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DissolvedOxygenInstruction Manual Code7414/5860WaterQualityTestKit
http://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kithttp://www.promagenviro.com/Dissolved-Oxygen-Test-Kit7/28/2019 LaMotte 5860 Dissolved Oxygen Liquid Acid Version Kit Instructions
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INTRODUCTION
Aquaticanimalsneeddissolvedoxygentolive.Fish,invertebrates,plants,andaerobicbacteriaallrequireoxygenforrespiration.Oxygendissolvesreadilyintowaterfromtheatmosphereuntilthewaterissaturated.Oncedissolvedinthewater,theoxygendiffusesveryslowlyanddistributiondependsonthemovementoftheaeratedwater.Oxygenisalsoproducedbyaquaticplants,algae,andphytoplanktonasaby-productofphotosynthesis.Theamountofoxygenrequiredvariesaccordingtospeciesandstageoflife.DissolvedOxygenlevelsbelow3ppmarestressfultomostaquaticorganisms.DissolvedOxygenlevelsbelow2or1ppmwillnotsupportfish.Levelsof5to6ppmareusuallyrequiredforgrowthandactivity.ThistestkitusestheazidemodificationoftheWinklermethodfordeterminingdissolvedoxygen.TABLE OF CONTENTS
PageKitContents....................................................................... 2TestProcedure
Part1: CollectingaWaterSample......................................3Part2: AddingtheReagents............................................... 4Part3: Titration................................................................... 5
EPACompliance................................................................. 8DissolvedOxygenFactSheet............................................. 10GeneralSafetyPrecautions................................................13UseProperAnalyticalTechniques.....................................14MaterialSafetyDataSheets.............................................. 15KitDiagrams..................................................................... 21ShortFormInstructions...................................... BackCover
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KIT CONTENTS
QUANTITY CONTENTS CODE
30mL *ManganousSulfateSolution *4167-G30mL *AlkalinePotassiumIodideAzide *7166-G50g *SulfamicAcidPowder(7414Kit) *6286-H30mL *SulfuricAcid,1:1(5860Kit) *6141WT-G60mL *SodiumThiosulfate,0.025N *4169-H30mL StarchIndicatorSolution 4170WT-G1 Spoon,1.0g,plastic(7414Kit) 06971 DirectReadingTitrator 03771 TestTube,5-10-12.9-15-20-25mL, 0608
glass,w/cap1 WaterSamplingBottle,60mL,glass 0688-DO*WARNING: Reagentsmarkedwitha*areconsideredhazardoussubstances.TovieworprintaMaterialSafetyDataSheet(MSDS)forthesereagentsseeMSDSCDorourwebsite.Toobtainaprintedcopy,contactusbye-mail,
phone
or
fax..
Toorderindividualreagentsortestkitcomponents,usethespecifiedcodenumbers.
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TEST PROCEDURE
PART 1 - COLLECTING THE WATER SAMPLE
6.
RinsetheWaterSamplingBottle(0688-DO)withthesamplewater.
1.
Removethecapandallowthebottletofill.
3.Tapthesidesofthebottletodislodgeanyairbubbles.
4.Tightlycapthebottle,andsubmergeittothedesireddepth.
2.
Replacethecapwhilethebottleisstillsubmerged.
5.Retrievethebottleandmakesurethatnoairbubblesaretrappedinside.
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TEST PROCEDURE
PART 2 - ADDING THE REAGENTS
Becarefulnottointroduceairintothesamplewhileaddingthereagents.
Atthispointthesamplehas
ForKitCode5860:Add8dropsof*SulfuricAcid,1:1(6141WT).
Capandgentlyinvertthebottletomixthecontentsuntilthe
ForKitCode7414:Immediatelyusethe1.0gspoon(0697)toaddonelevelmeasureof*Sulfamic
AcidPowder(6286).OR
NOTE: 2.
3.Allowtheprecipitatetosettlebelowtheshoulderofthebottle.
5.
6. NOTE:precipitateandthereagenthavetotallydissolved.Thesolutionwillbeclearyellowtoorangeifthesamplecontainsdissolvedoxygen.
been"fixed"andcontactbetweenthesampleandtheatmospherewillnotaffectthetestresult.Samplesmaybeheldatthispointandtitratedlater.
Immediatelyadd8dropsof*ManganousSulfateSolution(4167)
Add 8dropsof*AlkalinePotassiumIodide
Azide(7166).
AND
Removethecapfromthebottle.1.
4.Capthebottleandmixbyinvertingseveraltimes.Aprecipitatewillform.
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TEST PROCEDURE
PART 3 - THE TITRATION
Fillthetitrationtube(0608)tothe20mLlinewiththefixedsample.Capthetube.
2.DepressplungeroftheTitrator(0377).
1.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
00.10.20.30.40.50.60.70.80.91.0
IfsmallairbubblesappearintheTitratorbarrel,expelthembypartiallyfillingthebarrelandpumpingthetitrationsolutionbackintothereagentcontainer.Repeatuntilbubbledisappears.
NOTE:
InserttheTitratorintothepluginthetopofthe*SodiumThiosulfate,0.025N(4169)titratingsolution.
3.
5.
Turnthe
bottle
uprightandremovetheTitrator. NOTE:Ifthe sample is a very
paleyellow,gotoStep9.
4.Invertthebottleandslowlywithdrawtheplungeruntilthelargeringontheplungerisoppositethezero(0)lineonthescale.
00.10.20.30.40.50.60.70.80.91.0
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
0
0.10.20.30.40.50.60.70.80.91.0
0
0.10.20.30.40.50.60.70.80.91.0
continued...5
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TEST PROCEDURE
9.
6.
Continuetitratinguntilthebluecolordisappearsandthesolutionbecomescolorless.
CarefullyremovetheTitratorandcap.DonottodisturbtheTitratorplunger.
Add8dropsofStarchIndicatorSolution(4170WT).Thesampleshouldturnblue.
8.
11.10.Capthetitrationtube.InsertthetipoftheTitratorintotheopeningofthetitrationtubecap.
InsertthetipoftheTitratorintotheopeningofthetitrationtubecap.
Slowlydepresstheplungertodispensethetitratingsolutionuntiltheyellow-browncolorchangestoaverypaleyellow.Gentlyswirlthetubeduringthetirationtomixthecontents.
7.
12.ReadthetestresultdirectlyfromthescalewherethelargeringontheTitratormeetstheTitratorbarrel. RecordasppmDissolvedOxygen.EachminordivisionontheTitratorscaleequals0.2ppm.
0
0.10.20.30.40.50.60.70.80.91.0
0
0.10.20.30.40.50.60.70.80.91.0
0
0.10.20.30.40.50.60.70.80.91.0
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
4.05.0
Result:4.0ppm
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TEST PROCEDURE
NOTE:
Iftheplungerringreachesthebottomlineonthescale(10ppm)beforetheendpointcolorchangeoccurs,refilltheTitratorandcontinuethetitration.Includethevalueoftheoriginalamountofreagentdispensed(10ppm)whenrecordingthetestresult.
Whentestingiscomplete,discardtitratingsolutioninTitrator.RinseTitratorandtitrationtubethoroughly.DONOTremoveplungeroradaptertip.
NOTE:0
0.10.20.30.40.50.60.70.80.91.0
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EPA COMPLIANCE
ToqualifyasanEPAacceptedtest,andtoachievethegreatestaccuracy,theSodiumThiosulfateSolution,0.025N(4169)mustbestandardizeddaily.ThisprocedurefollowsStandardMethodsfortheExaminationofWaterandWastewater.Numbersin()areforLaMotteproducts.TheseproductsarenotincludedinthiskitbutcanbeorderedfromLaMotteCompanybyusingthespecifiedcodenumber.
4.
5.
2.
3.
1.
6.
Add 2 drops ofSulfuricAcid,5N(8517WT).
Usea10
mL
graduated
cylinder(0416)toadd15mLofDeionizedWater(5115)tothetitrationtube(0608).
UseaDirectReadingTitrator,0-1Range(1.0mLcapacity)(0376)toadd2mLofPotassiumBi-iodate(7346).
Usethe0.1gspoon(0699)toadd0.2gPotassiumIodideCrystals(6809).
FillanotherDirectReadingTitrator(0376)withSodiumThiosulfateSolution,0.025N(4169).
Swirltomix.Solutionwillturnyellowishbrown.
0
0.10.20.30.40.50.60.70.80.91.0
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
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EPA COMPLIANCE
Whilegentlyswirlingthetube,addSodiumThiosulfate,0.025Nuntilthecolorfadestopaleyellow.ItwillbenecessarytorefilltheDirectReadingTitrator.
7.0
0.10.20.30.40.50.60.70.80.91.0
8.Add3dropsofStarchIndicatorSolution(4170WT).Thesolutionwillturnblue.
9.ContinueaddingSodiumThiosulfate,0.025Nuntilthebluecolordisappearsandthesolutioniscolorless.
10.ReadthetestresultdirectlyfromthescalewherethelargeringontheTitratormeetstheTitratorbarrel.Includethevalueoftheoriginalamountdispensed(1mL).Ifthereadingis2.0+/-0.1mL,theSodiumThiosulfate,0.025N(4169)issatisfactory.If not,discardandreplacewithnewreagent.
2.03.0
Result:2.0ppm
0
0.10.20.30.40.50.60.70.80.91.0
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DISSOLVED OXYGEN FACT SHEET
Oxygeniscriticaltothesurvivalofaquaticplantsandanimals,andashortageofdissolvedoxygenisnotonlyasignofpollution,itisharmfultofish.Someaquaticspeciesaremoresensitivetooxygendepletionthanothers,butsomegeneralguidelinestoconsiderwhenanalyzingtestresultsare:
56ppm Sufficientformostspecies
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WHERE DOES THE OXYGEN GO?Onceinthewater,oxygenisusedbytheaquaticlife.Fishandotheraquaticanimalsneedoxygentobreatheorrespire.Oxygenisalsoconsumedbybacteriatodecay,ordecompose,deadplantsandanimals.Respiration
Allanimals,whetheronlandorunderwater,needoxygentorespire,growandsurvive.Plantsandanimalsrespirethroughoutthenightandday,consumingoxygenandproducingcarbondioxide,whichisthenusedbyplantsduringphotosynthesis.Decomposition
Allplantandanimalwasteeventuallydecomposes,whetheritisfromlivinganimalsordeadplantsandanimals.Inthedecompositionprocess,bacteriauseoxygentooxidize,orchemicallyalter,thematerialtobreakitdowntoitscomponentparts.Someaquaticsystemsmayundergoextremeamountsofoxidation,leavingnooxygenforthelivingorganisms,whicheventuallyleaveorsuffocate.OTHER FACTORSTheoxygenlevelofawatersystemisnotonlydependentonproductionandconsumption.Manyotherfactorsworktogethertodeterminethepotentialoxygenlevel,including:
Salt
vs.
fresh
water
- Fresh
water
can
hold
more
oxygen
than
salt
water.
Temperature- Coldwatercanholdmoreoxygenthanwarmwater. Atmosphericpressure(Altitude)- Thegreatertheatmospheric
pressurethemoreoxygenthewaterwillhold.TESTING DISSOLVED OXYGENDissolvedoxygenisoftentestedusingtheAzidemodificationoftheWinklermethod.
When
testing
dissolved
oxygen
it
is
critical
not
to
introduce
additionaloxygenintothesample.Manypeopleavoidthisproblembyfillingthesamplebottleallthewayandallowingthewatertooverflowforoneminutebeforecapping.ThefirststepinaDOtitrationistheadditionofManganousSulfateSolution(4167)andAlkalinePotassiumIodideAzideSolution(7166).Thesereagentsreacttoformawhiteprecipitate,orfloc,ofmanganoushydroxide,Mn(OH)2.Chemically,thisreactioncanbewrittenas:
MnSO4 + 2KOH Mn(OH)2 + K2SO4Manganous + Potassium Manganous + Potassium
Sulfate Hydroxide Hydroxide Sulfate
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Immediatelyuponformationoftheprecipitate,theoxygeninthewateroxidizesanequivalentamountofthemanganoushydroxidetobrown-coloredmanganichydroxide.Foreverymoleculeofoxygeninthewater,fourmoleculesofmanganoushydroxideareconvertedtomanganichydroxide.Chemically,thisreactioncanbewrittenas:
4Mn(OH)2 + O2 + 2H2O 4Mn(OH)3Manganous + Oxygen + Water ManganicHydroxide Hydroxide
Afterthebrownprecipitateisformed,astrongacid,suchasSulfamicAcidPowder(6286)orSulfuricAcid,1:1(6141)isaddedtothesample.Theacidconvertsthemanganichydroxidetomanganicsulfate.Atthispointthesampleisconsideredfixedandconcernforadditionaloxygenbeingintroducedintothesampleisreduced.Chemically,thisreactioncanbewrittenas:
2Mn(OH)3 + 3H2SO4 Mn2(SO4)3 + 6H2OManganic + Sulfuric Manganic + Water
Hydroxide Acid SulfateSimultaneously,iodinefromthepotassiumiodideintheAlkalinePotassiumIodideAzideSolutionisoxidizedbymanganicsulfate,releasingfreeiodineintothewater.Sincethemanganicsulfateforthisreactioncomesfromthereactionbetweenthemanganoushydroxideandoxygen,theamountofiodinereleasedisdirectlyproportionaltotheamountofoxygenpresentintheoriginalsample.Thereleaseoffreeiodineisindicatedbythesampleturningayellow-browncolor.Chemically,thisreactioncanbewrittenas:
2KI + +Mn2(SO4)3 + 2MnSO4 K2SO4 I2Manganic + Potassium Manganous+ Potassium +Iodine
Sulfate Iodide Sulfate SulfateThefinalstageintheWinklertitrationistheadditionofsodiumthiosulfate.Thesodiumthiosulfatereactswiththefreeiodinetoproducesodiumiodide.Whenalloftheiodinehasbeenconvertedthesamplechangesfromyellow-browntocolorless.Oftenastarchindicatorisaddedtoenhancethefinalendpoint.Chemically,thisreactioncanbewrittenas:
I2 2Na2S2O3 + Na2S4O6 + 2NaISodium + Iodine Sodium + Sodium
Thiosulfate Tetrathionate Iodide
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GENERAL SAFETY PRECAUTIONS
Avoidcontactbetweenreagentchemicalsandskin,eyes,nose,andmouth.
Intheeventofanaccidentorsuspectedpoisoning,immediatelycallthePoisonCenterphonenumberinthefrontofyourlocaltelephonedirectoryorcallaphysician.AdditionalinformationforallLaMottereagentsisavailableintheUnitedStates,Canada,PuertoRico,andtheUSVirginIslandsfromChem-Telbycalling1-800-255-3924.Forotherareas,call813-248-0585collecttocontactChem-TelsInternationalaccessnumber.Eachreagentcanbeidentifiedbythefourdigitnumberlistedontheupperleftcornerofthereagentlabel,inthecontentslistandinthetestprocedures.
7.
1.Storethetestkitinacool,dryarea.
Wearsafetyglasseswhenperformingtestprocedures.
Readallinstructionsandnoteprecautionsbeforeperformingthetestprocedure.
Instruction
Manual
2.
Keepallequipmentandreagentchemicalsoutofthereachofyoungchildren.
Material
Safety
Data
SheetReadthelabelsonallreagentbottles.Notewarningsandfirstaidinformation.ReadallMaterialSafetyDataSheets.
3. 4.
5. 6.
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USE PROPER ANALYTICAL TECHNIQUES
Usetesttubecapsorstoppers,notyourfingers,tocovertubesduringshakingormixing.
Tightlycloseallcontainersimmediatelyafteruse.Donotinterchangecapsfromcontainers.
1.
Avoidprolongedexposureofequipmentandreagentstodirectsunlight.Protectreagents
from
extremesoftemperature.
2.
Wipeupanyreagentchemicalspillsimmediately.3. 4.
5. 6.
Holddropperbottlesverticallyupside-down,andnotatanangle,whendispensingareagent.Squeezethebottlegentlytodispensethereagentonedropatatime.
Thoroughlyrinsetesttubesbeforeandaftereachtest.
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KIT DIAGRAMS
DISSOLVED OXYGEN KIT CODE 7414
Instructions4169-H
0377
6286-H
4167-G
4170WT-G 0608Tube
7166-G0688-DO
0697
DISSOLVED OXYGEN KIT CODE 5860
Instructions4169-H
0377
6141WT-G
4167-G
4170WT-G 0608Tube
7166-G0688-DO
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SHORT FORM INSTRUCTIONS
Readallinstructionsbeforeperformingtest.Usethisguideasaquickreference.1. FillWaterSamplingBottle(0688-DO).2. Add8dropsof*ManganousSulfateSolution(4167).3. Add8dropsof*AlkalinePotassiumIodideAzide(7166).4. Capandmix.5. Allowprecipitatetosettle.6. Usethe1.0gspoontoadd*SulfamicAcidPowder(6286)or
add8dropsofSulfuricAcid,1:1(6141WT).7. Capandmixuntilreagentandprecipitatedissolve.8. Filltesttube(0608)tothe20mLline.9. FillTitratorwith*SodiumThiosulfate,0.025N(4169).10. Titrateuntilsamplecolorispaleyellow.DONOTDISTURB
TITRATOR.11. Add8dropsofStarchIndicator(4170WT).12. Continuetitrationuntilbluecolorjustdisappearsandsolution
iscolorless.13. ReadresultinppmDissolvedOxygen.
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