Lecture 12HPLC

Lecture 12HPLC

GC setup

LC setup

LC Injector

Back to Van Deempter

H ∞ A + C

H ∞ + dp

2DMP

Ce dp

Back to Van Deempter

Mobile Phase

H ∞ + dp

2DMP

Ce dp

LC ColumnsParticle Size (dp)

Dong, M. Today’s Chemist at Work. 2000, 9(2), 46-48.

H ∞ + dp

2DMP

Ce dp

LC ColumnsParticle Size (dp)

H ∞ + dp

2DMP

Ce dp

LC ColumnsTortuosity ()

Ascentis Express, Supelco, technical information

H ∞ + dp

2DMP

Ce dp

Injector/column interaction

Types of LC columns

C18 C8

Column elution

Size exclusion chromatography