LaMotte 5860 Dissolved Oxygen Liquid Acid Version Kit Instructions

7/28/2019 LaMotte 5860 Dissolved Oxygen Liquid Acid Version Kit Instructions

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DissolvedOxygenInstruction Manual Code7414/5860WaterQualityTestKit
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INTRODUCTION

Aquaticanimalsneeddissolvedoxygentolive.Fish,invertebrates,plants,andaerobicbacteriaallrequireoxygenforrespiration.Oxygendissolvesreadilyintowaterfromtheatmosphereuntilthewaterissaturated.Oncedissolvedinthewater,theoxygendiffusesveryslowlyanddistributiondependsonthemovementoftheaeratedwater.Oxygenisalsoproducedbyaquaticplants,algae,andphytoplanktonasaby-productofphotosynthesis.Theamountofoxygenrequiredvariesaccordingtospeciesandstageoflife.DissolvedOxygenlevelsbelow3ppmarestressfultomostaquaticorganisms.DissolvedOxygenlevelsbelow2or1ppmwillnotsupportfish.Levelsof5to6ppmareusuallyrequiredforgrowthandactivity.ThistestkitusestheazidemodificationoftheWinklermethodfordeterminingdissolvedoxygen.TABLE OF CONTENTS

PageKitContents....................................................................... 2TestProcedure

Part1: CollectingaWaterSample......................................3Part2: AddingtheReagents............................................... 4Part3: Titration................................................................... 5

EPACompliance................................................................. 8DissolvedOxygenFactSheet............................................. 10GeneralSafetyPrecautions................................................13UseProperAnalyticalTechniques.....................................14MaterialSafetyDataSheets.............................................. 15KitDiagrams..................................................................... 21ShortFormInstructions...................................... BackCover

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KIT CONTENTS

QUANTITY CONTENTS CODE

30mL *ManganousSulfateSolution *4167-G30mL *AlkalinePotassiumIodideAzide *7166-G50g *SulfamicAcidPowder(7414Kit) *6286-H30mL *SulfuricAcid,1:1(5860Kit) *6141WT-G60mL *SodiumThiosulfate,0.025N *4169-H30mL StarchIndicatorSolution 4170WT-G1 Spoon,1.0g,plastic(7414Kit) 06971 DirectReadingTitrator 03771 TestTube,5-10-12.9-15-20-25mL, 0608

glass,w/cap1 WaterSamplingBottle,60mL,glass 0688-DO*WARNING: Reagentsmarkedwitha*areconsideredhazardoussubstances.TovieworprintaMaterialSafetyDataSheet(MSDS)forthesereagentsseeMSDSCDorourwebsite.Toobtainaprintedcopy,contactusbye-mail,

phone

or

fax..

Toorderindividualreagentsortestkitcomponents,usethespecifiedcodenumbers.

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TEST PROCEDURE

PART 1 - COLLECTING THE WATER SAMPLE

6.

RinsetheWaterSamplingBottle(0688-DO)withthesamplewater.

1.

Removethecapandallowthebottletofill.

3.Tapthesidesofthebottletodislodgeanyairbubbles.

4.Tightlycapthebottle,andsubmergeittothedesireddepth.

2.

Replacethecapwhilethebottleisstillsubmerged.

5.Retrievethebottleandmakesurethatnoairbubblesaretrappedinside.

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TEST PROCEDURE

PART 2 - ADDING THE REAGENTS

Becarefulnottointroduceairintothesamplewhileaddingthereagents.

Atthispointthesamplehas

ForKitCode5860:Add8dropsof*SulfuricAcid,1:1(6141WT).

Capandgentlyinvertthebottletomixthecontentsuntilthe

ForKitCode7414:Immediatelyusethe1.0gspoon(0697)toaddonelevelmeasureof*Sulfamic

AcidPowder(6286).OR

NOTE: 2.

3.Allowtheprecipitatetosettlebelowtheshoulderofthebottle.

5.

6. NOTE:precipitateandthereagenthavetotallydissolved.Thesolutionwillbeclearyellowtoorangeifthesamplecontainsdissolvedoxygen.

been"fixed"andcontactbetweenthesampleandtheatmospherewillnotaffectthetestresult.Samplesmaybeheldatthispointandtitratedlater.

Immediatelyadd8dropsof*ManganousSulfateSolution(4167)

Add 8dropsof*AlkalinePotassiumIodide

Azide(7166).

AND

Removethecapfromthebottle.1.

4.Capthebottleandmixbyinvertingseveraltimes.Aprecipitatewillform.

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TEST PROCEDURE

PART 3 - THE TITRATION

Fillthetitrationtube(0608)tothe20mLlinewiththefixedsample.Capthetube.

2.DepressplungeroftheTitrator(0377).

1.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

00.10.20.30.40.50.60.70.80.91.0

IfsmallairbubblesappearintheTitratorbarrel,expelthembypartiallyfillingthebarrelandpumpingthetitrationsolutionbackintothereagentcontainer.Repeatuntilbubbledisappears.

NOTE:

InserttheTitratorintothepluginthetopofthe*SodiumThiosulfate,0.025N(4169)titratingsolution.

3.

5.

Turnthe

bottle

uprightandremovetheTitrator. NOTE:Ifthe sample is a very

paleyellow,gotoStep9.

4.Invertthebottleandslowlywithdrawtheplungeruntilthelargeringontheplungerisoppositethezero(0)lineonthescale.

00.10.20.30.40.50.60.70.80.91.0

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

0

0.10.20.30.40.50.60.70.80.91.0

0

0.10.20.30.40.50.60.70.80.91.0

continued...5


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TEST PROCEDURE

9.

6.

Continuetitratinguntilthebluecolordisappearsandthesolutionbecomescolorless.

CarefullyremovetheTitratorandcap.DonottodisturbtheTitratorplunger.

Add8dropsofStarchIndicatorSolution(4170WT).Thesampleshouldturnblue.

8.

11.10.Capthetitrationtube.InsertthetipoftheTitratorintotheopeningofthetitrationtubecap.

InsertthetipoftheTitratorintotheopeningofthetitrationtubecap.

Slowlydepresstheplungertodispensethetitratingsolutionuntiltheyellow-browncolorchangestoaverypaleyellow.Gentlyswirlthetubeduringthetirationtomixthecontents.

7.

12.ReadthetestresultdirectlyfromthescalewherethelargeringontheTitratormeetstheTitratorbarrel. RecordasppmDissolvedOxygen.EachminordivisionontheTitratorscaleequals0.2ppm.

0

0.10.20.30.40.50.60.70.80.91.0

0

0.10.20.30.40.50.60.70.80.91.0

0

0.10.20.30.40.50.60.70.80.91.0

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

4.05.0

Result:4.0ppm

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TEST PROCEDURE

NOTE:

Iftheplungerringreachesthebottomlineonthescale(10ppm)beforetheendpointcolorchangeoccurs,refilltheTitratorandcontinuethetitration.Includethevalueoftheoriginalamountofreagentdispensed(10ppm)whenrecordingthetestresult.

Whentestingiscomplete,discardtitratingsolutioninTitrator.RinseTitratorandtitrationtubethoroughly.DONOTremoveplungeroradaptertip.

NOTE:0

0.10.20.30.40.50.60.70.80.91.0

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EPA COMPLIANCE

ToqualifyasanEPAacceptedtest,andtoachievethegreatestaccuracy,theSodiumThiosulfateSolution,0.025N(4169)mustbestandardizeddaily.ThisprocedurefollowsStandardMethodsfortheExaminationofWaterandWastewater.Numbersin()areforLaMotteproducts.TheseproductsarenotincludedinthiskitbutcanbeorderedfromLaMotteCompanybyusingthespecifiedcodenumber.

4.

5.

2.

3.

1.

6.

Add 2 drops ofSulfuricAcid,5N(8517WT).

Usea10

mL

graduated

cylinder(0416)toadd15mLofDeionizedWater(5115)tothetitrationtube(0608).

UseaDirectReadingTitrator,0-1Range(1.0mLcapacity)(0376)toadd2mLofPotassiumBi-iodate(7346).

Usethe0.1gspoon(0699)toadd0.2gPotassiumIodideCrystals(6809).

FillanotherDirectReadingTitrator(0376)withSodiumThiosulfateSolution,0.025N(4169).

Swirltomix.Solutionwillturnyellowishbrown.

0

0.10.20.30.40.50.60.70.80.91.0

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

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EPA COMPLIANCE

Whilegentlyswirlingthetube,addSodiumThiosulfate,0.025Nuntilthecolorfadestopaleyellow.ItwillbenecessarytorefilltheDirectReadingTitrator.

7.0

0.10.20.30.40.50.60.70.80.91.0

8.Add3dropsofStarchIndicatorSolution(4170WT).Thesolutionwillturnblue.

9.ContinueaddingSodiumThiosulfate,0.025Nuntilthebluecolordisappearsandthesolutioniscolorless.

10.ReadthetestresultdirectlyfromthescalewherethelargeringontheTitratormeetstheTitratorbarrel.Includethevalueoftheoriginalamountdispensed(1mL).Ifthereadingis2.0+/-0.1mL,theSodiumThiosulfate,0.025N(4169)issatisfactory.If not,discardandreplacewithnewreagent.

2.03.0

Result:2.0ppm

0

0.10.20.30.40.50.60.70.80.91.0

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DISSOLVED OXYGEN FACT SHEET

Oxygeniscriticaltothesurvivalofaquaticplantsandanimals,andashortageofdissolvedoxygenisnotonlyasignofpollution,itisharmfultofish.Someaquaticspeciesaremoresensitivetooxygendepletionthanothers,butsomegeneralguidelinestoconsiderwhenanalyzingtestresultsare:

56ppm Sufficientformostspecies


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WHERE DOES THE OXYGEN GO?Onceinthewater,oxygenisusedbytheaquaticlife.Fishandotheraquaticanimalsneedoxygentobreatheorrespire.Oxygenisalsoconsumedbybacteriatodecay,ordecompose,deadplantsandanimals.Respiration

Allanimals,whetheronlandorunderwater,needoxygentorespire,growandsurvive.Plantsandanimalsrespirethroughoutthenightandday,consumingoxygenandproducingcarbondioxide,whichisthenusedbyplantsduringphotosynthesis.Decomposition

Allplantandanimalwasteeventuallydecomposes,whetheritisfromlivinganimalsordeadplantsandanimals.Inthedecompositionprocess,bacteriauseoxygentooxidize,orchemicallyalter,thematerialtobreakitdowntoitscomponentparts.Someaquaticsystemsmayundergoextremeamountsofoxidation,leavingnooxygenforthelivingorganisms,whicheventuallyleaveorsuffocate.OTHER FACTORSTheoxygenlevelofawatersystemisnotonlydependentonproductionandconsumption.Manyotherfactorsworktogethertodeterminethepotentialoxygenlevel,including:

Salt

vs.

fresh

water

- Fresh

water

can

hold

more

oxygen

than

salt

water.

Temperature- Coldwatercanholdmoreoxygenthanwarmwater. Atmosphericpressure(Altitude)- Thegreatertheatmospheric

pressurethemoreoxygenthewaterwillhold.TESTING DISSOLVED OXYGENDissolvedoxygenisoftentestedusingtheAzidemodificationoftheWinklermethod.

When

testing

dissolved

oxygen

it

is

critical

not

to

introduce

additionaloxygenintothesample.Manypeopleavoidthisproblembyfillingthesamplebottleallthewayandallowingthewatertooverflowforoneminutebeforecapping.ThefirststepinaDOtitrationistheadditionofManganousSulfateSolution(4167)andAlkalinePotassiumIodideAzideSolution(7166).Thesereagentsreacttoformawhiteprecipitate,orfloc,ofmanganoushydroxide,Mn(OH)2.Chemically,thisreactioncanbewrittenas:

MnSO4 + 2KOH Mn(OH)2 + K2SO4Manganous + Potassium Manganous + Potassium

Sulfate Hydroxide Hydroxide Sulfate

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Immediatelyuponformationoftheprecipitate,theoxygeninthewateroxidizesanequivalentamountofthemanganoushydroxidetobrown-coloredmanganichydroxide.Foreverymoleculeofoxygeninthewater,fourmoleculesofmanganoushydroxideareconvertedtomanganichydroxide.Chemically,thisreactioncanbewrittenas:

4Mn(OH)2 + O2 + 2H2O 4Mn(OH)3Manganous + Oxygen + Water ManganicHydroxide Hydroxide

Afterthebrownprecipitateisformed,astrongacid,suchasSulfamicAcidPowder(6286)orSulfuricAcid,1:1(6141)isaddedtothesample.Theacidconvertsthemanganichydroxidetomanganicsulfate.Atthispointthesampleisconsideredfixedandconcernforadditionaloxygenbeingintroducedintothesampleisreduced.Chemically,thisreactioncanbewrittenas:

2Mn(OH)3 + 3H2SO4 Mn2(SO4)3 + 6H2OManganic + Sulfuric Manganic + Water

Hydroxide Acid SulfateSimultaneously,iodinefromthepotassiumiodideintheAlkalinePotassiumIodideAzideSolutionisoxidizedbymanganicsulfate,releasingfreeiodineintothewater.Sincethemanganicsulfateforthisreactioncomesfromthereactionbetweenthemanganoushydroxideandoxygen,theamountofiodinereleasedisdirectlyproportionaltotheamountofoxygenpresentintheoriginalsample.Thereleaseoffreeiodineisindicatedbythesampleturningayellow-browncolor.Chemically,thisreactioncanbewrittenas:

2KI + +Mn2(SO4)3 + 2MnSO4 K2SO4 I2Manganic + Potassium Manganous+ Potassium +Iodine

Sulfate Iodide Sulfate SulfateThefinalstageintheWinklertitrationistheadditionofsodiumthiosulfate.Thesodiumthiosulfatereactswiththefreeiodinetoproducesodiumiodide.Whenalloftheiodinehasbeenconvertedthesamplechangesfromyellow-browntocolorless.Oftenastarchindicatorisaddedtoenhancethefinalendpoint.Chemically,thisreactioncanbewrittenas:

I2 2Na2S2O3 + Na2S4O6 + 2NaISodium + Iodine Sodium + Sodium

Thiosulfate Tetrathionate Iodide

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GENERAL SAFETY PRECAUTIONS

Avoidcontactbetweenreagentchemicalsandskin,eyes,nose,andmouth.

Intheeventofanaccidentorsuspectedpoisoning,immediatelycallthePoisonCenterphonenumberinthefrontofyourlocaltelephonedirectoryorcallaphysician.AdditionalinformationforallLaMottereagentsisavailableintheUnitedStates,Canada,PuertoRico,andtheUSVirginIslandsfromChem-Telbycalling1-800-255-3924.Forotherareas,call813-248-0585collecttocontactChem-TelsInternationalaccessnumber.Eachreagentcanbeidentifiedbythefourdigitnumberlistedontheupperleftcornerofthereagentlabel,inthecontentslistandinthetestprocedures.

7.

1.Storethetestkitinacool,dryarea.

Wearsafetyglasseswhenperformingtestprocedures.

Readallinstructionsandnoteprecautionsbeforeperformingthetestprocedure.

Instruction

Manual

2.

Keepallequipmentandreagentchemicalsoutofthereachofyoungchildren.

Material

Safety

Data

SheetReadthelabelsonallreagentbottles.Notewarningsandfirstaidinformation.ReadallMaterialSafetyDataSheets.

3. 4.

5. 6.

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USE PROPER ANALYTICAL TECHNIQUES

Usetesttubecapsorstoppers,notyourfingers,tocovertubesduringshakingormixing.

Tightlycloseallcontainersimmediatelyafteruse.Donotinterchangecapsfromcontainers.

1.

Avoidprolongedexposureofequipmentandreagentstodirectsunlight.Protectreagents

from

extremesoftemperature.

2.

Wipeupanyreagentchemicalspillsimmediately.3. 4.

5. 6.

Holddropperbottlesverticallyupside-down,andnotatanangle,whendispensingareagent.Squeezethebottlegentlytodispensethereagentonedropatatime.

Thoroughlyrinsetesttubesbeforeandaftereachtest.

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KIT DIAGRAMS

DISSOLVED OXYGEN KIT CODE 7414

Instructions4169-H

0377

6286-H

4167-G

4170WT-G 0608Tube

7166-G0688-DO

0697

DISSOLVED OXYGEN KIT CODE 5860

Instructions4169-H

0377

6141WT-G

4167-G

4170WT-G 0608Tube

7166-G0688-DO

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SHORT FORM INSTRUCTIONS

Readallinstructionsbeforeperformingtest.Usethisguideasaquickreference.1. FillWaterSamplingBottle(0688-DO).2. Add8dropsof*ManganousSulfateSolution(4167).3. Add8dropsof*AlkalinePotassiumIodideAzide(7166).4. Capandmix.5. Allowprecipitatetosettle.6. Usethe1.0gspoontoadd*SulfamicAcidPowder(6286)or

add8dropsofSulfuricAcid,1:1(6141WT).7. Capandmixuntilreagentandprecipitatedissolve.8. Filltesttube(0608)tothe20mLline.9. FillTitratorwith*SodiumThiosulfate,0.025N(4169).10. Titrateuntilsamplecolorispaleyellow.DONOTDISTURB

TITRATOR.11. Add8dropsofStarchIndicator(4170WT).12. Continuetitrationuntilbluecolorjustdisappearsandsolution

iscolorless.13. ReadresultinppmDissolvedOxygen.

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LaMotte 5860 Dissolved Oxygen Liquid Acid Version Kit Instructions

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