Lecture 12 HPLC
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Lecture 12 HPLC
description
Lecture 12 HPLC. Lecture 12 HPLC. GC setup. LC setup. LC Injector. Back to Van Deempter. A +. ∞. H . C . Back to Van Deempter. d p 2 . +. C e d p. ∞. H . . D MP. Mobile Phase. d p 2 . +. C e d p. ∞. H . . D MP. LC Columns Particle Size (d p ). d p 2 . +. - PowerPoint PPT Presentation
Transcript of Lecture 12 HPLC
Lecture 12HPLC
Lecture 12HPLC
GC setup
LC setup
LC Injector
Back to Van Deempter
H ∞ A + C
H ∞ + dp
2DMP
Ce dp
Back to Van Deempter
Mobile Phase
H ∞ + dp
2DMP
Ce dp
LC ColumnsParticle Size (dp)
Dong, M. Today’s Chemist at Work. 2000, 9(2), 46-48.
H ∞ + dp
2DMP
Ce dp
LC ColumnsParticle Size (dp)
H ∞ + dp
2DMP
Ce dp
LC ColumnsTortuosity ()
Ascentis Express, Supelco, technical information
H ∞ + dp
2DMP
Ce dp
Injector/column interaction
Types of LC columns
C18 C8
Column elution
Size exclusion chromatography
![November 12, 2018 Pharmaceuticals and Medical Devices Agency · identification (HPLC and ultraviolet-visible spectrum [UV/VIS]), purity (enantiomers [HPLC]), uniformity of dosage](https://static.fdocuments.us/doc/165x107/5f4351075c90a95d9d55a2b1/november-12-2018-pharmaceuticals-and-medical-devices-agency-identification-hplc.jpg)
