity

Cuvette 1 2Reaction Mixture (uL) 900 900Normal Serum (uL) 100 75Time (sec) Absorbance Cuv.1 Absorbance Cuv.2

0 0.3 0.215 0.355 0.24530 0.408 0.28845 0.458 0.32360 0.506 0.35775 0.553 0.39490 0.608 0.428

105 0.652 0.461120 0.695 0.497

Absorbance/Min (30-90) 0.2 0.14

dA=E.dC.l E= 6.2dC (mmol/mL/min) 0.032258064516129 0.02258064516129Activity U (umol/min) 0.32258064516129 0.225806451612903

20 30 40 50 60 70 80 90 1000

0.1

0.2

0.3

0.4

0.5

0.6

0.7

Absorbance vs Time (Cuv.1)

Time

Abso

rban

ce

20 30 40 50 60 70 80 90 1000

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0.45

Absorbance vs Time (Cuv.2)

Time

Abso

rban

ce

20 30 40 50 60 70 80 90 1000

0.1

0.2

0.3

0.4

0.5

0.6

0.7

Absorbance vs Time (Cuv.1)

Time

Abso

rban

ce

20 30 40 50 60 70 80 90 1000

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0.45

Absorbance vs Time (Cuv.2)

Time

Abso

rban

ce