MBLG Normal Serum
2
Normal Enzyme Activity Cuvette 1 2 Reaction Mixture (uL) 900 900 Normal Serum (uL) 100 75 Time (sec) Absorbance Cuv.1 Absorbance Cuv.2 0 0.3 0.2 15 0.355 0.245 30 0.408 0.288 45 0.458 0.323 60 0.506 0.357 75 0.553 0.394 90 0.608 0.428 105 0.652 0.461 120 0.695 0.497 Absorbance/Min (30-90) 0.2 0.14 dA=E.dC.l E= 6.2 dC (mmol/mL/min) 0.032258064516129 0.02258064516129 Activity U (umol/min) 0.32258064516129 0.225806451612903
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LDH assay report
Transcript of MBLG Normal Serum
Normal Enzyme Activity
Cuvette 1 2Reaction Mixture (uL) 900 900Normal Serum (uL) 100 75Time (sec) Absorbance Cuv.1 Absorbance Cuv.2
0 0.3 0.215 0.355 0.24530 0.408 0.28845 0.458 0.32360 0.506 0.35775 0.553 0.39490 0.608 0.428
105 0.652 0.461120 0.695 0.497
Absorbance/Min (30-90) 0.2 0.14
dA=E.dC.l E= 6.2dC (mmol/mL/min) 0.032258064516129 0.02258064516129Activity U (umol/min) 0.32258064516129 0.225806451612903
20 30 40 50 60 70 80 90 1000
0.1
0.2
0.3
0.4
0.5
0.6
0.7
Absorbance vs Time (Cuv.1)
Time
Abso
rban
ce
20 30 40 50 60 70 80 90 1000
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
Absorbance vs Time (Cuv.2)
Time
Abso
rban
ce
20 30 40 50 60 70 80 90 1000
0.1
0.2
0.3
0.4
0.5
0.6
0.7
Absorbance vs Time (Cuv.1)
Time
Abso
rban
ce
20 30 40 50 60 70 80 90 1000
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
Absorbance vs Time (Cuv.2)
Time
Abso
rban
ce
